Labshake search
Citations for New England Biolabs :
301 - 350 of 3846 citations for Human Chemokine C X C Motif Receptor 5 CXCR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... while the other was digested at 25 °C with 10 units SwaI (New England Biolabs). Enzymes were inactivated at 65 °C for 20 min ...
-
Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: ... Samples were cooled to 42 °C and 2 μl of β-agarase I enzyme (NEB) was added and incubated at 42 °C for 90 min with occasional vortexing ...
-
bioRxiv - Cell Biology 2023Quote: ... at 37 °C for 1 hour prior to the addition of T4 DNA ligase (NEB). The ligation reaction was incubated at room temperature for 1 hour then transformed into DH5ɑ competent cells.
-
bioRxiv - Microbiology 2024Quote: ... 72°C: 1’0 min) with a Q5 high fidelity DNA polymerase (New England Biolabs, M0491) and 10 µM forward gene specific primers also containing an additional UMI sequence (8 random nucleotides ...
-
bioRxiv - Cell Biology 2024Quote: ... G or C) and assembled using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs). The randomized N12-oligonucleotides were commercially synthesized and purified on a reversed-phase column (FASMAC ...
-
bioRxiv - Molecular Biology 2024Quote: ... and fragmentation (25 min at 94 °C in 2X T4 PNK reaction buffer from NEB –140 mM Tris-HCl pH 7.6 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Plugs were melted at 70°C and treated with ý-Agarase I (NEB, catalog #M0392) to liberate the DNA ...
-
bioRxiv - Biochemistry 2024Quote: Constructs bearing a C-terminal cMyc tag were cloned by site-directed mutagenesis (NEB, M0554S) using oligonucleotides as primers bearing a 5’-overhang encoding the inserted sequence ...
-
bioRxiv - Neuroscience 2023Quote: ... C-sbGluc and p2a dTomato by Gibson cloning (New England Biolabs HiFi DNA Master Mix). Coding sequences from plasmids for GCaMP6f ...
-
bioRxiv - Genomics 2024Quote: ... pX458 and pX459 vectors were previously digested 2hours at 37°C by BbsI (NEB, R0539) and dephosphorylated 10 minutes at 37°C using Quick CIP (NEB ...
-
bioRxiv - Cancer Biology 2024Quote: ... Hi-C libraries were treated with T4 DNA Polymerase (New England Biolabs, catalog No. M0203) to remove biotin from unligated ends ...
-
bioRxiv - Molecular Biology 2022Quote: ... The G4 sequence and larger 5′ UTR (three human β-globin 5′ UTR sequences in tandem) were inserted using the Q5 Site-Directed Mutagenesis Kit (NEB # E0552S). pcDNA3.1(+)/mEGFP was a kind gift from Jeremy Wilusz (Baylor College of Medicine) ...
-
bioRxiv - Molecular Biology 2019Quote: ... 5 µl of a broad range protein marker (New England Biolabs, #P7708) was run alongside the samples on each gel to estimate the molecular weight ...
-
bioRxiv - Microbiology 2023Quote: ... 5 μL of 2X protein synthesis buffer (New England Biolabs, Beverly, MA), 0.25 μL of RNaseOUT RNAse inhibitor (Thermo-Fisher ...
-
bioRxiv - Cell Biology 2020Quote: ... samples were cooled and then digested with DpnII for one hour at 37° C (NEB, R0543). These ligated pools were then amplified using AdR_PCR oligonucleotides as primer (5′-GGTCGCGGCCGAGGATC-3′ ...
-
bioRxiv - Microbiology 2021Quote: ... Supernatants were thawed at 37°C and mixed with 20 µL of a 500U DNaseI (NEB), 10X buffer and nuclease free water mixture and incubated at 37°C for 30 minutes ...
-
bioRxiv - Molecular Biology 2020Quote: ... were incubated for 12 hours at 30°C with 7.5 Units of Phi29 polymerase (NEB M0269) in Phi29 NEB buffer 1X ...
-
bioRxiv - Systems Biology 2019Quote: ... 30°C and the growth was continuously monitored online using Cell Growth Quantifier (Aquila Biolabs GmBH). Samples were withdrawn at regular intervals for analyzing the spent metabolites through High-Performance Liquid Chromatography (HPLC).
-
bioRxiv - Microbiology 2019Quote: ... which was placed towards the centre of a C760 Innova – 70 °C freezer (New England Biolabs) for 1 h before being thawed in a 37 °C waterbath for approximately two min.
-
bioRxiv - Molecular Biology 2020Quote: ... After incubation for min at 55°C 1 μl T4 ligase (400,000 units/ml, NEB, M0202) was added ...
-
bioRxiv - Genetics 2019Quote: The product was digested overnight at 37°C using 20 U PmlI (New England Biolabs, USA), 5 uL accompanying buffer ...
-
bioRxiv - Microbiology 2019Quote: ... and 4 μL was ligated at 16°C overnight with the T4 DNA ligase (NEB M0202S). After transformation into chemically-competent Top10 E ...
-
bioRxiv - Cell Biology 2020Quote: ... pH 8.6) overnight at 37 °C in the presence of non-tritiated SAM (New England Biolabs). Samples were resolved via SDS-PAGE ...
-
bioRxiv - Genomics 2019Quote: ... Tagmented DNA was End-Repaired for 30 minutes at 37°C (5U Klenow Exo-(NEB #M2012S), 1 × NEB Buffer 2 ...
-
bioRxiv - Bioengineering 2022Quote: ... Samples were stored at -20° C before PCR amplification of cDNA with Q5 (New England Biolabs) or SuperFiII (ThermoFischer).
-
bioRxiv - Molecular Biology 2022Quote: ... and were ligated over night at 16 °C using T4 DNA ligase (New England Biolabs, UK). The ligation mix was transformed into E ...
-
bioRxiv - Microbiology 2021Quote: ... after ligation overnight at 4°C with the T4 DNA ligase (New England Biolabs, Evry, France).The sequences of the cloned fragments in the resulting plasmids pYES2::MlpCSP1 ...
-
bioRxiv - Microbiology 2021Quote: ... immediately rescued in 1 ml of 37°C SOC outgrowth medium (New England Biolabs, cat#B9020S) and incubated with shaking at 37°C for 1 hour ...
-
bioRxiv - Neuroscience 2021Quote: ... PCR products were incubated overnight at 60 °C with either BstNI restriction enzyme (New England Biolabs) or a control buffer solution without enzyme ...
-
bioRxiv - Molecular Biology 2022Quote: ... and were ligated over night at 16 °C using T4 DNA ligase (New England Biolabs, UK). The ligation mix was transformed into E ...
-
bioRxiv - Microbiology 2022Quote: ... Samples were treated twice for 45 minutes at 37°C with DNase I (New England Biolabs) with heat inactivation at 75°C for 10 minutes and DNA was further removed using a RNeasy Mini kit (Qiagen) ...
-
bioRxiv - Microbiology 2019Quote: ... Individual fragments were amplified at 60°C with Phusion® High-Fidelity Polymerase (New England Biolabs) using T ...
-
bioRxiv - Physiology 2021Quote: ... The pNB1u vector was digested overnight at 37 °C with PacI and Nt.BbvCI (New England Biolabs) to generate 8-nt overhangs ...
-
bioRxiv - Biochemistry 2019Quote: ... 4°C) and the supernatant was added to 24 mL of amylose resin (NEB, Ipswich MA) pre-equilibrated with buffer C and nutated for 2 hr at 4°C ...
-
bioRxiv - Genomics 2021Quote: ... followed by an overnight incubation at 16°C with 3 µL T4 DNA ligase (NEB, M0202). Samples were purified with phenol-chloroform and used as 3C templates for Taqman-qPCR ...
-
bioRxiv - Biochemistry 2020Quote: ... Reaction mixtures were treated with proteinase K at 37 °C for 10 min (New England Biolabs) and the RNA samples were purified by RNeasy Mini Kit (Qiagen) ...
-
bioRxiv - Genetics 2021Quote: ... for 1 hour at 37°C into pPB_U6::gRNA_EF1a::BFP-Puro digested with BlpI (NEB #R0585S) and BstXI (NEB #R0113S) ...
-
bioRxiv - Genomics 2019Quote: ... samples were cooled and then digested with DpnII for one hour at 37°C (NEB, R0543). These ligated pools were then amplified using AdR_PCR oligonucleotides as primer (5′ -GGTCGCGGCCGAGGATC-3′ ...
-
bioRxiv - Neuroscience 2020Quote: ... After overnight incubation at 4°C in PBS-Perm with rabbit anti-GFP (1:1000, Biolabs) and mouse anti-MAP2 (1:2000 ...
-
bioRxiv - Cell Biology 2020Quote: ... pFA6a-mto2S338N-C-KanMX6 was generated using NEBuilder HiFi DNA Assembly Master Mix (New England BioLabs) in an assembly containing 5 fmol of each fragment ...
-
bioRxiv - Biochemistry 2022Quote: In vitro coupled transcription-translation was performed at 37 °C using the PURExpress ΔIleRS Kit (NEB) following manufacturer’s protocol supplemented with [35S]-methionine (EasyTag ...
-
bioRxiv - Neuroscience 2022Quote: ... Plasmid and insert were then ligated overnight at 16 °C (T4 DNA ligase, New England Biolabs). After transformation into chemically competent E.coli and subsequent plasmid purification ...
-
bioRxiv - Plant Biology 2023Quote: ... 10 ul of diluted DNA were used for McrBC digestion (NEB, 4 h at 37 °C) or mock digestion (the same volume of H2O instead of McrBC enzyme was added with all other components the same in the reaction ...
-
bioRxiv - Molecular Biology 2022Quote: ... for 2 hours at 37°C in 1X T4 PNK buffer (New England Biolabs, cat#B0201S). Radioactively labeled tRNAs were produced by T7 in vitro transcription in the presence of [α-32P]-UTP (PerkinElmer ...
-
bioRxiv - Genomics 2022Quote: ... cells were digested for 2 hours at 37°C using 375U of MboI (NEB, Cat. #R0147). After biotin filling ...
-
bioRxiv - Microbiology 2023Quote: ... Vector and insert were ligated overnight at 16°C using T4 DNA ligase (New England Biolabs) and transformed into E ...
-
bioRxiv - Cancer Biology 2023Quote: ... Hi-C single-index library preparation was performed as previously described56 using MboI (New England Biolabs) restriction enzyme.
-
bioRxiv - Microbiology 2023Quote: ... Total RNA was then digested using DNase I for 2h at 37°C (New England Biolabs) to remove residual plasmid DNA and then purified using Monarch RNA cleanup kit (New England Biolabs) ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... and the P1-adaptors and Sbf I-HF (New England Biolabs)-digested DNAs for 90 min at 37°C were ligated by T4 ligase (New England Biolabs). After DNA pooling ...
-
bioRxiv - Cell Biology 2023Quote: ... with C-terminal fluorescent tags using seamless cloning (HiFi DNA Assembly Master Mix, New England Biolabs). The pLVX-KRT5-mNG-IRES-Puro construct includes an mNeonGreen (Allele Biotechnology)6 sequence in-frame with the KRT5 sequence separated by a short peptide linker (DPAFLY) ...