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Citations for New England Biolabs :
251 - 300 of 2731 citations for Who Coplanar & Mono Ortho Pcb + Pcb 170 & Pcb 180 13C12 99% 20 Ng Ml In N Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... was carried out (25 ng linearized vector, 10 ng purified insert, 10 µL 2 x Gibson Assembly Master Mix (New England BioLabs) and up to 20 µL H2O were mixed and incubated at 50°C for 1 hour) ...
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bioRxiv - Genomics 2024Quote: ... The purified scaffold insert (2 ng) was ligated with the digested intermediate plasmid library vector (200 ng) using T4 DNA Ligase (NEB) at room temperature for 45 min ...
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bioRxiv - Microbiology 2024Quote: ... 25 ng of linearised plasmids were ligated with 75 ng of purified VH and VL by Gibson Assembly (New England BioLabs). The reaction was performed in a final volume of 5 μL ...
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bioRxiv - Microbiology 2024Quote: ... The scFv DNA (256 ng) was ligated into the phagemid vector (400 ng) using the T4 Ligase Kit (New England BioLabs) in 25 × 40 μl reactions and incubated at 16°C overnight ...
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bioRxiv - Genetics 2021Quote: ... 20 units NheI (#R0131S; NEB), 20 units AvrII ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 20 units of TdT (NEB) and the provided TdT buffer ...
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bioRxiv - Biochemistry 2020Quote: ... Exonuclease I (20 U, NEB) were added to digest cDNA and oligonucleotide sequences ...
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bioRxiv - Genetics 2019Quote: ... Cas9 protein (20 µM, NEB) was mixed in a 1:1 ratio with the gRNA complex and incubated for 5-10 minutes at room temperate to produce the Cas9:gRNA RNP complex at a final concentration of 10 µM ...
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bioRxiv - Microbiology 2020Quote: ... Exonuclease I (20 U, NEB) was added to each PCR reaction and incubated at 37 °C for 15 minutes ...
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bioRxiv - Genomics 2022Quote: ... 20 μl Cutsmart buffer (NEB), 3 μl HinP1I (NEB R0124S) ...
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bioRxiv - Microbiology 2022Quote: ... 20 U BsaI-HFv2 (NEB) and 20 U T4 ligase (NEB) ...
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bioRxiv - Systems Biology 2023Quote: ... 20 units NheI (#R0131S; NEB), 20 units AvrII ...
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bioRxiv - Developmental Biology 2023Quote: ... supplemented with 20% PEG8000 (NEB). Ligated miRNA-adapter fragments were gel-purified and eluted in 0.3 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... 250 ng of plasmids pBR322 or pUC19 (NEB) were combined with 15 µl of protein fractions ...
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bioRxiv - Immunology 2020Quote: ... 50 ng/µL lambda DNA (New England Biolabs), 5 pmol/µL methylation or nonmethylation-specific probe ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 ng/μl ET SSB (New England Biolabs), 3 mM MnCl2 (Fisher Scientific) ...
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bioRxiv - Genetics 2023Quote: ... 0.5 ng of unmethylated phage-λ DNA (NEB) was spiked into each DNA sample prior to shearing to allow assessment of the efficiency of the bisulfite-conversion reaction ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: For analysis of protein N-glycosylation PNGase F (P0704S, New England Biolabs) was used and the assay was performed according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: Removal of N-linked glycans and glycosaminoglycans (GAGs) using PNGaseF (NEB #P0704), heparinase II (NEB #P0736) ...
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bioRxiv - Biochemistry 2021Quote: Peptide-N-glycosidase F (PNGase F) (New England Biolabs Inc., Cat. P0704S) was used for complete removal of N-linked oligosaccharides ...
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bioRxiv - Molecular Biology 2022Quote: ... and α-N-acetyl-galactosaminidase (New England Biolabs, 10 U/μg protein). Removal of sialic acids was performed using the α2-3,6,8 neuraminidase (New England Biolabs ...
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bioRxiv - Biochemistry 2021Quote: ... To release N-glycans 2 μl of PNGase F (New England Biolabs) was added and the samples were incubated for 18 h in 37°C ...
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bioRxiv - Microbiology 2023Quote: The N-glycans of ACE2 were removed by PNGase F glycosidase (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... of the SARS-CoV-2 Positive Control (N gene) plasmid (NEB N2117). Sanger sequencing confirmed successful mutagenesis ...
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bioRxiv - Evolutionary Biology 2022Quote: ... a total of 575 embryos were injected with a mixture containing 300 ng/μL of sgRNA (one guide) and 600 ng/μL of Cas9 protein (NEB, M0641) while for Dll ...
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bioRxiv - Genomics 2021Quote: ... 510 ng (6 µl at 85 ng/µl) of DNA was digested using endonucleases EcoRI and MseI (New England BioLabs, Inc.) after which barcoded (EcoRI cut site ...
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bioRxiv - Bioengineering 2023Quote: ... and ligated with the digested pSubMAAP vector at a 100 ng : 37.5 ng vector : insert ratio using T4 DNA ligase (New England Biolabs, Ipswich, MA). The ligation reactions were desalted for 40 minutes using a membrane on molecular-grade H20 and electroporated into DH10B electrocompetent cells ...
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bioRxiv - Genomics 2023Quote: ... The gel-purified DGP-4 fragments (200 ng) and p200 vector (200 ng) were mixed and ligated in a 50 µL T4 ligation reaction (NEB, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Recombination was performed by incubating the linearized vector (330 ng) with the amplicons pool (50 ng) and the NEBuilder HiFi DNA Assembly (NEB #E2621) 50°C for 15 minutes to one hour ...
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bioRxiv - Genetics 2024Quote: ... 400 nM of each of the flanking primers PS1057-NGS-F and PS1057-NGS-R (S2 File) and 0.1 units/µl of LongAmp Taq DNA polymerase (NEB Cat. # M0323L). In a second amplification step ...
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bioRxiv - Biophysics 2019Quote: ... NG-Stop was created using the same inverse PCR product as NG-Scarlet and NG-Cherry via blunt end ligation using NEBs KLD Mix (New England Biolabs; Ipswich, MA). The mNeonGreen gene was obtained from the pmNeonGreen-NT plasmid (Allele Biotehcnology ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were constructed from 300 ng (liver) or 200 ng (hypothalamus) total RNA (NEBNext Multiplex Small RNA library prep set for Illumina, New England Biolabs, NEB-E7560S) and the small RNA fraction was sequenced on the NextSeq 500 System (Illumina ...
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bioRxiv - Evolutionary Biology 2023Quote: ... before being incubated in 5% normal goat serum dissolved in PBS-TX (NGS-PBS-TX; NGS; New England BioLabs, Hitchin, Hertfordshire, UK) for a minimum of one hour at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... 20 units PNK enzyme (NEB, M0201S), 30 μCi γ-32P-ATP ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 U DpnI (New England Biolabs), and completed the volume to 60 μl with PCR grade water ...
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bioRxiv - Biochemistry 2020Quote: ... and 20 units RNase inhibitor (NEB) in 75 mM KCl ...
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bioRxiv - Cancer Biology 2021Quote: ... G7 Reaction buffer (20 μL, NEB), deionized water (60 μL ...
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bioRxiv - Cancer Biology 2021Quote: ... 10% NP-40 (20 μL, NEB), G7 Reaction buffer (20 μL ...
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bioRxiv - Biophysics 2020Quote: ... 20 μl of 10x ATP (NEB), 154 ul water ...
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bioRxiv - Molecular Biology 2019Quote: ... 20 U of Exonuclease I (NEB) was added to ~5 ug of DNA to digest free overhangs at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... and 20 U EcoRV-HF (NEB) in the same reaction mixture ...
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bioRxiv - Biophysics 2019Quote: ... coli RNAP holoenzyme (20 nM, NEB), ATP ...
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bioRxiv - Genomics 2019Quote: ... then 20 Units Bfa I (NEB) at 37°C for 20 minutes ...
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bioRxiv - Genomics 2019Quote: ... and 20 Units CviA II (NEB) at 25°C for 20 minutes ...
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bioRxiv - Genomics 2020Quote: ... 20 μg BSA (NEB, Ipswich, MA) using 0.2 μg of APOBEC3A ...
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bioRxiv - Genomics 2019Quote: ... treated with PNK (20 U, NEB) for 20 min at 37°C and then re-annealed with 8 μM RT-primer (70°C ...
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bioRxiv - Microbiology 2023Quote: ... and 20 U DNase I (NEB) were added ...
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bioRxiv - Microbiology 2022Quote: ... and 20 U T4 ligase (NEB), and run in a thermal cycler with an initial 1 hr 37 C step followed by ...
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bioRxiv - Microbiology 2023Quote: ... and 20 units RNase inhibitor (NEB) in 75 mM KCl ...
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bioRxiv - Biochemistry 2023Quote: ... 20 µg factor Xa protease (NEB) and 5 mM CaCl2 with inversion at 10 °C overnight ...