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Citations for New England Biolabs :
251 - 300 of 1885 citations for Recombinant Human C mer Proto Oncogene Tyrosine Kinase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... at 37 °C for 1 hour or first with 5 units T4 Polynucleotide Kinase with 25 mM ATP in 1 X T4 Kinase buffer (T4PNK, NEB, M0236S) at 37 °C for 30 min and then with XRN-1 ...
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bioRxiv - Cell Biology 2022Quote: Recombinant C-terminally FLAG-Tagged TEX264 was produced by PURExpress in vitro protein synthesis kit (E6800; New England Biolabs). Phosphorylation reaction containing TEX264-FLAG and CK2 (mixture of CK2A1 and CK2B ...
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bioRxiv - Neuroscience 2022Quote: All anti-tau monoclonal antibodies were generated by the hybridoma approach against recombinant tau aggregates (human full-length tau) encapsulated in the ACM Polymersomes (ACM Biolabs, Singapore) and purified by size exclusion chromatography (SEC ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA fragments were treated with T4 polynucleotide kinase (NEB), ligated to the linker (NEB Universal miRNA Cloning Linker ...
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bioRxiv - Microbiology 2019Quote: ... amplified fragment was phosphorylated using T4 polynucleotide kinase (NEB) and then ligated using T4 DNA ligase (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... and dephosphorylated using T4 Polynucleotide Kinase (M0201, NEB, USA) prior to isopropanol precipitation and resuspension in 10 mM Tris [pH8]) ...
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA was treated with T4 Polynucleotide Kinase (NEB) and rRNA depleted using an in-house protocol and cDNA library preparation was preceded by ultrasonic fragmentation ...
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bioRxiv - Molecular Biology 2021Quote: ... The oligos were radiolabeled by T4 polynucleotide kinase (NEB) with adenosine [γ32P]-triphosphate (6000 Ci/mmol ...
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bioRxiv - Genomics 2020Quote: ... 5′ end phosphorylation using T4 polynucleotide kinase (NEB, M0201L), (4 ...
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bioRxiv - Genomics 2020Quote: ... RNA overhangs are repaired with T4 Polynucleoide Kinase (NEB) with no ATP at 37°C for 20 min ...
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bioRxiv - Synthetic Biology 2020Quote: ... gDNAs were either phosphorylated using T4 Polynucleotide Kinase (NEB) in lab or phosphorylated guides were purchased from IDT ...
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bioRxiv - Bioengineering 2019Quote: ... 0.1 U/µL T4 Polynucleotide Kinase (T4 PNK, NEB), 1x CutSmart® buffer (NEB).
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bioRxiv - Plant Biology 2021Quote: ... oligos were end-labelled using T4 polynucleotide kinase (NEB) with γ-32P ...
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bioRxiv - Genetics 2020Quote: ... The oligonucleotides were phosphorylated with T4 polynucleotide kinase (NEB), annealed and inserted in the linearized vector with T4 DNA ligase (NEB) ...
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bioRxiv - Microbiology 2020Quote: ... and 4 μL T4 polynucleotide kinase (New England BioLabs) in a 100 μL reaction for 2 hours and purified using P-30 spin columns (BioRad) ...
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bioRxiv - Developmental Biology 2022Quote: ... and 5’ end-labeled using T4 polynucleotide kinase (NEB) and [γ-32P] ATP ...
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bioRxiv - Biochemistry 2022Quote: ... followed by KLD (Kinase, Ligase, and DpnI) digestion (NEB). Detailed primers used for TatA variant construction can be found in Supplemental Table 2 ...
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bioRxiv - Cell Biology 2022Quote: ... T4 Polynucleotide Kinase (1:10, #MO201S, New England Biolabs), T4 Ligase reaction buffer (#B0202S ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and phosphorylated using T4 polynucleotide Kinase (New England Biolabs). The phosphorylated DNA was cleaned and concentrated again as above and then ligated using T4 DNA Ligase (New England Biolabs) ...
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bioRxiv - Biophysics 2019Quote: ... and T4 polynucleotide kinase were also purchased from NEB. All other chemicals were purchased from Sigma-Aldrich (St ...
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bioRxiv - Molecular Biology 2019Quote: ... [γ32P]ATP and T4 polynucleotide kinase (New England Biolabs).
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bioRxiv - Genomics 2019Quote: ... The reverse primers are phosphorylated using Polynucleotide kinase (NEB).
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bioRxiv - Microbiology 2021Quote: ... 1 µl 10× T4 polynucleotide kinase (PNK) buffer (NEB), 6 µl of water ...
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bioRxiv - Cancer Biology 2021Quote: ... Insert oligonucleotides were phosphorylated using T4 polynucleotide kinase (NEB) at 37 °C for 1 hr and were annealed by heating to 95°C for 5 min and slowly cooled to room temperature ...
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bioRxiv - Pathology 2021Quote: ... then treated with Kinase-Ligase-DpnI (New England Biolabs) prior to heat shock transformation into chemically competent DH5α Escherichia coli cells (Table 1 ...
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bioRxiv - Molecular Biology 2020Quote: ... T4 polynucleotide kinase (New England Biolabs, with 1mM ATP) and Terminal exonuclease (buffer A ...
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bioRxiv - Genetics 2019Quote: ... Annealed oligos were phosphorylated by T4 polynucleotide kinase (NEB) for two hours at 37°C followed by 65°C for 20 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... T4 Polynucleotide kinase PNK 200 U/ml (NEB M0201L); DNA Polymerase I Large (Klenow ...
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bioRxiv - Cell Biology 2022Quote: ... Oligos were phosphorylated with T4 Polynucleotide Kinase (NEB M0201) at 37oC for 30 minutes ...
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bioRxiv - Plant Biology 2022Quote: ... Following phosphorylation with T4 polynucleotide kinase (New England BioLabs), the PCR products were ligated into expression vector pQE-1 (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... 5’ phosphorylated by T4 polynucleotide kinase (New England Biolabs), ligated to a blunt-end linear vector (typically also PCR products) ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were circularized with T4 kinase (NEB, M0201L) and T4 ligase (NEB ...
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bioRxiv - Cancer Biology 2023Quote: ... DpnI restriction enzyme and polynucleotide kinase were used (NEB). Some constructs were made using the Gateway cloning system (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... and 7 μl Polynucleotide Kinase (10 U/µl; NEB) in a total of 100 μl and incubation for 20 minutes at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: ... followed by KLD (Kinase, Ligase, and DpnI) digestion (NEB) by using primers deltaTatA_F (5’-GTGTTTGATATCGGTTTTAGC-3’ ...
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bioRxiv - Biophysics 2023Quote: ... and 10 U T4 polynucleotide kinase (New England Biolabs) in a final volume of 10 µL ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μL T4 polynucleotide kinase (PNK; New England Biolabs), 1 μL DpnI (New England Biolabs) ...
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bioRxiv - Microbiology 2023Quote: ... These primers were phosphorylated using T4 polynucleotide kinase (NEB) prior to PCR ...
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bioRxiv - Neuroscience 2023Quote: ... and were then phosphorylated using T4 polynucleotide kinase (NEB) according to the manufacturer’s manual ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by 5’ phosphorylation with T4 Polynucleotide Kinase (NEB) and self-ligation with T4 DNA Ligase (NEB).
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bioRxiv - Biophysics 2023Quote: ... The sample was phosphorylated using protein kinase A (NEB) and then further purified on size exclusion chromatography.
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bioRxiv - Cancer Biology 2023Quote: ... RNA was 3’ dephosphorylated using T4 Polynucleotide Kinase (NEB) and preadenylated linkers were ligated to the RNA fragments using T4 RNA Ligase 2 K277Q (NEB) ...
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bioRxiv - Developmental Biology 2023Quote: ... and end-labeled using T4 polynucleotide kinase (M0201, NEB) and [γ-32P] ATP (NEG002A250UC ...
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bioRxiv - Molecular Biology 2023Quote: ... and T4 poly nucleotide kinase (New England Biolabs, M0201L) and then ligated with 3ʹ RNA linker (with sample barcodes ...
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bioRxiv - Biochemistry 2024Quote: ... and 10 U T4 polynucleotide kinase (New England Biolabs) in a final volume of 10 µL ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant Cas9 protein (NEB) and sgRNA prepared by in vitro transcription were added to the mixture and incubated at 37℃ for ∼ 4h to remove rRNA ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... were coated to dry overnight at 37°C with 400 ng/well of recombinant His-tagged GT198 proteins together with 5 μg/well of purified BSA (NEB) in a volume of 50 μl ...
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bioRxiv - Cancer Biology 2019Quote: ... were coated to dry overnight at 37°C with 400 ng/well of recombinant His-tagged GT198 proteins together with 5 µg/well of purified BSA (NEB) in a volume of 50 µl ...
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bioRxiv - Cell Biology 2020Quote: ... Protein expression was induced with 0.3 mM IPTG for 2 hours at 37°C and recombinant MBP-rabaptin5 was purified with an amylose resin (New England Biolabs) according to manufacturs instructions and dialysed against lysis buffer (20 mM Tris-HCl ph7.4 ...
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bioRxiv - Microbiology 2020Quote: ... THUNDERBIRD Probe qPCR Master Mix (Toyobo) for MERS-CoV and Luna Universal Probe One-Step RT-qPCR Kit (NEB) for SARS-CoV-2.