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Citations for New England Biolabs :
2901 - 2950 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... NEBNext Ultra RNA Library Prep Kit for Illumina (New England BioLabs Inc.) was used for library preparation ...
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bioRxiv - Microbiology 2021Quote: ... DHFR-TS:HXGPRT backbone by NEBuilder HIFi DNA Assembly Cloning Kit (NEB, E5520S) after double enzymatic digestion of KpnI and NdeI ...
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bioRxiv - Cancer Biology 2021Quote: ... Final libraries were quantified using NEBNext Library Quant Kit for Illumina (NEB) and high sensitivity D1000 TapeStation (Agilent) ...
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bioRxiv - Bioengineering 2020Quote: RT-LAMP reactions were carried out with the WarmStart LAMP Kit (NEB) using the manufacturer’s recommended protocol ...
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bioRxiv - Genomics 2021Quote: ... and cloned into plasmid pT2 using Gibson Assembly Cloning Kit (NEB, E5510S). Obtained pT2_FF plasmids were used as templates for PCR-based barcoded library preparation (22).
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bioRxiv - Biophysics 2021Quote: ... reactions were prepared using the PURExpress Δ Ribosome Kit (New England Biolabs) in volumes of 5 µL and were allowed to proceed for 15 min at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... were generated using Q5® Site-Directed Mutagenesis Kits (New England BioLabs) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Using the NEB Next Ultra RNA Library Prep Kit for Illumina (NEB), which selects for poly(A ...
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A small molecule reveals role of insulin receptor-insulin like growth factor-1 receptor heterodimersbioRxiv - Cell Biology 2021Quote: ... RNA extraction was performed using the Monarch Total RNA Miniprep Kit (NEB), according to manufacturer’s protocol ...
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bioRxiv - Physiology 2020Quote: ... or the Q5 Site-Directed Mutagenesis kit (New England Biolabs, Ipswich, MA). The mutated constructs were then digested with restriction endonucleases to excise the mutated fragments ...
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bioRxiv - Cell Biology 2021Quote: ... libraries were prepared using the NEBNext Ultra RNA library Prep Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... was generated via site-directed mutagenesis (NEB, Q5 Site-Directed Mutagenesis Kit). using primers listed in the primer table (Table S1) ...
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bioRxiv - Plant Biology 2021Quote: ... Constructs were assembled using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB) and their integrity verified by Sanger sequencing ...
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bioRxiv - Microbiology 2021Quote: ... 1 µg RNA was reverse transcribed using LunaScript RT SuperMix Kit (NEB). qPCR was performed using synthesised cDNA ...
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bioRxiv - Genetics 2020Quote: ... This cloning step was performed using Gibson Assembly kit (New England Biolabs). This yielded the chimeric PaKmt6-GFP-HA allele ...
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bioRxiv - Microbiology 2021Quote: ... using Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) with an in-house developed protocol ...
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bioRxiv - Microbiology 2021Quote: ... Cloning was performed using the NEBuilder HiFi DNA Assembly kit (NEB, E2621).
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bioRxiv - Microbiology 2019Quote: ... (2) DSBs blunting with NEB’s Quick Blunting Kit (NEB, cat. number: E1201); (3 ...
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bioRxiv - Molecular Biology 2020Quote: ... a mix of 4 μl First strand Synthesis Reaction Buffer (NEB-kit), 0.5 μl Murine RNase Inhibitor (NEB-kit ...
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bioRxiv - Molecular Biology 2020Quote: ... A mix of 5 μl 3’Ligation Reaction Buffer 2x (NEB-kit) and 1.5 μl of 3’Ligation Enzyme Mix (NEB-kit ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA was synthesized using Protoscript II First Strand cDNA synthesis kit (NEB). The expression of target genes was determined by RT-qPCR in Applied Biosystems 7500 using the 2−ΔΔCT method normalized by the expression of 18s rRNA ...
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bioRxiv - Plant Biology 2021Quote: ... Library construction (NEBNext Ultra RNA Library Prep Kit, New England Biolabs Inc.) from 1 µg RNA ...
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bioRxiv - Molecular Biology 2020Quote: ... After 24h RNA was isolated using Monarch Total RNA miniprep Kit (NEB) and reverse transcribed to cDNA using a Precision Reverse Transcription kit (PrimerDesign ...
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bioRxiv - Microbiology 2020Quote: ... or Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) or LightCycler® Multiplex RNA Virus Master (Roche ...
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bioRxiv - Microbiology 2020Quote: ... The Monarch® DNA Gel Extraction Kit (New England BioLabs, Ipswitch, MA) was used to isolate PCR products during PCR ...
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bioRxiv - Cancer Biology 2020Quote: ... Sequencing libraries were prepared using the NEBNext Ultra kit (New England Biolabs), according to the manufacturer’s instructions using 300ng fragmented DNA input for each sample ...
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bioRxiv - Cell Biology 2021Quote: ... Cdc11a T62A was generated using the Q5-site directed mutagenesis kit (NEB).
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bioRxiv - Biophysics 2020Quote: We used in vitro mutagenesis (Q-5 Site-directed Mutagenesis Kit, NEB) to introduce the mutations creating d isoforms using the following primers ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA was generated using Postscript II 1st strand cDNA synthesis kit (NEB). Coding sequences were PCR amplified from cDNA or vectors with Q5 high-fidelity Taq Polymerase (NEB ...
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bioRxiv - Plant Biology 2020Quote: ... or NEBNext Ultra II Directional RNA Library Prep Kit (New England BioLabs) was used for library construction with 500 ng of treated RNA ...
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bioRxiv - Neuroscience 2022Quote: ... or the NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350, NEB) (replicates 4-5 and no salt treatment ...
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bioRxiv - Microbiology 2022Quote: ... Affinity tags were introduced by NEBuilder HiFi DNA Assembly Cloning Kit (NEB) into the expression vectors encoding Rae1 and Nup98 using primers in Table 1 ...
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bioRxiv - Cell Biology 2022Quote: ... Cleanup IVT reaction using Monarch RNA cleanup kit (New England BioLabs – T2040L), eluting in 12 μL NFW ...
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bioRxiv - Immunology 2022Quote: RNA from MΦ was isolated using Monarch total RNA MiniPrep kit (BioLabs) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was extracted with the Monarch Total RNA Miniprep Kit (NEB #T2010), following the manufacturer’s indications ...
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bioRxiv - Cancer Biology 2022Quote: ... cDNA was synthesized using LunaScript® RT SuperMix Kit (New England Biolabs). Gene expression to validate RNA-seq analysis was quantified by qRT-PCR using Luna® Universal qPCR Master Mix (New England Biolabs ...
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bioRxiv - Developmental Biology 2022Quote: ... and then cleaned up using the Monarch PCR Cleanup kit (T1030L, NEB). Digested PCR products were then ligated to the digested pcs2+MCS-P2A-sfGFP plasmid using T4 DNA ligase (M0202S ...
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bioRxiv - Genomics 2022Quote: ... PCR products were purified by gel extraction (Monarch gel extraction kit, NEB), and assembled into the backbones using NEB HiFi DNA Assembly master mix kit ...
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bioRxiv - Genetics 2022Quote: ... The NEBuilder High-Fidelity DNA Assembly Cloning Kit (New England Biolabs, E5520) was used to assemble DNA fragments into the targeting construct based on manufacturer’s instructions with the primers detailed in Supplemental Table 1 ...
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bioRxiv - Genomics 2022Quote: ... DNA fragmented by sonication was repaired using Quick Blunting Kit (NEB: E1201S). For TA ligation ...
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bioRxiv - Biochemistry 2022Quote: ... Following which the PCR products were purified by PCR cleanup kit (NEB) and a second PCR was done with primers containing the READ1 ...
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bioRxiv - Genetics 2022Quote: ... using the Q5 site directed mutagenesis kit (New England Biolabs, catalogue # E0554S). The resulting modified LexA (mLexA ...
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bioRxiv - Microbiology 2020Quote: ... Using the standard protocol of the Q5 Site-directed Mutagenesis kit (NEB), the region of pTA1228 spanning restriction sites NdeI and BamHI was replaced with KasI yielding the new plasmid pTA1300 ...
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bioRxiv - Biochemistry 2021Quote: ... which were assembled using the Gibson Assembly Cloning Kit (New England Biolabs). Recombinant HWT and HI473D were expressed in E ...
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bioRxiv - Developmental Biology 2021Quote: ... Capped and tailed mRNAs were purified by Monarch RNA Cleanup Kit (NEB) according to the manufactory guide and eluted in DEPC-treated water ...
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bioRxiv - Microbiology 2021Quote: Cloning was performed using NEBuilder HiFi DNA Assembly kit (NEB, cat # E2621). Both shuttle vectors were propagated in E ...
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bioRxiv - Biochemistry 2021Quote: ... The two fragments were ligated using a DNA HiFi assembly kit (NEB). All plasmid constructs were confirmed by sequencing (Genewiz).
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bioRxiv - Biochemistry 2021Quote: ... and immunoprecipitation was performed using the EpiMark N6-methyladenosine Enrichment Kit (NEB) as per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... PCR products were purified by gel extraction (Monarch gel extraction kit, NEB), and assembled in a Golden Gate assembly containing 6.25 ng pU6-atgRNA-GG-acceptor (Addgene #132777) ...
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bioRxiv - Microbiology 2021Quote: ... and were ligated into pAY5211 employing the Quick LigationTM Kit (NEB, USA), resulting in the targeting plasmid (pTargeting) ...