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Citations for New England Biolabs :
451 - 500 of 2731 citations for Who Coplanar & Mono Ortho Pcb + Pcb 170 & Pcb 180 13C12 99% 20 Ng Ml In N Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... 20 μls (200 units) of proteinase K (NEB, USA) and 20 μl of 20% SDS was added and the tube was incubated at 65 °C for o/n ...
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bioRxiv - Neuroscience 2022Quote: ... 15-20 µL of amylose beads (New England Biolabs) were mixed with (MBP)2-WRC (bait ...
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bioRxiv - Molecular Biology 2022Quote: ... then incubated with 20 U of DNaseI (NEB # M0303S) at 37°C for 15 min ...
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Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: ... 20 µl phi29 DNA Polymerase (10 U/µl, NEB) and incubated for 12 h at 30 °C ...
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bioRxiv - Biophysics 2023Quote: ... and 20 U CpG methyltransferase M.SssI (New England BioLabs) (20 μL total volume ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µL of 50% (v/v) amylose resin (NEB) was added to each sample ...
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bioRxiv - Biochemistry 2023Quote: ... 20 μL of 500 mM PNPP (New England Biolabs) was then added to the 190 μL protein mixture (per well ...
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bioRxiv - Biochemistry 2022Quote: ... 20 μM (nt) ΦX174 Virion DNA (New England Biolabs) was incubated in 1X RecA buffer (25 mM Tris-acetate (80% cation ...
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bioRxiv - Cancer Biology 2023Quote: ... then 1 μl 20 U/μl SfiI (NEB R0123S) was added and incubation continued overnight at 50°C ...
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bioRxiv - Cell Biology 2023Quote: ... 20 mM vanadyl ribonuclease complex (New England Biolabs, S1402S), and 20 μM β-mercaptoethanol ...
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bioRxiv - Cell Biology 2024Quote: ... and 20 U/μL terminal transferase (NEB, no. M0315S). After incubation at 37°C for 30 min and termination at 70°C for 10 min ...
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bioRxiv - Molecular Biology 2020Quote: ... padlock probe ligation was performed overnight O/N at 25°C using the SplintR ligase (NEB, M0375) at a final concentration of 0.5 Units/μl ...
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bioRxiv - Biochemistry 2020Quote: 1 μg of N protein was dissolved in 36 μL H218O and 2 μL 10x glycobuffer (NEB). To this solution 2 μL PNGase F was added and the reaction mixture was incubated at 37 °C for 16 h ...
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STARCH SYNTHASE 4 is required for normal starch granule initiation in amyloplasts of wheat endospermbioRxiv - Plant Biology 2021Quote: ... in frame with the N-terminal His6-tag using the Gibson assembly master mix (New England Biolabs) for TaSS4-1B ...
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bioRxiv - Microbiology 2020Quote: ... and 2 μL of endo-β-N-acetylglucosaminidase-H (Endo-H, EC 3.2.1.96) (New England Biolabs™) and incubated in the thermomixer at 37 °C for one h ...
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bioRxiv - Microbiology 2019Quote: ... the product was cloned into the pET1-5b N-terminal 6×His expression plasmid (New England Biolabs).
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bioRxiv - Cell Biology 2020Quote: ... then deglycosylated with 2000 U of Peptide-N-Glycosidase F (PNGase F) (New England BioLabs, MA, USA). All samples were incubated for 16 hours at 37°C ...
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bioRxiv - Cancer Biology 2019Quote: ... N-linked glycans released from glycoproteins using Peptide:N-glycosidase F (PNGase F, New England Biolabs, Ipswich, MA) were mixed with 2,5-dihydroxybenzoic acid (DHB ...
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bioRxiv - Biochemistry 2021Quote: The full-length PARP1 gene was purchased from GE Healthcare and subcloned into a pACEBac1 plasmid bearing an N-terminal 6xHis-tag via a Gibson Assembly (NEB). The PARP2 expression plasmid (C-terminal FLAG-6xHis-tag ...
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bioRxiv - Molecular Biology 2022Quote: ... with a cleavable Glutathione-S-Transferase (GST) tag at the N-terminus) and pMALTMc5X (New England Biolabs, USA ...
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bioRxiv - Neuroscience 2023Quote: ... Generation of Adgrd1 N-terminal mutations was carried out using Q5 site directed mutagenesis kit (NE Biolabs) using manufacturers protocol ...
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bioRxiv - Cell Biology 2023Quote: cDNA encoding recombinant MUC17(7TR) with N-terminal 3xFlag tag was generated using Gibson Assembly (E2611S, NEB) following the manufactures protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Cascade complexes were purified via the N-terminal maltose binding protein (MBP) tag using amylose beads (NEB) and eluted with lysis buffer containing 10 mM maltose ...
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bioRxiv - Genetics 2023Quote: We performed n=30 PCR1 reactions per sample using Q5 High Fidelity 2X Master Mix (NEB #M0429S) with 10 ug of genomic DNA to maintain ≥1000X representation ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were resuspended in spheroblast buffer (1.2 M sorbitol, 0.1 M potassium phosphate, 20 mM vanadyl ribonuclease complex [NEB S1402S], 20 μM beta-mercaptoethanol) and digested with 0.002% 100T zymolyase (US Biological Z1005 ...
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bioRxiv - Developmental Biology 2021Quote: ... 200 ng of PCR products in 1X NEB buffer 2 (New England Biolabs) were hybridized under the following conditions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 100 ng of DNA was digested with PstI (New England Biolabs, Ipswitch, USA). The digested DNA fragments were ligated to a barcode adapter-common adapter system (0.045 pmol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The ladder mix consisted of 500 ng ssRNA ladder (50-1000nt, NEB#N0364S), and 5-10 ng of each 21 & 26-nt RNA oligo loaded per lane ...
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bioRxiv - Developmental Biology 2020Quote: ... Cas9 protein (300 ng/μL; EnGen® Spy Cas9 NLS, New England Biolabs) and gRNAs (100 ng/μl ...
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bioRxiv - Molecular Biology 2021Quote: ... NGS libraries were made using NEBNext Ultra II DNA Library Prep kit (NEB). DNA sequencing was performed on Illumina MiniSeq with 150+150 bp paired-end protocol.
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bioRxiv - Developmental Biology 2019Quote: ... Purified total RNA (2,000 ng) from each sample was treated with DNasel (NEB) and 12 μl was used to synthesize cDNA using the Protoscript II First Strand cDNA Synthesis Kit (NEB ...
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bioRxiv - Genomics 2021Quote: - Extreme Thermostable Single-Stranded DNA Binding Protein (ET SSB, 500 ng/μL, NEB) was tested in 4 different amounts ...
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bioRxiv - Bioengineering 2021Quote: ... 10 ng of purified PCR products were incubated with I-SceI endonuclease (NEB) according to manufacturer’s instruction ...
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bioRxiv - Neuroscience 2019Quote: ... 100 ng of genomic DNA was digested with 10 units of HhaI (NEB) and 2 units of HaeIII (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... 50 ng of the PCR product was ligated with T4 DNA ligase (NEB) in a final volume of 10 μL overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: The final microinjection mix contained 322 ng/μL spCas9 protein (New England Biolabs) with 40 ng/μL of each sgRNA and 127 ng/μL of the ssDNA repair template ...
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bioRxiv - Molecular Biology 2021Quote: ... The ladder mix consisted of 500 ng ssRNA ladder (50-1000nt, NEB#N0364S), and 5-10 ng of each 21 & 26-nt RNA oligo loaded per lane ...
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bioRxiv - Molecular Biology 2022Quote: ... NGS libraries were prepared using NEBNext Ultra II DNA Library Prep kit (NEB). DNA sequencing was performed on Illumina MiSeq with the 250+250 bp paired-end protocol ...
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bioRxiv - Microbiology 2022Quote: ... and 300 ng bovine serum albumin (BSA; New England BioLabs Inc. Ipswitch, MA), to which nuclease-free H2O was added to reach a volume of 25 µl ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 ng of full-length cDNA was amplified with LongAmp master mix (NEB) and TSO (5’-NNNAAGCAGTGGTATCAACGCAGAG-3’ ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 ng of DNA was used per qPCR reaction (qPCR Master Mix, NEB). PCR was performed using an Applied Biosystems 7500 instrument (software version 2.3) ...
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bioRxiv - Cell Biology 2019Quote: ... 25 ng of the PCR product was ligated with T4 DNA Ligase (NEB) in a final volume of 10 μL for 1h at RT ...
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bioRxiv - Synthetic Biology 2019Quote: ... and 100-200 ng of DNA per sample was digested with MmeI (NEB) for 1 hour in a reaction volume of 40 μL ...
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bioRxiv - Microbiology 2021Quote: ... a mass of 200 ng of the amplicons was digested using SapI (NEB), followed by a single elongation step PCR by adding an equal volume of previously prepared Phusion polymerase PCR reaction mix at 2X final concentration (Phusion™ High-Fidelity DNA Polymerase ...
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bioRxiv - Plant Biology 2022Quote: ... 87 ng template DNA and 1-units Phusion DNA Polymerase (NEB, Evry, France). The PCR product was cloned using the hot fusion method (Fu et al. ...
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bioRxiv - Genomics 2020Quote: ... 200 ng of each genomic DNA was subjected to exoV (New England Biolabs) digestion as described by (Shoura ...
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bioRxiv - Molecular Biology 2022Quote: ... 4.2 ng/μl extreme thermostable single-stranded DNA binding protein (New England Biolabs) and 0.02 U/μl SuperFi DNA polymerase (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2022Quote: ... Of each entry plasmid 100 ng was combined with 1× Cutsmart buffer (NEB), 1 mM ATP ...
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bioRxiv - Microbiology 2023Quote: ... Cap17 was also incubated with 50 ng/μL of lambda DNA (NEB #N3011S) or control RNA (Ambion MicrobExpress ...
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bioRxiv - Genetics 2023Quote: ... and 250 ng was bisulfite-treated using the EpiMark Bisulfite Conversion Kit (NEB) with case and control samples randomly distributed across arrays ...