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Citations for New England Biolabs :
301 - 350 of 4002 citations for Hexadecanoic acid reaction products with tetraethylenepentamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... The PCR product was digested with NotI (NEB, R3189L) and EcoRI (NEB ...
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bioRxiv - Microbiology 2024Quote: ... 5 μl vaccinia capping enzyme (NEB product number M2080), 5 μl inorganic pyrophosphate (New England Biolabs product number M2403) ...
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bioRxiv - Developmental Biology 2024Quote: ... then PCR products were cut with BccI enzyme (NEB) which cleaved the wild-type amplicon into two products (120 bp and 76 bp ...
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bioRxiv - Bioengineering 2024Quote: ... Deaminated DNA products were treated with proteinase K (NEB), followed by Endonuclease V (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... PCR products were digested using DpnI (NEB, Ipswich, MA) and purified using the MicroElute Cycle Pure kit (Omega ...
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bioRxiv - Biophysics 2024Quote: ... Nucleic acid was treated with DNase (NEB), purified using RNA SPRI beads ...
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bioRxiv - Developmental Biology 2021Quote: ... and eluted in 16.5 μl of Elution Buffer (10 mM Tris-HCl pH 8.5) followed by A-tailing reaction in 20 μl of dA-Tailing reaction buffer (NEB) with 2.5 U of Klenow fragment exo-(NEB ...
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bioRxiv - Genomics 2021Quote: ... All gDNA from day 2 and 10 μg gDNA corresponding to 3 million haploid cells from day 8 was amplified in PCR reactions with maximum of 2.5 μg of DNA per reaction using NEBNext High Fidelity master mix (New England Biolabs) and target-specific primers with Illumina adaptor flanks (Supplementary Tables 1 ...
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bioRxiv - Developmental Biology 2020Quote: ... and eluted in 16.5 μl of Elution Buffer (10 mM Tris-HCl pH 8.5) followed by A-tailing reaction in 20 μl of dA-Tailing reaction buffer (NEB) with 2.5 U of Klenow fragment exo- (NEB ...
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bioRxiv - Immunology 2021Quote: All PCR reactions were carried out in 25 μL total reaction volumes using 2X Master Mix (New England Biolabs), oligonucleotide primers synthesized by Eurofins (Ebersberg ...
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bioRxiv - Developmental Biology 2022Quote: ... and eluted in 16.5 μl of Elution Buffer (10 mM Tris-HCl pH 8.5) followed by A-tailing reaction in 20 μl of dA-Tailing reaction buffer (NEB) with 2.5 U of Klenow fragment exo-(NEB ...
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bioRxiv - Synthetic Biology 2024Quote: ... The PCR reaction was set up with 1 µL of the supernatant in a 12.5 µL reaction with Taq polymerase (NEB), according to manufacturer’s instruction.
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bioRxiv - Developmental Biology 2024Quote: A Primer Exchange Reaction concatemerization reaction (1X PBS; 10 mM MgSO4; dNTP, 0.6 mM of A, C, and T, NEB-N0446S ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR reactions contained 2 μl cDNA in 50 μl PCR reaction with Phusion Hot Start Flex (New England Biolabs). Reaction conditions ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified RNA from each phosphatase or control reaction was then used to set up two reactions 20 μL reactions containing 1 μg of treated RNA in NEBuffer 3.1 (New England Biolabs) with or without the addition of 1 μL (1U ...
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bioRxiv - Genomics 2024Quote: ... We performed PCR reactions using 1 ng of the pooled gRNA library plasmids per reaction with Q5 polymerase (NEB). The correct size band from the PCR product was then gel-purified from a 2% E-gel EX (Life Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... R1/R2 purified fragments were split in 12× 25 μl-reaction PCR reactions with Q5 High-Fidelity 2X Master Mix (New England Biolabs), and NEBNext® Multiplex Oligos for Illumina® ...
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bioRxiv - Microbiology 2021Quote: ... Sequence-independent PCR amplification was conducted with 5 μl of purified dsDNA obtained after Klenow reaction in 50 μl of final reaction which contained 1x Q5 High-Fidelity Master Mix (NEB), 2.5 μl of 10 μM primer K (5’-GACCATCTAGCGACCTCCAC-3’ ...
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bioRxiv - Synthetic Biology 2020Quote: ... Transcriptional units were amplified directly from the golden gate mixture using 1 µL of reaction mixture as template in 50 µL Q5 Hot-Start Master Mix reactions (NEB). PCR reactions were performed according to manufacturer’s instructions and cleaned up using clean and concentrate kits (D4004-Zymo Research) ...
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bioRxiv - Genomics 2021Quote: ... Methylation reactions were performed in a 100uL reaction containing 1X CutSmart Buffer and 1mM S-adenosyl-methionine (SAM, New England Biolabs) and incubated at 37°C for 30 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... We performed eight 100 µl PCR reactions per sample (4 µg DNA per reaction) using Q5 High-Fidelity 2x Master Mix (New England Biolabs)28 to maximize library sequencing quality ...
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bioRxiv - Genomics 2021Quote: ... The reactions were combined and 2.5 μL of the assembly reaction or a control reaction without amplicon were used to transform NEB5-alpha cells (New England Biolabs) to measure background assembly ...
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bioRxiv - Microbiology 2020Quote: ... the V1-V3 region of 16S rRNA genes was amplified from 2 ng of DNA in 50 μl reactions containing the following components: 1x Standard Taq Reaction Buffer (NEB), 3 mM MgCl2 (NEB) ...
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bioRxiv - Molecular Biology 2020Quote: ... End-repair reaction was cleaned using 1.8X Agencourt AMPure XP beads and eluted in 15 µl of EB that was used for A-tailing reaction in 30 µl of NEBNext dA-Tailing reaction buffer (NEB) with 7.5 units of Klenow fragment exo- (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... End-repair reaction was cleaned using 2X Agencourt AMPure XP beads and eluted in 16.5 μl of EB that was used for A-tailing reaction in 20 μl of NEBNext dA-Tailing reaction buffer (NEB) with 7.5 U of Klenow fragment exo-(NEB ...
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bioRxiv - Genomics 2020Quote: ... Reactions were performed in a 200uL reaction with 1X CutSmart Buffer and 1mM S-adenosyl-methionine (SAM, New England Biolabs) and incubated with 2.5uL enzyme at 37°C for 30 minutes ...
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bioRxiv - Genomics 2020Quote: ... Methylation reactions were performed in a 100 uL reaction with Methylation Reaction buffer (1X CutSmart Buffer,1 mM S-adenosyl-methionine (SAM, New England Biolabs)) and incubated with 2.5 uL EcoGII at 37°C for 30 minutes ...
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bioRxiv - Systems Biology 2020Quote: ... The full volume of each fill-in reaction was put into a dilute blunt-end ligation reaction (640 U T4 DNA Ligase, NEB) and allowed to incubate overnight at 15° C ...
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bioRxiv - Molecular Biology 2024Quote: ... Each methylation reaction was thoroughly mixed on ice using 100uL of cell methylation buffer (10% volume 10x M.CviPI reaction buffer (NEB, B0227S), 300 mM sucrose ...
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bioRxiv - Biochemistry 2024Quote: ... and tRNAGln with 5 nt-long 5’ leader and same 24 nt-long 3’ trailer as tRNATyr (5–tRNAGln–24) – were transcribed in reactions containing 1x T7 RNA polymerase reaction buffer (NEB), 0.001% (w/v ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR reaction and digested plasmid were gel purified and used to perform a Gibson Assembly reaction (Cat. #E2611S, New England Biolabs) followed by bacterial transformation according to the manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: PCR reactions were performed by 30μL reactions using 15μL of Phusion® High-Fidelity PCR Master Mix (New England Biolabs, USA), 0.2 μM of primers
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bioRxiv - Genomics 2023Quote: ... All PCR reactions were performed in 50 μL reactions using Q5 High-Fidelity 2X Master Mix (New England Biolabs, M0492L). Primers for genomic DNA amplification are included in Table S2 ...
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bioRxiv - Bioengineering 2023Quote: ... genomic loci were amplified in a first PCR reaction (PCR-1) reaction from approximately 100 ng of gDNA using Q5 High-fidelity DNA Polymerase (NEB) and the primers listed in Sup ...
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bioRxiv - Molecular Biology 2024Quote: ... In vitro RNA transcription was performed in 50 µl reactions: 5 µl RNAPol Reaction Buffer (New England BioLabs, Ipswich, MA), 2 µl T7 RNA Polymerase (New England BioLabs ...
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bioRxiv - Cancer Biology 2024Quote: ... perform 25-30 separate 100 µL reactions with 6-8 µg genomic DNA in each reaction using Q5 High-Fidelity DNA Polymerase (New England Biolabs) for around 18-20 cycles and then combine the resulting amplicons ...
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bioRxiv - Genetics 2024Quote: ... Golden Gate Assembly reaction was carried out in a 100ul reaction containing 2.5U Esp3I (Thermo ER0452) and 1000U T4 DNA Ligase in T4 DNA Ligase reaction buffer (NEB M0202L). The reaction mix was incubated for 31 cycles alternating between 37deg ...
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The ATP-dependent protease ClpYQ degrades cell division proteins DivIVA and Mbl in Bacillus subtilisbioRxiv - Biochemistry 2024Quote: ... Reaction mixtures were prepared with a 25 μL final volume and contained 1 X Φ29 DNA polymerase reaction buffer (NEB), 0.1 mg/mL recombinant albumin (NEB) ...
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bioRxiv - Biophysics 2021Quote: ... the reaction was treated with DNAseI (NEB M0303L) according to the manufacturer’s instructions and purified with 2.5 M LiCl precipitation ...
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bioRxiv - Evolutionary Biology 2020Quote: ... six parallel PCR reactions (Q5 DNA polymerase, NEB) were set up with 30 µl of sample ...
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bioRxiv - Microbiology 2021Quote: ... The reaction was then digested with DpnI (NEB), to digest methylated parental DNA ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 1X T4 RNA Ligase Reaction Buffer (NEB B0216L), and 20 uM pre-adenylated linkers ...
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bioRxiv - Genomics 2020Quote: ... we transformed the reaction into electrocompetent cells (NEB), and obtained 1.44 million unique transformants ...
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bioRxiv - Molecular Biology 2020Quote: ... x1 Exonuclease I Reaction Buffer (NEB, Cat #B0293), x1 CutSmart buffer (NEB ...
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bioRxiv - Genomics 2020Quote: 10X ThermoPol Reaction Buffer (NEB, cat. no. B9004S)
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bioRxiv - Systems Biology 2020Quote: ... 20 μL T7 DNA ligase reaction buffer (NEB), and 2 μL nuclease-free water then incubating at 37°C overnight ...
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bioRxiv - Biochemistry 2020Quote: ... Reagents for the KLD reactions came from NEB: T4 PNK (M0236S) ...
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bioRxiv - Bioengineering 2020Quote: ... a BsaI-golden gate reaction (New England Biolabs) was setup as described by NEB to fuse these ydgL and gudP amplicons ...
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bioRxiv - Biochemistry 2020Quote: ... using a Gibson assembly reaction (New England Biolabs). The resulting expression vectors pET22b-XXX-trans-mCherry (XXX stands for the respective protein ...
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bioRxiv - Neuroscience 2020Quote: ... 2.5 µl 10X reaction buffer (New England Biolabs), nuclease-free water to fill up to 25 µl total volume ...