Labshake search
Citations for New England Biolabs :
3101 - 3150 of 3625 citations for 5 Fluoro 2 piperidin 2 yl 1H benzimidazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... A circular form of the 3I_RAN FLEXI synthetic oligonucleotide control was generated by incubating the 5’ phosphorylated-3I_RAN FLEXI oligonucleotide (500 ng) with T4 RNA Ligase I (10 U; New England Biolabs) for 2 h at 25°C and 2 min at 95°C ...
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Recruitment of the m6A/Am demethylase FTO to target RNAs by the telomeric zinc finger protein ZBTB48bioRxiv - Molecular Biology 2024Quote: ... The immunoprecipitated RNA was 5’-end-labeled with 32P using T4 polynucleotide kinase (New England Biolabs catalogue number M0201L). Protein-RNA complexes were separated using 4–12% BisTris–PAGE and transferred to a nitrocellulose membrane (Protran) ...
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bioRxiv - Biochemistry 2024Quote: ... unlabeled RNAs were dephosphorylated at the 5’-end by treating with calf intestinal alkaline phosphatase (CIP, New England Biolabs) for one hour at 37 °C ...
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Recruitment of the m6A/Am demethylase FTO to target RNAs by the telomeric zinc finger protein ZBTB48bioRxiv - Molecular Biology 2024Quote: ... The immunoprecipitated RNA was 5’-end-labeled with 32P using T4 polynucleotide kinase (New England Biolabs catalogue number M0201L), separated using 4–12% BisTris–PAGE and transferred to a nitrocellulose membrane (Protran) ...
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bioRxiv - Cancer Biology 2024Quote: ... was used for tagmentation and amplified with Nextera Ad1_noMX and Ad2.X primers (Supplementary Table 5) using 2x Phusion high-fidelity PCR mix (NEB). AMPure beads (Beckman coulter ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 rounds of PCR were run using Q5® Hot Start High-Fidelity 2X Master Mix (NEB cat #M0494) following the manufacturer’s instructions with a 63°C annealing temperature and 30s extension ...
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bioRxiv - Biochemistry 2024Quote: RP library construction in brief: Footprint RNA was dephosphorylated using 5 U of T4 PNK (New England Biolabs, M0201S). Subsequently ...
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bioRxiv - Synthetic Biology 2024Quote: ... compatible overhangs for ligation were generated by digestion of PCR products using 5 units each of BsaI (NEB, R3733S) and DpnI (NEB ...
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bioRxiv - Plant Biology 2020Quote: ... APE1 gene with an additional 1500 bp on the 5’ side and 500 bp on the 3’ side was amplified by PCR using Q5 High-Fidelity DNA Polymerase (NEB) using primers 5’-TTATAATACCCACCCGTCAAAGCTGTG-3’ and 5’-TTATAACAGACTCATCCGGACCCCAA-3’ containing an additional PsiI restriction site (70°C ...
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bioRxiv - Biochemistry 2021Quote: ... A 5′-adenylated R1R adapter was then ligated to the 3′ end of the cDNA using Thermostable 5′ App DNA/RNA Ligase (New England Biolabs) for 1 h at 65 °C ...
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bioRxiv - Biochemistry 2021Quote: ... RNA integrity was assessed by performing 1% TBE agarose gel electrophoresis with samples that had been boiled for 95°C for 5 min in RNA loading dye (New England Biolabs). Genomic DNA was eliminated by incubating 2 μg of RNA with 2 U of RQ1 RNase-free DNase I (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... Full-length 265 nt 5’ UTR of SARS-CoV-2 was subcloned to replace the 5’ UTR of human CMV in the pLV-mScarlet vector using a Hifi one-step kit (Gibson Assembly, NEB). Full-length ...
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bioRxiv - Biochemistry 2020Quote: ... The substrate was designed such that the digested strand is 6 nt shorter than the undigested strand to allow 3′ fill-in by Klenow Fragment (3′-5′ exo-; NEB) with α[32P]-dCTP and cold dATP ...
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bioRxiv - Genetics 2021Quote: DNA libraries were prepared from 5 ng of total genomic DNA using the NEBNext Ultra II FS DNA Library kit for Illumina (New England Biolabs). All volumes specified in the manufacturer’s protocol were divided by four ...
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bioRxiv - Molecular Biology 2021Quote: ... Full-length 265 nt 5′ UTR of SARS-CoV-2 was subcloned to replace the 5’ UTR of human CMV in the pLV-mScarlet vector using a Hifi one-step kit (Gibson Assembly, NEB). Full-length ...
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bioRxiv - Molecular Biology 2021Quote: ... equimolar amounts of complementary oligonucleotides were annealed and end labelled with [32P]dCTP using Klenow Fragment (3′→5′ exo-) (New England Biolabs). The following oligonucleotides were used ...
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bioRxiv - Developmental Biology 2020Quote: ... and a 481-bp pph-5 3’UTR and assembled the PCR products with amplified pUC57 backbone via HiFi assembly (New England Biolabs) to generate the vector pph-5p::pph-5(cDNA)::pph-5 3’UTR (TU#2257) ...
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bioRxiv - Molecular Biology 2022Quote: The L3 DNA linker at 0.5 μM concentration (Table S1) was ligated to RNA in a 20 μl reaction using 5 U/μl of RNA Ligase Truncated K227Q (NEB) in the presence of 15% PEG8000 and 1 U/μl RNasin (Promega ...
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bioRxiv - Molecular Biology 2022Quote: ... 3’ RNA adaptor (/ 5Phos/NNNNNNNNGAUCGUCGGACUGUAGAACUCUGAAC/3InvdT/) is ligated at 5 μM concentration for 1 hours at room temperature using T4 RNA ligase (NEB), followed by 2 consecutive streptavidin bead bindings and extractions ...
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bioRxiv - Microbiology 2022Quote: ... The total RNA recovered was split into samples of 5 μg each and enriched in poly(A) transcripts using NEBNext Poly(A) mRNA magnetic isolation module (NEB) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2019Quote: ... Ncas_Int679_For 5′ GGCAAATTTGTATGAGGGATAAA and Ncas_Int679_Rev 5′ TAATTCGATTACGTTAGCTGTT) and cloned into pRS403-pGAL1-hpSC_URA3 (1) using PsiI and NaeI restriction enzymes (New England Biolabs, NEB). In addition ...
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bioRxiv - Molecular Biology 2020Quote: ... Approximately 400-800 fmol of nucleosomes purified from sucrose gradients was digested with a 5-25U titration of ExoIII enzyme (New England Biolabs) for 1 ...
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bioRxiv - Molecular Biology 2021Quote: ChIP sequencing libraries were built from immunoprecipitated DNA by first end repairing the DNA with 5 µL T4 DNA polymerase (NEB), 5 µL T4 PNK (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... CHART-enriched DNA was eluted twice in 200 μL elution buffer supplemented with 5 U/μL RNase H (New England BioLabs) at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2020Quote: ... aqueous phase containing the barcoded cDNA (~50 μL) was combined with 50 μL digestion mix containing 5 μL ExoI enzyme (NEB), 5 μL HinFI enzyme (NEB) ...
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bioRxiv - Molecular Biology 2020Quote: ... Supplementary Table 7) were ligated to 5 μg of total RNA using 10 U of T4 ssRNA Ligase 1 (NEB) in a final volume of 50 μl for 1 h at 37°C and 1X T4 of RNA Ligase Reaction Buffer (NEB ...
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bioRxiv - Molecular Biology 2020Quote: The splint ligation reaction was performed by preparing a 5 µl mastermix that consisted of 0.5 µL Hifi Taq Ligase (NEB #M0647S), 0.5 µl 10x Taq ligase buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was PCR-amplified for 25 cycles with 5’-Cy3-labelled reverse primers (IDT) and unlabeled forward primers using either Taq polymerase or Phusion high-fidelity polymerase (NEB). PCR products were separated on 40cm tall 6% polyacrylamide denaturing gels and then visualized using a Molecular Dynamics Typhoon Scanner ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA was eluted from the beads via phenol extraction or heating in water at 80oC for 5 min and ssDNA adapter [Phos]CCACGCGTGCCCTATAGTCGC[Ami] was ligated using T4 RNA ligase (NEB). Libraries were amplified and barcoded via nested and tagged PCR following the original protocol (47 ...
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bioRxiv - Genomics 2022Quote: ... The 3’ and 5’ overhangs were converted into blunt ends with NEBNext® FFPE DNA Repair Mix (NEB, Cat. M6630) and then ‘A’ base was added to 3’ blunt ends using the A-Tailing reaction (NEBNext® UltraTM II End Repair/dA-Tailing Module ...
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bioRxiv - Genomics 2020Quote: ... 5’-AATTTCTACTAAGTGTAGAT-3’ for LbCas12a) were annealed to the bottom strand and extended by Q5 High-Fidelity DNA polymerase (NEB). IVT of the dsDNA products was performed with the HiScribe T7 Quick High Yield RNA Synthesis kit (NEB ...
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bioRxiv - Genomics 2020Quote: ... and the 6-nt UMI at its 5’ end was then ligated to the 3’ end of the cDNAs by using Thermostable 5′ AppDNA/RNA Ligase (New England Biolabs) as described (35) ...
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bioRxiv - Genomics 2020Quote: PCR3 was performed by subtracting 2-3 cycles from the Ct and using 2.5 uL of a mix of distinct indexed primers from the NEBNext Dual Index Kit for Illumina (New England Biolabs) using 22.5 uL PCR2 product in a 50 uL reaction volume (Q5 UltraII mastermix with EvaGreen (Biotium) ...
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bioRxiv - Genomics 2019Quote: The circularized DNA was split into 8 50 ul rolling circle amplification (RCA) reactions (5 ul 10x Phi29 buffer (NEB), 2.5 ul 10 mM dNTPs (NEB) ...
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bioRxiv - Genomics 2019Quote: ... Beads were washed twice with SPRI wash buffer and resuspended in 50 ul of end fill-in mix (37 ul water, 5 ul 10X NEB Buffer 2 ...
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bioRxiv - Genomics 2019Quote: ... RNA was resuspended and γ-ATP was added to the 5’ end of the RNA with T4 polynucleotide kinase (NEB) for 30min at 37°C ...
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bioRxiv - Genomics 2020Quote: ... 5 μl of 10X AT buffer and 3 μl of Klenow enzyme from NEBNext® dA-Tailing Module (E6053L, NEB) was added to the sample ...
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bioRxiv - Synthetic Biology 2019Quote: ... The purified construct was then inserted at the 5’ end of the Fluc coding sequence with T4 DNA ligase (New England BioLabs). The resulting vector was termed Were-1-Fluc (Table 1).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... were coated to dry overnight at 37°C with 400 ng/well of recombinant His-tagged GT198 proteins together with 5 μg/well of purified BSA (NEB) in a volume of 50 μl ...
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bioRxiv - Microbiology 2019Quote: ... 5’ UTR reporter constructs were assembled using NEBuilder® HiFi DNA Assembly Cloning Kit (#E5520S New England Biolabs Ipswich, MA). All amplifications were carried out following the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2019Quote: ... The cap 0 standard was generated by treating the 5’-triphosphate/3’-FAM-labeled 25mer with Vaccinia virus capping enzyme (VCE, NEB) in the presence of GTP and S-adenosylmethionine (SAM) ...
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bioRxiv - Microbiology 2019Quote: ... the 11.4 kb fragments of GPV- or HIV- GPP were joined with their cognate 304 bp zip coded partial U3 inserts via Gibson Assembly in a molar ratio of 1:5 per reaction using HiFi DNA assembly mix (New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: Different CpG-containing oligonucleotides with 5’-TTAA overhangs were annealed and end-to-end ligated overnight at 16°C with T4 ligase (NEB). Oligos were ethanol-precipitated and filled-in with 1 mM biotinylated dUTP (Thermo Fisher ...
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bioRxiv - Genomics 2020Quote: ... was transcribed from a sgRNA-coding PCR product with a 5′ T7 promoter sequence using HiScibe T7 Quick High yield RNA Synthesis kit (NEB). The transcription was performed at 37 °C overnight and then purified by phenol ...
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bioRxiv - Microbiology 2019Quote: ... Reverse transcription was performed for 5 hours at 42 °C using 100 U of ProtoScript® II Reverse Transcriptase (NEB), 10 U of RNase Inhibitor (Murine ...
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bioRxiv - Molecular Biology 2019Quote: ... Fragment ends were repaired using the NEBNext End Repair Module and adenosine was added at the 3’ ends of fragments using Klenow fragment (3’ to 5’ exo minus, New England Biolabs), universal adaptors were ligated to the A-tailed DNA fragments at room temperature for 1 h with T4 DNA ligase (New England Biolabs ...
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bioRxiv - Genetics 2020Quote: ... ChIP enrichment was determined by RT-qPCR (Supplementary Table 5) prior to addition of sequencing adaptors using NEBNext Ultra II DNA Library Prep kit for Illumina (New England Biolabs). ATAC-seq was performed as previously described76 ...
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bioRxiv - Neuroscience 2019Quote: ... reverse 5’-GTATGGAATTCCTAACGTGGCTTCTTCTGCC-3’) and cloned into pAAV-CaMKIIa-hChR2(H134R)-EYFP by restriction-ligation using BamHI/EcoRI restriction enzymes (NEB) and T4 DNA ligase (NEB) ...
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bioRxiv - Plant Biology 2019Quote: ... The circularization of DNase treated total RNAs was performed by intramolecular ligation of 5’ and 3’ ends using 40U of T4 RNA ligase (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... were coated to dry overnight at 37°C with 400 ng/well of recombinant His-tagged GT198 proteins together with 5 µg/well of purified BSA (NEB) in a volume of 50 µl ...