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Citations for New England Biolabs :
3301 - 3350 of 6023 citations for 7 Oxa 1 2 diazaspiro 4.4 non 1 en 6 one 4 methyl cis 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... containing 6,25µl of LongAmp Taq 2✕ Master Mix (NEB), 4,25µl of milliQ water ...
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bioRxiv - Genomics 2024Quote: ... 10 µl of 2% BSA (NEB, B9000S, 0.01% final) and 930 µl of nuclease-free water and supplemented with 1U/ml Protector RNase inhibitor (Millipore Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... 25 μL NEBNext HiFi 2 × PCR Master mix (NEB) was added ...
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bioRxiv - Genomics 2022Quote: ... 2 µL 400U/µL T4 DNA ligase (NEB, #M0202V), 10 µL 10mM ATP (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... BamHI (Site 2) or HindIII (Control; New England Biolabs) overnight at 37°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 2 µl of 10X ExoI buffer (NEB, B0293S). The annealing was performed in a thermocycler by gradually decreasing the temperature from 98 °C to 60 °C ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 2 mM MnCl2 (New England Biolabs, NEB). Control treated cells were incubated with a volume of 50% glycerol equivalent to the volume of RNase III added as well as 2 mM MnCl2 ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 2 mM MnCl2 (New England Biolabs, NEB). Control treated cells were incubated with a volume of 50% glycerol equivalent to the volume of RNase III added as well as 2 mM MnCl2 ...
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bioRxiv - Biochemistry 2023Quote: ... 2 uL dNTP Solution Mix (10 mM stock, NEB), 2 μl Taq DNA ligase (80 U ...
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bioRxiv - Plant Biology 2023Quote: ... 2 and T4 DNA ligase (both New England Biolabs).
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bioRxiv - Genomics 2023Quote: ... and digested with 2 units of β-agarase (NEB) per block (1 hr. ...
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bioRxiv - Biochemistry 2023Quote: ... SNAP-Cell TMR Star (2 mM in DMSO, NEB), and SNAP-Cell Block (2 mM in DMSO ...
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bioRxiv - Biochemistry 2023Quote: ... and SNAP-Cell Block (2 mM in DMSO, NEB) were prepared and stored as aliquots in the dark at –20 °C ...
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bioRxiv - Genomics 2023Quote: ... and digested with 2 units of β-agarase (NEB) (42°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 units of DNase I (New England Biolabs, M0303S) were added ...
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bioRxiv - Bioengineering 2023Quote: ... and mRNA Cap 2’-O-methyltransferase (NEB, catalog #M0366S). We then added a 3′-poly(A ...
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bioRxiv - Biochemistry 2023Quote: ... 2 µl PNGaseF (P0705L, Glycerol-free, New England Biolabs) was added and incubated for one hour at 57⁰C ...
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bioRxiv - Bioengineering 2023Quote: ... 2 µL of PNGase F (New England Biolabs, MA) was added ...
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bioRxiv - Genomics 2024Quote: ... 2 mg/mL temperature labile proteinase K (NEB, P8111S), and 12.5 mM MgCl2 for RNA fragmentation) ...
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bioRxiv - Biochemistry 2024Quote: ... using Q5® High-Fidelity 2× Master Mix (NEB) and 200 nM of each primer ...
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bioRxiv - Biochemistry 2024Quote: ... 2 µL 10× T4 RNA Ligase Reaction Buffer (NEB), 6 µL PEG8000 (50%) ...
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bioRxiv - Microbiology 2024Quote: ... 200 U T4 RNA ligase 2 truncated KQ (NEB), 1x T4 RNA ligase buffer (NEB) ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 2 µl of DNase I (2500U/mL, NEB) for each mL of B-PER reagent used ...
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bioRxiv - Cancer Biology 2024Quote: ... 25 µl NEBNext HiFi 2× PCR Master mix (NEB) was immediately added and mixed before proceeding to PCR cycles (58 °C for 5 min (gap filling) ...
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bioRxiv - Biophysics 2021Quote: ... 1 μL of this was then combined with 5 μL Q5 buffer (NEB, cat#M0491 S), 0.5 μL 10mM DNTP (Thermo Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... or a polyclonal rabbit antibody against cleaved casp-3 (1:100; New England Biolabs, Frankfurt, Germany), followed by a biotinylated goat anti-rabbit secondary antibody (Abcam ...
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bioRxiv - Cell Biology 2020Quote: The PAQR-2p∷PAQR-1∷GFP construct was generated with a Gibson assembly cloning kit (NEB) with the following three fragments ...
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bioRxiv - Genomics 2020Quote: ... 1 μL of 10 mM dATP and 15 units of Klenow 3’ → 5’ exo- (NEB M0212L) was added to blunted ends and incubated at 37°C for 30 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... and 5’ adaptor (rGrUrUrCrArGrArGrUrUrCrUrArCrArGrUrCrCr-GrArCrGrArUrCrNrNrNrCrGrArNrNrNrUrArCrNrNrN, N is for a random nucleotide) by T4 ligase 1(NEB) sequentially ...
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bioRxiv - Genomics 2022Quote: ... The electroporated bacteria was immediately split into ten 1 mL aliquots of outgrowth medium (NEB, B9035S) and incubated at 37°C for 1 hr then independently scaled up in 20 mL of LB supplemented with 100 μg/mL of carbenicillin (Teknova ...
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bioRxiv - Genomics 2020Quote: ... and barcoded with NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) (New England Biolabs). We sequenced prepared libraries on an Illumina MiSeq using MiSeq Reagent Kit v3 (2×300 cycles ...
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bioRxiv - Genomics 2020Quote: ... All ligations were supplemented with 1:40 RNAse inhibitor (ThermoFisher Ribolock or NEB Murine RNase Inhibitor) to prevent RNA degradation.
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bioRxiv - Genomics 2020Quote: ... All ligations were supplemented with 1:40 RNAse inhibitor (ThermoFisher Ribolock or NEB Murine RNase Inhibitor) to prevent RNA degradation.
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bioRxiv - Molecular Biology 2019Quote: ... The Cap 1 25mer RNA was purified using Monarch RNA Cleanup kit (50 μg capacity, NEB). Complete methylation was verified by digestion of RNA with the Nucleoside Digestion Mix (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1 μg of linearised DNA was then used in a HiScribe T7 synthesis kit (NEB), before DNase treatment and purification using a PureLink RNA mini kit (Thermo Fisher).
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bioRxiv - Bioengineering 2019Quote: ... The inserts were ligated to the phPB52 digested vector in 1× T4 DNA ligation buffer (NEB) with 30 ng of vector DNA with three molar excess of synthetic inserts ...
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bioRxiv - Molecular Biology 2019Quote: ... We used the NEBNext Multiplex Oligos for Illumina (Index Primers #1 - #24) (NEB, E7335S and E7500S) as the unique index primers ...
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bioRxiv - Microbiology 2019Quote: ... 1 μl Exonuclease I and 2.44 μl Antarctic Phosphatase buffer (New England BioLabs GmbH, Frankfurt, Germany) to 20 μl of the pooled PCR product ...
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bioRxiv - Biochemistry 2019Quote: Assays were conducted by incubating 0.1 and 1 μM SidJ in 1X Protein Kinase buffer (NEB), with 10 mM CaCl2 ...
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bioRxiv - Microbiology 2019Quote: ... 600 ng of total RNA were mixed with 0.83 μL 100 mM tris pH 7.5 and 0.17 μL 3 mg·mL−1 Random Primers (NEB) to a volume of 5.25 μL ...
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bioRxiv - Molecular Biology 2020Quote: ... After incubation for min at 55°C 1 μl T4 ligase (400,000 units/ml, NEB, M0202) was added ...
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bioRxiv - Genomics 2019Quote: ... Ligation was then performed in a ratio of 1:50 (vector:insert) with T4 DNA ligase (NEB) at room temperature for one hour and then transformed into NEB10 competent cells (NEB) ...
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bioRxiv - Genetics 2019Quote: ... 1 uL of cDNA or gDNA was amplified using Q5 High Fidelity 2X Master Mix (NEB). An indexing PCR was performed next using Nextera XT index kit primers (Illumina ...
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bioRxiv - Genomics 2021Quote: ... 500 ng of DNA was dissolved in 9 μL of 1 × CutSmart buffer (New England Biolabs) and sheared by pipetting 30 times with a pipette set at 8 μL with a P2/P10 tip ...
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bioRxiv - Microbiology 2021Quote: ... The pGHJ-TgApiAT6-1 and pGHJ-TgApiAT1 [27] plasmids were linearized by digestion with NotI (NEB) for 2 hr ...
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bioRxiv - Bioengineering 2020Quote: A 10x Cas12-gRNA complex mixture was prepared by pre-incubating 1 μM of LbCas12a (NEB) with 1.25 μM gRNA in 1x NEBuffer 2.1 at 37°C for 30 min ...
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bioRxiv - Biochemistry 2021Quote: ... coli cells (in 10.5 μl of nuclease-free water) were combined with 1 mM ATP (NEB), 40 U RNaseOUT ...
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bioRxiv - Biochemistry 2020Quote: ... the amplified DNA and the P4H-TM isoform 1 plasmid were both digested with BlpI (NEB) and XbaI (NEB ...
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bioRxiv - Neuroscience 2020Quote: ... and 7.5 μl of 1:1330 diluted micrococcal nuclease (11.3 gel units, New England Biolabs: NEB) for 5 min at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... and 7.5 μl of 1:1330 diluted micrococcal nuclease (11.3 gel units, New England Biolabs: NEB) for 5 min at 37 °C ...