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Citations for New England Biolabs :
3101 - 3150 of 9358 citations for Rat Leptin RIA kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA was extracted with Monarch® HMW DNA Extraction Kit for Cells & Blood (NEB), digested with DpnI overnight at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... and DNase was removed using the Monarch RNA Cleanup Kit (50 µg; New England Biolabs). LunaScript RT SuperMix Kit (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... the NEBuilder HiFi Master Mix was used according to kit instructions (E5520S, New England Biolabs). All single integration plasmids were digested with PmeI before transformations and integrations were verified by PCR ...
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bioRxiv - Molecular Biology 2023Quote: ... 2019) and were constructed using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and the NEBNext Ultra II Directional RNA library Prep Kit for Illumina (New England Biolabs). For library amplification 17 or 24 PCR cycles were used ...
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bioRxiv - Molecular Biology 2022Quote: ... Synthesis was performed with the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Ph.D.™-12 Phage Display Peptide Library Kit (New England BioLabs® Inc., MA, USA) was amplified as following the recommended protocols by the manufacturer ...
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bioRxiv - Genomics 2023Quote: ... Sequencing library was constructed using the NEB Ultra library preparation kit (New England Biolabs, Ipswitch).
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bioRxiv - Molecular Biology 2023Quote: ... The reverse-transcription (RT) reactions were performed with 2X LunaScript® RT SuperMix Kit (NEB) with 5 μL of RNA input in 10 μL total volume ...
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Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeatsbioRxiv - Molecular Biology 2023Quote: ... RNAs were in vitro transcribed with HiScribe T7 ARCA mRNA Kit with tailing (NEB, E2060S) following the manufacturer’s recommended protocol ...
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bioRxiv - Plant Biology 2023Quote: ... RT-qPCR was performed using a Luna ® Universal One-Step RT-qPCR kit (NEB) in a CFX96 qPCR thermocycler (BioRad) ...
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bioRxiv - Microbiology 2023Quote: ... hansenii NQ5 was isolated using the Monarch HMW DNA Extraction Kit for Tissue (NEB T3060S) per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... or HiScribe® T7 High Yield RNA Synthesis Kit (New England BioLabs, cat. no. E2040S) following the manufacturers’ protocols ...
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bioRxiv - Cell Biology 2023Quote: ... the MBNL1 binding site (TGCTGC) was deleted using Q5 Site Directed Mutagenesis kit (NEB E0554). 12.5 ng of pFlareA-Mapt Ex4 splicing reporter was used as template and mixed with 12.5 µl of Q5 Hot Start High-Fidelity 2X Master Mix ...
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bioRxiv - Biochemistry 2023Quote: ... For assessment of HAC1spliced mRNA the Luna Universal Probe One-Step RT-qPCR Kit (NEB) was used with 2 µl of a 50 ng/ µl RNA sample ...
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bioRxiv - Synthetic Biology 2023Quote: ... was created using the NEBNext Ultra II DNA library prep kit for Illumina (NEB #7103S) with index primer set 1 (NEB #7335S ...
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bioRxiv - Genomics 2023Quote: ... and libraries were prepared with a NEBNext Ultra II Library Prep Kit (New England Biolabs).
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bioRxiv - Genomics 2023Quote: ... (Sacramento, CA, USA) for library construction (NEBNext Ultra RNA Library Prep Kit, New England Biolabs) from 1 µg total RNA and Illumina sequencing ...
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bioRxiv - Evolutionary Biology 2023Quote: ... size selection and PCR enrichment using the NEBNext Ultra Directional RNA Library Prep Kit (NEB). PCR enrichment step was performed with NEBNext Multiplex Oligos for Illumina (Dual Index Primers ...
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA was extracted from subconfluent cells with the Monarch total RNA purification kit (NEB), and reverse transcribed with LunaScript RT SuperMix (NEB) ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were prepared with NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs). Regions to sequence were selected with the SeqCap EZ Human Exome Library v3.0 (Roche Applied Science ...
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bioRxiv - Molecular Biology 2023Quote: ... the amplified oligo pool was gel- purified using a Monarch DNA Gel Extraction Kit (NEB). To generate the linearized vector for the light chain variant library ...
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bioRxiv - Molecular Biology 2023Quote: ... plasmids were edited via site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, NEB, Cat #E0554).
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bioRxiv - Neuroscience 2023Quote: ... using the NEBNext Ultra II RNA Library Prep with Purification Beads Kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... followed directly by using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB #E7760S) and NEBNext Multiplex Oligos for Illumina (NEB #E7600S ...
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bioRxiv - Synthetic Biology 2023Quote: ... IVT mRNA was produced using the HiScribe T7 High Yield RNA Synthesis Kit (NEB # E2040) along with 500 ng of linear template and 4 mM CleanCap AG Reagent (Trilink ...
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bioRxiv - Cell Biology 2023Quote: The libraries were constructed using NEBNext Ultra RNA Library Prep Kit for Illumina (E7530L, NEB). Sequencing was performed on an Illumina NovaSeq 6000 platform with 2D×D150 bp pair-end reads at Novogene ...
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bioRxiv - Plant Biology 2023Quote: ... The point mutant K665A SPY was generated via Q5 Site-Directed Mutagenesis Kit (NEB, E0552S). All plasmids were sequenced to ensure accuracy.
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bioRxiv - Physiology 2023Quote: ... Mutagenesis was done using the Q5® Site-Directed Mutagenesis Kit from NEB (Cat # E0554S). The sequence of oligonucleotides used for this mutagenesis reaction is available on request ...
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bioRxiv - Plant Biology 2023Quote: Libraries were prepared using NEBNext® Ultra™ II DNA Library Prep Kit (NEB, E7645) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Library preparation was carried out using NEBNext Ultra II DNA Library Prep kit (NEB E7645). All ChIP-Seq libraries were sequenced on an Illumina NextSeq500 platform.
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bioRxiv - Biophysics 2023Quote: ... Derivatives of pSB963 were altered using mutagenic primers and Q5 site-directed mutagenesis kit (NEB) to generate vectors containing CEN9 ...
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bioRxiv - Neuroscience 2023Quote: ... the indexed cDNA libraries were prepared using the NEBNext rRNA Depletion Kit (New England BioLabs), followed by NEBNext Ultra II Directional RNA Library Prep ...
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bioRxiv - Evolutionary Biology 2023Quote: ... DNA was concentrated using the Monarch PCR and DNA Cleanup kit (New England Biolabs, MA) with warmed elution buffer ...
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bioRxiv - Systems Biology 2023Quote: ... The resulting amplicon was purified by the Monarch PCR & DNA Cleanup Kit (New England Biolabs) and cloned into the pRDA-550 vector by NEBridge® Golden Gate Assembly Kit (BsmBI-v2 ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA libraries were prepared using NEBNext Ultra II RNA Library Prep Kit (New England Biolabs) with polyA enrichment and sequenced on a Novoseq S4 sequencer (Illumina ...
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bioRxiv - Genomics 2023Quote: ... All libraries were generated with NEBNext Ultra II Directional RNA library prep kit (NEB #E7760).
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bioRxiv - Neuroscience 2023Quote: ... 5-HT2A receptor mutants were designed and performed using Q5 mutagenesis kit (New England Biolabs). All DNA was sequence verified using Sanger nucleotide sequencing (Retrogen ...
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bioRxiv - Genomics 2023Quote: ... The sequencing library was constructed using an NEBNext DNA library prep kit (New England Biolabs), and 2×150 bp paired-end reads were sequenced using an Illumina NovaSeq 6000 by Novogene Corporation Inc ...
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bioRxiv - Cell Biology 2023Quote: ... and NEBNext® Ultra™ II RNA Library Prep Kit for Illumina (New England Biolabs). cDNA was sequenced using paired-end 150 bp chemistry on a HiSeq 4000 System (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... The resultant insert (5′-flank_SpecPromoter_kanR_3′-flank) was gel extracted (Monarch DNA GEL Extraction Kit, NEB) and added to competence peptide stimulated S ...
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bioRxiv - Genomics 2023Quote: ... The NEBNext Single Cell/Low Input cDNA Synthesis and Amplification Module kit (New England Biolabs) was used to generate cDNA from the extraction ...
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bioRxiv - Cancer Biology 2023Quote: ... we performed site-directed mutagenesis using Q5 site-directed mutagenesis kit (New England Biolabs Inc.) in the pMSCV-Abi1 Isoform 2 DNA plasmid and point mutated tryptophan at amino acid 485 in the SH3 domain to glutamine ...
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bioRxiv - Neuroscience 2023Quote: ... followed by cDNA synthesis using ProtoScript® Taq RT-PCR Kit [New England Biolabs (NEB), Ipswich ...
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bioRxiv - Biochemistry 2023Quote: ... These fragments were assembled using the NEBuilder HiFi Assembly kit into pMal-c6T (NEB #N0378S). The resulting construct encodes a TEV-cleavable 6xHis-MBP tag fused to two copies of the VanSB periplasmic domain ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial rRNA depletion was proceeded using the rRNA Depletion Kit (Bacteria) (New England BioLabs, #E7850L), and the residual mRNA was quality controlled and converted to the libraries that were sequenced on a NovaSeq 6000 (Illumina ...
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bioRxiv - Cell Biology 2023Quote: ... and the transcribed RNA was purified using the Monarch® RNA Cleanup Kit (NEB, T2040L), RNA concentration was measured ...
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bioRxiv - Biochemistry 2022Quote: ... All hAtg3 mutants were generated using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) with corresponding primers listed in Extended Data Table 2 and verified by sequencing ...
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bioRxiv - Biochemistry 2023Quote: ... and E779W were generated with the Q5 Site-Directed Mutagenesis Kit (E0554, New England Biolabs). The primers were designed with the NEBaseChanger® tool (Table S8).
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bioRxiv - Neuroscience 2023Quote: ... using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NEB E7760), and following the standard protocol ...