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Citations for New England Biolabs :
551 - 600 of 868 citations for 6 amino 7H benzo e perimidin 7 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... 100ng of each entry and destination vector are assembled in one reaction mix containing 10U BsaI-HF-v2 (NEB), 200U T4 Ligase (NEB) ...
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bioRxiv - Synthetic Biology 2019Quote: ... PCR were performed using Q5 PCR master mix and One-Taq quick load master mix for colony PCR (NEB), primers were purchased from IDT (Louvain ...
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bioRxiv - Developmental Biology 2019Quote: ... single copy scGESTALT.2 F1 male transgenic adults were crossed to wildtype female adults and one-cell embryos were injected with 1.5 nl of Cas9 protein (NEB) and sgRNAs 1-8 in salt solution (8 µM Cas9 ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR were performed using Q5 PCR master mix and One-Taq quick load master mix for colony PCR (NEB), primers were purchased from IDT (Louvain ...
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bioRxiv - Synthetic Biology 2021Quote: ... USA). DNA amplification from a single colony (i.e. colony PCR) was performed with One Taq 2× Master Mix (NEB). Electrocompetent P ...
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bioRxiv - Microbiology 2019Quote: ... RT-qPCR was conducted in technical triplicate using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) according to the manufacturer’s instructions in 10 µl reaction volumes and reactions were run on a CFX Real-Time PCR Detection System (BioRad) ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was diluted with one volume of water and treated with 0.5 μL of 20 mg/mL proteinase K (New England Biolabs) at 50°C for 2 hours ...
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bioRxiv - Microbiology 2020Quote: ... THUNDERBIRD Probe qPCR Master Mix (Toyobo) for MERS-CoV and Luna Universal Probe One-Step RT-qPCR Kit (NEB) for SARS-CoV-2.
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bioRxiv - Cancer Biology 2020Quote: ... sgRNAs were amplified using a one-step barcoding PCR using NEBNext High Fidelity 2X PCR Master Mix (NEB, M0541L) and the following primers:
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bioRxiv - Synthetic Biology 2021Quote: ... Table S2.1) for hydrogel functionalisation were synthesised by mixing one volume of 40 mM BG-PEG-NH2 (NEB S9150S) or 40 mM chloroalkane-PEG-NH2 (Promega P6741 ...
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bioRxiv - Microbiology 2020Quote: ... RdRP_SARSr-F2 (GTGARATGGTCATGTGTGGCGG) and dRP_SARSr-R1(CARATGTTAAASACACTATTAGCATA) using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) and a CFX96 Real-Time System ...
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bioRxiv - Microbiology 2021Quote: ... Or reverse transcription and qRT-PCR were conducted in one step using 0.1 μg RNA as template with the Luna Universal Probe One-Step RT-qPCR kit (New England Biolabs) following manufacturer’s protocols ...
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bioRxiv - Biochemistry 2022Quote: ... The lysed cells were directly subjected to qRT-PCR using Luna Universal One-Step RT-qPCR Kit (NEB, #E3005L). The primer sets used in qRT-PCR to detect CI-MPR and CD-MPR mRNA are 5’-CATTCAGTGGGTGACTCTGTT-3’/5’-TGCTCTGGACTCTGTGATTTG-3’ and 5’-GCTCTAGTGAAGAGGCTGAAAC-3’/ 5’-GCACACCCTGAAGATGTAGATG-3’ ...
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR was performed using Luna® Universal Probe One-Step RT-qPCR kit (E3006L; New England Biolabs (NEB), Ipswich ...
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR was performed using Luna® Universal Probe One-Step RT-qPCR kit (E3006L; New England Biolabs (NEB), Ipswich ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Quantification of viral genomes was performed using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) with a primer/probeset binding in the orf1ab region (forward ...
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bioRxiv - Immunology 2022Quote: ... quantitative reverse transcription PCR (qRT-PCR) was conducted using Luna universal probe one-step RT-PCR kit (NEB, #E3006) on CFX96 Touch Real-Time Detection System on 96-well plates (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... P13-P19) (73) were carried out using Luna Universal One-Step RT-qPCR Kit (New England Biolabs; Frankfurt (Main), Germany ...
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bioRxiv - Genomics 2023Quote: The standard HiDEF-seq v2 protocol was followed with the exception of replacing Klenow fragment 3’→5’ exo-polymerase with one of the following: 9.6 U Bst large fragment (NEB), 9.6 U Bst 2.0 (NEB) ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription of RNA and quantitative PCR was performed using the Luna Universal One-step RT-qPCR Kit (NEB) using 1 µg of RNA per reaction ...
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bioRxiv - Synthetic Biology 2023Quote: ... and terminator) was assembled in one-pot Type IIS DNA assembly reaction using BsaI-HFv2 (New England Biolabs, NEB). Transcription units to assay the sensor output contained the PQS promoter expressing the gfp gene ...
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bioRxiv - Synthetic Biology 2023Quote: ... and terminator) was assembled in one-pot Type IIS DNA assembly reaction using BsaI-HFv2 (New England Biolabs, NEB). Transcription units to assay the sensor output contained the PQS promoter expressing the gfp gene ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by the addition of one µl of Luna Cell Ready Stop Solution 10X (New England Biolabs, Ipswich, MA). We extracted DNA (111 ...
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bioRxiv - Immunology 2023Quote: ... and the RT-qPCR master mix prepared using a Luna Universal One-Step RT-qPCR Kit (New England Biolabs), as per the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... Each 10 µL reaction contained Luna Probe One-Step RT-qPCR 4X Mix (New England Biolabs, Ipswich, MA, USA), primers (10 µM) ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA expression was determined using SYBR green Luna Universal One-step RT-PCR kit (New England Biolabs, Cat #E3005L) with a BioRad CFX96 thermocycler ...
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bioRxiv - Biochemistry 2023Quote: ... In order to remove the EndoHf from the HPAMPD the supernatant was brought to 100 mM Tris -HCl pH 7.5 and 100 mM NaCl and applied to a one ml amylose resin (NEB) column at a rate of about 1 drop per second at 25° C ...
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bioRxiv - Microbiology 2023Quote: ... This was followed by PCR using One Taq Quick-Load 2x Master Mix with Standard Buffer from BioLabs (UK).
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bioRxiv - Cancer Biology 2024Quote: ... One genotyping PCR sample contained 10 µL OneTaq® Quick-Load® 2X Master Mix (M0486L, New England Biolabs), 1 µL 10 µM forward primer ...
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bioRxiv - Neuroscience 2023Quote: ... One piece of brain tissue (∼300 mg) was placed in 3 ml of ice-cold nuclei extraction buffer (NEB) [0.32 M sucrose ...
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bioRxiv - Developmental Biology 2024Quote: ... and both reverse transcription and PCR were performed using the Luna One-Step Universal RT-qPCR kit (NEB, E3005S) and carried out on the Mic qPCR Machine (BioMolecular Systems ...
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bioRxiv - Genomics 2020Quote: ... 6 μL of DNA was subject to restriction digestion with McrBC (New England Biolabs, US) at 37°C for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... depleted with the NEBNext rRNA Depletion Kit (Human/Mouse/Rat, NEB, Figures 6 and S6), or not depleted (Figures 2B ...
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bioRxiv - Biochemistry 2019Quote: ... The cDNA was amplified for 6-16 cycles (primers below) with Phusion Polymerase (NEB, #E0553L) and gel-purified with ZymoClean Gel DNA Recovery Kit (Genesee Scientific ...
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bioRxiv - Biophysics 2020Quote: ... Digestion mix (84 μl water, 10 μl 10x NEB Buffer 3.1, 6 μl Nt.BspQ1 (NEB)) was prepared and heated to 50 oC for 5 minutes ...
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bioRxiv - Genomics 2019Quote: ... followed by the addition of 0.5 µl of 250 mM Random Primer 6 (S1230S, NEB) and 1 µl Deoxynucleotide Solution Mix (N0447 ...
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bioRxiv - Synthetic Biology 2021Quote: ... 6 were generated by PCR amplification (Phusion High-Fidelity PCR Kit, New England Biolabs #E0553) of an oligo that includes a T7 promoter ...
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bioRxiv - Microbiology 2021Quote: ... and 6 µg was digested with 2U/µg DNA of the restriction endonuclease Sau3AI (NEB). Ligation with 400 U of T4 DNA ligase (NEB ...
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bioRxiv - Genomics 2019Quote: ... The samples were further digested for 6 hr by 25 U of I-SceI (NEB). I-SceI was also heat inactivated ...
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bioRxiv - Biochemistry 2021Quote: C-terminally (His)6-tagged LukE was expressed in BL21 (DE3) Escherichia coli cells (NEB). Transformed cells were grown at 37 °C in Terrific broth supplemented with 100 μg/mL ampicillin to a density of OD600 = 0.6 ...
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bioRxiv - Biophysics 2020Quote: ... Digestion mix (84 μl water, 10 μl 10x NEB Buffer 3.1, 6 μl Nt.BspQ1 (NEB)) was prepared and heated to 50 oC for 5 minutes ...
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bioRxiv - Immunology 2022Quote: ... a 6-Helix construct was expressed recombinantly in BL21 (DE3) Escherichia coli (New England Biolabs). This construct was composed of three NHR and three CHR peptides with intervening glycine/serine linkers and a C-terminal hexahistidine purification tag ...
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bioRxiv - Molecular Biology 2023Quote: ... 6 μL 20mg/ml BSA and 5 μL 400U/μl T4 DNA Ligase (NEB, M0202) overnight at 16°C (tumbling ...
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bioRxiv - Genetics 2021Quote: ... the poly (A) product was broken into pieces at 94 °C for 5-7 min using the Magnesium RNA Fragmentation Module (NEB, MA, USA). The RNA fragments were then reverse-transcribed using the SuperScript™ II Reverse Transcriptase (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... The radioactive probes were prepared by end labelling 10 pmoles of DNA oligonucleotide complementary to the sfRNA (Supplementary table 7) with [γ-32P]-ATP (Perkin-Elmer, USA) using T4 polynucleotide kinase (NEB, USA). Unincorporated nucleotides were then removed by gel filtration on Illustra MicroSpin G-25 Columns (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... The PCR enrichment of adaptor-ligated DNA was conducted with 7 cycles and NEBNext® Multiplex Oligos for Illumnina® (NEB, USA) for single end barcoding ...
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bioRxiv - Microbiology 2020Quote: ... as previously described[7] Phage DNA libraries were prepared using NEBNext Ultra II FS library Prep and Kit for Illumina (New England Biolabs, Ipswich, USA). The sequencing was performed on the Illumina iSeq platform as part of a flowcell (2 x 151 ...
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bioRxiv - Genomics 2021Quote: ... 1000 ng samples of high quality total RNA (RIN≥ 7) was used for mRNA isolation using NEBNext Ploy(A) mRNA Magnetic Isolation module (New England Biolabs, catalog # E7490), followed by RNA library construction with NEBNext Ultra II Directional Lib Prep (New England Biolabs ...
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bioRxiv - Genomics 2020Quote: ... and once the solution is clear 48 uL of the elution was transferred to a 2mL Lo-Bind tube with End-Repair and A-tail buffer (7 uL) and enzymes (3 uL) premixed (NEBNext® Ultra™ II kit, New England Biolabs). The solution was then mixed by gentle tapping and transferred to a 0.2 mL PCR tube and incubated at 20 degrees C for 30 minutes and 65 degrees C for 30 minutes in a thermocycler ...
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bioRxiv - Immunology 2023Quote: ... PCR was performed with 2 ng of library input per reaction and 7 cycles of amplification using Q5 DNA polymerase (New England Biolabs, catalog # M0491S). Libraries were quantified using a Qubit 4 Fluorometer (Thermo Fisher Scientific ...