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Citations for New England Biolabs :
301 - 350 of 1043 citations for Recombinant Human Complement Factor H His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... pCAV-FLExloxP-Flp was incubated with recombinant Cre recombinase (New England Biolabs) and then transformed into DHα bacteria ...
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bioRxiv - Neuroscience 2022Quote: ... the recombinant proteins were purified by amylose resin (#E8022S, New England Biolabs) according the manual instructions ...
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bioRxiv - Synthetic Biology 2019Quote: ... and 0.4 µL (16 U) of recombinant RNase inhibitor (New England Biolabs) in a final volume of 20 µL ...
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bioRxiv - Synthetic Biology 2019Quote: ... and 0.75 µL (30 U) of recombinant RNase inhibitor (New England Biolabs) in a final volume of 30 µL ...
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bioRxiv - Synthetic Biology 2021Quote: The recombinant CBX8-CD was expressed in BL21 (DE3) (New England Biolabs) Escherichia coli cells ...
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bioRxiv - Biophysics 2021Quote: ... Recombinant M-MuLV reverse transcriptase from the ProtoScript® II kit (NEB) was used to synthesize first strand cDNA from the annealed primer-MS2 RNA mix ...
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bioRxiv - Cell Biology 2020Quote: Recombinant GFP-fusion proteins were purified from BL21(DE3) cells (NEB, # C2527H) using an N-terminal H6-tag ...
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bioRxiv - Biochemistry 2022Quote: The recombinant Lili-Mips were treated with PNGase F (New England Biolabs) to remove the N-linked oligosaccharides ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant vectors were generated using HiFi DNA Assembly Cloning Kit (NEB E5520). During cloning procedures ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant vectors were constructed using HiFi DNA Assembly Cloning Kit (NEB E5520). electroporation (device) ...
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bioRxiv - Biochemistry 2020Quote: 20 μg of protein from SK-RC-39 cells were treated with N-glycosidase F (PNGase F) and Endoglycosidase H (Endo H) (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... for 6 h at 37°C in the absence or presence of RNase H or RNase A (40 U, NEB). Digested nucleic acids were cleaned with phenol-chloroform extraction and resuspended in nuclease-free water ...
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bioRxiv - Biochemistry 2021Quote: ... purified RNA was dissolved in 3 μl RNase H reaction mix (1x RNase H buffer [NEB], 40 pmol oligo(dT)12 ...
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bioRxiv - Biochemistry 2021Quote: 30 to 50 μg proteins were digested for 90 min at 37°C with endoglycosidase-H (Endo-H; P0702L) or endoglycosidase-F (Endo-F; P0705S) as recommended by the manufacturer (New England Biolabs).
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bioRxiv - Microbiology 2022Quote: ... The relative abundance of oligomannose-type glycans was measured by digestion with Endoglycosidase H (per sample in 20 µl volume) (Endo H; New England Biolabs). A 6 µl aliquot of labelled glycans was combined with 1 µg endoH to a final volume of 20 µl ...
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bioRxiv - Microbiology 2023Quote: ... On-bead RNase H treatment control was performed for each reaction: washed beads were incubated with 300 µL 1X RNase H Buffer (NEB) plus 30 units of RNase H (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... or MnlI was at 37 °C for 2 h (EcoRI-HF) or 3 h (HhaI or MnlI) in rCutSmartTM Buffer (NEB). Nuclease S1 (Boehringer Mannheim ...
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bioRxiv - Microbiology 2024Quote: ... according to manufacturer’s instructions using 100 nM RNA-specific reverse transcription primer followed by RNase H digest with 5 µL RNase H (NEB) at 37 °C for 20 min ...
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bioRxiv - Synthetic Biology 2020Quote: ... The cloning was performed with the NEBuilder Hi Fi DNA Assembly kit (New England Biolabs). The enzymes mentioned were purchased from New England Biolabs ...
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bioRxiv - Immunology 2023Quote: In situ Hi-C experiments were performed as previously described2 using restriction enzyme MboI (NEB) with minor modifications ...
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bioRxiv - Genomics 2023Quote: ... pooled oligonucleotides were amplified via PCR using NEBNext 2X Hi-Fi PCR Master Mix (NEB) and primers:
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bioRxiv - Microbiology 2023Quote: ... His/Strep-Tag was cleaved using enterokinase according to the protocol of the manufacturer (NEB) and GlnA3Mtwas immediately purified from the digestion mix by size-exclusion chromatography as directed by the resin manufacturer (GE-Healthcare).
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bioRxiv - Cancer Biology 2024Quote: ... Hi-C libraries were treated with T4 DNA Polymerase (New England Biolabs, catalog No. M0203) to remove biotin from unligated ends ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 µl RNase H (5U final, NEB M0297L) was added directly to each reaction and incubation for 20 min at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... the lysates were treated with endoglycosidase H (NEB).
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bioRxiv - Cancer Biology 2021Quote: ... Residual RNA was degraded by RNAse H (NEB) digestion ...
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bioRxiv - Microbiology 2020Quote: ... and Endo H (New England Biolabs, Ipswich, MA), following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... or treated with Endoglycosidase H (EndoH, NEB, P0702S) or N-glycosilase F (PNGaseF ...
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bioRxiv - Molecular Biology 2020Quote: ... and 50 U RNase H (New England Biolabs), and incubated at 37°C for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... Two units per microliter of RNAse H (NEB) was added to remove RNA and samples were incubated at 37°C for 20 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Residual RNA was degraded with RNAse H (NEB), and cDNA was poly(A ...
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bioRxiv - Cell Biology 2020Quote: ... 1μL of Endoglycosidase H (EndoH; New England BioLabs) was added to the other sample ...
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bioRxiv - Cancer Biology 2021Quote: ... Residual RNA was degraded by RNAse H (NEB) digestion and poly(A ...
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bioRxiv - Microbiology 2022Quote: ... 2 µL Thermostable RNase H (NEB, CN E7850), and 1 µL nuclease-free H2O ...
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bioRxiv - Molecular Biology 2023Quote: ... 1.25 U of RNase H (New England Biolabs) and 12.5 U of RNase If (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... RNAse H and DNA polymerase (New England Biolabs) and purified using Agencourt AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Cell Biology 2023Quote: ... fragmented DNA was treated with RNAse-H (NEB) for 4-6 h at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: ... the samples were treated with Endo H (NEB) and GlycoBuffer (NEB ...
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bioRxiv - Immunology 2023Quote: ... 2μl of endo H (1000U) (New England Biolabs) or 2μl of PBS (mock ...
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bioRxiv - Cell Biology 2023Quote: ... and 0.2 U/μl RNAse H (NEB #M0297) and immediately used for the overhang assay as follows ...
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bioRxiv - Molecular Biology 2024Quote: ... gDNA was treated with RNase H (NEB, M0297S) (5 U per µg of gDNA ...
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bioRxiv - Cancer Biology 2024Quote: ... and/or RNase H (New England Biolabs, M0297S) and incubated with rocking for 1 hr ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µL RNase H (5 U/µL, NEB), 2.5 µL RNase III (1 U/µL ...
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bioRxiv - Immunology 2024Quote: ... 2μl of endo H (1000U) (New England Biolabs) or 2μl of PBS (mock ...
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bioRxiv - Developmental Biology 2021Quote: ... and amplified/tagged in two steps using NEBnext High-Fidelity 2x PCR master mix (New England Biolabs). Amplified DNA was purified twice with 1.8 volumes of NucleoMag NGS Clean-up and Size Select beads (Macherey Nagel) ...
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bioRxiv - Biophysics 2020Quote: mNG-tagged ADRβ2 and EGFR were generating by cloning into the pSNAPf-ADRβ2 backbone (New England Biolabs). mNG was amplified by PCR from mNG-C1 and placed between the EcoRI and SbfI sites of pSNAPf-ADRβ2 (replacing the SNAP tag ...
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bioRxiv - Cell Biology 2021Quote: ... 25ug of fragmented DNA was then equilibrated to 1X RNase-H buffer and treated with 50 units of RNase-H (NEB, M0297L) for 3h at 37°C ...
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bioRxiv - Genomics 2019Quote: ... the temperature was slowly ramped down (−0.1°C/sec) to 45°C (for Hybridase RNase H) or 22°C (for NEB RNase H), and the mixture was incubated at 45°C (for Hybridase RNase H ...
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bioRxiv - Microbiology 2022Quote: ... Purified protein (4 μg) was deglycosylated with 500 U of Endoglycosidase H (Endo H) (New England Biolabs, Ipswich, MA, United States) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... Lysates were first denatured with Glycoprotein Denaturing Buffer at 65°C for 15 min and then treated with Endoglycosidase H (Endo H) (New England Biolabs, #P0702S) or Peptide-N-Glycosidase F (PNGase F ...