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Citations for New England Biolabs :
3101 - 3150 of 6023 citations for 7 Oxa 1 2 diazaspiro 4.4 non 1 en 6 one 4 methyl cis 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... The gRNA binding regions were checked for presence of single nucleotide polymorphism (SNP) by amplifying the region using One Taq 2X master mix (New England Biolabs, Ipswich, Massachusetts, USA) and subsequent sanger sequencing of the PCR product (primers designed by primer-BLAST ...
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bioRxiv - Molecular Biology 2021Quote: ... One microgram of this PCR product and pKLV-U6gRNA(BbsI)-PGKpuro2ABFP was digested with MluI and BamHI (New England Biolabs, Ipswich, MA, USA). The digested products were purified ...
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bioRxiv - Genomics 2023Quote: ... The strain CBS 411.78-was prepared and sequenced with the ligation kit SQK108 in a one-pot reaction using 500 ng DNA for end prep and ligation (NEB Ultra-II ligase) and sequenced on an R9.4.1 flowcell ...
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bioRxiv - Genomics 2022Quote: ... and one short read library using the NEBNext Ultra II FS DNA Library Kit for Illumina (New England BioLabs Inc., Ipswich, MA, USA). The long-read library was then sequenced on the PacBio Sequel IIe system in CLR mode using the Sequel II Binding Kit 2.2 (PacBio) ...
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bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 viral RNA detection and quantification was performed using the Luna Universal Probe One-Step RT-qPCR kit (New England Biolabs, Whitby, ON, Canada) on the Rotor-gene Q platform (Qiagen) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Then total RNA (500 ng per sample) was purified by one round of polyA mRNA selection with oligo-dT magnetic beads (NEB, Cat. No. S1550S), converted to cDNA using KAPA mRNA HyperPrep kit (KAPA biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... falciparum in placenta samples was evaluated using One Taq® Quick-Load® 2X Master Mix with Standard Buffer (NEB, Cat No. M0486L) and the following thermocycler program ...
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bioRxiv - Biophysics 2021Quote: ... 25 μL of PCR reaction was mixed with 1 μL of DpnI (NEB, cat#R0176) at 37°C for 4 hours ...
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bioRxiv - Neuroscience 2021Quote: ... Chromatin was digested in situ using five units of restriction enzyme CviKI-1 (NEB, R0710S) every 100.000 nuclei and RELACS custom barcodes (4 bp UMI + 8 bp RELACS barcode ...
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bioRxiv - Microbiology 2019Quote: PCR mixtures were digested for 1 h at 37 °C with DpnI (New England Biolabs) to remove the template plasmids ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then stained with 1 µM of SNAP-Surface-AlexaFluor 647 (BG-A647, NEB) in 4% BSA + 0.1% Tween-20 for 1 hour at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... the dUTP-containing second strand was digested by adding 1 µL of USER enzyme (NEB) and incubating at 37 °C for 15 minutes ...
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bioRxiv - Genomics 2019Quote: ... bead-nuclei were resuspended in 190 µL RNA ligase solution (1× RNA ligase buffer (NEB), 4.5 µM bridge adapter ...
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bioRxiv - Genomics 2019Quote: ... supplemented with 0.1% Triton X-100 and with 200 µL 1× RNA ligase buffer (NEB) by repeating resuspending/pelleting.
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bioRxiv - Cell Biology 2019Quote: ... 1) Genomic DNA was digested with 200U HindIII-HF (New England BioLabs, prod. number R3104S) at 37°C overnight ...
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bioRxiv - Biophysics 2019Quote: ... 25 µL of PCR reaction was mixed with 1 µL of DpnI (NEB, cat# R0176), 1 µL of T4 PNK (NEB ...
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bioRxiv - Cell Biology 2019Quote: ... The supernatant was applied onto a column with 1 ml of equilibrated amylose resin (NEB). The column with the beads and the extract was incubated on a rotating wheel at 4°C for 1 h ...
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bioRxiv - Synthetic Biology 2019Quote: ... We added 1 µL of murine RNase inhibitor to all in vitro reaction (NEB# M0314S). Each reaction also received 60 pmol of either the RED20-encoded or the standard-encoded expression vector ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 1 μg of DNA was digested at 37C with the restriction enzyme EcoRI-HF (NEB) for two hours after which MspI (NEB ...
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bioRxiv - Cancer Biology 2019Quote: ... 1× protease inhibitor cocktail) and incubated with 12,000 gel units/ml micrococcal nuclease (NEB, M0247S) at 37 °C with frequently mixing to digest chromatin to lengths of approximately 100-to-1,000 bp ...
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bioRxiv - Biophysics 2019Quote: ... using a 5-fold excess of fluorophore to protein and ∼1 μM Sfp synthase (NEB) for 16 hours at 6 °C in 50 mM TRIS-HCl pH 7.5 ...
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bioRxiv - Genetics 2019Quote: ... Using the 100 bp and 1 kb DNA Quick-Load ladders (N0467 and N0468, NEB) as a reference ...
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bioRxiv - Genetics 2021Quote: ... 1 μl of 10 mM dNTPs and 0.5 μl of OneTaq Polymerase (New England BioLabs). To generate (partially ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 1 μL cDNA was used in a 30-cycle PCR using Q5 DNA Polymerase (NEB) with primer 8 & 9 or 10 & 11 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 1 μL cDNA was used in a 30-cycle PCR using Taq DNA Polymerase (NEB) with primer 4 & 6 or 5 & 6 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Purified products were ligated with 1 µL T4 DNA ligase (protocol as per NEB®) for 10 minutes at room temperature (benchtop ...
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bioRxiv - Bioengineering 2021Quote: ... for 16 cycles annealing at 67 °C was mixed with 1 μL of ExoI (NEB) digested mixture as the template ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μg standard glycoprotein was mixed in 1 μL of 10× glycoprotein denaturing buffer (NEB) (final conc ...
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bioRxiv - Biochemistry 2020Quote: ... 5:1 molar ratio of insert to backbone and 1X Gibson Assembly Master Mix (NEB) and incubated at 50°C for 1 hour before transformation into One Shot TOP10 Chemically Competent E ...
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bioRxiv - Biochemistry 2020Quote: ... 5:1 molar ratio of insert to backbone and 1X Gibson Assembly Master Mix (NEB) and incubated at 50°C for 1 hour before transformation into One Shot TOP10 Chemically Competent E ...
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bioRxiv - Plant Biology 2021Quote: ... total or virion DNA was treated with 1 unit of Mung Bean nuclease (NEB, Massachusetts). The digestion products were analyzed for SEGS-2 episomes as described above.
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bioRxiv - Microbiology 2020Quote: ... T5 exonuclease diluted 1:5 with 5X reaction buffer (New England BioLabs; 0.01 units/μL), Taq DNA ligase (New England BioLabs ...
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bioRxiv - Genetics 2021Quote: ... 250 ng – 1 μg of RNA samples were digested with Nucleoside Digestion mix (NEB, M069S) for 2 hr at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... PBT was removed and SNAP substrate diluted in PBT (SNAP-Surface649, NEB S9159S; 1:1000) added ...
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bioRxiv - Genomics 2021Quote: ... into 96-well plate containing 2.5μl of methylase reaction buffer (1 × M.CviPI Reaction buffer (NEB), 2 U M.CviPI (NEB) ...
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bioRxiv - Cell Biology 2021Quote: ... and 70 μl water and 1mM MnCl2 added together with 1 μl DNaseI (NEB #M0303S) for 10 minutes at room temperature in order to degrade any DNA that precipitated overnight ...
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bioRxiv - Developmental Biology 2022Quote: ... total RNA (1 µg) extracted from adult mouse testes was treated with alkaline phosphatase (NEB), de-phosphorylated ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were recovered in 1 ml NEB® 10-beta/Stable Outgrowth Medium (NEB, B9035) at 37 °C for 1 hour ...
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bioRxiv - Genetics 2022Quote: ... The supernatant (~12 ml) was added to 1 ml HEGX washed chitin magnetic resin (NEB) and left to bind for 1 h at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... the library was treated with a 1:20 dilution of USER enzyme (New England Biolabs) at 37 °C for 30 min ...
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bioRxiv - Developmental Biology 2021Quote: ... at least 1×106 fixed cells were digested using the DpnII restriction enzyme (NEB, R0543M). Chromatin was re-ligated with T4 ligase (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... with ISPCR (IDT; 0.1 μM) and Lambda Exo (New England BioLabs MO262S; 1 units/reaction) for 12 or 14 total cycles.
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bioRxiv - Microbiology 2021Quote: ... we treated the purified RT reaction with 1 μl of Exonuclease I (NEB, Catalog# M0293L) at 37°C for 30 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... (2019): 1) the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7760) was used for library preparation ...
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bioRxiv - Genomics 2022Quote: ... Each “PCR#1” reaction contained 25 µL of Q5 High-Fidelity 2X Master Mix (NEB), 2.5 µL of Nuc PCR#1 Fwd Primer (10 µM) ...
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bioRxiv - Biochemistry 2022Quote: ... 5 μl of 2x Quick Ligase buffer and 1 μl of Quick ligase (NEB, M2200) in a final volume of 10 μl was incubated for 10 min at RT and then used to transform DH5α competent cells ...
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bioRxiv - Biochemistry 2019Quote: ... per 1µg protein for 1 hour at 30°C in PMP buffer (New England Biolabs) plus 1mM MnCl2 ...
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bioRxiv - Microbiology 2019Quote: ... The plasmid was cut and de-phosphorylated simultaneously using 1 U Shrimp Alkaline Phosphatase (NEB) and 20 U EcoRV-HF (NEB ...
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bioRxiv - Genomics 2019Quote: ... mRNAs were isolated from 1 μg of total RNA using a PolyA selection kit (NEB) and sequencing libraries were prepared using the NEBnext Ultra RNA library prep kit for Illumina (NEB) ...
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bioRxiv - Genetics 2019Quote: ... mRNAs were isolated from 1 μg of total RNA using a PolyA selection kit (NEB) and sequencing libraries were prepared following instructions from NEBs Ultra Library Preparation Kit for Illumina ...