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Citations for New England Biolabs :
151 - 200 of 3846 citations for Human Chemokine C X C Motif Receptor 5 CXCR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... 37°C with vaccinia virus capping enzyme (New England Biolabs), as per the manufacturer’s protocol ...
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bioRxiv - Biophysics 2022Quote: ... 1 µL of 0.4 µg µL-1 Lys-C (NEB) stock was added (enzyme:substrate ratio of 1:100 w/w ...
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bioRxiv - Neuroscience 2023Quote: ... 4°C) and filled in using T4 DNA polymerase (NEB) using manufacturers’ instructions at 12°C for 20 min75 ...
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bioRxiv - Plant Biology 2024Quote: ... and ligated overnight at 16°C (T4 DNA ligase; NEB). The same approach was used for the second deletion (353 bp ...
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bioRxiv - Bioengineering 2023Quote: ... and at 37 °C for MscI (New England Biolabs, R0534) and BstEII-HF (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: ... The reaction was slowly cooled down to room temperature with a Δ -1°C / second gradient and 0.5 μl of each RNase H (NEB, 5 U/μl), RNase T1 (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... Processing the samples for proteomics was then continued using a 10 kDa filter aided sample preparation protocol previously published with minor modifications.[30] The proteins were digested using Lys-C enzyme (P8109S, New England Biolabs, MA, USA; 1:50) and then with trypsin (V5111 ...
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bioRxiv - Genetics 2021Quote: ... 5 μg of MBP-Rif2 or MBP-Rif2-min (see recombinant protein preparation) was incubated for 1 h at 4 °C with amylose resin (New England Biolabs, 50 μl per reaction). The resin with the immobilized MBP-Rif2 variants was washed 5 times with 1 ml wash buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Plant Biology 2024Quote: ... when OD600 reached 0.5 for 4 h at 37 °C and then they were purified using amylose resin column (New England Biolabs, for MBP related proteins purification) or Ni-NTA agarose column (Qiagen ...
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bioRxiv - Genetics 2020Quote: ... Only 1 U/μl I-SceI enzyme 5 X/μl buffer (NEB) were mixed when co-injecting with the HDR donor ...
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bioRxiv - Cell Biology 2020Quote: ... Chromatin was digested with Microccocal Nuclease (NEB, 37°C, 30 min) in digestion buffer (1M Tris-HC ...
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bioRxiv - Molecular Biology 2021Quote: ... and 72°C for 30 sec) with Q5 polymerase (NEB Inc.) and appropriate primers (S2 Table).
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bioRxiv - Developmental Biology 2020Quote: ... for 30□min in 37°C and proteinase K (NEB, P8102S) for 2□h in 37°C and de-crosslinked in 65□°C for 8□h ...
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bioRxiv - Developmental Biology 2022Quote: ... 72°C for 1 min)) using NEBNext 2x Master Mix (NEB) and Nextera adapters and put on ice ...
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bioRxiv - Microbiology 2019Quote: ... were digested for 1.5 hrs at 37°C with NotI (NEB) to introduce the bla gene at the unique NotI site of pPEPZ-Plac (spec) ...
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bioRxiv - Biochemistry 2020Quote: ... samples were digested overnight at 37°C with PNGase F (NEB). After the N-linked glycans were removed ...
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bioRxiv - Plant Biology 2021Quote: ... Digestions were performed overnight at 37°C with 400U DpnII (NEB). DNA was ligated by incubation at 16°C for 5h in 4 ml volume using 100U of T4 DNA ligase (NEB) ...
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bioRxiv - Molecular Biology 2022Quote: ... at 37°C overnight and then 100ng GluC (New England BioLabs) was added for another overnight incubation at 25°C ...
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bioRxiv - Biophysics 2019Quote: ... for 15 minutes at 12°C in 1x buffer 2.1 (NEB), to remove 3’-A overhangs.
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bioRxiv - Molecular Biology 2021Quote: ... 7 units USER Enzyme for 30 minutes at 37°C (NEB)51 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Mixes were subsequently digested 1h at 37 °C with DpnI (NEB) and used for electroporation in E ...
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bioRxiv - Genetics 2019Quote: ... for 1 hr at 37°C followed by Exonuclease T (NEB) for 45 min at 24°C to blunt DNA ends before Illumina adapter ligation (Canela et al. ...
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bioRxiv - Plant Biology 2023Quote: ... Digestions were performed overnight at 37°C with 400U DpnII (NEB). DNA was ligated by incubation in a shaker at 16°C ...
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bioRxiv - Molecular Biology 2024Quote: ... and 72°C for 30 sec) with Q5 polymerase (NEB Inc.) and appropriate primers (Supp ...
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bioRxiv - Cell Biology 2023Quote: ... for 1 hour at 37°C in 1x ThermoPol buffer (NEB). Samples were processed and analyzed as described15.
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bioRxiv - Developmental Biology 2023Quote: ... 72°C for 1 min) using NEBNext 2x Master Mix (NEB) and Nextera adapters and put on ice ...
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bioRxiv - Biochemistry 2020Quote: ... or a control motif (5’-GGGACCCTGGGAGGG-3’) were prepared by viral replication using a helper phage M13K07 (New England BioLabs, Cat#N0315S). E.coli XL1-Blue cells were transformed with pBluescript SK(- ...
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bioRxiv - Synthetic Biology 2021Quote: ... for 4 hours at 37°C and treated with Antarctic phosphatase (NEB) for 30 min at 37°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 4: 4°C hold) using NEB Next High-Fidelity master mix (NEB) in 25 µl reaction volume using RAD-Marker for/ RAD-Marker rev primers (25 nM each ...
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bioRxiv - Microbiology 2022Quote: ... at 16°C overnight or Gibson Assembly Master Mix (New England Biolabs) per manufacturer protocols where stated ...
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bioRxiv - Systems Biology 2019Quote: ... 72°C for 30s) using Illumina indexing primers (NEB Catalog No. 7500L). DNA was purified using Ampure XP beads and sequenced on Illumina HiSeq 2500 to generate 75 bp paired-end reads ...
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bioRxiv - Genetics 2019Quote: ... Hi-C DNA was amplified using Index Primers set 1 (NEB, E7335S). The Hi-C libraries were purified with AMPure XP beads (Beckman Coulter ...
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bioRxiv - Cancer Biology 2019Quote: ... XbaI or XhoI or 50°C with SfiI (NEB Ipswich, MA, USA). The ligations were performed 30 min at room temperature using a T4 DNA ligase (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... the C-terminal domain was removed by digestion with Endoproteinase LysC (NEB) at a 1:800 LysC:substrate ratio by mass for 1-2h at 37°C ...
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bioRxiv - Biophysics 2019Quote: ... for one h at 37 °C in the CutSmart™ buffer (NEB). After that ...
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bioRxiv - Microbiology 2022Quote: ... for 7-12 h at 37°C in CutSmart buffer (NEB, B7204S). Digested products were then visualised on a 4% NuSieve (Lonza ...
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bioRxiv - Microbiology 2023Quote: ... and M.Fnn25V for two hours at 37 °C in CutSmart buffer (NEB) containing 160 μM S-adenosylmethionine (SAM ...
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bioRxiv - Molecular Biology 2023Quote: ... Both were ligated at 16°C overnight with T4 DNA ligase (NEB) before XL1 blue transformation ...
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bioRxiv - Molecular Biology 2023Quote: ... at 37 °C for 2 hours followed by digestion with PspXI (NEB) in rCutsmart™ Buffer (NEB ...
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bioRxiv - Genomics 2024Quote: ... and dephosphorylated 10 minutes at 37°C using Quick CIP (NEB, M0525). The vectors were then purified with PCR CleanUp kit (Macherey Nagel ...
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bioRxiv - Molecular Biology 2023Quote: ... from 4 to 15 hours at 37 °C. For Circle-Seq of the CRISPR treatments and controls (Fig. 5) exonuclease T (New England Biolabs; 20 units per μg of DNA) was then added ...
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bioRxiv - Immunology 2022Quote: ... Control EGFR and EGFR-CXCR5 were generated by removing the CER from the above construct using the NEBuilder HiFi DNA Assembly (New England Biolabs). CER 104 and CER 131 were generated by adding the human DAP 12 signaling domain ( GenBank™ accession number NP_001166985 ...
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bioRxiv - Genomics 2023Quote: ... 0.5 uL of 5 x mRNA Second Strand Synthesis buffer (New England Biolabs, E6111L), and 0.25 uL of mRNA Second Strand Synthesis enzyme (New England Biolabs ...
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bioRxiv - Neuroscience 2023Quote: ... 0.5 uL of 5 x mRNA Second Strand Synthesis buffer (New England Biolabs, E6111L), and 0.25 uL of mRNA Second Strand Synthesis enzyme (New England Biolabs ...
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bioRxiv - Genetics 2021Quote: ... then a final 1 min extension (72 °C)—using Phusion Flash polymerase (NEB) from cDNA clones using primers containing an EagI restriction site (forward primers ...
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bioRxiv - Developmental Biology 2021Quote: ... Restriction digest at 37°C for 20 minutes with Msp1 (New England Biolabs) was performed to cleave the wildtype ...
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bioRxiv - Developmental Biology 2020Quote: ... Chromatin was digested overnight at 37°C by DpnII (New England Biolabs,. R0543M). The digestion was monitored by specific primers spanning DpnII sites in the genome (Supplemental Table 3)
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bioRxiv - Microbiology 2020Quote: ... incubated overnight at 4°C and treated with DNAse I (New England Biolabs) at 37°C for 60 minutes ...
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bioRxiv - Genomics 2020Quote: ... and then held at 4°C until a subsequent T7 Endonuclease I (NEB) treatment at 37°C for 30 to 90 minutes ...
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bioRxiv - Genomics 2021Quote: ... for 1 hour at 37°C in the presence of CutSmart buffer (NEB). To achieve combined DNA purification and selection of high molecular weight fragments ...