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Citations for New England Biolabs :
3101 - 3130 of 3130 citations for Locostatin CAS 90719 30 5 100% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... followed by with a buffer adapted from (5-9) consisting of 0.1% (1mg/mL) BSA (New England Biolabs, Ipswich, MA, Item No. B9000S) / 0.3% Igepal CA 630 (Sigma-Aldrich ...
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bioRxiv - Physiology 2022Quote: ... The single stranded cDNA was ligated with a partial Illumina 5’ adaptor (HZG885:/5phos/AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTddC) using T4 RNA ligase 1 (New England Biolabs, Ipswich, MA, US) and incubated overnight at 22 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... The fragment release step was performed in 5 μl 1% SDS supplemented with 1:10 Thermolabile Proteinase K (New England Biolabs cat. no. P8111S) at 37°C 1 hr followed by 58°C 1 hr ...
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bioRxiv - Cancer Biology 2023Quote: ... The fragment release step was performed in 5 μl 1% SDS supplemented with 1:10 Thermolabile Proteinase K (New England Biolabs cat. no. P8111S) at 37°C 1 hr followed by 58°C 1 hr ...
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bioRxiv - Microbiology 2024Quote: ... The initial dephosphorylation reaction was in a mixture (50 μL) containing 5 μL of terminal transferase buffer (NEB Tdt Reaction Buffer, Catalog # M0315S), 1 μL of shrimp alkaline phosphatase (rSAP ...
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bioRxiv - Microbiology 2024Quote: ... The initial dephosphorylation reaction was in a mixture (50 μL) containing 5 μl of terminal transferase buffer (NEB Tdt Reaction Buffer, Catalog # M0315S), 1 μL of shrimp alkaline phosphatase (rSAP ...
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bioRxiv - Evolutionary Biology 2024Quote: ... ssDNA standards were generated from PCR products amplified with a forward primer that was 5’ phosphorylated to promote Lambda exonuclease digestion of that strand (NEB, Cat. No. M0262S) and a reverse primer with phosphorothioate bonds between the first four 5’ nucleotides to block digestion ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Sequencing libraries were made with 5 ng DNA using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB #E7103S/L) following the manufacturer’s instructions ...
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Cis-regulatory divergence underpins the evolution of C3-C4 intermediate photosynthesis in MoricandiabioRxiv - Plant Biology 2021Quote: ... 17 μl total RNA (100 ng/μl) was added with 2 μl buffer and 0.5 μl RNase-free DNaseI enzyme (New England Biolabs GmbH, Frankfurt am Main, Germany) incubating on ice for 30 s ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 µL of linker ligation mixture was added (38% PEG-8000, 1x NEB T4 ligase buffer, 2 µM oBZ407_preA preadenylated linker, 100 units NEB T4 RNA ligase 2, truncated) and they were further incubated at 37 °C for 3 hrs ...
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bioRxiv - Microbiology 2021Quote: ... 500 μl of lysed cells were transferred to a tube containing 4.5 ml of digestion mix (1X NEB 3 buffer, 1% triton X-100) and 100 μl of the lysed cells were transferred to a tube containing 0.9 ml of digestion mix ...
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bioRxiv - Genomics 2020Quote: PCR products were loaded into a single lane on a 1% agarose gel with 100 bp DNA ladder (New England Biolabs, Frankfurt am Main, Germany). Fragments between 200 and 500 bp were cut from the gel with a scalpel and purified using the QIAquick gel extraction kit (QIAgen ...
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bioRxiv - Genomics 2020Quote: ... Between 100 and 200 ng of genomic DNA was digested using 2 units of the MslI restriction enzyme (New England Biolabs ®, Ipswich, Massachusetts, USA.) and using 1 unit of NEB4 buffer in 20 μl of volume and for 2 hours at 37 °C ...
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bioRxiv - Developmental Biology 2022Quote: The RNA-seq libraries were prepared from 100 μg of total RNA per genotype with NEBNext Ultra II RNA Library Prep Kit mRNA Isolation Module (NEB, Cat# E7775S, E7490S, E7500S). Quality control and sequencing of the RNA-seq libraries were performed by GENEWIZ ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 100 ng of DNA was cleaved with PstI HF and MseI restrictases in CutSmart® buffer (New England Biolabs; 3 hours at 37°C). P1 and P2 barcoded adapters corresponding to the restriction site of the enzymes were ligated immediately afterwards with T4 ligase (NEB ...
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bioRxiv - Genomics 2023Quote: ... Lysed cells were then diluted 10 time in several tubes containing 450 μl of digestion mix (1X NEB 1 buffer, 1% Triton X-100). 100 units of HpaII were added and the tubes were incubated for 2 hours at 37°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 5 µl of the dissolved cells were added to a 50 µl PCR reaction (Q5 DNA polymerase, NEB; see Table S2 for primer sequences) and amplified with 36 rounds of thermal cycling ...
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bioRxiv - Genetics 2021Quote: ... Genomic DNA (400 ng) was either mock digested (i.e. without enzyme addition) or digested with BglII (New England Biolabs, 5 units/rxn, NEBuffer 3.1) for 1 hour at 37°C ...
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bioRxiv - Plant Biology 2020Quote: ... A total of 12 sRNA libraries were constructed from 5 μg of size-selected RNA using the NEBNext® Small RNA Library Prep kit (New England Biolabs, Ipswich, MA, USA) as per the manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... After addition of 200 µl nuclease elution mix (NEB nuclease P1 buffer 1 x, MgCl2 5 mM, 0.5 µl NEB nuclease P1, 0.5 µg benzonase) samples were incubated over night at 37 °C ...
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bioRxiv - Genomics 2022Quote: ... and for the synthesis of the second strand of cDNA was used the Klenow fragment 3’-5’ exo (New England Biolabs Inc., Ipswich, MA, USA), following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... 100 ng purified linear vector fragments were assembled with 5 µl of annealed oligonucleotide pool using NEBuilder HiFi DNA Assembly (New England Biolabs, Ipswich, MA, USA, #E2621L) according to the manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: ... The reaction was performed in temperature cycles of 37°C for 2 min and 16°C for 5 min using the restriction enzyme Bsa I-HF® V2 and the Hi-T4™ DNA ligase (from New England Biolabs; Bioconcept, Allschwil, Switzerland), followed by a final 10 min ligation at 16°C ...
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bioRxiv - Biochemistry 2024Quote: ... A His-Tag encoding sequence was added to the C-terminus of RnfG by using oligonucleotides 5 and 6 via site-directed mutagenesis (Q5® Site-Directed Mutagenesis Kit, NEB, Frankfurt am Main, Germany). The resulting plasmid was checked by sequencing analysis (Microsynth Seqlab ...
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bioRxiv - Evolutionary Biology 2023Quote: ... in 20 µl reactions and products were run on a 1% agarose gel alongside an appropriately sized ladder (either New England Biolabs 100 bp or 1kb DNA ladder) and stained post-electrophoresis with GelRed® (Biotium) ...
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bioRxiv - Microbiology 2022Quote: ... 0.75 µl End-prepped DNA, 1 µl Native Barcode (Native Barcoding Expansion 96, EXP-NBD196) and 5 µl Blunt/TA Ligase Master Mix (NEB Blunt/TA Ligase Master Mix, M0367) were combined in a new reaction vessel and incubated for 20 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... 250 μL of 2 mg mL-1 Y289L GalT,46 50 μL of 500 kU mL-1 PNGase F (New England Biolabs, 5 U μg-1 of protein), and 62.5 μL of Lambda Protein Phosphatase (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... from 4 to 15 hours at 37 °C. For Circle-Seq of the CRISPR treatments and controls (Fig. 5) exonuclease T (New England Biolabs; 20 units per μg of DNA) was then added ...
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bioRxiv - Molecular Biology 2023Quote: ... using the manufacturer’s protocol or with radioactive labeling mix (1 µL 10x PNK buffer, NEB, 1 µL of 100 µM gapmer, 1 µL T4 PNK, NEB, 1 µL γ-32P-ATP, Hartmann, 6 µL ddH2O) for 40 min at 37°C ...
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bioRxiv - Physiology 2020Quote: ... Membranes were washed with TBS-T and then incubated with an anti-rabbit horseradish peroxidase conjugated secondary antibody (New England Biolabs, 1:10,000 in 5% skim milk in TBS-T) for 2h ...