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Citations for New England Biolabs :
2901 - 2950 of 10000+ citations for Urea Nitrogen BUN Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... The synthesized sgRNAs were purified using a Monarch RNA purification kit (NEB), and quality and quantity were assessed using UV-Vis spectroscopy on a Synergy H1 plate reader (Biotek) ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was transcribed using HiScribe T7 High Yield RNA Synthesis Kit (NEB) according to manufacturer’s directions ...
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bioRxiv - Microbiology 2024Quote: ... PCR-amplified DNA fragments were assembled by Gibson assembly kit (NEBuilder, NEB) as per manufacturer’s protocol and introduced into DH5α E ...
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bioRxiv - Molecular Biology 2024Quote: ... In-vitro transcription (HiScribe T7 High Yield RNA Synthesis Kit E2040L, NEB) from the DNA was then carried out ...
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bioRxiv - Molecular Biology 2024Quote: ... rRNA depletion was conducted using NEBNext rRNA depletion kit v2 (NEB, #E7400L) followed by end-repair using T4 PNK after purification of the rRNA depleted samples using Oligo Clean & Concentrator Kits (Zymo research ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA samples were additionally processed with the Monarch RNA Cleanup Kit (NEB), analyzed for integrity with a TapeStation (Agilent) ...
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bioRxiv - Cell Biology 2024Quote: ... we use the Monarch® Genomic DNA Purification Kit (New England Biolabs) and follow the manufacturer recommendation ...
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bioRxiv - Cancer Biology 2024Quote: ... Reverse transcription was performed with the LunaScript RT SuperMix Kit (#E3010, NEB) using the manufacturer’s protocol on a SimpliAmp thermal cycler (Applied Biosystems ...
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bioRxiv - Cancer Biology 2024Quote: ... Plasmids were recovered using Monarch Plasmid Miniprep Kit (New England Biolabs #T1010S) or E.Z.N.A ...
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bioRxiv - Cell Biology 2024Quote: Monarch® Genomic DNA Purification Kit (New England Biolabs, cat. no. T3010S)
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bioRxiv - Cell Biology 2024Quote: Monarch® PCR & DNA Cleanup Kit (New England Biolabs, cat. no. T1030S)
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bioRxiv - Microbiology 2024Quote: ... Digested products were cleaned using Monarch® PCR & DNA Cleanup Kit (NEB) and incubated with T4 DNA ligase (NEB ...
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bioRxiv - Microbiology 2024Quote: ... and cleaned with the Monarch RNA Cleanup Kit (NEB, Ipswich, MA, USA). Ribosomal 23S and 16S samples were prepared by amplifying the appropriate sequences from genomic E ...
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bioRxiv - Biochemistry 2024Quote: ... Transcription was performed using T7 HiScribe RNA synthesis kit (New England Biolabs). Generated RNA was subjected to DNase treatment (TURBODNase ...
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bioRxiv - Cancer Biology 2024Quote: ... using the NEBNext® Ultra TM II DNA Library Prep Kit (NEB E7645S/L ...
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bioRxiv - Microbiology 2024Quote: ... followed by enzyme removal via Monarch RNA Cleanup Kit (New England Biolabs). cDNA was generated from 1 µg RNA using LunaScript RT SuperMix Kit (New England Biolabs) ...
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bioRxiv - Molecular Biology 2024Quote: ... Then we used ProtoScript® II First Strand cDNA Synthesis Kit (NEB) and 3′-RACE CDS primer (ClonTech ...
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bioRxiv - Microbiology 2024Quote: Total RNA samples were treated with a NEBNext rRNA depletion kit (NEB) to remove rRNA ...
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bioRxiv - Molecular Biology 2024Quote: ... and purified with the Monarch Total RNA Miniprep Kit (New England Biolabs). Alkylated RNA was purified with the Monarch RNA Cleanup Kit (New England Biolabs) ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized using LunaScript RT SuperMix Kit (New England Biolabs, USA). SYBR green assay was used to determine HBV pregenomic expression (Luna Universal qPCR Master Mix ...
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bioRxiv - Molecular Biology 2024Quote: ... then purified using the Monarch RNA Cleanup Kit (T2040L, New England Biolabs). Quality and size of the produced RNAs were assessed using an Agilent Fragment Analyzer.
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bioRxiv - Genetics 2024Quote: All plasmids were constructed using Hifi DNA Assembly Cloning Kit (NEB, USA) and then midipreped with ZymoPure Midiprep Kit (Zymo Research ...
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: Total RNA was extracted with Monarch Total RNA Miniprep Kit (NEB #T2010S). The integrity of the RNA was determined using the TapeStation 4150 (High Sensitivity RNA kit ...
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: ... The RNA samples were cleaned with Monarch RNA Cleanup Kit (NEB #T2030) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... mRNAs were purified using the Monarch RNA Cleanup Kit (New England BioLabs) and eluted in RNAse-free water ...
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bioRxiv - Cell Biology 2024Quote: ... and NEBNext Ultra TMII Directional RNA Library Prep Kit (New England Biolabs). Libraries were sequenced with an Illumina NovaSeq 6000 to obtain 150 bp ×2 paired-end reads ...
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bioRxiv - Microbiology 2024Quote: ... and HiFi DNA Assembly kit were purchased from New England Biolabs (NEB). All bacterial strains were PCR or sequence verified before use ...
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bioRxiv - Microbiology 2024Quote: ... using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs, E0554S). Homology-directed repair templates were PCR amplified using the CloneAmp™ HiFi PCR Premix (Takara Bio ...
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bioRxiv - Microbiology 2024Quote: ... The Primer-free LunaScript® RT Master Mix Kit (New England Biolabs) was used for first strand cDNA synthesis using a gene-specific primer targeting the mCherry gene in the genomic vRNA (primer sequence 5’-CGCTTCAAGGTGCACATGG-3’) ...
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bioRxiv - Genomics 2024Quote: ... Genomic DNA was extracted using the Monarch gDNA Purification Kit (NEB T3010S) following the standard protocol but with elution using 175 µL 25 mM K3BO3 at pH 7.0.
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bioRxiv - Cell Biology 2024Quote: ... and plasmids were purified using a Monarch Plasmid Miniprep Kit (NEB #T1010). Successful mutagenesis was verified through DNA sequencing (Plasmidsaurus).
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bioRxiv - Cell Biology 2024Quote: ... and plasmids were purified using a Monarch Plasmid Miniprep Kit (NEB #T1010). Constructs were then verified by restriction digest using SacI-HF (NEB #R3156 ...
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bioRxiv - Developmental Biology 2024Quote: ... Plasmid DNA was extracted using the Monarch Miniprep Kit (NEB, Cat. #T1010L) and sent to sequencing to identify mutations.
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bioRxiv - Developmental Biology 2024Quote: ... PCR products were purified using the Monarch PCR Cleanup Kit (NEB, T1030L) and digested with the TfiI restriction enzyme (NEB ...
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bioRxiv - Developmental Biology 2024Quote: ... using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs, Ipswich, MA).
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bioRxiv - Developmental Biology 2024Quote: ... using the NEBuilder HiFi DNA Assembly Kit (New England BioLabs, Ipswich, MA) and recombined into a UASp-attB destination vector (gift of F ...
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bioRxiv - Developmental Biology 2024Quote: ... using the NEBuilder HiFi DNA Assembly Kit (New England BioLabs, Ipswich, MA).
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bioRxiv - Developmental Biology 2024Quote: ... PCR products were purified using the Monarch PCR Cleanup Kit (NEB, T1030L). PCR products were A-tailed ...
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bioRxiv - Cell Biology 2024Quote: ... The RNA was then made using a T7 Highscribe kit (NEB #E2040S), treated with DNase I (NEB #M0303L ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries were prepared with the NEBNext Ultra II Directional RNA Kit (NEB). Samples were equimolarly pooled and sequenced on a NextSeq550 system (Illumina ...
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bioRxiv - Cell Biology 2024Quote: ... The insertion was done using the Q5 Site-Directed Mutagenesis Kit (NEB) and design of respective primers using the NEBaseChanger online tool (http://nebasechanger.neb.com/).
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bioRxiv - Plant Biology 2020Quote: ... 2 µg of RNA for each sample was treated with DNase I at 37°C for 10 minutes (New England Biolabs) and 0.5 µg was used for reverse transcription with the GoScript Reverse Transcription Mix ...
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bioRxiv - Microbiology 2020Quote: ... Illumina sample-specific barcodes and sequencing adapters were added in a final PCR reaction consisting of (per sample) 10 μl 2x Hot Start Taq master mix (New England BioLabs), 2 μl each of Nextera N7 and S5 barcoding primers (Illumina) ...
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bioRxiv - Biophysics 2020Quote: ... The PCR was typically performed in 10 x 50 µL volume by mixing in PCR tubes Phusion HF buffer (1x, New England Biolabs), plasmid template (0.02 ng/µL) ...
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bioRxiv - Molecular Biology 2019Quote: ... up to 500 ng of gDNA was incubated in 10 µL H2O with 1x CutSmart buffer and 0.5 U rSAP (New England BioLabs #M0371L) for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Quantification of mRNA level was performed in a 20 μl mixture consisting of 10 μl Q5 High-Fidelity 2X Master Mix (NEB), 0.2 μl RT-PCR product ...
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bioRxiv - Developmental Biology 2021Quote: ... Following the adaptor ligation each sample was mixed with 20 μl of a 2x DpnII Digestion Buffer and 10 units (1 μl) of DpnII restriction enzyme (New England Biolabs) mastermix and were incubated for 3 hours at 37 °C ...
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bioRxiv - Genetics 2021Quote: ... which was followed by 37°C for 30 min with addition of 50ul 10% Triton-X 100 to quench SDS and 37°C overnight with addition of 40ul AluI (R0137L, NEB) total ...
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bioRxiv - Molecular Biology 2021Quote: ... 300 μM of complementary primers containing 20 bp overlap - target sequence - and 4 bp of homology to the expression vector were hybridized in a reaction containing 10 μL of 10x T4-ligase buffer (New England Biolabs). The mixture was submitted to 70 decreasing cycles of a minute each - touchdown - from 95 to 25°C and then connected to the previously linearized vector through a reaction containing 15 ng of the previous hybridization ...
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bioRxiv - Developmental Biology 2020Quote: ... TA overhangs were added by incubating the insert for 10 min at 72 °C with 50mM DNTPs and Taq polymerase (NEB), in order to perform TA cloning into a PCR4 TOPO vector (ThermoFisher) ...