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Citations for New England Biolabs :
251 - 300 of 4672 citations for High mobility group protein B1 HMGB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... pET17b-Kif5b(1-560)-GFP-His was transformed into BL-21[DE3]RIPL cells (New England Biolabs) until an optical density at 600 nm of 0.6-0.8 and expression was induced with 0.5 mM isopropyl-β-d-thiogalactoside (IPTG ...
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bioRxiv - Genomics 2020Quote: ... We put the mutagenized genes in a pIVEX vector via Gibson assembly (NEB Hi-Fi DNA assembly) using 125ng of gene DNA ...
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bioRxiv - Biochemistry 2021Quote: ... and C-terminal additions) were assembled with the pcDNA5 backbone using Hi-Fi DNA assembly (NEB; E2621S).
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bioRxiv - Microbiology 2019Quote: ... the product was cloned into the pET1-5b N-terminal 6×His expression plasmid (New England Biolabs).
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bioRxiv - Molecular Biology 2021Quote: ... The His-GST tags were cleaved by incubating the pooled fractions with TEV protease (New England Biolabs) at 4°C for 16h ...
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bioRxiv - Genetics 2019Quote: ... 2012) by removing cbr-Pmyo-2∷gfp∷his-72 UTR by cutting using KpnI and ApaI (NEB). The digested pZZ0031 backbone carrying cbr-unc-119(+ ...
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bioRxiv - Bioengineering 2022Quote: ... The t-NLuc-His tag was double-digested with NdeI and XhoI restriction enzymes (New England BioLabs), and the RNA sensor region were digested with SgrAI and SacII restriction enzymes ...
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bioRxiv - Microbiology 2023Quote: ... All 3 fragments were amplified from pTRL2-His-GrgA 36 using Q5 DNA polymerase (New England Biolabs). Fragment 1 was generated using primers pgp3-pgp4-F and His-RBS-R (Table S1) ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids were constructed in the pUC19 backbone using NEBuilder Hi-Fi DNA Assembly Mastermix (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... All 3 fragments were amplified from pTRL2-His-GrgA (28) using Q5 DNA polymerase (New England Biolabs). Fragment 1 was generated using primers pgp3-pgp4-F and His-RBS-R (sTable 5) ...
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bioRxiv - Genomics 2020Quote: ... Following the manufacturer’s instruction 3μg RNA per group was used for sequencing libraries were generated using a NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA). The index-coded samples were clustered on a cBot Cluster Generation System using the TruSeq PE Cluster Kit v3-cBot-HS (Illumina ...
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bioRxiv - Cell Biology 2019Quote: 40pmol of single stranded oligonucleotide was labelled at the free 5’-OH group with 20 units of T4 polynucleotide kinase (NEB # M0201) in a 50 μl reaction volume containing 1X polynucleotide kinase buffer and 30 μCi γ32P ATP at 37°C for 30min ...
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bioRxiv - Molecular Biology 2020Quote: ... was linked to the free hydroxyl group at the 3’-end of transcripts (1 □g of total RNA) by T4 RNA ligase 1 (NEB M0204) in the presence of 15% (w/v ...
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bioRxiv - Molecular Biology 2023Quote: ... oligonucleotides corresponding to each CTCF element were cloned into the pROSA-TV2 vector (created by Prof. Benjamin Davies group) between the pair of BsaI sites using the Golden Gate Assembly Kit (NEB, E1601) and this generates the HDR donor template containing the inserted CTCF element ...
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA was digested using SphI High Fidelity and MluCI High Fidelity enzymes with the appropriate CutSmart Buffer (New England Biolabs) and cleaned with AMPURE XP beads ...
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bioRxiv - Neuroscience 2021Quote: ... were used for amplification with Platinum Taq DNA Polymerase High Fidelity or Q5 High Fidelity DNA Polymerase (New England BioLabs), followed by cloning as per the TOPO TA Cloning Kit (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... the N gene was amplified from the BAC containing viral cDNA with a high fidelity polymerase (Taq Q5 Hot Start High-Fidelity 2x Master Mix, Ref. M0494S, NEB) and primers PV012-F (5’-ACT-GTA-ATA-CGA-CTC-ACT-ATA-GGG-ATG-TCT-GAT-AAT-GGA-CCC-CAA-AAT-C-3’ ...
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bioRxiv - Systems Biology 2021Quote: ... PCR reactions with plasmid and genomic DNA templates were performed using the Phusion High-Fidelity 2× Master Mix or Q5 High-Fidelity 2× Master Mix (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... genomic DNA (5-10 μg) derived from transfected ESC was digested by high concentrated HindIII-HF or high concentrated BamHI-HF (NEB). In all Southern blot analyses ...
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bioRxiv - Genomics 2024Quote: Between 2 and 4 million cells were harvested by centrifugation and high molecular weight (HMW) genomic DNA was extracted using the Monarch High Molecular Weight DNA Extraction kit (NEB) and agitation speeds of 1,500 rpm.
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bioRxiv - Microbiology 2020Quote: ... or with Q5 High fidelity DNA polymerase (NEB) when the product required high fidelity polymerase.
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bioRxiv - Molecular Biology 2021Quote: Q5® High-Fidelity DNA Polymerase (NEB, M0491S) was used to perform PCR reactions following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Q5 High-Fidelity DNA Polymerase (New England Biolabs) was used for all cloning PCR reactions ...
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bioRxiv - Developmental Biology 2020Quote: ... and Q5 high-fidelity polymerase (New England Biolabs) and comprised the following cycling parameters ...
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bioRxiv - Microbiology 2021Quote: Phusion High-Fidelity DNA polymerase (New England BioLabs) was used for all plasmid constructions and all plasmid sequences were confirmed by Sanger sequencing ...
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bioRxiv - Microbiology 2021Quote: ... Phusion High-Fidelity DNA polymerase (New England BioLabs) was used for all plasmid constructions and all plasmid sequences were confirmed by Sanger sequencing ...
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bioRxiv - Cancer Biology 2020Quote: ... using Q5 High-Fidelity Master Mix (NEB, M0492) and the following primers ...
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bioRxiv - Cancer Biology 2019Quote: ... Phusion high fidelity master mix (New England BioLabs) was used with 10 μM of each primer and 1 ng gBlock or 50 ng genomic DNA and amplified for 25 cycles ...
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bioRxiv - Microbiology 2021Quote: ... we used Q5 High-Fidelity DNA polymerase (NEB). For diagnostic PCR ...
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bioRxiv - Neuroscience 2020Quote: ... PCR master mixes (NEBNext High fidelity, M0541S, NEB) containing unique barcoding primers per well were dispensed on top of the reverse crosslinking buffer and DNA was amplified with the following cycling conditions ...
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bioRxiv - Bioengineering 2021Quote: ... Q5 High-Fidelity DNA polymerase (New England BioLabs), and Velocity DNA polymerase (Bioline ...
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bioRxiv - Biochemistry 2020Quote: ... Phusion High-Fidelity DNA Polymerase (1 U, NEB), deoxynucleotide solution mix (dNTP ...
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bioRxiv - Cell Biology 2020Quote: Q5 high fidelity polymerase (M0492S, New England Biolabs) was used for the PCR reaction (95°C 3min ...
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bioRxiv - Neuroscience 2020Quote: ... and a high-fidelity Phusion DNA polymerase (NEB) and cloned into either pCMV vectors using Gibson Assembly or into pCR2.1 TOPO (Thermo Fisher ...
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bioRxiv - Plant Biology 2021Quote: ... using Q5® High-Fidelity DNA Polymerase (NEB). The vector pNOC-642 linearized using SacI (NEB ...
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bioRxiv - Microbiology 2021Quote: ... PCR with Phusion High Fidelity DNA Polymerase (NEB) or iProof DNA Polymerase (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... Q5 High-Fidelity DNA Polymerase (New England Biolabs) was used ...
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bioRxiv - Plant Biology 2020Quote: ... or Q5 High-Fidelity DNA Polymerase (NEB, M0419S) were used to generate the following constructs in this study ...
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bioRxiv - Microbiology 2021Quote: ... using Q5 High Fidelity Polymerase (New England Biolabs) using the primers listed in Suppl ...
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bioRxiv - Immunology 2022Quote: ... High-fidelity Phusion polymerase (New England Biolabs, USA) was used for all DNA amplification.
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bioRxiv - Molecular Biology 2022Quote: ... Q5 high-fidelity DNA polymerase (New England BioLabs) was used in the PCR reaction ...
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bioRxiv - Cell Biology 2022Quote: ... or Amylose Resin High Flow (New England Biolabs) respectively according to the manufacturers’ instructions.
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bioRxiv - Developmental Biology 2022Quote: ... using with Q5 High Fidelity master mix (NEB) and subcloned into pWPXLd (Addgene #12258 ...
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bioRxiv - Molecular Biology 2020Quote: ... High fidelity DNA polymerase (NEB, cat. no. M0492S) was used to generate all the constructs ...
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bioRxiv - Immunology 2020Quote: ... or Phusion® High Fidelity (New England Biolabs) DNA polymerases ...
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bioRxiv - Cancer Biology 2022Quote: ... Q5 High-Fidelity 2X Master Mix (NEB #M0492S) was used ...
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bioRxiv - Bioengineering 2022Quote: ... Q5 high-fidelity DNA polymerase (New England Biolabs) was used for site-directed mutagenesis42 ...
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bioRxiv - Synthetic Biology 2019Quote: ... and 1ul high concentration T4 DNA ligase (NEB). The reaction was incubated for 50 cycles at 37C for 3min ...
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bioRxiv - Microbiology 2019Quote: ... PCRs used the high-fidelity Q5 polymerase (NEB) per suggested protocols ...
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bioRxiv - Biochemistry 2020Quote: ... Phusion High-Fidelity DNA Polymerase (1 U, NEB), deoxynucleotide solution mix (dNTP ...