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Citations for New England Biolabs :
301 - 350 of 614 citations for Calcium Channel Voltage Dependent L Type Alpha 1C Subunit CACNA1C Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The relative abundance of oligomannose-type glycans was measured by digestion with Endoglycosidase H (per sample in 20 µl volume) (Endo H; New England Biolabs). A 6 µl aliquot of labelled glycans was combined with 1 µg endoH to a final volume of 20 µl ...
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bioRxiv - Synthetic Biology 2022Quote: ... Multilayer genetic circuit plasmids were constructed using PCR and Golden Gate assembly (GAA) using BsmBI Type IIS Enzyme (New England Biolabs), and sRNA plasmids using BsaI Type IIS Enzyme (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... Type I-F Cascade was co-expressed with 6xHis-MBP-TEV-TniQ and a type III-B crRNA in NiCo21 cells (NEB). Cells were then induced with 0.5 mM isopropyl at 18 °C for another 18–20 hours before harvesting ...
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bioRxiv - Microbiology 2023Quote: ... Variants of the Bil system were generated by amplification of the plasmid containing the wild-type Bil system12 using primers that contained the desired modification followed by treatment with KLD enzyme mix (NEB) according to the manufacturer’s instructions to obtain transformable plasmids ...
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bioRxiv - Developmental Biology 2023Quote: ... Isoseq library preparation was performed on 300 ng of RNA (RIN ≥ 9.3) of each cell type (isolated as described above) using NEBNext® Single Cell/Low Input cDNA Synthesis & Amplification (NEB), Iso-Seq Express Oligo Kit (PacBio ...
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bioRxiv - Synthetic Biology 2023Quote: ... were combined into a transcription unit plasmid in a Type IIS DNA assembly reaction using BsaI-HFv2 (New England Biolabs). The destination vector (p15A origin of replication ...
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bioRxiv - Biochemistry 2023Quote: The K164A mutant was made by site directed mutagenesis using wild type LhCE as template with the Q5 Site-Directed Mutagenesis Kit from NEB using following primers:
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bioRxiv - Biochemistry 2023Quote: ... and linearized by the appropriate Type IIS enzyme (BspQI or BsmBI) and purified by Monarch RNA Cleanup kit (New England Biolabs) prior to IVT reactions.
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bioRxiv - Molecular Biology 2023Quote: ... Proximal regions were then amplified from the wild-type construct to generate fragments with appropriate overhangs for assembly by NEBuilder HiFi DNA Assembly (New England Biolabs) into pKLM79 (cut with MluI and SpeI ...
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bioRxiv - Evolutionary Biology 2024Quote: DNA fragments for wild-type and mutant GART were ordered from IDT (see below) and PCR amplified with Phusion DNA polymerase (New England Biolabs) with FWD_adapter and REV_adapter primers from IDT (see below) ...
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bioRxiv - Developmental Biology 2024Quote: ... The genomic region around the miossa20 allele was amplified for 35 cycles using 57°C for annealing and identified by restriction enzyme digestion of the wild-type allele with BsaWI for 1 hour (New England BioLabs). The genomic region surrounding the spo11uc73allele was amplified for 35 cycles with an annealing temperature of 57°C and products were resolved without digestion48 ...
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bioRxiv - Genomics 2024Quote: ... and the wild-type (USH2A:c.7595-2144A) minigene plasmids were generated by Q5® High-Fidelity DNA Polymerase (New Englands Biolabs) amplification of the target USH2A region using gDNA of a human heterozygous USH2A:c.7595-2144A/G patient and subsequent cloning into the pSPL3 backbone vector by NEBuilder® HiFi DNA Assembly Cloning Kit (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2024Quote: All replicative plasmid backbones were constructed using the Golden Gate Assembly strategy with the type II restriction enzyme Esp3I (NEB) according to the manufacturer’s protocol (Table 1) ...
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bioRxiv - Plant Biology 2024Quote: ... Nucleotide changes leading to amino acid substitutions for Pic3 and Pic12 were first created in pENTR/D-TOPO (which contained wild-type Pic3 or Pic12) vectors by using the Q5 site-directed mutagenesis kit (New England Biolabs) with specific primers (Supplemental Table S4) ...
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bioRxiv - Biochemistry 2021Quote: ... Enzymatic deglycosylation of NTD was carried out by adding 2.5 L Endo Hf (NEB) per 20 μg of NTD and incubating for 24 hrs at 25 °C ...
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bioRxiv - Biochemistry 2020Quote: ... Deletion mutants of hnRNP L and SETD2 were constructed by PCR (Phusion polymerase, NEB) using full-length hnRNP L and SETD2 ...
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bioRxiv - Microbiology 2022Quote: ... purified using the Monarch RNA Cleanup Kit (T2050S/L, New England Biolabs, Ipswich, Massachusetts), reannealed by heating to 94°C and slowly cooling to room temperature over 50 min ...
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bioRxiv - Genomics 2022Quote: ... The NEBNext® Ultra DNA Library Prep kit for Illumina (cat# NEB #E7370S/L) was used to process the DNA samples ...
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bioRxiv - Cell Biology 2023Quote: ... RNAs were selected using the NEB Next Poly A+ Isolation Kit (NEB #E7490S/L). Poly A+ fractions were eluted ...
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bioRxiv - Synthetic Biology 2021Quote: ... This point mutation was corrected back to wild type by site-directed mutagenesis using Q5® Hot Start High-Fidelity DNA Polymerase (New England Biolabs) and primers ...
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bioRxiv - Cancer Biology 2022Quote: ... Assembly of custom lentiviral vectors expressing sgRNAs of choice was accomplished by Golden Gate cloning and type IIS restriction enzyme BsmBI (NEB, R0739), which cleaves outside its recognition sequence to create unique overhangs as previously reported (46) ...
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bioRxiv - Cell Biology 2019Quote: ... pREP81-FLAG vector and plasmids carrying wild type and mutated forms of rrp1+ and rrp2+ were constructed using Gibson Assembly Cloning Method/ Gibson Assembly® Cloning Kit (NEB). All primers used to amplify gene sequences by PCR are listed in Table S3 ...
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bioRxiv - Biophysics 2022Quote: ... MUT) by using a different set of type IIS enzymes (BbvI, New England Biolabs #R0173, and BfuAI, New England Biolabs #R0701). Importantly ...
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bioRxiv - Biochemistry 2023Quote: ... The mutant and wild-type target RNA were subsequently amplified using either the NEB Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit (NEB E3019), Luna Probe One-Step RT-qPCR 4X Mix with UDG (NEB M3019 ...
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bioRxiv - Developmental Biology 2024Quote: ... The genomic region around rbpms2aae30 was amplified for 35 cycles with an annealing temperature of 60°C and the wild-type allele was digested with HaeIII for 1 hour (New England Biolabs, R0108S)2 ...
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bioRxiv - Developmental Biology 2024Quote: ... The surrounding tsc2vu242 genomic region was amplified for 35 cycles with an annealing temperature of 57°C and the wild-type allele was digested with HpyCH4IV for 1 hour (New England Biolabs, R0169S). Undigested and digested products were resolved in a 3% Metaphor 1:1 (Lonza)/agarose gel (Invitrogen ...
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bioRxiv - Systems Biology 2023Quote: ... five reactions each with 250 fmol of the construct carrying the mutant fragment or the original wild-type pNES-EGFP-C1-PH-ARNO(I303E)x2 construct were digested with 20 U each of BamHI-HF (NEB #R3136S) and HindIII-HF (NEB #R3104S) ...
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bioRxiv - Microbiology 2024Quote: ... acidocaldarius DSM 639 wild type using the primers (Eurofins Genomics) listed in supplementary Table 4 employing the Q5 polymerase (NEB, USA) following the manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... antibody and anti-Gluc antibody (New England Biolabs, ref. E8023).
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bioRxiv - Developmental Biology 2023Quote: ... a MBP antibody (Anti-MBP Monoclonal Antibody, HRP conjugated, NEB), a GST antibody (GST Tag Monoclonal Antibody ...
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bioRxiv - Genomics 2022Quote: ... wild-type and mutant NEAT1 RNA fragments were transcribed in vitro using HiScribe™ T7 High Yield RNA Synthesis Kit (NEB, #E2040S) and labelled with Biotin using Biotin RNA Labelling Mix (Roche ...
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bioRxiv - Molecular Biology 2022Quote: The NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (NEB #E7370S/L) was used to prepare DNA sequencing libraries ...
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bioRxiv - Microbiology 2023Quote: ... NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7645S/L) was used for library preparation and libraries were sequenced on Illumina MiSeq or NextSeq 2000 platform and more than 1.25M read pairs of 150bp length were generated per sample i.e ...
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bioRxiv - Neuroscience 2019Quote: The sequence encoding wild-type PlexB (Joo et al., 2013) was amplified by Q5 hot-start high-fidelity DNA polymerase (New England Biolabs, Ipswich, MA, USA) and assembled into a pUAST-attB vector (Li et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein blots were incubated with primary antibodies overnight at 4°C using the above-mentioned antibodies and the Gaussia Rabbit Polyclonal Antibody (E8023S, New England BioLabs), the Influenza A NS1 Mouse Monoclonal Antibody (sc-130568 ...
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bioRxiv - Biochemistry 2021Quote: ... This was added to 5 ml (per 2 L of culture) amylose resin (New England Biolabs) equilibrated in lysis buffer and left on a tube roller shaker at 4 °C for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... The DNA was then digested using Dpn II (375 U DpnII (NEB, R0543 T/M/L) in 1× Dpn II buffer (NEB ...
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bioRxiv - Genetics 2023Quote: ... Libraries were prepared using the NEBNext Ultra II kit (New England Biolabs, cat. no. E7645S/L). Index primers set 1 and 2 from the NEBNext Multiplex Oligos for Illumina kit (New England Biolabs ...
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bioRxiv - Cell Biology 2019Quote: ... 0.25μl of each primer (from 25μM stock) and 4.5μl of Luna Universal Master Mix (New England Biolabs). The q-RT-PCR reactions were run on the QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Genomics 2019Quote: ... the 20 µl products of reverse transcription were directly mixed into reaction buffer (NEB, cat. E6111S/L) on ice to obtain dsDNA samples with the help of DNA polymerase I and T4 DNA ligase ...
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bioRxiv - Immunology 2021Quote: ... Libraries were indexed using NEBNext Multiplex Oligos for Illumina Unique Dual Index Primer Pairs (NEB #644OS/L). Library prep generated cDNA was quantified and analyzed using Agilent DNA chip and Qubit DNA dye ...
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bioRxiv - Neuroscience 2023Quote: ... Libraries were indexed using NEBNext Multiplex Oligos for Illumina Unique Dual Index Primer Pairs (NEB #644OS/L). Library prep generated cDNA was quantified and analyzed using Agilent DNA chip and Qubit DNA dye ...
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bioRxiv - Neuroscience 2023Quote: ... 10 µL phosphorylation mix (1µL 100mM sense oligo, 1µL 100mM anti-sense oligo, 0.5µL 25mM ATP (BioLabs #P0756S), 1µL 10X PNK T4 Buffer (BioLabs #B0201S) ...
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bioRxiv - Plant Biology 2021Quote: ... and anti-MBP antibodies (NEB), respectively.
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bioRxiv - Plant Biology 2021Quote: ... or anti-MBP antibodies (NEB).
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bioRxiv - Plant Biology 2021Quote: ... or anti-MBP antibody (NEB).
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bioRxiv - Plant Biology 2022Quote: ... The samples were pooled by row into 8-strip tubes and excess primers were digested with the addition of 4.6 µL exonuclease I mix (2.5 µL of 10X Exonuclease I Buffer, 2.1 µL Exonuclease I; New England Biolabs) then incubated at 37□ for 20 min ...
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bioRxiv - Immunology 2021Quote: ... 3.6 μL of exonuclease reaction solution (2.52 μL sterile nuclease-free water, 0.36 μL Exo reaction buffer, and 0.72 μL Exo-I, New England BioLabs) was then added to remove unincorporated primers (37°C for 30 min ...
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bioRxiv - Genomics 2021Quote: dATP was added to the 3’ ends by adding 9μL of A-tailing mix (5μL NEB buffer 2.1, 1μL of 1mM dATP, 5U Klenow exo (NEB M0212S)) to the 41μL of DNA sample from the previous step ...
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bioRxiv - Genomics 2021Quote: ... dATP was added to the 3’ ends by adding 9μL of A-tailing mix (5μL NEB buffer 2.1, 5μL of 1mM dATP, 3U Klenow exo (NEB M0212S)) to the 41μL of beads with bound DNA from the previous step ...