Labshake search
Citations for New England Biolabs :
3101 - 3150 of 10000+ citations for PCR Genotyping kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... PCR was carried out with the Q5 DNA polymerase (New England Biolabs, USA, NEB #M0491). Primers were obtained from Eurofins Genomics (Germany ...
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bioRxiv - Plant Biology 2023Quote: ... Site-directed mutagenesis was performed using Phusion DNA polymerase PCR mix (New England Biolabs, UK). Primers were designed specifically to replace the nucleotides coding for serine at the 44th position in the CDS of OsbZIP1.1 with those for alanine as given in table S1.
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bioRxiv - Synthetic Biology 2023Quote: ... The PCRs were performed with Q5® High-Fidelity 2X Master Mix (New England Biolabs) with the designed synthetic primers (SigmaAldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... Each pooled library was PCR amplified using Phusion polymerase (F-530) (New England Biolabs, M0530S). Quality of the libraries was assessed with a Bioanalyzer using the High Sensitivity DNA Kit (Agilent ...
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bioRxiv - Microbiology 2023Quote: ... The PCR reaction mixture contained: 12.5 µL LUNA LongAmp Taq 2x Master Mix (NEB M0287S), 5.5 µL Nuclease-Free Water (ThermoFisher Scientific #AM9937) ...
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bioRxiv - Microbiology 2022Quote: ... cDNA libraries were obtained by PCR amplification for 15 cycles using Phusion DNA polymerase (NEB). After quantification with a micro fluorometer (TBS-380 ...
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bioRxiv - Microbiology 2022Quote: ... PCR fragments and linearized pDIA6754 (25) were mixed and assembled using Gibson Assembly (NEB, France) and transformed by heat shock in E ...
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bioRxiv - Molecular Biology 2023Quote: ... Diluted cDNA (100-fold) was used for two multiplex PCR reactions with Q5 polymerase (NEB) with two primer pools (Table S5) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Colony PCR was performed using Quick-Load Taq 2X master mix (New England Biolabs, M0271L). Successful colonies were grown in 100 μg/mL ampicillin-rich lysogeny broth (LB ...
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bioRxiv - Cell Biology 2023Quote: ... and mutant Pacer RH domain PCR product were mixed with Gibson Assembly master mix (NEB) in a 1:3:3 molar ratio prior to incubation at 50 °C for 15 minutes and transformation into XL10 E ...
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bioRxiv - Biophysics 2023Quote: ... the insert PCR fragment was phosphorylated with P4 polynucleotide Kinase (M0201, New England Biolabs, UK) and the destination PCR-fragment was DPN1 treated (R0176 ...
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bioRxiv - Genomics 2023Quote: ... PCR reactions were conducted with Phusion High-Fidelity DNA polymerase (New England Biolabs, no. M0530L) using the following cycling program ...
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bioRxiv - Molecular Biology 2023Quote: ... and (v) the four PCR fragments listed above were digested with DpnI (New England Biolabs), gel-purified ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmids encoding for GAF2 mutants were prepared by Q5 site-directed PCR mutagenesis (NEB) of pET28a-GAF2 using the primers listed in Table S1.
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bioRxiv - Bioengineering 2023Quote: ... The PCR-linearized plasmid was then circularized by the T4 ligase (NEB, Ipswich, MA, USA) to create pTargetF_araA ...
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bioRxiv - Genetics 2023Quote: ... two consecutive PCRs were performed by using the Q5 High-Fidelity DNA Polymerase (NEB, M0491). For the PCR 1 ...
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bioRxiv - Bioengineering 2023Quote: ... All PCR reactions were performed using Q5 HotStart DNA polymerase (NEB, Cat. No./ID: M0493L). Digestions were performed at 37C for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... PCR amplification of the libraries was performed using NEBnext Q5 Ultra II master mix (NEB) and the last sample cleanup was performed by double-sided PEG-magnetic bead purification to eliminate PCR primers and fragments above 1 kb in size ...
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bioRxiv - Cell Biology 2023Quote: ... Truncated ric-8 constructs were generated by PCR using high-fidelity Phusion DNA polymerase (NEB). Phosphorylation mutants were created using the QuikChange Lightning site-directed mutagenesis kit (Agilent Technologies) ...
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bioRxiv - Molecular Biology 2023Quote: ... Integrated sgRNA sequences were PCR amplified with Q5 high-fidelity DNA polymerase (New England Biolabs). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... PCR amplification was performed with Q5 polymerase according to the manufacturer’s instructions (New England BioLabs) and the following thermal cycling conditions ...
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bioRxiv - Microbiology 2023Quote: ... PCR reactions were performed with 2x OneTaq HotStart DNA Polymerase master mix (New England Biolabs), using 6 μl of forward and reverse primer (1μM each) ...
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bioRxiv - Synthetic Biology 2023Quote: ... The Phyper-spank device and the pTRKH2 were PCR-amplified using the Q5 polymerase (NEB) and cloned by Gibson assembly25 in E ...
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bioRxiv - Molecular Biology 2023Quote: ... ATAC-seq libraries were prepared using NEBNext High-Fidelity 2X PCR Master Mix (NEB, #M0541), a uniquely barcoded primer per sample ...
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bioRxiv - Cell Biology 2023Quote: ... PCR reactions were driven by the Phusion High Fidelity DNA polymerase (New England Biolabs (NEB), M0530) ...
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bioRxiv - Cell Biology 2023Quote: ... PCR reactions were driven by the Phusion High Fidelity DNA polymerase (New England Biolabs (NEB), M0530) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... g165 and g166 without the predicted signal peptides were PCR-amplified with Phusion polymerase (NEB) from genomic DNA of P ...
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bioRxiv - Genomics 2023Quote: ... All PCRs were run using Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs) according to the manufacturer’s protocol.
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bioRxiv - Plant Biology 2023Quote: ... Four fragments were amplified by PCR using the Q5 High-Fidelity DNA Polymerase (NEB #M0492) and purified using Monarch PCR & DNA Cleanup kit (NEB #T1030) ...
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bioRxiv - Bioengineering 2023Quote: ... PCR was performed using synthesized primers (Integrated DNA Technologies) and Q5 DNA polymerase (NEB, M0491). The sequences of the constructed plasmids were verified by Sanger sequencing (Azenta) ...
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bioRxiv - Plant Biology 2023Quote: Each PCR consisted of 2.5 µL 10X Standard Taq Reaction Buffer (NEB, Ipswich, MA, USA), 0.5 µL 10 mM dNTPs (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: Human ARHGAP18 constructs were created using Polymerase Chain Reaction (PCR) using New England Biolabs (NEB) Phusion High-Fidelity PCR Kit (catalog no ...
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: ... PCR amplification was performed with the specific primers and OneTaq master mix (New England Biolabs) under the following condition ...
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bioRxiv - Cell Biology 2023Quote: ... PCR reactions were performed by combining 12.5 µL Q5 high-fidelity 2× master mix (NEB), 2.5 µL each of 10 µM forward and reverse primers ...
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bioRxiv - Microbiology 2023Quote: ... and PCR carried out using Q5® High-Fidelity 2X Master Mix (New England Biolabs) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: All cloning PCR were done using Q5 High-Fidelity DNA Polymerase (New England Biolabs, #M0491S) and InFusion HD cloning according to manufacturer’s protocol (Takara Bio ...
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bioRxiv - Cell Biology 2023Quote: ... Full length or truncated DAAM1 constructs were PCR-amplified using Phusion High Fidelity Polymerase (NEB) and cloned into pCDNA4/TO or pCS2 expression vectors ...
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bioRxiv - Biophysics 2022Quote: ... ∼210 bp regions nearby the target protospacers were amplified via PCR with Q5 polymerase (NEB) and primers containing Illumina adaptor sequences ...
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bioRxiv - Cell Biology 2023Quote: ... Purified PCR products and pAcGFP1-N1 were incubated with XhoI and BamHI (New England Biolabs) under 37 °C for 4 hrs in 1X buffer 3 supplemented with 1% BSA ...
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bioRxiv - Genetics 2023Quote: ... PCR products were visualized on 1% agarose gels with a 1 kb plus ladder (NEB).
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bioRxiv - Developmental Biology 2023Quote: ... The PCR was done by using Q5 High-fidelity DNA Polymerase (New England Biolabs, M0491) in a mix carrying 10 μl Q5 PCR buffer (5x) ...
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bioRxiv - Developmental Biology 2023Quote: ... Homology arms (PCR products from genomic DNA) were inserted with Gibson assembly (New England Biolabs) into pDD287 (Addgene #70685) ...
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bioRxiv - Genomics 2023Quote: ... with different NEB-Next index primers and the NEB-Next Universal PCR Primer (NEB #E6861A) with three PCR cycles for MNAse samples or eight PCR cycles for ChIp samples ...
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bioRxiv - Microbiology 2023Quote: ... the immunity protein genes were amplified by PCR using Phusion polymerase (New England Biolabs, UK) with the following primers ...
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bioRxiv - Cell Biology 2023Quote: ... Library amplification PCRs were performed using NEBNext Ultra II Q5 Master Mix (New England Biolabs) on all the extracted genomic DNA to attach Illumina sequencing adaptors and barcodes ...
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bioRxiv - Genetics 2023Quote: DNA amplification was performed by PCR using Q5 Hot Start High-Fidelity DNA Polymerase (NEB) following the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: ... Subsequently the PCR samples (10µl) were digested with DPNI (0.2µl New England BioLabs GmbH, R0176S) in CutSmart Buffer to remove the wild-type background ...
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bioRxiv - Cell Biology 2023Quote: PCR reactions for cloning were performed using Q5 High-Fidelity DNA polymerase (New England Biolabs), according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Libraries were generated from two PCR reactions both using Q5 High-Fidelity DNA Polymerase (NEB). Primers flanking mutated regions amplified ...
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bioRxiv - Synthetic Biology 2023Quote: ... All in vitro PCR reactions were performed using Q5 High-Fidelity 2X Master Mix (NEB). Yeast colony PCR reactions were done as previously reported20 using DreamTaq DNA polymerase (ThermoFisher) ...