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Citations for New England Biolabs :
151 - 200 of 1043 citations for Recombinant Human Complement Factor H His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... For all Gibson Assemblies the NEB Hi-Fi Assembly Mastermix (NEB) was used ...
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bioRxiv - Genetics 2023Quote: ... To facilitate homology directed assembly (Gibson or NEB Hi Fi assembly), 30bp homology with the preferred SEED cassettes were included at each site of the BsaI sites ...
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bioRxiv - Synthetic Biology 2023Quote: ... For his purpose pEKEx2 was first linearized using SacI (NEB, USA). The gene for mCherry was amplified by PCR using the primer pairs low_mCherry_fw and low_mCherry_rev (Table S1 ...
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bioRxiv - Biochemistry 2021Quote: ... MBP was cleaved from LH using Factor Xa (New England Biolabs) at a w/w ratio of 1% Factor Xa:LH ...
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bioRxiv - Molecular Biology 2021Quote: ... 200 units RNase H (NEB) and 20 μl of SUPERasIN (ThermoFisher) ...
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bioRxiv - Genetics 2021Quote: ... RNAse H (5 Units, NEB), rSAP (1 Unit ...
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bioRxiv - Immunology 2021Quote: ... For Endoglycosidase H (EndoH, NEB) digestions ...
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bioRxiv - Cell Biology 2020Quote: ... For Endo H (NEB, P0702L) treatment ...
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bioRxiv - Genomics 2021Quote: ... coli RNase H (NEB M0297S). The mixture was incubated at 37 °C for 30 minutes after which the enzyme was inactivated using 10 mM EDTA ...
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bioRxiv - Genomics 2021Quote: ... coli RNase H (NEB M0297S). The mixture was incubated at 37 °C for 30 minutes after which the enzyme was inactivated using 10 mM EDTA ...
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bioRxiv - Molecular Biology 2022Quote: ... ii) 500U Endo H (NEB), or iii ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 1uL RNase H (NEB) were added and the mixture was incubated at 37C for 15 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... For RNase H (NEB # M0297S) or RNase A (Sigma #R6148 ...
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bioRxiv - Molecular Biology 2023Quote: ... or RNase H (NEB M0297) at 37C ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 μl RNase H (NEB), and 70 μl nuclease-free H2O to the cDNA mixture and incubation at 37 °C for 15 min ...
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bioRxiv - Microbiology 2024Quote: ... RNase H (NEB, cat.m0297, USA) and dUTP Solution (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: Myc- and DDK (FLAG)-tagged Hemopexin mRNA was transcribed from an AgeI (NEB) linearised plasmid using T7 RNA polymerase (Promega) ...
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bioRxiv - Plant Biology 2022Quote: ... Each MBP-tagged RAPTOR1B fragment was detected with anti-MBP antibodies (NEB, E8032S). For the in vitro kinase assay ...
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bioRxiv - Microbiology 2022Quote: ... and tagged for multiplexing via the NE Next Multiplex Oligos for Illumina (NEB).
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bioRxiv - Molecular Biology 2022Quote: ... The HA-tagged PTBP1 was inserted into pcDNA3 between the EcoRI (NEB, R3101S) and NotI (NEB ...
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Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: ... The MBP tagged proteins were purified by using Amylose Resin (New England Biolabs) and Ultrafiltration tubes (Millipore ...
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bioRxiv - Genomics 2021Quote: ... The purified recombinant protein or commercial M.EcoGII (NEB, M0603S) was diluted with coating buffer (0.05 M NaHCO3 buffer ...
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bioRxiv - Biochemistry 2021Quote: ... This experiment was further repeated with recombinant Histones (NEB) (0.5µg ...
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bioRxiv - Molecular Biology 2023Quote: ... a combination of both recombinant RBPMS and BSA (NEB). Splicing reactions were incubated for the 3 hr ...
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bioRxiv - Molecular Biology 2023Quote: ... 20pmol of recombinant histone H3.1-H4 tetramer (NEB, M2509S) were immediately added to the tube ...
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bioRxiv - Cell Biology 2020Quote: ... RNase H treatment on fixed cells was performed using 5 U RNase H (M0297, NEB) for 3 h at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were then incubated with RNase H (NEB, 1: 50 in 1X RNase H buffer) at 37°C for 4 hours ...
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bioRxiv - Genetics 2019Quote: ... Hi-C DNA was amplified using Index Primers set 1 (NEB, E7335S). The Hi-C libraries were purified with AMPure XP beads (Beckman Coulter ...
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bioRxiv - Immunology 2021Quote: ... and the fragment was assembled using Hi-Fi DNA Assembly Mix (NEB) into the donor vector pADN171XS7 ...
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bioRxiv - Microbiology 2021Quote: ... His-MBP-MirA were first isolated using amylose bead affinity chromatography (NEB) in conditions specified by the manufacturer ...
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bioRxiv - Plant Biology 2021Quote: ... The homology plasmids were assembled using HI-Fi DNA Assembly Mastermix (NEB) with four PCR-generated DNA fragments (see Table S2 for primers) ...
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bioRxiv - Molecular Biology 2019Quote: ... purified and then cleaved with Xho I and Bam HI (both NEB). After further purification ...
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bioRxiv - Microbiology 2021Quote: ... Then the His-tag was cleaved by incubation with TEV protease (NEB) for 18 h at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... and then ligated by Hi-Fi DNA assembly (New England Biolabs Inc.). Irgb6-WT and Irgb6-T95D were expressed and purified as described (Saijo-Hamano et ...
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bioRxiv - Biochemistry 2023Quote: ... was cloned into pUC19 by Gibson cloning (Hi-Fi cloning system, NEB) to replace the yeast sequence:
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bioRxiv - Biophysics 2021Quote: MukF Flag-tagged fragments were expressed from pET 21 plasmids in C3013I cells (NEB).The Flag-tagged MukE and MuF were at the C-terminus ...
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bioRxiv - Cell Biology 2021Quote: ... MBP-tagged fragments were purified by gravity flow through amylose resin (New England Biolabs) and step elution in maltose ...
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bioRxiv - Biochemistry 2023Quote: ... containing a His6-tagged Ulp1 gene was transformed into T7 Express competent cells (NEB) and grown in LB medium supplemented with ampicillin (100 μg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... MBP-tagged CCDC22 and CCDC93 proteins were purified using Amylose beads (New England Biolabs), mixed in approximately 1:1 stoichiometry ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were treated with 60 U/ml of ribonuclease H (RNase H, 5.000 units/ml, NEB) using RNase H buffer at 37°C for 1 hour ...
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bioRxiv - Cancer Biology 2022Quote: ... in RNase H buffer (NEB, M0297) overnight at 37 °C ...
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A universal fluorescence-based toolkit for real-time quantification of DNA and RNA nuclease activitybioRxiv - Biochemistry 2019Quote: ... and RNase H (New England Biolabs). In preparation for each assay ...
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bioRxiv - Bioengineering 2020Quote: ... 0.1 U/µl RNase H (NEB), 120 nM forward and reverse RPA primers ...
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bioRxiv - Biochemistry 2021Quote: ... 10 U of RNase H (NEB) was added ...
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bioRxiv - Biochemistry 2020Quote: ... Endo H (P0702L, New England Biolabs), neuraminidase (10269611001 ...
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bioRxiv - Molecular Biology 2021Quote: ... or Endoglycosidase H (New England Biolabs) was added to 40μL of cleared lysate and incubated at 37° for 2 hours ...
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bioRxiv - Microbiology 2020Quote: ... 2U/μl of RNAse H (NEB) was added to remove RNA and samples were incubated at 37°C for 20 min ...
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bioRxiv - Biochemistry 2022Quote: ... coli RNase H (New England Biolabs) or T ...
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bioRxiv - Immunology 2022Quote: ... RNase H (both New England Biolabs) and dUTP Solution (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... 2U/µl of RNAse H (NEB) was added to remove RNA and samples were incubated at 37°C for 20 min ...