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Citations for New England Biolabs :
3051 - 3100 of 10000+ citations for Urea Nitrogen BUN Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Radioactive probe was prepared using 40 pmol of primer 59 and 5’-labelled with 10 U of T4 Polynucleotide Kinase (New England Biolabs) and [γ32P]ATP (150 μCi) ...
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bioRxiv - Cell Biology 2022Quote: ... 10 µM Verapamil in DMSO (Spirochrome) and of 0.6 µM SNAP-Cell TMR-Star in DMSO (New England Biolabs (S9105S) for 120 mins as per manufacturers’ instructions ...
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bioRxiv - Cell Biology 2022Quote: ... genomic DNA (5-10 μg) derived from transfected ESC was digested by high concentrated HindIII-HF or high concentrated BamHI-HF (NEB). In all Southern blot analyses ...
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bioRxiv - Microbiology 2022Quote: ... and an equimolar mix of primers P5-IR2a-d primer (10 µM) in a reaction with 50 ng of adaptor ligated template and Phusion DNA polymerase (NEB) in a thermocycler with the following program 98°C 3 min ...
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bioRxiv - Genomics 2022Quote: ... the DNA was denatured and ligated to oligo ARC140 overnight at room temperature by 10 U T4 RNA ligase (New England Biolabs) in 25% PEG8000 and 1 mM CoCl3(NH3) ...
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bioRxiv - Microbiology 2022Quote: ... Institut Jacques Boy), recombinant human IL-2 (rhIL2, 10 U/ml, Miltenyi) and DNase (1 U/ml, New England Biolabs), washed and enumerated ...
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bioRxiv - Plant Biology 2022Quote: ... at 37 °C for 15 min followed immediately by 10 cycles of indexed PCR using NEBNext Ultra II Q5 DNA Polymerase (New England Biolabs) and Illumina’s PE primer set ...
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bioRxiv - Microbiology 2022Quote: ... The Guide RNAs were synthesised from Sigma and reconstituted by mixing 10 μM of the forward and reverse strands for each guide with 1 μl of 10X ligation buffer (NEB), 0.5 μl T4 polynucleotide kinase (NEB ...
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bioRxiv - Plant Biology 2022Quote: ... For quantitative PCR 90 ng of template DNA was used in 10 μl qPCR reactions with 0.25 μM primers and Luna qPCR Master Mix (NEB, USA). qPCR was performed in a LightCycler 480 (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... each 10 μL of double stranded PCR products were treated with 5 units Lambda exonuclease enzyme (New England Biolabs, NEB) at 37 °C for 45 min which is followed by 10 min at 75 °C for enzyme inactivation.
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bioRxiv - Molecular Biology 2023Quote: ... each 10 μL of double stranded PCR products were treated with 5 units Lambda exonuclease enzyme (New England Biolabs, NEB) at 37 °C for 45 min which is followed by 10 min at 75 °C for enzyme inactivation.
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bioRxiv - Molecular Biology 2023Quote: ... pre-existing SNAP-tagged histones were first quenched by incubating cells with 10 μM of the non-fluorescent SNAP reagent (SNAP-cell Block, New England Biolabs) for 30 min at 37°C followed by a 30 min wash in fresh medium and a 2-hour chase ...
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bioRxiv - Genomics 2022Quote: Add 5 μl of dNTP mix (10 mM each) and 5 μl of DNA polymerase I Klenow fragment (NEB, M0210) to the reaction system ...
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bioRxiv - Synthetic Biology 2022Quote: A double-stranded DNA scaffold template was obtained from pUCIDT-AMP-T7p_DB982 plasmid (10 ng in 50 μL reaction mixture) by PCR amplification using Phusion® DNA polymerase (NEB), T7-DB982 forward and T7-DB982 reverse primers (0.5 μM each) ...
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bioRxiv - Molecular Biology 2022Quote: 10 μg of >200nt RNA (in nuclease-free water) was decapped using 200 U yDcpS and yDcpS buffer (NEB, #M0463S) at 37°C in a thermomixer at 800rpm pulse shaking ...
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bioRxiv - Molecular Biology 2022Quote: ... IVT RNA products (2×10-3 dilution) were tested for absence of carry-over plasmid template by PCR using Taq 2X master mix (NEB). A negative PCR result would confirm the absence of carryover plasmid in the IVT product.
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bioRxiv - Molecular Biology 2024Quote: ... Each methylation reaction was thoroughly mixed on ice using 100uL of cell methylation buffer (10% volume 10x M.CviPI reaction buffer (NEB, B0227S), 300 mM sucrose ...
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bioRxiv - Genetics 2024Quote: ... containing the homology arms and guides was then ligated with the digested T2A-LexA/QF2-Hsp70-3xP3-RFP with 2.5 µl 10× T4 DNA ligase buffer (NEB B0202S) and 0.5 µl T4 DNA ligase (NEB M0202S) ...
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bioRxiv - Molecular Biology 2024Quote: ... Nicks were sealed by adding 10 μL 10x T4 DNA ligase buffer and 2 μL T4 DNA ligase (New England Biolabs) and incubating overnight at 16°C ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... gDNA (10 µg) was digested overnight at 37°C with 100 U each of the restriction endonucleases MseI (NEB, R0525L) and BglII (NEB ...
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bioRxiv - Microbiology 2024Quote: ... The coverslips are then incubated for 30 min at 37°C with DMEM supplemented with 10% SVF containing 0.12 μM SNAP-Cell TMR-Star (New England Biolabs #S9105S), rinsed twice with PBS and incubated for 45 min with Dapi (1μg/ml) ...
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bioRxiv - Genomics 2024Quote: ... dATP (3x 1.5 µL of 10 mM solutions) and 8 µL of 5 U/µl Klenow fragment of DNA polymerase I (New England Biolabs) and a 30-minute incubation at 37°C with rotation ...
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bioRxiv - Microbiology 2024Quote: ... 1 µL resuspended plaque were used for each PCR in 10 µL scale in the presence of 1 x High Fidelity PCR Master Mix (NEB) and 500 nM NudE.1 fwd and rev screening primer (Supplementary Table 2 ...
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bioRxiv - Microbiology 2024Quote: ... The radioactive probe was prepared from 40 pmoles of D072 primer (Table 1) and labeled at the 5’ end with 10 units of T4 polynucleotide kinase (New England Biolabs) and [γ-32P]-ATP (150 μCi) ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was diluted 1:10 and 3 µl of diluted cDNA was used as input for qPCR using Luna by NEB master mix for a 20 µL total reaction ...
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bioRxiv - Microbiology 2023Quote: ... 200 μl reactions were incubated with 100 μl Ni-NTA beads equilibrated in 800 μl Ni-NTA buffer A with 10 mM imidazole and 40 U murine RNase inhibitor (NEB) at 4 °C for 1 h ...
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bioRxiv - Genomics 2022Quote: ... 5 μl of cDNA was used in a 50 μl PCR reaction containing 1 μl of 10 μM PCR primer and 25 μl of 2x LongAmp Taq Master mix (NEB). PCR was performed as shown in Supplementary Protocol ...
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bioRxiv - Microbiology 2022Quote: ... The purified fragments were then 5’ phosphorylated in a 50 μL reaction containing 10 U of T4 polynucleotide kinase (NEB) and cleaned up through the Monarch PCR & DNA Cleanup spin columns (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... Samples were boiled for 10 min and 2 μL of 20 mg/ml proteinase K (New England Biolabs, Cat. #P8107S) were added ...
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bioRxiv - Molecular Biology 2023Quote: ... 2.7 µl of the sample of eluted extension products were included in a 10 µl T4 RNA ligase 2 truncated KQ reaction (1× T4 RNA ligase buffer (NEB), 2 µM IllLigBio adapter pre-adenylated using a 5’ adenylation kit (NEB) ...
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bioRxiv - Biochemistry 2023Quote: Transcription reactions were prepared at room temperature in 20 µL transcription buffer (40 µM Tris-HCl pH 8, 5 mM DTT, 10 mM MgCl2) with 2 mM NTP’s (NEB, N0450S) and 40 U/µL RNasin™ Plus (Promega ...
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bioRxiv - Microbiology 2023Quote: Lysates of HEp-2/ι1hUNGs cells mock infected or infected with wild-type HSV-1(F) at an MOI of 10 for 24 h were treated with alkaline phosphatase (CIP) (New England BioLabs) as described previously48.
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bioRxiv - Cancer Biology 2023Quote: Each pair of top and bottom oligonucleotides were phosphorylated and annealed by incubating 10 µM of each with 1 × T4 DNA ligase buffer (New England Biolabs), 5U T4 polynucleotide kinase (New England Biolabs ...
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bioRxiv - Cancer Biology 2023Quote: ... lysate volume equivalent to 20-40 µg total protein was first denatured at 100°C for 10 min in 1X glycoprotein denaturation buffer (NEB). Endo H digestion was performed at 37°C for 1h in the presence of 1X GlycoBuffer 3 and 1 µL of Endo H enzyme (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... Nanopore sequencing adapters were first ligated to Cas9 cut sites by incubating 10 μl of NEBNext Quick T4 DNA Ligase (NEB), 20 μl ligation buffer (ONT) ...
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bioRxiv - Biochemistry 2023Quote: ... Bleo-treated WT-PNKP-FLAG and K226R-PNKP-FLAG cells using 10 μg of custom-made rabbit polyclonal anti-AcK226 Abs (Ez Biolabs). All other subsequent steps and buffers used for IP were the same as described earlier (17,18) ...
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bioRxiv - Cancer Biology 2023Quote: ... 1:100 10 mg/mL BSA in distilled water) with 0.75 µL (150 U) micrococcal nuclease enzyme solution (1:10 micrococcal nuclease (NEB, USA) in micrococcal nuclease reaction buffer) ...
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bioRxiv - Cell Biology 2023Quote: ... To remove RNA secondary structure and anneal the mRNA capture primer 1 μL of tagged random hexamer (100 μM) and 0.5 μL of 10 mM dNTPs (dNTP solution set NEB - N0446S) were added to the sample ...
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bioRxiv - Biophysics 2023Quote: ... we mixed the digested constructs and handles in a 1:10 molar ratio and ligated them together using T4 DNA ligase in T4 ligase buffer (New England Biolabs) at 16°C overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... in 100 µL MNase reaction mix (87 µL ddH2O, 10 µL 10x MNase buffer, NEB, 1 µL 100x BSA, 2 µL 2000 U/µL MNase, NEB). Digests were centrifuged (5 min ...
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bioRxiv - Molecular Biology 2023Quote: ... pre-existing SNAP-tagged histones were quenched during 30 min with 10 µM of the non-fluorescent reagent SNAP-cell Block (New England Biolabs), followed by a 30-min wash in fresh medium and a 2-h chase ...
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bioRxiv - Neuroscience 2023Quote: ... and the samples were resuspended in cold Nuclei suspension buffer (5 mL PBS, 50 μL NEB BSA (10 mg/mL), and 25 μL RNase inhibitor (40 U/μL)) ...
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bioRxiv - Molecular Biology 2023Quote: ... was also reverse-transcribed and Illumina sequencing library prep was followed by 8–10 cycles of polymerase chain reaction (PCR) using High Fidelity Phusion (New England Biolabs). All the libraries were barcoded in the PCR step ...
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bioRxiv - Systems Biology 2023Quote: ... The glutathione or amylose agarose was then washed 5-10 times to remove unbound proteins before boiling and analysis by SDS-PAGE WB using anti-MBP (NEB) and anti-GST (abcam ...
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bioRxiv - Developmental Biology 2023Quote: ... 3 µl of the purified assembly is incubated with 50 µl of NEB 10-β-competent E.coli cells (NEB, C3019H) for 30 min at 4 ℃ ...
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bioRxiv - Molecular Biology 2023Quote: Equilibration buffer (GF150 buffer supplemented with 0.1 mM Mg-ATP and 10% glycerol) was used to equilibrate 20 μL of SNAP-Capture Magnetic Beads (New England Biolabs) slurry in 1.5 mL Protein Lo Bind Tubes (Eppendorf) ...
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bioRxiv - Microbiology 2023Quote: ... at a final volume of 20 μL with 10 μL of Luna Universal qPCR Master Mix (New England Biolabs, Germany), which uses SYBR Green chemistry ...
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bioRxiv - Biochemistry 2023Quote: ... DNA pellets were suspended in 50 μl of H2O and 10 μg of DNA was digested by EcoRI restriction endonuclease (NEB) at 37 °C for 1 h ...
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bioRxiv - Immunology 2022Quote: ... 2 µl preramp PCR product was mixed with 48 µl tagging PCR mix (10 µl 5x phusion HF buffer (NEB); 1 µl Phusion Hot Start II DNA Polymerase (2U/ µl ...
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bioRxiv - Cancer Biology 2023Quote: The commercially synthesized oligo pool was diluted 1:10 in water and amplified using the Phusion HotStart Flex polymerase (NEB) according to the manufacturer’s protocol with the Array_F and Array_R primers (Supplementary Table 1) ...