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Citations for New England Biolabs :
3301 - 3350 of 8151 citations for 6H Pyrazolo 3 4 b pyridin 6 one 3 cyclobutyl 1 2 4 5 tetrahydro 4 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 5 μg purified LbCas12a (New England BioLabs, catalog# M0653T) was incubated with equal molar purified gRNA (near 0.5 μg ...
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bioRxiv - Biochemistry 2021Quote: ... 5 uL of Taq Polymerase (5,000 U/mL) (NEB), and 100 – 300 ng of DNA template ...
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bioRxiv - Cancer Biology 2021Quote: ... before 5’ radiolabeled using T4 PNK (New England Biolabs). Labeling reactions were purified with acid-phenol chloroform and chloroform before precipitated overnight with ethanol.
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bioRxiv - Genomics 2022Quote: ... 5 µL of 10X VCE Buffer (New England Biolabs) and 0.5 units of yeast pyrophosphatase (New England Biolabs) ...
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bioRxiv - Molecular Biology 2022Quote: ... 5’ phosphorylated by T4 polynucleotide kinase (New England Biolabs), ligated to a blunt-end linear vector (typically also PCR products) ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5 U/μl T7 RNA polymerase (New England Biolabs), 1 U/μl SUPERase In (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2022Quote: ... and 5 U/µl T7 RNA polymerase (NEB M0251) in 1x RNAPol buffer (NEB) ...
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bioRxiv - Synthetic Biology 2024Quote: ... After 5 PCR cycles using Q5 polymerase (NEB #M0491) with 5X GC Buffer with a melting temperature of 72°C ...
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bioRxiv - Developmental Biology 2024Quote: ... 5 U BsmBI (New England Biolabs, Cat. No. R0580), 3 U BglII (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... and 5% v/v PEG 8000 (New England Biolabs) during ligation.
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bioRxiv - Molecular Biology 2024Quote: ... 8 μl 5 U/μL Klenow Fragment (NEB, M0210S) and 2 μl T4 PNK (NEB ...
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bioRxiv - Biophysics 2024Quote: ... and Monarch PCR&DNA Cleanup Kit (5 μg) (NEB). The purified dsDNA templates were transcribed using HiScribe T7 High Yield RNA Synthesis Kit (NEB ...
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bioRxiv - Microbiology 2024Quote: ... then incubated with 5 units of Antarctic phosphatase (NEB) at 37°C overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5 μM Mth RNA Ligase (New England Biolabs) was split into two 50 μl aliquots in thin-walled PCR tubes and incubated at 65°C in a thermal cycler with a heated lid set to 105°C for 1 hour ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5 U/μl Protoscript II reverse transcriptase (NEB, M0368X), 1x Protoscript II reverse transcriptase buffer ...
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bioRxiv - Plant Biology 2023Quote: ... 5 µl of 2X Luna qPCR Master Mix (NEB), 0.02 mM of forward and reverse primer ...
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bioRxiv - Biophysics 2023Quote: ... myosin-5 was incubated with apyrase (New England Biolabs) at 25 units / mL working concentration at room temperature for 10 min before being added to the well ...
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bioRxiv - Bioengineering 2023Quote: ... coli strains: NEB 5-alpha (NEB, C2987; not authenticated), BL21-AI (Thermo Fisher ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by 5’ phosphorylation with T4 Polynucleotide Kinase (NEB) and self-ligation with T4 DNA Ligase (NEB).
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bioRxiv - Genomics 2023Quote: ... +28 μl M.EcoGII at 5 U/μl (NEB M0603S). The DNA was purified using Qiagen PCR columns and quantified by measuring A260 ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were transformed into NEB 5-alpha F’Iq (NEB). Standard chemically competent Escherichia coli transformation protocols were used to construct plasmid host strains ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by addition of 5 units Antarctic Phosphatase (NEB) and 2.5 µL 10 × Antarctic Phosphatase buffer ...
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bioRxiv - Physiology 2024Quote: ... 5 μL of O-glycosidase (40,000 U/μL, NEB) with 1x GlycoBuffer 3 (NEB) ...
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bioRxiv - Cancer Biology 2024Quote: ... and transformation (chemically competent NEB 5-alpha E. coli) were performed according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... 5’ phosphorylated using T4 polynucleotide kinase (New England Biolabs), ligated by the addition of T4 DNA ligase and transformed in E ...
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bioRxiv - Biochemistry 2024Quote: ... Coli NEB® 5-alpha aliquot (NEB®, #C2987H). Plasmids were sanger sequenced by GeneWiz (Azenta Life Sciences ...
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bioRxiv - Biochemistry 2024Quote: ... Coli NEB® 5-alpha aliquot (NEB®, #C2987H). Plasmids were sanger sequenced by GeneWiz (Azenta Life Sciences ...
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bioRxiv - Synthetic Biology 2024Quote: ... 5 µl of 10x T4 ligase buffer (NEB B0202S), 1 µl of T4 PNK ...
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bioRxiv - Biochemistry 2021Quote: ... sDrl-2 for crystallization was also partially deglycosylated with PNGase F (New England BioLabs: 2,000 unit/mg sDrl-2) for 3 h at room temperature before sizing.
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bioRxiv - Systems Biology 2019Quote: ... the samples were added to 2 μL of second-strand synthesis mix (2.5× NEB buffer 2 [New England Biolabs] ...
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bioRxiv - Biochemistry 2021Quote: ... 2 μl of this lysate was directly used for PCR reactions with 2× Taq start master mix (M0496L, NEB), and the two NASP clone screening primers (see primer table) ...
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bioRxiv - Synthetic Biology 2022Quote: ... These were phosphorylated (2 μL 100 μM oligo stock, 2 μL 10X T4 DNA ligase buffer (New England Biolabs), 1 μL T4 PNK (New England Biolabs) ...
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bioRxiv - Molecular Biology 2023Quote: ... 200 nM dT adaptor primer and 200 nM Vλ1-GSP4-2-Hind III and 2 U Taq polymerase (NEB), in a final volume of 50 µl ...
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bioRxiv - Microbiology 2020Quote: ... bacilliformis total RNA (for each 25 µl reaction) collected from various conditions using the Luna Universal One-Step RT-qPCR kit (New England BioLabs; Ipswich, MA) according to the manufacturer ...
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bioRxiv - Biochemistry 2020Quote: ... and peptides in one aliquot were deglycosylated by further treatment with 125 U of Peptide-N-Glycosidase F (PNGase F) (New England BioLabs, MA, USA) for 16 h at 37 °C.
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bioRxiv - Microbiology 2019Quote: ... and also quantified using NEB Luna Universal qPCR Master Mix and Luna Universal One-Step RT-qPCR Kit (New England Biolabs, Ipswich, MA) for qPCR and RT-qPCR test ...
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bioRxiv - Plant Biology 2021Quote: ... One μg of total RNA was used in NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, Frankfurt, Germany), followed by sequencing to obtain 14 to 29 mio ...
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bioRxiv - Microbiology 2022Quote: ... Reverse-transcription polymerase chain reaction (RT-PCR) was performed using the Luna Universal Probe One-Step RT-qPCR kit (New England BioLabs; https://www.neb.ca). The 5 ‘ untranslated region (UTR ...
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bioRxiv - Neuroscience 2021Quote: ... RT-qPCR was performed on a CFX Connect thermocycler with the Luna Universal or Probe one-step qPCR Master Mixes (New England Biolabs, Ispwich, MA). RNA (1-4 ng ...
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bioRxiv - Microbiology 2020Quote: ... PCRs were performed in a final volume of 20 μl including NEB Luna Universal Probe One-Step Reaction Mix and Enzyme Mix (both New England Biolabs, Herts, UK) and 5 μl of RNA sample ...
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bioRxiv - Microbiology 2019Quote: ... Measurement of differential gene expression was conducted using a qTower 2.2 (Analytik Jena, Jena, Germany) with the Luna® Universal One-Step RT-qPCR Kit (New England Biolabs, Ipswitch, USA). Primer pairs used for the analysis are listed in Table S3 D ...
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bioRxiv - Microbiology 2021Quote: ... mixtures for the detection of viral RNA were made by a Luna® Universal Probe One-Step RT-qPCR Kit (New England Biolabs, USA) in accordance with the manufacturer’s instructions and then placed in a thermocycler (BIO-RAD CFX96™ Real-Time System ...
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bioRxiv - Immunology 2022Quote: ... as described by Corman et al84 were used alongside the Luna Universal One-Step RT-qPCR kit (New England Biolabs, Ipswitch, MA) and CFX384 Touch Real-Time System (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... We designed spCas9 guide RNAs to target codon 12 of the human KRAS gene and selected the one with highest targeting efficiency determined by T7E1 mismatch assay (EnGen Mutation Detection Kit, New England Biolabs, Ipswich, MA). Truncated guide RNA was produced by PCR assembly of a guide RNA template (56 ...
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bioRxiv - Biochemistry 2024Quote: ... 2 µg of genomic DNA was digested with one of few combinations of restriction enzymes (Table S5, all enzymes from NEB, Ipswich, MA). Digested genomic DNA was ligated using T4 ligase (NEB ...
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bioRxiv - Genomics 2023Quote: ... The Trial 2 pool was assembled by merging untagged extracted DNAs from each individual in equimolar concentration. One large insert (avg. 650 bp) NebNext Ultra II library (New England Biolabs, Ipswich, MA) was generated for each of the two Trial 1 parents (SS1773 and x SS3159 ...
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bioRxiv - Physiology 2023Quote: ... RT-qPCR (quantitative reverse transcriptase PCR) was then performed using a Luna® Universal One-Step RT-qPCR Kit (New England Biolabs; E3005) per manufacturer’s instructions and primers for the CaSeq1 gene of interest (IDT ...
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bioRxiv - Cancer Biology 2023Quote: sgRNA were directly amplified from genomic DNA in one step PCR using NEBNext® Ultra™ II Q5® Master Mix (NEB). Each PCR reaction was done with 10µg of genomic DNA in 100µL final volume ...
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bioRxiv - Neuroscience 2024Quote: ... and 50 ng of total RNA was used for RT-qPCR using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs, cat# E3005L) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 0.75 µl End-prepped DNA, 1 µl Native Barcode (Native Barcoding Expansion 96, EXP-NBD196) and 5 µl Blunt/TA Ligase Master Mix (NEB Blunt/TA Ligase Master Mix, M0367) were combined in a new reaction vessel and incubated for 20 minutes at room temperature ...