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Citations for New England Biolabs :
451 - 500 of 4002 citations for Hexadecanoic acid reaction products with tetraethylenepentamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The HiFi products were transformed into NEB10β competent cells (NEB) and plated on LB agar containing 100 μg/mL ampicillin.
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bioRxiv - Microbiology 2023Quote: ... The product was restricted with enzymes BamH1-HF (NEB, R3136) and HindIII-HF (NEB ...
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bioRxiv - Immunology 2023Quote: ... PCR products and 100 bp DNA marker (New England Biolabs) were loaded on a 2% agarose gel in Tris-Acetate-EDTA buffer ...
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bioRxiv - Neuroscience 2023Quote: ... PCR products were digested with Aat II (New England Biolabs) for 4 h at 37° C ...
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bioRxiv - Genomics 2023Quote: ... The recombination products were then electroporated into electrocompetent cells (NEB 10-beta ...
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bioRxiv - Genetics 2023Quote: ... Each product was amplified using LongAmp Taq polymerase (NEB #M0323L). To avoid non-specific hybridization of the Lakl0C-left and Lakl0C-right probes to the rDNA locus ...
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bioRxiv - Microbiology 2023Quote: ... raw PCR product was mixed with T4 DNA ligase (NEB), DpnI (NEB) ...
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bioRxiv - Genomics 2023Quote: ... The PCR product is digested by the enzyme DPN1 (NEB) in order to remove the template plasmid and cloned in TOPO10 E ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were digested with 1 μL DpnI (NEB #R0176L) for 1 hour and purified by agarose gel extraction (MinElute Gel Extraction Kit ...
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bioRxiv - Microbiology 2023Quote: ... The amplified products were digested with DpnI (New England Biolabs) to remove the parental plasmid before transformation into DH5-alpha E ...
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bioRxiv - Bioengineering 2023Quote: ... Digested products were then ligated with T4 DNA Ligase (NEB).
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bioRxiv - Neuroscience 2023Quote: ... PCR products were purified with a DNA purification kit (NEB) and were then phosphorylated using T4 polynucleotide kinase (NEB ...
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bioRxiv - Developmental Biology 2023Quote: ... The PCR product was subjected to T4 PNK (NEB M0201S) and T4 DNA ligase (NEB M0202S ...
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bioRxiv - Microbiology 2023Quote: ... The PCR products were treated using DpnI (New England Biolabs) and purified using the Wizard SV Gel system and the PCR Clean-Up System (Promega).
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bioRxiv - Genetics 2024Quote: ... The products were treated with exonuclease I (New England Biolabs) and rapid alkaline phosphatase (Roche Diagnostics ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product was treated with DpnI (New England Biolabs). All plasmids were transformed into E ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified PCR products were digested with NotI (New England Biolabs) and ligated into pMON721 linearized with NotI to generate the SEGS-1 G ...
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bioRxiv - Genomics 2024Quote: ... The PCR products were treated with Exonuclease 1 (NEB, M0293S) to digest single-stranded products ...
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bioRxiv - Systems Biology 2024Quote: ... The PCR products were digested with DpnI (New England Biolabs) at 37°C for 1 hour to remove the original methylated template ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR products were digested with Dpn1 (NEB, Ipswich, MA, USA) to destroy the original template and were purified using the QIAquick Gel Extraction Kit (Qiagen ...
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bioRxiv - Plant Biology 2024Quote: ... DpnI-digested product was ligated using T4 DNA ligase (NEB) for 2 h at room temperature and transformed into NEB 5-alpha competent E ...
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bioRxiv - Immunology 2024Quote: ... while VL PCR products were digested with MluI (NEB, USA) and NotI (NEB ...
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bioRxiv - Microbiology 2024Quote: ... 5 μl inorganic pyrophosphate (New England Biolabs product number M2403), and ultrapure water (as needed ...
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bioRxiv - Microbiology 2024Quote: The product was then transformed into DH5-α cells (NEB). After plating onto LB-amp plates ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product was digested with EcoRI and SalI (NEB) and ligated (NEB T4 DNA ligase ...
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bioRxiv - Cancer Biology 2024Quote: ... the product was digested with the XmnI enzyme (NEB, R0194L) at 37°C for 3 hours ...
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bioRxiv - Immunology 2024Quote: ... The transcription products were then purified (New England Biolabs, T2050L) and the cap1 structure was added for efficient translation and evading the cellular innate immune response (New England Biolabs ...
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bioRxiv - Biochemistry 2024Quote: ... After treating the PCR products with DpnI (New England Biolabs) during 1 h at 37°C ...
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bioRxiv - Genetics 2024Quote: ... the 205 bp product was digested with ApoI (NEB, R0566) and incubated for 1 h at 50°C to generate 205 bp (wild-type ...
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bioRxiv - Genetics 2024Quote: ... the 116 bp product was digested with BccI (NEB, R0704) for 1 h at 37°C to generate 83 bp (wild-type ...
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RNA Binding of GAPDH Controls Transcript Stability and Protein Translation in Acute Myeloid LeukemiabioRxiv - Cancer Biology 2024Quote: ... End repair products were generated using T4 DNA polymerase (NEB), DNA polymerase I (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... The PCR products were treated with T4 PNK (NEB, M0201) for 2 hours at 37°C and subsequently purified using the QIAquick PCR Purification Kit (Qiagen ...
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bioRxiv - Biochemistry 2024Quote: ... then treating the PCR product with KLD enzyme mix (NEB). The treated PCR product was transformed into E ...
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bioRxiv - Genetics 2024Quote: ... All products were purified with Monarch DNA Cleanup Columns (NEB). T2A sequence was ordered as a gBlock from IDT ...
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bioRxiv - Cell Biology 2024Quote: ... The PCR product was digested with BamHI-HF® (NEB) and EcoRI (NEB ...
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bioRxiv - Developmental Biology 2024Quote: ... Enzyme digestion of the PCR product by BceAI (R0623L, NEB) had been used for the Gle1 KI mice to distinguish the carriers of the insertion ...
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bioRxiv - Molecular Biology 2022Quote: ... Beads were washed as described above and either directly resuspended in Proteinase K reaction or in 20 μl of RecJ adapter removal reaction (1X NEB Buffer 2 (NEB, #B7002S), 25U 5’ Deadenylase (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... 46 separate 60 μl PCR reactions were performed with 1 μg gDNA in each reaction using Q5® High-Fidelity DNA Polymerase (New England Biolabs). For the zeocin treated sample the total amount of genomic DNA harvested was used ...
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bioRxiv - Microbiology 2024Quote: ... RT-qPCR reactions were carried out in 10 μL reactions using the Luna® One-Step Universal RT-qPCR kit (NEB) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... Every marker was amplified in 20 μl of a reaction mix containing a 1X Standart Taq Reaction Buffer (M0273E, New England BioLabs Inc.), 0.4 μl of 10 mM dNTP ...
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bioRxiv - Molecular Biology 2024Quote: ... One microliter of the LEAP reaction then was amplified in a 25 μL PCR reaction using Q5 Hot Start High-Fidelity 2X Master Mix (NEB, M0494S) with primers specific for sequences within the SV40 promoter of the neoTet and mneoI reporter cassettes (NeoSV40_fwd ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2 μL of the diluted cDNA reaction was used in PCR reactions (25 μL) using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB, M0494S) with NeoSV40_fwd and 3′LEAPOuter primers ...
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bioRxiv - Immunology 2024Quote: ... the synthesis of biotin labeled probes was conducted by PCR in a 50 µl reaction volume with one Taq® standard reaction buffer (Biolabs), 0.2 mM dNTPs where dCTP ...
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bioRxiv - Synthetic Biology 2024Quote: ... Lentivirally integrated library constructs were amplified by PCR in 100ul reactions (5µg gDNA/reaction) using NEBNext Ultra II Q5 Master Mix (NEB Cat. M0544) from genomic DNA using dual-barcoded primers and the following PCR protocol ...
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bioRxiv - Biophysics 2022Quote: RE digestion reactions are prepared by adding 1/10th of the final reaction volume of 10x reaction buffer supplied by the RE manufacturer (typically NEB CutSmart buffer) and 0.1% Tween-20 to the DNA solution (diluted to the desired concentration in nanopure DI water) ...
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bioRxiv - Genomics 2024Quote: ... PCR reactions were performed with 5μL each of 5X Q5 Reaction Buffer and 5X Q5 High GC Enhancer (NEB, catalog no. B9027S), 0.5μL 10 mM dNTPs ...
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bioRxiv - Cell Biology 2020Quote: ... Transcription reactions were treated with RNase-free DNaseI (NEB) to digest the DNA template at 37°C for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: ... and OneTaq Standard Reaction Buffer (New England Biolabs, M0481). Amplicons were cloned into a pGEM-T Easy plasmid (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... in 1X T4 DNA ligase reaction buffer (NEB #B0202) for at least 2 hrs at room temperature with slow rotation on an orbital shaker ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μl 10X RtcB reaction buffer (New England Biolabs), 2 μl 1mM GTP ...