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Citations for New England Biolabs :
2951 - 3000 of 9358 citations for Rat Leptin RIA kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... subtilis expression constructs was performed using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs) following the manufacturer’s procedures ...
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bioRxiv - Immunology 2020Quote: ... then transcribed in vitro using HiScribe™ T7 High Yield RNA Synthesis Kit (NEB, #E2040S). sgRNAs were purified by RNA Clean & Concentration™ (Zymo Research ...
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bioRxiv - Microbiology 2021Quote: DNA extraction from agarose gels was performed using Monarch DNA Gel Extraction Kit (NEB, USA). DNA isolation from reaction mixtures was performed using Monarch PCR & DNA Clean-up Kit (NEB ...
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bioRxiv - Genetics 2020Quote: ... the Trp 185 residue was changed to Arg using Q5 Quick Mutagenesis Kit (NEB #E0552) according to the manufacturer’s instructions
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bioRxiv - Immunology 2021Quote: ... Libraries were then constructed using the NEB Ultra II RNA custom kit (New England BioLabs) on an Agilent Bravo WS automation system followed by 14 cycles of PCR using KAPA HiFI Hot Start polymerase (KAPA Biosystems) ...
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bioRxiv - Immunology 2021Quote: ... Second strand synthesis was performed using the NEBNext Ultra II second strand synthesis kit (NEB). In vitro transcription was performed using the NEB HiScribe High Yield RNA synthesis kit ...
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bioRxiv - Developmental Biology 2021Quote: ... sgRNA was then transcribed from this template using HiScribe T7 in-vitro transcription kit (NEB) and ...
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bioRxiv - Developmental Biology 2020Quote: ... Sequencing libraries were generated using NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Neuroscience 2020Quote: ... The cDNA libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB) and RNA was sequenced using the Illumina Novaseq PE150 platform.
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bioRxiv - Cell Biology 2021Quote: ... Synthesis was performed according to manufacturer’s instructions using the ProtoScript II Reverse Transcriptase Kit (NEB).
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bioRxiv - Cell Biology 2021Quote: ... PCR products were used directly as templates for IVT using HiScribe T7 transcription kit (NEB). Resulting RNA was purified using RNAClean XP beads (Beckman Coulter ...
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bioRxiv - Genetics 2020Quote: ... Total RNA extraction was carried out using the Monarch RNA Cleanup Kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... For the majority of isolates DNA was extracted using Monarch Genomic DNA Extraction kit (NEB), but in some instances a lower quantity and quality yield was obtained ...
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bioRxiv - Developmental Biology 2022Quote: ... Libraries were prepared using the FS DNA Library Prep Kit for Illumina sequencing (NEB, E7805). DNA was digested into 150 bp fragments and paired-end sequencing was performed using a NovaSeq 6000 Sequencing System ...
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bioRxiv - Neuroscience 2022Quote: ... synthesized into a large primer and inserted immediately downstream of the U6 promoter to generate pAAV.U6.shRNA.CMV.GFP.WPRE.hGH using the NEBuilder hifi DNA assembly cloning kit (New England Biolabs). Corresponding constructs with tdTomato as the reporter were generated by swapping in the 5’-MluI and 3’ AgeI U6-shRNA-CMV fragment from each pAAV.U6.shRNA.CMV.GFP.WPRE.hGH construct in place of the corresponding sequence in pAAV-U6-shRNA-CAG-tdTomato (Batool et al. ...
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bioRxiv - Genomics 2022Quote: ... The obtained fragments were library prepped using NEBNext Ultra II DNA Library Prep Kit (NEB) for Illumina ...
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bioRxiv - Cancer Biology 2022Quote: ... Libraries were prepared using the NEBNext Low Input RNA Library Prep Kit for Illumina (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... Total RNA was isolated with the Monarch Total RNA Miniprep Kit (New England Biolabs, NEB) following supplier’s recommendations ...
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bioRxiv - Genomics 2022Quote: ... Total RNA was isolated with the Monarch Total RNA Miniprep Kit (New England Biolabs, NEB) following supplier’s recommendations ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA concentrations were quantified by qPCR with the NEBNext Library Quant Kit for Illumina (NEB). Libraries were pooled and sequenced for 28-90 paired-end nucleotide reads to an average depth of ∼70,000 mapped fragments per cell.
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bioRxiv - Molecular Biology 2022Quote: ... in combination with the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770S). The NEBNext Adaptor for Illumina was diluted 1:5 in Tris/NaCl ...
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bioRxiv - Molecular Biology 2022Quote: ... using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB E7645) according to manufacturer instructions ...
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bioRxiv - Genomics 2022Quote: ... The NEBNext Ultra Directional RNA Library Prep Kit for Illumina (cat. # E7420L New England BioLabs) was used to prepare all RNA-seq libraries ...
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bioRxiv - Molecular Biology 2022Quote: ... ChIP libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Depletion of methylated host DNA was performed using NEBNext® Microbiome DNA Enrichment Kit (NEB) according to the manufacturer instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB #E7765) and NEBNext Multiplex Oligos for Illumina (NEB #E6440S and NEB #E6442S ...
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bioRxiv - Molecular Biology 2022Quote: ... reverse transcription was performed using ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs) using an anchored d(T ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR products were purified using a Monarch PCR & DNA Cleanup Kit (5 μg) from NEB and then visualized on an 0.8 to 1.2 % percent agarose gel resolved in Tris-acetate-EDTA electrophoresis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... the start codon was mutated to alanine using the Q5 Site-Directed Mutagenesis Kit (NEB). For C-terminal tagging ...
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bioRxiv - Bioengineering 2022Quote: ... mRNA was generated by in vitro T7 transcription (HiScribe™ T7 ARCA mRNA Kit, NEB). DNA and mRNA at the indicated concentrations were added to the mixture of cells and RT-PCR reagents immediately before proceeding to heat lysis.
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bioRxiv - Cell Biology 2022Quote: ... and the amplicons were extracted from a 1% agarose gel (Monarch Gel Extraction Kit, NEB). Inserts were then cloned into a pCDH1 lentiviral vector ...
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bioRxiv - Cell Biology 2022Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra RNA library kit for Illumina (NEB) following manufacturer’s specifications ...
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bioRxiv - Bioengineering 2022Quote: ... PCR products were purified using the Monarch® PCR & DNA Cleanup Kit (New England Biolabs) and submitted to the Massachusetts General Hospital DNA Core ...
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bioRxiv - Cancer Biology 2022Quote: ... DoxON-ETV6(R399C)-V5-GFP was generated with the Q5 site-directed mutagenesis kit (NEB) per manufacturer’s protocol.
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bioRxiv - Biochemistry 2022Quote: ... To complete the recombinant assembly reaction NEBuilder® HiFi DNA Assembly kit (New England Biolabs) was used with the combination of three different inserts produced by PCR ...
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bioRxiv - Biophysics 2022Quote: ... The protein was labeled with SNAP-Surface Alexa Fluor 647 labeling kit (New England Biolabs) according to the protocol provided by the manufacturer ...
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bioRxiv - Cell Biology 2022Quote: ... Sequencing libraries were prepared using the NebNext Ultra II DNA library kit (New England Biolabs), and sequenced using a paired-end strategy on a NextSeq500 instrument (Illumina).
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bioRxiv - Cell Biology 2022Quote: Total RNA of cell samples was extracted using the Monarch Total RNA kit (NEB, T2010S) and then reverse transcribed with iScript cDNA Synthesis kit (Biorad ...
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bioRxiv - Developmental Biology 2022Quote: ... GFP and Lphn2 were inserted into LV-UbC with Gibson assembly cloning kit (NEB E5510S). The Lphn2-F831A/M835A and Lphn2-T829G mutations were made using Q5 mutagenesis (NEB ...
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bioRxiv - Cell Biology 2022Quote: ... Site-directed mutagenesis was performed with the Q5 site-directed mutagenesis kit (New England Biolabs) or NEBuilder HiFi DNA assembly kit (New England Biolabs ...
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bioRxiv - Cell Biology 2022Quote: ... a NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB, E7645S) was used according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... a NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB, E7645S) was used according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... The PCRs were then purified with Monarch PCR & DNA Cleanup Kit (New England Biolabs, T1030) and Illumina adapters were added in another 2 rounds of PCR ...
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bioRxiv - Immunology 2022Quote: ... Libraries were quantified by qPCR using the NEBNext Library Quant kit for Illumina (NewEngland Biolabs) according to manufacturer’s protocol and pooled at equal molarity (1nM) ...
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Optimization and deoptimization of codons in SARS-CoV-2 and the implications for vaccine developmentbioRxiv - Evolutionary Biology 2022Quote: ... and assembled with the backbone using the NEBuilder HiFi DNA Assembly Cloning Kit (E5520S, NEB). All primers used in this study were listed in Supplementary Table S3.
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bioRxiv - Genomics 2022Quote: ... The library was prepared using NEBNext Ultra II FS DNA library prep kit (NEB E7805L) with 500 ng genomic DNA as input ...
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bioRxiv - Genomics 2022Quote: ... ChIP-seq libraries were prepared using NEBNext Ultra DNA Library Prep Kit for Illumina (NEB) following manufacture’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... the PCR product was transcribed using HiScribe T7 High Yield RNA Synthesis Kit (E2040S; NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Library preparation was performed according to NEBNext Ultra DNA Library prep kit instruction (NEB, E7370L) and samples were purified with magnetic AMPure bead (Beckman Coulter ...
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bioRxiv - Microbiology 2022Quote: ... using the NEBNext Ultra II FS DNA library prep kit (New England Biolabs, Frankfurt, Germany) to prepare a 2×300 bp library ...