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Citations for New England Biolabs :
251 - 300 of 930 citations for Tripartite Motif Containing 22 TRIM22 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... and then mounted using 10 μl ProLong Gold Antifade Reagent containing DAPI (9071S, New England Biolabs). The slides were imaged when the mounting medium was fully cured >12 h.
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bioRxiv - Evolutionary Biology 2020Quote: ... A DNA fragment containing the aanat2 target fragment was digested with BbvI (New England Biolabs Inc.). The rorca DNA fragment was digested with Cac8I (New England Biolabs) ...
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bioRxiv - Biochemistry 2019Quote: Activating adaptors containing amino-terminal HaloTags were expressed in BL-21[DE3] cells (New England Biolabs) at OD 0.4-0.6 with 0.1 mM IPTG for 16 hr at 18°C ...
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bioRxiv - Systems Biology 2019Quote: ... 5 mM CaCl2 and 25% (v/v) glycerol)) containing MNase ((micrococcal nuclease (M0247S, New England Biolabs)) and supplemented with protease inhibitor ...
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bioRxiv - Molecular Biology 2019Quote: ... 10 mM CaCl2 and 1x PIC) containing 2U/μl of micrococcal nuclease (New England BioLabs, M0247S) and incubating on ice for 20 min and then 37°C for 10 min ...
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bioRxiv - Genomics 2019Quote: ... Transposed DNA was amplified in a solution containing 1X NEBNextHigh Fidelity PCR Master Mix (NEB #M0541L), 0.5 μM of Ad1_noMX universal primer ...
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bioRxiv - Microbiology 2019Quote: ... adjacent primers containing the mutation of interest were 5′-phosphorylated using T4 polynucleotide kinase (NEB, M0201S) using the buffer from the T4 DNA ligase ...
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bioRxiv - Biochemistry 2020Quote: ... excised bands containing 20 μg of gp145 were incubated with 5000 units of PNGase F (NEB) for 24 hrs at 37°C [31] ...
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bioRxiv - Biochemistry 2021Quote: ... The reactions were quenched with native loading dye containing EDTA (New England Biolabs, Cat. no. #B7025S). Samples were loaded on a 0.7% agarose gel and run at 120 V in 45□mM (0.5× ...
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bioRxiv - Molecular Biology 2021Quote: ... The 20 µl reaction mix containing 80 ng of single-stranded M13mp18 DNA (New England Biolabs), 10 mM Tris·HCl (pH 8.0) ...
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bioRxiv - Molecular Biology 2020Quote: ... 2.5 μl of enzyme mixture containing 0.01 U Bst 2.0 DNA polymerase (New England Biolabs, M0537S), 0.5 U SplintR ligase (New England Biolabs ...
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bioRxiv - Cell Biology 2021Quote: Activating adaptors containing amino-terminal HaloTags were expressed in BL-21[DE3] cells (New England Biolabs) at OD 0.4-0.6 with 0.1 mM IPTG for 16 hr at 18°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Annealed oligonucleotides containing the homology region were phosphorylated with T4 polynucleotide kinase (New England Biolabs, M201) and then ligated into the Bbs1 site of the vectors pX335 and pKN7 (Appendix Tables S1 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... with each containing five units of Taq Plus and polymerase buffer (New England, BioLabs®, UK), 2 mM MgCl2 ...
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bioRxiv - Pathology 2022Quote: ... and 1 mM DTT containing [3H] SAM (25:1 molar ratio of SAM(NEB, Cat#B9003S) to [methyl-3H]-SAM (PerkinElmer ...
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bioRxiv - Neuroscience 2022Quote: ... Gels were then placed in digestion solution containing proteinase K (8U/mL, New England BioLabs, P8107S) and digested for 4 hours at room temperature.
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bioRxiv - Molecular Biology 2019Quote: ... a master mix was prepared containing 2 µl 10x T4 polynucleotide kinase buffer without ATP (NEB) and 1 µl murine RNase inhibitor per sample and 3 µl were added to each sample together with 2 µl truncated T4 polynucleotide kinase (#M0201 ...
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bioRxiv - Developmental Biology 2019Quote: ... Crude cell lysate was passed through a Sepharose column containing 5mL amylose resin (New England Biolabs) and the MBP-tagged GFP-NT-Lysenin fusion protein was eluted with fresh 10 mM maltose in 1X PBS (pH 7.4) ...
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bioRxiv - Bioengineering 2019Quote: ... DNA fragments containing the AOX1 promoter and gene were amplified by PCR using Vent polymerase (NEB) with primer combinations AOX1P-F and AOX1P-R for the promoter and AOX1G-F and AOX1G-R for the gene (Table S3).
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bioRxiv - Developmental Biology 2020Quote: ... followed by permeabilization in PBS containing 0.5% Triton X-100 and ribonucleosidevanadyl complex (New England Biolabs) on ice for 5 min ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.8 M guanidine HCl) containing 8 units/ml and Proteinase K (P8107S, New England Biolabs Inc.) was added freshly to the digestion buffer ...
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bioRxiv - Biochemistry 2021Quote: ... rinsed with PBS and incubated in PBS containing 3% molecular biology grade BSA (New England Biolabs) and 0.05% Tween-20 for 1 h at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... Phages containing the library were rescued using M13KO7 Helper Phage (New England Biolabs, Ipswich, MA, USA). Solution-phase bio-panning was conducted in order to minimize the disruption of a target protein ...
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bioRxiv - Biochemistry 2021Quote: ... media was replaced with media containing 3 µM SNAP-Cell block (New England BioLabs cat. # S9106S) and cells maintained at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... the reaction product was combined with 20 μl digestion mix containing 5 μl ExoI (NEB, M0293S), 5 μl HinFI (NEB ...
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bioRxiv - Biophysics 2022Quote: ... containing TwinStrep-SUMO at the N-terminus of S15 using the NEBuilder HiFi assembly protocol (NEB). TnsC clones were transformed into Escherichia coli BL21(DE3 ...
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bioRxiv - Genomics 2022Quote: ... the 1μl DNA was mixed with 22μl reaction buffer containing 1x phi29 reaction buffer (NEB, M0269S), 20μM random primers (Thermo ...
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bioRxiv - Synthetic Biology 2024Quote: The plasmid library containing static barcodes was generated by transforming high-efficiency competent cells (NEB C3019), then plating them onto a large surface area of LB-agar (∼30 10-cm petri dishes ...
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bioRxiv - Neuroscience 2024Quote: 10µg of a plasmid containing 807bp of Pcdh10 exon 1 sequence was linearized with SacII (NEB) in 1x CutSmart buffer (NEB) ...
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The RNA-binding protein RbpB is a central regulator of polysaccharide utilization in gut BacteroidesbioRxiv - Microbiology 2023Quote: ... 100 µL of CIP-mix containing 10 U of calf intestinal alkaline phosphatase (New England Biolabs) in CIP-buffer were added and beads incubated for 30 min at 37°C ...
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bioRxiv - Genomics 2023Quote: ... the DNA was treated in a 30 µL reaction containing 12 U T4 polynucleotide kinase (NEB), 1 mM ATP (NEB) ...
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bioRxiv - Genomics 2023Quote: ... The region containing the linker-mAID sequence was then amplified by PCR using Phusion polymerase (NEB) from the modified pMK287-mAID-Hygro plasmid and subcloned into the pL452 vector which harbors the Neomycin resistance cassette under the control of a PGK promoter ...
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bioRxiv - Molecular Biology 2023Quote: ... CX plasmid (Bukhari et al., 2016) and 5′UTR containing amplicon was digested with NheI (NEB) followed by ligation with T4DNA ligase (NEB) ...
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bioRxiv - Biochemistry 2023Quote: Plasmids containing the α and β genes were freshly transformed in E.Coli BL21-DE3 cells (NEB). Transformed cells were plated on LB agar plates containing 100ug/ml Carbenicillin and grown overnight at 370C overnight ...
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bioRxiv - Microbiology 2023Quote: ... the closed 215-bp virS-containing DNA fragment was open by cleavage with NdeI (NEB, Germany). To this end ...
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bioRxiv - Plant Biology 2023Quote: ... the pMIR319C containing pMW#2 reporter constructs were linearized with XhoI (R0146, New England Biolabs, USA) and integrated into the mutant HIS3 locus of the YM4271 host strain (Gift from Ram Yadav ...
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bioRxiv - Biochemistry 2023Quote: ... The HBB 5’ UTR containing mRNA was then capped using Vaccinia Capping enzyme (New England Biolabs) and 2′O-methylated using Vaccinia 2′O Methyltransferase (New England Biolabs) ...
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bioRxiv - Bioengineering 2023Quote: ... containing 10 μL 2X Luna® Universal Probe qPCR Master Mix (M3004, New England Biolabs, USA) (final concentration 1X) ...
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bioRxiv - Molecular Biology 2023Quote: ... the plasmid containing the origin DNA sequence was digested with HindIII and EcoRI (New England Biolabs) according to manufacturer’s protocol (Supplemental Figure S2A ...
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bioRxiv - Molecular Biology 2023Quote: ... KUNV-wt and their reporter-containing variants were linearized using AgeI-HF enzyme (NEB, United States) prior in vitro transcription ...
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bioRxiv - Molecular Biology 2023Quote: Endpoint PCR was performed in a 20 µL reaction volume containing 1× OneTaq Master Mix (NEB), 0.2 µM of each primer and 1 µL of 100-fold diluted cDNA ...
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bioRxiv - Cancer Biology 2023Quote: ... we prepared a digestion mix containing 100 nL of NlaIII restriction enzyme (NEB, cat. no. R0125L) and 50 nL of CutSmart buffer (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... Transposed DNA was amplified in a solution containing 1X NEBNextHigh Fidelity PCR Master Mix (NEB #M0541L), 0.5 μM of Ad1.1 universal primer ...
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bioRxiv - Biochemistry 2024Quote: ... was incubated in a final reaction volume of 200µl containing 1x T7 polymerase reaction buffer (NEB), 25U T7 polymerase (NEB) ...
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bioRxiv - Neuroscience 2024Quote: ... Gels were then placed in digestion solution containing proteinase K (8U/mL, New England BioLabs, P8107S) and digested for 4 hours at room temperature.
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bioRxiv - Plant Biology 2021Quote: ... or anti-MBP antibody (1:10.000, used with 1:10.000 secondary horseradish peroxidase-conjugated anti-mouse antibody; NEB).
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bioRxiv - Molecular Biology 2021Quote: ... Primary antibodies (Anti-3-hydroxybutyryllysine [PTM Biolabs 1201] ...
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bioRxiv - Molecular Biology 2021Quote: Myc-Tag (9B11) antibody (NEB 2276 S) and mouse IgG (Santa Cruz sc-2025 ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit polyclonal anti-lactyllysine antibody (PTM Biolabs), or mouse monoclonal anti-β-actin antibody (A5316 ...
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bioRxiv - Biochemistry 2020Quote: ... Anti-butyryllysine antibody (PTM BioLabs, PTM#301) at 1:2000 dilution was incubated with the membrane overnight at 4 °C ...