Labshake search
Citations for New England Biolabs :
251 - 261 of 261 citations for Genz 644282 CAS 529488 28 6 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2023Quote: ... were synthesized by Gen9 Bio (San Jose, CA) and amplified by PCR using Q5 High-Fidelity DNA Polymerase (New England BioLabs) and gene-specific primers from Integrated DNA Technologies (Supplementary Table 11) ...
-
bioRxiv - Immunology 2023Quote: ... Completed libraries were then sequenced to an average depth of approximately 20M reads per sample on a partial lane of the NovaSeq6000 S4 XP flow cell using 2×150 paired-end reads with 10-base dual indexes (CAS: E6440S, NEB). After demultiplexing these samples ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: The colorectal carcinoma MC38 murine model studies were conducted at BioDuro (at Explora BioLabs’ vivarium in San Diego, CA, USA). The MC38 cell line was obtained from Kerafast (Boston ...
-
bioRxiv - Genomics 2020Quote: ... The PCR product and pGAD-C1 vector were digested with ClaI (5’-ATCGAT-3’, New England BioLabs Inc., MA, CA#R0197S) and SalI (5’-GTCGAC-3’ ...
-
bioRxiv - Bioengineering 2021Quote: ... homology arms were amplified from genomic DNA and introduced to the MCS of the linearized SIBR-Cas plasmid using the NEBuilder® HiFi DNA Assembly Master Mix (NEB). The plasmid was linearized using Esp3I (NEB) ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... about 1000 bp upstream and downstream of the cas operon was amplified from MGH21 using Q5 DNA polymerase (NEB, Cat# M0492). Overlap extension PCR was used to fuse these two pieces of DNA to generate a ~ 2000 bp fragment which was then ligated to pKOV vector51 using BamHI and NotI sites ...
-
bioRxiv - Cancer Biology 2023Quote: DNA from in vitro and in vivo experiments was sent to Novogene (Sacramento, CA) and library preparation was performed using the NEBNext® DNA Library Prep Kit (New England Biolabs) following the manufacturer’s recommendations ...
-
bioRxiv - Pathology 2022Quote: ... Total RNA was subjected to two rounds of poly(A) selection using oligo-d(T)25 magnetic beads (New England Biolabs, CA, USA). Illumina compatible RNAseq library was prepared using NEB next ultra RNAseq library preparation kit ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... the complete genome sequences were amplified for CYVCV CA isolates using the Q5 high-fidelity enzyme (New England Biolabs Inc., MA, USA) and virus-specific PCR primers (5′ primer-GAAAAGCAAACATAACCAACACACACCC ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... we isolated DNA from tissue using the Qiagen DNeasy Blood and Tissue kit (Valencia, CA, USA) and created genomic libraries using the NEBNext Ultra II kit (New England BioLabs; Ipswich, MA, USA), according to manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2024Quote: ... the required PHL7 codon-optimized sequence was purchased from IDT (Integrated DNA Technologies, San Diego, CA, USA) and integrated into the expression vectors by NEBuilder® HiFi DNA Assembly (NEB - New England Biolabs). The backbone was prepared by PCR using the protocol described in (dx.doi.org/10.17504/protocols.io.bprimm4e) ...