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Citations for New England Biolabs :
3151 - 3200 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... PCR reactions were performed using Phusion polymerase (New England Biolabs), and the PCR products were gel-purified from the product mixtures to eliminate non-specific PCR products ...
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bioRxiv - Genomics 2021Quote: ... After purification with Monarch PCR & DNA Cleanup kit (NEB, #T1030S), libraries were indexed using NEBNext Multiplex Oligos (NEB ...
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bioRxiv - Synthetic Biology 2021Quote: ... All enzymes for PCR and cloning were obtained from NEB. All plasmids were cloned via Gibson assembly (26) ...
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bioRxiv - Molecular Biology 2020Quote: Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB) was used to amplify the novel and annotated transcript using custom primers (IDT ...
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bioRxiv - Neuroscience 2020Quote: ... 12.5 μL NEBNext High-Fidelity 2× PCR Master Mix (NEB) were added and samples were PCR-amplified (72 °C 5 min ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were digested with MseI (NEB, Frankfurt/Main, Germany), and resolved on 15% PAGE ...
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bioRxiv - Microbiology 2020Quote: ... Purified PCR product was digested with restriction enzyme HinFI (NEB) for 1 hour and resolved on 1.2% agarose gel ...
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bioRxiv - Biophysics 2020Quote: All PCRs were made using either Phusion or Q5 (NEB). PCR reaction were in a volume of 50 μl and included water ...
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bioRxiv - Genomics 2020Quote: All PCR reactions were performed using Q5 polymerase (NEB M0491S) according to manufacturer protocols ...
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bioRxiv - Cell Biology 2022Quote: ... and 2x Q5 Hot Start PCR Master Mix (NEB M094). In total ...
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bioRxiv - Molecular Biology 2022Quote: ... plus 15µL NEBNext 2x HiFi PCR MasterMix (New England Biolabs) and thermocycling with the following conditions ...
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bioRxiv - Molecular Biology 2022Quote: ... HERVH1env ORF was PCR-amplified using Q5 polymerase (NEB, M0491L) from HeLa and 293T genomic DNA respectively and cloned into a TOPO vector (ThermoFisher ...
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bioRxiv - Synthetic Biology 2022Quote: ... Correct PCR products were digested with DpnI (New England Biolabs) to remove the template and subsequently cleaned up with a DNA cleanup kit (Zymo Research ...
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bioRxiv - Systems Biology 2022Quote: ... 25μL NEB Next HiFi PCR Mix (2x solution, NEB M0544), 10μl H2O) ...
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bioRxiv - Microbiology 2022Quote: ... by PCR with Q5 polymerase (New England Biolabs – MA, USA), and primers (Table S3 ...
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bioRxiv - Genetics 2022Quote: ... and then PCR-amplified using Taq polymerase (New England Biolabs) and primers listed in S1 Table ...
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bioRxiv - Plant Biology 2022Quote: ... PCR products were digested with BseYI enzyme (New England BioLabs) according to the manufacturer’s protocol and analyzed by gel electrophoresis to determine the genotype.
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bioRxiv - Microbiology 2022Quote: ... PCR amplification was performed using Phusion Polymerase (New England Biolabs). Template material was metagenomic DNA from feces ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR products were digested with ApoI-HF (New England Biolabs) and run on a 2% TAE agarose gel ...
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bioRxiv - Neuroscience 2020Quote: ... Targeting was verified by PCR using LongAmp HotStart polymerase (NEB) using primers listed in Table S1.
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Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imagingbioRxiv - Molecular Biology 2021Quote: ... PCR reactions were performed with Q5 polymerase (New England Biolabs) in the buffer provided ...
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bioRxiv - Cell Biology 2020Quote: ... PCR amplifications were performed using Phusion polymerase (New England Biolabs), products were analyzed by agarose gel electrophoresis and detected using RedSafe/UV light ...
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bioRxiv - Genomics 2022Quote: ... while four were amplified via Q5 PCR (New England Biolabs) with forward primer RA199 and oligo-specific primers to append the guide sequence (primers RA213-216 ...
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bioRxiv - Molecular Biology 2020Quote: ... and looking for PCR bands acquiring resistance to BbsI (NEB) cutting and or bigger insertion/deletions (indels) ...
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bioRxiv - Genetics 2022Quote: We used a multiplex colony PCR with Taq polymerase (NEB) and oligos oHJC120 ...
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bioRxiv - Genetics 2019Quote: The oligonucleotide pool was PCR-amplified using Phusion Polymerase (NEB) and primers described in Supplementary Table 4 ...
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bioRxiv - Genetics 2019Quote: ... These three fragments were PCR-amplified individually (New England Biolabs Q5 high-fidelity 2X master mix ...
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bioRxiv - Developmental Biology 2019Quote: ... Amplified PCR products underwent digestion with AvaI (New England Biolabs), which generated two bands ...
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bioRxiv - Pathology 2019Quote: ... The resulted cDNA was PCR enriched using Phusion polymerase (NEB) with cycling parameters ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were digested with restriction enzymes (New England Biolabs) and ligated using T4 DNA ligase (New England Biolabs) ...
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bioRxiv - Plant Biology 2020Quote: ... All PCRs were done with Q5 High-Fidelity Polymerase (NEB) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... using the NEBNext High-Fidelity 2X PCR Master Mix (NEB) and in-house single indexing primers (Lamble et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... and amplified via PCR with NEBNext Hi-fidelity enzyme (NEB). The resulting next-generation sequencing libraries were sequenced on a HiSeq2500 (Illumina).
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bioRxiv - Plant Biology 2021Quote: ... DNA was purified using a Monarch PCR purification kit (NEB). qPCR for three independent replicates was performed using iQ SYBR Green Supermix (Biorad ...
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bioRxiv - Bioengineering 2020Quote: ... PCRs were performed with Q5 Master Mix (New England Biolabs) and primers NGS_p1_F and NGS_p1_R ...
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bioRxiv - Systems Biology 2019Quote: ... The PCR products were treated with Exonuclease 1 (NEB, M0293L) for 1 h at 37°C and purified by ethanol precipitation ...
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bioRxiv - Systems Biology 2019Quote: ... PCR products were treated with 40U Exonuclease 1 (NEB, M0293L) for 1 h at 37°C to remove free oligonucleotide and purified by ethanol precipitation/ or the DNA Clean and Concentrator 5 kit (Zymo Research ...
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bioRxiv - Microbiology 2020Quote: PCR was performed using Phusion HF polymerase (New England Biolabs). All restriction enzymes and T4 polynucleotide kinase were purchased from New England Biolabs and used according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... and purified using the Monarch PCR & DNA Cleanup Kit (NEB). Collected DNA was quantified with the Qubit dsDNA High Sensitivity Assay kit (ThermoFisher) ...
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bioRxiv - Plant Biology 2020Quote: ... The PCR products were digested with DpnI (NEB; Ipswich, USA) overnight and subsequently transformed into Escherichia coli DH5α ...
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bioRxiv - Synthetic Biology 2019Quote: ... and purified using the Monarch PCR & DNA Cleanup Kit (NEB). Note that a complete digestion of receiver vectors in both step 1 and 2 is key to avoid the recovery of empty backbones ...
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bioRxiv - Genetics 2021Quote: ... A second PCR using again the Phusion HiFi Polymerase (NEB) with a total of 14 cycles and an annealing temperature of 65°C was performed in order to attach the illumina adaptors and barcodes (Supp ...
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bioRxiv - Neuroscience 2021Quote: ... Knock-in lines were confirmed by PCR genotyping (Phusion, NEB) and Sanger sequencing (Genewiz) ...
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bioRxiv - Biochemistry 2021Quote: ... Semi-quantitative PCR was performed using Taq DNA Polymerase (NEB) and 15 ng cDNA.
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bioRxiv - Molecular Biology 2021Quote: ... PCR amplification was done using LongAmp Taq DNA polymerase (NEB). Next ...
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bioRxiv - Synthetic Biology 2021Quote: ... DNA was purified with Monarch PCR & DNA Cleanup Kit (NEB) and eluted in 20 µL of nuclease-free water ...
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bioRxiv - Genetics 2021Quote: ... PCR reactions (50 µl) containing 1 × Q5 reaction buffer (NEB), 200 µM dNTPs ...
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bioRxiv - Neuroscience 2020Quote: ... PCRs were performed using the Q5 polymerase (New England Biolabs) and specific primer pairs (Integrated DNA Technologies ...
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bioRxiv - Molecular Biology 2020Quote: All PCRs were made using either Phusion or Q5 (NEB). PCR reactions were in a volume of 50 μl and included water ...
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bioRxiv - Synthetic Biology 2021Quote: ... All PCRs were conducted with Q5 High-Fidelity Polymerase (NEB) following the manufacturer’s instructions ...