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Citations for New England Biolabs :
301 - 350 of 868 citations for 6 amino 7H benzo e perimidin 7 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... the corresponding single and multiple amino acid substitutions were introduced by QuikChange site-directed mutagenesis (NEB, Phusion High-Fidelity PCR Kit) followed by KLD (Kinase ...
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bioRxiv - Neuroscience 2020Quote: ... amino acid sequence identical to GenBank: BC002453.2) was amplified using Q5 High-Fidelity DNA Polymerase (New England Biolabs, Ipswich MA, USA) and inserted into the TOPO cloning site in pET151/D-TOPO E ...
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bioRxiv - Molecular Biology 2021Quote: ... or more amino acid substitutions in SecPH with Q5 PCR methodology (Q5®Site-Directed Mutagenesis Kit, New England Biolabs, E0554) or PCR site-directed mutagenesis strategy ...
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bioRxiv - Immunology 2021Quote: ... were generated by introducing the corresponding amino acid mutations (Extended Data Fig. 5) using the Q5® Site-Directed Mutagenesis Kit (NEB) and per manufacturer’s protocol.
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bioRxiv - Microbiology 2021Quote: ... The OprCAA mutant was produced by changing the key amino acids Cys143 and Met147 to alanine residues using the KLD Quickchange site-directed mutagenesis kit (New England Biolabs, UK) and specific primers containing both mutation sites (forward ...
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bioRxiv - Cell Biology 2019Quote: ... The cDNA coding region corresponding to RDGB(USR1-LNS2)Δ (amino acids 1-472) was subcloned into pUAST-attB by using the restriction enzymes NotI and XbaI (NEB). Similarly ...
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bioRxiv - Molecular Biology 2023Quote: ... peptide sequences specific to an aMino sdAb were generated using the Ph.D.™-C7C Phage Display Peptide Library Kit (New England BioLabs, E8120S), a combinatorial library consisting of randomized display peptides with a disulphide constrained loop (AC-XXXXXXX-CGGGS ...
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bioRxiv - Cell Biology 2023Quote: ... The C-terminal truncation of murine STING (to include only amino acids 1-339) was accomplished using NEB Q5 High-Fidelity 2X Master Mix (NEB M0492S) per the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... Gene-specific primers were used on cDNA (OneTaq One-Step RT-PCR, New England Biolabs) to evaluate aberrant transcript splicing via gel electrophoresis ...
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bioRxiv - Molecular Biology 2021Quote: ... Single step PCR was performed using a One-Taq™ PCR reaction kit (NEB, USA) with 30 amplification cycles and 2 ng of original circular-DNA extract as the DNA template ...
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Efficient Suppression of Endogenous CFTR Nonsense Mutations Using Anticodon Engineered Transfer RNAsbioRxiv - Molecular Biology 2021Quote: ... using the Luna Universal One-Step RT-qPCR Kit (New England BioLabs, #E3005L or #E3006E) according to manufacturer’s instructions and then analyzed with QuantStudio Design & Analysis Software v1.5.1 (Applied Biosystems ...
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bioRxiv - Microbiology 2019Quote: The purified aptamer pool was then amplified by PCR with One Taq DNA polymerase (NEB), according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2022Quote: ... These filled-in primers are then amplified by PCR with One-Taq DNA polymerase (NEB) and two 21-base primers (HTOP and HBOT ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were run using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) on a CFX384 (Bio-Rad).
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bioRxiv - Biophysics 2021Quote: ... We used the Luna Universal One-step RT-qPCR kit (E3005S, New England Biolabs, MA) to determine the relative abundance of target mRNA between two samples ...
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bioRxiv - Developmental Biology 2020Quote: ... which was injected into one-cell state embryos together with Cas9 protein (New England Biolabs). Mutant animals were identified by PCR using the primers ...
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bioRxiv - Immunology 2021Quote: ... and 60 ng were used for the One Taq RT PCR Kit (New England Biolabs) according to the instructions provided by the manufacturer for mRNA quantification and reverse transcription (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR was performed using Luna Universal One-Step RT-qPCR kit (NEB, Cat. # E3005E) following manufacturer’s instructions ...
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bioRxiv - Zoology 2020Quote: One microliter of cDNA was amplified by PCR using OneTaq DNA Polymerase (New England Biolabs) with with the following primers for the two putative SIFamide receptor ...
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bioRxiv - Genetics 2020Quote: ... One aliquot of the digested DNA was subjected to overnight RNase H (NEB cat#M0297) treatment at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... using Luna Universal Probe One-Step RT-qPCR Kit (New England BioLabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... and 16s rRNA transcripts by the Luna Universal One-Step RT-qPCR kit (NEB, E3005), according to the manufacturer’s instructions on an Applied Biosystems 7500 Fast Real-Time PCR System ...
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bioRxiv - Neuroscience 2022Quote: ... The RT-qPCR was performed using Luna One Step RT-qPCR mix with dUDG (NEB). Amplification using Luna One Step qPCR mix with UDG (NEB ...
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Circularization of rv0678 for genotypic bedaquiline resistance testing of Mycobacterium tuberculosisbioRxiv - Molecular Biology 2022Quote: One hundred nanograms of the amplicon was incubated with 10U of TelN Protelomerase (NEB, USA) according to the manufacturer’s instructions at 30°C for 30 minutes ...
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bioRxiv - Plant Biology 2023Quote: ... RT-qPCR was performed using a Luna ® Universal One-Step RT-qPCR kit (NEB) in a CFX96 qPCR thermocycler (BioRad) ...
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bioRxiv - Biochemistry 2023Quote: ... For assessment of HAC1spliced mRNA the Luna Universal Probe One-Step RT-qPCR Kit (NEB) was used with 2 µl of a 50 ng/ µl RNA sample ...
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bioRxiv - Cell Biology 2023Quote: ... RT-qPCR was performed using Luna® Universal One-Step RT-qPCR Kit (E3005S, NEB). 20μl reactions using 200nM primers and <100ng RNA were run on ABI StepOnePlus Real Time PCR system following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were run using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) on a CFX384 (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs, Ipswich, MA, USA) and a BIORAD CFX Opus 384 system ...
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bioRxiv - Biochemistry 2023Quote: ... 1 μg of one plasmid was digested overnight with 10 units PacI (New England Biolabs) in 20 μL at 37 °C ...
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bioRxiv - Plant Biology 2024Quote: ... and first strand cDNA was synthesized with One-Taq RT-PCR kit (New England Biolabs), according to manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2024Quote: ... The first one made use of Q5 mutagenesis following the manufacturer’s instructions (New England Biolabs), but due to the high GC-content of exon1 specific for the ZNRF3 long isoform ...
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bioRxiv - Genomics 2024Quote: ... a SYBR green I based qRT-PCR kit (Luna Universal One-Step NEB, Ipswich, USA) was used in PCR mixtures (20 µl ...
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bioRxiv - Microbiology 2024Quote: ... template genomic DNA was prepared by picking one colony into 50 μL ThermoPol buffer (NEB) with 0.5 μL thermolabile Proteinase K (NEB) ...
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bioRxiv - Genomics 2022Quote: ... 7 μl PEG 8000 (17.5% final) and 1 μl of T4 RNA ligase 2 KQ (NEB, M0373L) in 1X T4 RNA ligase buffer ...
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bioRxiv - Biophysics 2021Quote: ... and 3 samples from each replicate combined into 7 mL with 1 × T4 DNA Ligase Buffer (NEB), with 1% Triton X-100 ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA pellets were resuspended in 5 µL dephosphorylation reaction mix (7 mM Tris pH 8, 1x NEB T4 PNK buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... and elongation for 7 min at 72°C) using the Phusion High-Fidelity DNA Polymerase (NEB, #M0530S) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... and elongation for 7 min at 72°C) using the Phusion High-Fidelity DNA Polymerase (NEB, #M0530S) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... 1 µg of DNA was mixed with 7 µl NEBNext Ultra II end prep reaction buffer (NEB) and 3 µl Ultra II end prep enzyme mix (NEB) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Ni-NTA resin elute was immediately diluted with PBS containing 1 mM EDTA (PBS-E) and incubated with amylose resin (New England Biolabs) for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... 1μl of each lysate was used as a PCR template to amplify 600-800 bp fragments around the edited E-box using the Q5 High-Fidelity 2X Master Mix (NEB). The resulting PCR products were purified in 96-well plates using the AmpliClean DNA Cleanup Kit (Nimagen ...
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bioRxiv - Developmental Biology 2021Quote: ... They were then incubated overnight at 37 °C in Hybridization Buffer (10% Formamide, 10% 20x SSC, 400 µg/ml E. coli tRNA (New England Biolabs), 5% dextran sulfate ...
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bioRxiv - Microbiology 2021Quote: ... Capped mRNAs were again purified by LiCl4 precipitation followed by the addition of polyA tails using E coli Poly(A) polymerase (NEB) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: The recombinant constructs (pETDuet-1-TaPHB-1 and pETDuet-1-TaPHB-1-RUVBL1 were separately transformed into E. coli Lemo21(DE3) (NEB) chemical competent cells ...
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bioRxiv - Plant Biology 2021Quote: ... Constructs for protein expression in Escherichia coli (E. coli) were either inserted into a Gateway-cloning-compatible version of pMal-C2 (New England Biolabs, NEB) to create a fusion to the Maltose-binding protein (MBP ...
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bioRxiv - Biochemistry 2021Quote: ... 6His(TEV)nsp8 and nsp7L8(TEV)6His were expressed under the control of a T5-promoter in pQE30 vectors in Escherichia coli (E. coli) NEB Express C2523 cells (New England Biolabs) carrying the pRARE2LacI (Novagen ...
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bioRxiv - Microbiology 2021Quote: ... tuberculosis H37Rv (ATCC#25618) were procured through ATCC (USA). Escherichia coli (E. coli) strains DH5α and BL21 (DE3) were procured from NEB and were cultured in Luria–Bertani medium ...
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bioRxiv - Microbiology 2021Quote: ... Insertion of hACE2 into pLVX-IRES-puro was conducted by doubl e digestion of XhoI and XbaI (Fermantas) and ligation of T4 ligase (NEB) according to manufact urer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... following the manufacture’s protocol. Plasmids were transformed in DH5-alpha or DH10-beta chemo-competent Escherichia coli (E. coli) cells (New England Biolabs). Transformed bacteria were grown in LB medium supplemented with 50 μg/mL kanamycin or 100 μg/mL carbenicillin ...