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Citations for New England Biolabs :
2801 - 2850 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... RNA was purified using the Monarch RNA clean-up kit (NEB) and RNA length and quality was verified via 10% acrylamide gel.
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bioRxiv - Immunology 2023Quote: sgRNAs were produced using the transcription T7 high yield kit (NEB) and purified with the EZNA micro-elute RNA clean up kit (Omega Bio-tek) ...
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bioRxiv - Molecular Biology 2023Quote: Plasmids were constructed using a NEBuilder HiFi DNA Assembly kit (NEB) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and NEBnext Ultra II Directional RNA library prep kits (NEB #E7760L). Paired end 200 bp sequencing with additional reads for dual 8/8 indices plus the 11nt UMI after the i7 index was performed on an Illumina NovaSeq 6000 at the University of Chicago Genomics Facility.
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bioRxiv - Microbiology 2024Quote: ... Fragments were purified using a gel agarose purification kit (NEB Monarch). Taq polymerase PCR with primers P7 and P8 ...
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bioRxiv - Microbiology 2024Quote: ... using Q5® Site-Directed Mutagenesis Kit (New England Biolabs; #E0554S). Guide RNA and repair template sequences are listed in Supplementary Data 3c.
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bioRxiv - Cancer Biology 2024Quote: ... using the NEB Ultra II Library Prep Kit (New England Biolabs) per manufacturer’s instructions and the libraries were sequenced at 50 bp read length and were sequenced on an NovaSeq SP with a target of ∼40M PE reads per sample.
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bioRxiv - Cancer Biology 2024Quote: ... using the NEB Ultra II Library Prep Kit (New England Biolabs) per manufacturer’s instructions and sequenced using Hiseq 4000 PE 150bp ...
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bioRxiv - Genomics 2024Quote: ... Libraries were constructed using the NEBNext Enzymatic Methyl-seq Kit (NEB), following the manufacturer’s guidance ...
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bioRxiv - Microbiology 2024Quote: ... using the NEBNext Library Quant Kit for Illumina (New England BioLabs) according to the instructions of the manufacturer ...
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bioRxiv - Neuroscience 2024Quote: ... and then quantified by qPCR (NEBNext Library Quant Kit, NEB, E7630). 20 pM of DNA were loaded onto an Illumina MiSeq (v3 ...
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bioRxiv - Immunology 2024Quote: ... and gRNAs were purified with the Monarch RNA Cleanup Kit (NEB), aliquoted ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmid extraction and DNA purification were performed using Monarch kits (NEB). Sequences were verified with Sanger sequencing by Azenta Life Sciences or Primordium Labs for whole plasmid sequencing ...
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bioRxiv - Synthetic Biology 2024Quote: ... Genomic DNA was extracted using Monarch Genomic DNA Purification Kit (NEB) according to manufacturer’s instructions and eluted in 20 µL water.
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bioRxiv - Microbiology 2024Quote: ... using the NEBNext Ultra II DNA Library prep Kit (E7370; NEB). Libraries were sequenced using an Illumina NextSeq 2000 sequencer 75 bp Single End.
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted using the Monarch Genomic DNA Purification Kit (NEB), and quality checked using a genomic Tapestation 4200 (Agilent Technologies) ...
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bioRxiv - Microbiology 2024Quote: ... Gibson Assembly® Cloning Kit was employed (New England BioLabs Inc) to make the deletion construct pEX-18Tc-femI ...
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bioRxiv - Microbiology 2024Quote: ... and cloned into pLGB13 using the NEBuilder HiFi cloning kit (NEB). The pLGB13 plasmid was linearized by double digest with BamHI and SalI restriction enzymes ...
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bioRxiv - Microbiology 2024Quote: ... using the 2X Gibson Assembly Kit (Cat # E2611S, New England Biolabs). Assembled constructs were confirmed by whole plasmid sequencing (Plasmidsaurus ...
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bioRxiv - Microbiology 2024Quote: ... and Biolux Cypridina luciferase assay kit (New England Biolabs, USA; #E3309), respectively ...
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bioRxiv - Cell Biology 2024Quote: ... or the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, E0554), the DpnI (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... dsRNA was purified using Monarch RNA Cleanup Kit (50 μg; NEB) and checked on 1% agarose gel and concentration measured with a NanoDrop One spectrophotometer (ThermoFisher) ...
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bioRxiv - Molecular Biology 2024Quote: ... LongAmp® Hot Start Taq DNA Polymerase kit (New England Biolabs) was used according to the manufacturer’s protocol to amplify the SEGS-1 region from DNA samples collected from 4 plants of each genotype ...
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bioRxiv - Molecular Biology 2024Quote: Genomic DNA was used with the EpiMark Bisulfite Conversion Kit (NEB) using the manufacturer instructions.
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bioRxiv - Genomics 2024Quote: ... the Monarch® HMW DNA Extraction Kit for Tissue (NEB, USA) was utilized in accordance with the manufacturer’s specifications ...
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bioRxiv - Genomics 2024Quote: Total RNA was extracted with Monarch Total RNA Miniprep Kit (NEB) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... with the NEBNext Ultra II Directional RNA Library Prep kit (NEB). One of the three H ...
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bioRxiv - Cell Biology 2024Quote: ... and purified using an NEB clean and concentrate kit (NEB #T2030).
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bioRxiv - Synthetic Biology 2024Quote: ... and purified with the Monarch® PCR & DNA Cleanup Kit (NEB). An aliquot (1 µg ...
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bioRxiv - Microbiology 2024Quote: ... or the Monarch® DNA Gel Extraction Kit (New England Biolabs). In a few cases ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA synthesis was performed using LunaScript® RT SuperMix Kit (NEB) following provider recommendation ...
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bioRxiv - Biochemistry 2020Quote: ... A pre-cleaning step was applied by incubating each sample with 10 μl of Protein A magnetic beads (New England Biolabs GmbH, Frankfurt, Germany) for 1 h at 4 °C in tube roller (15 rpm ...
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bioRxiv - Cell Biology 2022Quote: ... The DNA encoding the EGFP-fusion proteins and the P2A peptide was inserted by Gibson assembly using NEBuilder HiFi DNA assembly master mix (New England BioLabs, Ipswich, MA, USA). The HeLa Flp-In T-REx K44A Dynamin2-EGFP-P2A-Caveolin1-mCherry construct was obtained by in vitro mutagenesis of the pcDNA/FRT/TO/Dyn2-EGFP-P2A-Caveolin1-mCherry construct exchanging lysine at position 44 to an alanin using these primers ...
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bioRxiv - Developmental Biology 2020Quote: ... A total of 863 Bicyclus embryos were injected with 300 ng/µl of guide and 300 ng/µl of Cas9 protein (NEB; Cat. no.: M0641) mixed together in equal parts (total volume = 10 µl ...
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bioRxiv - Developmental Biology 2019Quote: ... The bound protein (~42kDa, 360 amino acids) was released from the MBP tag by cleavage with Factor Xa (New England Biolabs, Massachusetts, United States) for 24 hours at 4°C in 20mMTris pH 7.2 ...
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bioRxiv - Cancer Biology 2020Quote: ... The synthetic genes encoding WT human TRIM28 with an N-terminal maltose binding protein (MBP) affinity tag (UniProt: Q13263) were subcloned to the pMAL-p2X vector (New England Biolabs, Beverly, MA, USA).
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bioRxiv - Bioengineering 2023Quote: ErCas12a (1 μg) or LbCas12aU (1 μg) protein and 1 μg crRNA were assembled in 1X Nuclease Reaction Buffer (New England BioLabs, Ipswich, MA, USA) at room temperature for 10 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... corresponding to a portion of PfRNF1 (RP2; Fig. 1A) was expressed as a maltose-binding fusion protein using the pMAL™c5X-vector (New England Biolabs, Ipswich, USA). To this end ...
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bioRxiv - Microbiology 2021Quote: The rem allele was amplified from a cloning construct (primer sequences in Table S4) and ligated into pTYB12 (NEB IMPACT system of protein purification), resulting in an N-terminal intein fusion to Rem ...
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bioRxiv - Microbiology 2021Quote: ... MNase activity was quenched by addition of EGTA and samples were subsequently pre-cleared by addition of 40 µl Protein A Magnetic Beads (New England Biolabs, Ipswich, MA, United States) and rotating at 4°C for 60 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Removal of both N-linked and O-linked glycans was performed by incubation with five different glycosidases: PNGaseF (New England Biolabs, 125 U/μg protein), O-glycosidase (New England Biolabs ...
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bioRxiv - Bioengineering 2022Quote: ... Processing the samples for proteomics was then continued using a 10 kDa filter aided sample preparation protocol previously published with minor modifications.[30] The proteins were digested using Lys-C enzyme (P8109S, New England Biolabs, MA, USA; 1:50) and then with trypsin (V5111 ...
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bioRxiv - Biophysics 2019Quote: ... The resulting product (typically 50 – 100 μL of 5 – 20 μM of protein) was mixed with an equimolar amount of SNAP-Surface 549 (New England Biolabs; 1 mM in DMSO) and incubated at room temperature for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... aliquots of the same protein extract were separated and treated with 200 U of Lambda Phage Phosphatase (#P0753S, New England Biolabs, Ipswich, Massachusetts, United States), as specified by the manufacturer ...
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bioRxiv - Genetics 2021Quote: ... 5 μg of MBP-Rif2 or MBP-Rif2-min (see recombinant protein preparation) was incubated for 1 h at 4 °C with amylose resin (New England Biolabs, 50 μl per reaction). The resin with the immobilized MBP-Rif2 variants was washed 5 times with 1 ml wash buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Plant Biology 2024Quote: ... when OD600 reached 0.5 for 4 h at 37 °C and then they were purified using amylose resin column (New England Biolabs, for MBP related proteins purification) or Ni-NTA agarose column (Qiagen ...
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bioRxiv - Developmental Biology 2023Quote: Injections into fertilized eggs for knockout experiments were done using 50ng/µl of each sgRNA independently and 80nM of NEB EnGen Spy Cas9-NLS protein (New England Biolabs France Genopole, Evry, France), pre-incubated at room-temperature for 15 minutes (Hill et al. ...
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bioRxiv - Plant Biology 2020Quote: ... ChIP DNA was purified with the Monarch PCR & DNA Cleanup Kit (NEB). Libraries were prepared using ThruPLEX DNA-seq Kit (Rubicon Genomics ...
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bioRxiv - Plant Biology 2020Quote: ... were mixed for DNA assembly using the Golden Gate assembly kit (NEB).
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were generated using NEBNext Ultra II DNA Library Prep Kit (NEB), modified for CUT&RUN as previously described(54) ...