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Citations for New England Biolabs :
2851 - 2900 of 9451 citations for Human Zyxin ZYX ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... The Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, MA, USA) was used to process the samples according to the protocol “NEBNext Ultra II Directional RNA Library Prep Kit for Illumina” ...
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bioRxiv - Biophysics 2021Quote: Point mutations in all constructs were made by using Q5 Site-Directed Mutagenesis Kit (NEB). Primers were designed with NEBase Changer ...
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bioRxiv - Developmental Biology 2020Quote: ... and T7 RNA polymerase from HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB). Cold-anesthetized female mosquitoes were microinjected with 2.0 µg gene-specific dsRNA using a Nanoject II microinjector (Drummond Scientific Company ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA synthesis was carried out by ProtoScript-II first strand cDNA synthesis kit (NEB, USA) with random hexamer primers ...
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bioRxiv - Microbiology 2021Quote: ... pLentiCRISPR-V2 backbone (Sanjana, Shalem and Zhang 2014) using Quick Ligation™ kit (M2200, NEB) and transduced into THP-1 WT cells using Lentiviral particles (Fisch et al ...
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bioRxiv - Microbiology 2020Quote: ... The resulting fragment was cloned using the NEBuilder HiFi DNA assembly kit (NEW ENGLAND BioLabs) into the pMiniMAD2 plasmid previously digested using the restriction sites BamHI and EcoRI ...
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bioRxiv - Biochemistry 2020Quote: ... The RNA was then purified using a Monarch RNA Clean-up Kit (New England BioLabs).
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bioRxiv - Biochemistry 2020Quote: DNA was purified using the Monarch® Genomic DNA Purification kit (New England BioLabs, T3010S). DNA immunoprecipitation and sequencing was performed as previously described ...
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bioRxiv - Bioengineering 2021Quote: Site directed mutagenesis was performed using Q5® Site-Directed Mutagenesis Kit from NEB (E0554S) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... We used NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, NEB) and followed the manufacturer’s manual with several exceptions:
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bioRxiv - Bioengineering 2021Quote: ... We used NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, NEB) and followed the manufacturer’s manual with several exceptions:
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bioRxiv - Bioengineering 2020Quote: RNA were transcribed using the HiScribe T7 high yield RNA synthesis kit (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Library construction was performed using the NEBNext UltraII DNA Library Prep Kit from NEB (E7645S). Indexed samples were run using the Illumina Next-seq 300-cycle kit ...
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bioRxiv - Biochemistry 2020Quote: ... the two DNA fragments were joined using Gibson Assembly® Cloning Kit (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Genes of interest were amplified with the One Taq One-Step RT-PCR kit (NEB) using 1 μg of RNA ...
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bioRxiv - Cell Biology 2020Quote: ... Library concentration and quality control were determined using NEBNext Library Quant Kit (New England Biolabs) and Agilent High Sensitivity D1000 ScreenTape System (Agilent ...
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bioRxiv - Molecular Biology 2021Quote: ... and cloned into a pcDNA3.1(+) vector using the Quick Ligation Kit (New England Biolabs M2200).
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bioRxiv - Biophysics 2020Quote: ... in-vitro transcription using the commercial HiScribe™ T7 High Yield RNA Synthesis Kit (NEB) gave rise to two complementary ssRNAs without any non-complementary nucleotides at their ends ...
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bioRxiv - Cancer Biology 2021Quote: ... The libraries were quantified using the NEBNext Library Quant Kit for Illumina (New England Biolabs) on a StepOne Plus Real-Time PCR (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2020Quote: ... Libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB E7645S) and NEBNext Multiplex Oligos for Illumina (NEB Set 1 E7335 and Set 2 E7500S) ...
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bioRxiv - Cancer Biology 2020Quote: ... The DNA fragments were ligated to Bar Code Adapters using Quick Ligation kit (NEB M2200L) and purified using Agentcourt AMPure Beads (Beckman Coulter ...
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bioRxiv - Cell Biology 2021Quote: ... Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Biochemistry 2021Quote: ... and 2 μL T7 RNA Polymerase mix (HiScribe T7 High Yield RNA Synthesis Kit, NEB) were incubated at 37°C for 3 h ...
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bioRxiv - Bioengineering 2021Quote: ... The synthesized mRNA was purified using Monarch RNA Cleanup Kit (New England Biolabs, Ipswich, MA), thereby removing the template DNA ...
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bioRxiv - Cell Biology 2021Quote: ... Site-directed mutagenesis was performed using the Q5® site directed mutagenesis kit protocol (NEB). GST-LL5β was cloned into pGEX plasmid vector ...
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bioRxiv - Cell Biology 2021Quote: ... Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: ... The DNAse-treated RNA samples were then placed through a Monarch RNA Cleanup Kit (NEB) to ensure no carryover of contaminants ...
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bioRxiv - Synthetic Biology 2021Quote: ... The resulting DNA templates were column purified using a Monarch PCR&DNA Cleanup Kit (NEB) and quantified by their absorption on a NanoPhotometer (Implen) ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA libraries were constructed using NEB next ultra-directional library kit (New England Biolabs Inc) and sequencing was performed using the Illumina HiSeq X platform ...
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bioRxiv - Microbiology 2020Quote: ... DNA ends were repaired using the NEBNext Ultra II End Repair Kit (New England Biolabs) and a DNA linker (26 ...
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bioRxiv - Microbiology 2021Quote: ... Point mutations were introduced by site-directed mutagenesis using Phusion High-Fidelity PCR kit (NEB) using primers listed in Table S1.
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bioRxiv - Neuroscience 2020Quote: ... RNA was reverse-transcribed to cDNA using M-MuLV Reverse Transcriptase kit (NEB, Ipswich, MA) and cDNA was analyzed with QPCR.
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bioRxiv - Genetics 2021Quote: ... PCR amplicons were purified using the Monarch PCR & DNA cleanup kit (New England BioLabs T1030) or the QIAQuick PCR purification kit (Qiagen 28104 ...
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bioRxiv - Neuroscience 2020Quote: ... Insert and vector fragments were ligated using HiFi DNA Assembly Kit (New England Biolabs, USA) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... To convert T05C1.4b cDNA to T05C1.4a we used the Q5 Site-Directed Mutagenesis Kit (NEB) and primers gtcatactcaacatctaATTGCGGAAAATGCATGC and catcatcaatatttacaTTATTACGATTTTGTCGCATAAAATTC
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bioRxiv - Microbiology 2021Quote: ... PhyR1 and PhyR2 proteins were overexpressed and purified using the IMPACT kit (New England Biolabs) following the manufacturer’s instructions and equal procedures for the four of them ...
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bioRxiv - Molecular Biology 2021Quote: All constructs were cloned using the NEBuilder HiFi DNA Assembly kit (New England Biolabs E2621) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Total RNA was extracted using the Monarch® Total RNA Miniprep Kit (New England Biolabs) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... coli MG1655 genomic DNA into pKK223-3 by NEBuilder HiFi DNA Assembly Cloning Kit (NEB) and the first 300 bp portion of leuA was translationally fused with the nano-Luc (nLuc ...
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bioRxiv - Microbiology 2021Quote: The pCDNA3.1-hACE2-FLAG plasmid and a Q5 Site-Directed Mutagenesis Kit (New England Biolabs) were used according to the manufacturer’s specifications to generate recombinant plasmids that expressed hACE2 N-glycosylation mutants ...
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Factors required for adhesion of Salmonella enterica serovar Typhimurium to lettuce (Lactuca sativa)bioRxiv - Microbiology 2020Quote: ... Amplified products were reassembled by site-directed mutagenesis (SDM) kit according to manufacturer’s protocol (NEB). Sequence-confirmed plasmids were electroporated in STM SR11 Δ12.
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bioRxiv - Molecular Biology 2021Quote: ... followed by plasmid DNA isolation using the Monarch® Plasmid Minprep kit (New England Biolabs) or a NucleoBond® Xtra Midi kit (Machery-Nagel) ...
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bioRxiv - Molecular Biology 2021Quote: ... PCRs were performed using the Q5® High-Fidelity DNA Polymerase kit (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Specific primers were designed following the instructions of the Q5 site-directed mutagenesis kit (NEB) (primers 35-38 ...
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bioRxiv - Microbiology 2019Quote: ... The reacted DNA was then purified using PCR clean-up kit (New England Biolabs®) and quantified using the nanodrop ...
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bioRxiv - Microbiology 2019Quote: ... sgRNA sequences were cloned using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs). Primers were designed using the NEBaseChanger™ tool (v.1.2.9) ...
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bioRxiv - Microbiology 2020Quote: ... we introduced the desired mutations using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) into pfixK.21 pfixK contains the AU0158 fixLJ sequence along with 670 bp upstream within the pUC18-mini-Tn7-Tp back bone allowing for stable chromosomally integration at an attTn7 site.23,50 Point mutations were verified by Sanger sequencing ...
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bioRxiv - Cancer Biology 2020Quote: ... Libraries were prepared using the NEBNext Ultra Directional RNA Library prep kit (NEB cat # E7420S). Libraries were then sequenced on the Illumina NextSEQ500 ...
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bioRxiv - Cancer Biology 2020Quote: ... Libraries were prepared using the NEBNext Ultra II DNA Library prep kit (NEB cat # E7645). 3 cycles of PCR amplification were used for native inputs ...
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bioRxiv - Cancer Biology 2020Quote: ... with 500ng input RNA or Ultra RNA Library Prep Kit for Illumina (New England Biolabs). An Illumina HiSeq 2500 was utilized to performed paired-end sequencing.