Labshake search
Citations for New England Biolabs :
301 - 350 of 1434 citations for GA binding protein alpha chain GABPA Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2024Quote: ... Donor and destination plasmids were combined in a single digestion-ligation reaction to generate an expression plasmid encoding the heavy and light chains with BsaI-HF (NEB) and T4 ligase (NEB) ...
-
bioRxiv - Plant Biology 2024Quote: ... The ITS region was amplified by polymerase-chain reaction (PCR) using the Phusion High-Fidelity DNA Polymerase (M0530L, New England Biolabs) and the following primers (as reported in (Jaramillo et al ...
-
bioRxiv - Genetics 2024Quote: ... 128 μL of sample was amplified in 400 μL polymerase chain reactions (PCRs) across 8 PCR tubes using Q5 High-Fidelity DNA Polymerase (NEB). Primers contained partial Illumina adapters ...
-
bioRxiv - Molecular Biology 2020Quote: ... polyadenylated RNA was purified by two rounds of binding to Oligo (dT)25 magnetic beads (NEB) and mRNA was fragmented with RNA fragmentation solution (Ambion ...
-
bioRxiv - Microbiology 2021Quote: ... SMU_313 with mutated SmsR4-binding site was constructed using the Q5 Mutagenesis Kit (New England Biolabs) and mutations were confirmed through Sanger sequencing.
-
bioRxiv - Developmental Biology 2020Quote: ... followed by mutation of the MAO binding site using the Q5 site directed mutagenesis kit (NEB) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: Antigen binding fragment (Fab) was generated by incubating IgG with LysC (New England Biolabs, Cat# P8109S) at a ratio of 1 μg LysC per 10mg IgG at 37°C for 18hrs ...
-
bioRxiv - Immunology 2020Quote: ... Antigen binding fragment (Fab) was generated by incubating IgG with LysC (New England Biolabs, Cat# P8109S) at a ratio of 1μg LysC per 10mg IgG at 37°C for 18hrs ...
-
bioRxiv - Bioengineering 2023Quote: ... the AsCas12a-crRNA complexes were combined with 1 µL of binding buffer (New England BioLabs, #B6002S), 4.5 µL of biotinylated quencher probes ...
-
bioRxiv - Genomics 2022Quote: ... a second bead binding was performed followed by a 5’ de-capping using RppH (NEB M0356S). The 5’ end of the transcript was phosphorylated using PNK (NEB M0201L ...
-
bioRxiv - Genomics 2021Quote: ... The reactions were combined and 2.5 μL of the assembly reaction or a control reaction without amplicon were used to transform NEB5-alpha cells (New England Biolabs) to measure background assembly ...
-
bioRxiv - Biochemistry 2022Quote: ... The following cloning strains were used: NEB Stable (lentiviral and piggybac vectors) and NEB 5-alpha (all other plasmids) (New England Biolabs). For cloning of base editor constructs ...
-
bioRxiv - Microbiology 2020Quote: ... Plasmids carrying the dcas9/lacI cassette were recovered and propagated in the NEB 5-alpha F’ Iq strain (New England Biolabs). Cloning and/or propagation of plasmids containing the dcas9/lacI cassette in host E ...
-
bioRxiv - Molecular Biology 2022Quote: ... following the manufacture’s protocol. Plasmids were transformed in DH5-alpha or DH10-beta chemo-competent Escherichia coli (E. coli) cells (New England Biolabs). Transformed bacteria were grown in LB medium supplemented with 50 μg/mL kanamycin or 100 μg/mL carbenicillin ...
-
bioRxiv - Synthetic Biology 2023Quote: ... 2 μl of the assembly reaction was transformed into 5 μl chemically competent cells (E. coli NEB 5-alpha, New England Biolabs). Circuit constructs were analyzed by PCR ...
-
bioRxiv - Cell Biology 2021Quote: ... 5 µg antibody (Table. S3) and 50 µl protein A magnetic beads (New England Biolabs, Cat. No. S1425S) were added to the supernatant and incubate overnight at 4 degree Celsius ...
-
bioRxiv - Neuroscience 2020Quote: Overlapping fragments of the unstable NaV1.1 cassette-3 were amplified in polymerase chain reactions (PCR) using Q5® Hot Start High Fidelity 2x Master mix (New England Biolabs) and the primer pairs listed in Table S1 ...
-
bioRxiv - Biochemistry 2020Quote: ... Novel mutants of Ecm2 were generated using inverse polymerase chain reaction (PCR) with Phusion DNA polymerase (New England Biolabs; Ipswich, MA). All plasmids were confirmed by sequencing.
-
bioRxiv - Microbiology 2021Quote: ... Zip codes were then amplified by polymerase chain reaction (PCR) from 200 ng of DNA template using Phusion ® High-Fidelity (HF) DNA Polymerase (New England Biolabs) in HF Buffer ...
-
bioRxiv - Cell Biology 2021Quote: ... neon-Green and mScarlet, these fragments were amplified by Polymerase Chain Reaction using Phusion® High-Fidelity DNA Polymerase (M0530L, New England Biolabs (NEB)) using 35 cycles with an annealing temperature of 60 °C (see table 2 for list of primers ...
-
bioRxiv - Bioengineering 2022Quote: ... Genes encoding individual components of biosensors were amplified by polymerase chain reaction (PCR) using Q5® High-Fidelity DNA Polymerase (New England Biolabs). Site-directed mutagenesis was performed by QuikChange lightning mutagenesis kit (Agilent ...
-
bioRxiv - Synthetic Biology 2020Quote: ... The genes of interest were amplified by polymerase chain reaction (PCR) using Q5 high fidelity DNA polymerase (New England BioLabs, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Synthetic Biology 2021Quote: minD,minE and FtsA genes were amplified by standard polymerase chain reaction (PCR) amplified using Phusion High-Fidelity DNA polymerase (New England Biolabs, USA) as previously reported24,30 ...
-
Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: sgDNA template was generated using gene-specific oligonucleotides and constant oligonucleotide by polymerase chain reaction for 30 cycles using Q5 high fidelity polymerase (NEB, USA). PCR product was further purified using Magbio Hiprep PCR cleanup system (Magbiogenomics ...
-
bioRxiv - Immunology 2020Quote: ... according to the manufacturer’s instructions.29 RNA reverse transcription and first strand cDNA synthesis were carried out using chain-specific reverse primers30 with ProtoScript® II First Strand cDNA Synthesis Kit (NEB). The resulting cDNA was used as template for preparing the sequencing library using 5’ multiplex PCR as previously described.31 The PCR products corresponding to the library amplification were purified on BluePippin with size cutoff around 500 bp ...
-
bioRxiv - Microbiology 2023Quote: RNA was used as template to detect and quantify viral genomes by duplex reverse transcriptase (RT) quantitative polymerase chain reaction (RT-qPCR) using a Luna Universal Probe one-step RT-qPCR kit (New England Biolabs, E3006E). SARS-CoV-2-specific RNAs were detected by targeting the ORF1ab gene using the following set of primers and probes ...
-
bioRxiv - Biochemistry 2023Quote: ... the MsbA gene (from Escherichia coli genomic DNA) was amplified by polymerase chain reaction (PCR) using Q5 High-Fidelity DNA Polymerase (New England Biolabs, NEB) and subcloned into a modified pCDF-1b plasmid (Novagen ...
-
bioRxiv - Neuroscience 2023Quote: ... as wildtype or T205A variant were amplified by polymerase chain reaction (PCR) using Q5 High-Fidelity master mix (M0492, New England Biolabs (NEB)) from previous plasmid constructs 16 ...
-
bioRxiv - Neuroscience 2023Quote: ... Mice were genotyped by polymerase chain reaction (PCR) using isopropanol-precipitated genomic DNA as template and OneTaq DNA polymerase (New England Biolabs, (NEB), #M0480 ...
-
bioRxiv - Biochemistry 2022Quote: The P562del and Exo+THR mutants were generated on the pET23-P2-D12A-THR (Table S1) background through inverse Polymerase Chain Reactions (iPCR) using Q5® High-Fidelity DNA Polymerase (NEB) following the manufacturer’s instructions in 25 μl reactions with primers p2_thumb_loop_R/DEL1 (Table S2 ...
-
bioRxiv - Immunology 2024Quote: ... we performed an A-tailing of the PCR products of the YTS191-light chain cDNA by adding 5 µl of 10X ThermoPol Buffer (NEB, B9004), 10 µl of 10 mM ATP ...
-
bioRxiv - Cancer Biology 2024Quote: The iDAb LMO2-E3 ligases fusions were generated by polymerase chain reaction (PCR) using Phusion® High-Fidelity PCR kit (NEB). pEF-VH576-L10-FLAG-CRBN-mb was used as a template to generate VH576-L10-FLAG-CRBN ...
-
bioRxiv - Molecular Biology 2021Quote: ... The binding assay was performed by incubating these radiolabelled DNA fragments with RNA polymerase holoenzyme (NEB #MO551S) for 5min at 25 °C followed by loading in 4% polyacrylamide gel in 1X TBE buffer ...
-
bioRxiv - Cell Biology 2022Quote: ... For CAK binding assays CDK2 was dephosphorylated by washing beads once in 1x λ-phosphatase buffer (NEB) supplied with 1 mM MnCl2 (NEB ...
-
bioRxiv - Systems Biology 2020Quote: ... that had been blocked and equilibrated into the binding buffer supplemented with 0.1% Tween 20 and 0.1μg/μl of BSA (Molecular Biology Grade, NEB). Protein-RNA complexes were then incubated with the magnetic beads on a shaker for further two hours ...
-
bioRxiv - Microbiology 2023Quote: ... fused to a maltose-binding tag using the pMAL™c5X-vector (New England Biolabs, Ipswich, US). Briefly ...
-
bioRxiv - Biochemistry 2024Quote: ... Genomic DNA flanking the CRISPR guide binding site was amplified by PCR using OneTaq (NEB, Hitchin, UK) according to the manufacturer’s instructions and using the primers indicated in table S1 ...
-
bioRxiv - Synthetic Biology 2023Quote: ... coli NEB 5-alpha (fhuA2∆(argF-lacZ)U169 phoA glnV44 ϕ 80∆(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17) (New England Biolabs) was used for the initial two-plasmid assay ...
-
bioRxiv - Cancer Biology 2024Quote: ... Purified DNA fragments were cloned into pcDNA3.1 vector and transformed into 5-alpha competent cells (#C2987H, New England Biolabs, Ipswich, MA) and 10 colonies were picked for each cell line ...
-
bioRxiv - Microbiology 2022Quote: ... Reverse-transcription polymerase chain reaction (RT-PCR) was performed using the Luna Universal Probe One-Step RT-qPCR kit (New England BioLabs; https://www.neb.ca). The 5 ‘ untranslated region (UTR ...
-
bioRxiv - Neuroscience 2022Quote: ... and VL in the vector pCSL3l/pCSL3k (light chain lambda/kappa)41 adapted for Golden Gate Assembly procedure with Esp3I restriction enzyme (New England Biolabs, Frankfurt, Germany). Expi293F cells were cultured at 37°C ...
-
bioRxiv - Genetics 2022Quote: ... The pBSMVγPDS plasmid and all plasmids expressing BSMV γ chain carrying sgRNAs were linearized with BssHII (New England BioLabs, catalog number R0199S). In vitro transcription was performed in 20 µL reaction volume using HiScribe™ T7 High Yield RNA Synthesis Kit (New England BioLabs ...
-
bioRxiv - Immunology 2020Quote: ... The heavy and light chain PCR products were cloned in frame with seamless cloning using the NEBuilder® HiFi DNA Assembly Master Mix (NEB, UK) after linearizing the vectors with KpnI (5’ ...
-
bioRxiv - Microbiology 2023Quote: ... and was subsequently inserted into a cloning vector pUCNDVH5 (28) by Phusion polymerase chain reaction (Finnzymes Phusion®, New England Biolabs®) (29) ...
-
bioRxiv - Cell Biology 2020Quote: ... The FER1 Ca2+-binding mutants in the C2D domain were generated by Q5 site directed mutagenesis kit (NEB) using primers 4833/4834 to change positions A1622 and A1634 to C resulting in Asp codon 542 and 545 changes to Ala.
-
bioRxiv - Microbiology 2021Quote: ... Mutations at the sgRNA binding sequence were introduced via mutagenesis using Q5 Site Mutagenesis following manufacturer’s protocol (NEB) using primers described in Supplementary Table 5.
-
bioRxiv - Biochemistry 2024Quote: The ATP binding mutants (APLFD11A, APLFR37A, APLFK95A) were generated using Q5® Site- Directed Mutagenesis Kit (NEB #E0554) according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2023Quote: ... MEMO1 was expressed as fusion with the chitin-binding domain and intein using vector pTYB12 (New England BioLabs) in E ...
-
bioRxiv - Biophysics 2024Quote: ... in the case of NINL-dynactin binding or 100nM dynein to 25 µL SNAP-Capture Magnetic Beads (NEB) in 2 mL Protein Lo Bind Tubes (Eppendorf ...
-
bioRxiv - Plant Biology 2021Quote: ... and attB2 DOG6 3’UTR-2-R (GGGG AC CAC TTT GTA CAA GAA AGC TGG GTC TCC TCC GTT AGG TTC CGT GA) and Phusion® High-Fidelity DNA Polymerase (New England Biolabs, Massachusetts, USA). This PCR product was cloned into cloned into pDONR221 ...