Labshake search
Citations for New England Biolabs :
2851 - 2900 of 3328 citations for 3' Chloro 3 3 chloro 5 fluorophenyl 4' fluoropropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2022Quote: ... The total isolated gDNA was processed in batches of 5 µg per PCR reaction with Q5 polymerase (NEB M0491L). One PCR reaction contained 10 µl 5x reaction buffer ...
-
bioRxiv - Cell Biology 2022Quote: ... the fragment and vector were mixed in a 5:1 ratio and ligated with T4 DNA Ligase (NEB, M0202L) overnight at 16°C ...
-
Atypical epigenetic and small RNA control of transposons in clonally reproducing Spirodela polyrhizabioRxiv - Plant Biology 2024Quote: ... The elution was then ligated to 5′ barcoded RNA adapters using T4 RNA ligase 1 (New England Biolabs, #M0204). To reduce ligation biases ...
-
bioRxiv - Molecular Biology 2023Quote: ... A 5′ adapter (rArCrArCrUrCrUrUrUrCrCrCrUrArCrArCrGrArCrGrCrUrCrUrUrCrCrGrArUrCrU, IDT) was then ligated to RNAs to the product using T4 RNA ligase 1 (NEB) for 4 hours at 15°C ...
-
bioRxiv - Microbiology 2024Quote: ... Plasmid pEC095 was digested with BtsBI and the 5’ overhanging DNA ends were filled in with T4 Polymerase (NEB). A kanamycin resistance marker ...
-
bioRxiv - Cancer Biology 2024Quote: ... RNA was dephosphorylated by incubation with 5 U T4 Polynucleotide Kinase in 1X T4 PNK Buffer (New England BioLabs) supplemented with SUPERaseIn RNase Inhibitor for one hour at 37°C and ligated to bar-coded linkers (NI-810 ...
-
bioRxiv - Genetics 2024Quote: ... DNA digested overnight with XhoI was further digested for ∼5 h with HF-BamHI or NgoMIV (New England Biolabs). Digested DNA was precipitated using standard ethanol/ sodium acetate precipitation.
-
bioRxiv - Molecular Biology 2023Quote: ... DNase-treated RNA (10 µg) was fragmented by using 5 units of ShortCut RNase III (New England Biolabs, M0245) for 5 min at 37°C and the reaction was stopped by adding 10 µL nuclease free water ...
-
bioRxiv - Genetics 2023Quote: ... were ligated overnight at 20°C with a mix of six 5’ phosphorylated telorette oligonucleotides (0.1 µM each) and 2,000 units of T4 ligase (NEB Inc.) in a 50 µl reaction ...
-
bioRxiv - Synthetic Biology 2023Quote: ... 5 µL of the assembly product was used to transform 50 µL of DH5α chemically competent cells (NEB #C2987I) according to the manufacturer’s high-efficiency transformation protocol ...
-
bioRxiv - Genomics 2023Quote: ... A mono-phosphate group was then added back to the 5’-end of RNAs by polynucleotide kinase (PNK, NEB) in the presence of 10mM ATP ...
-
bioRxiv - Developmental Biology 2023Quote: ... cells were pelleted at 500 x g for 5 minutes and resuspended in 8µg/mL recombinant albumin (New England Biolabs) in dPBS-/- ...
-
bioRxiv - Plant Biology 2023Quote: The m6A immunoprecipitation was performed from 5–day–old seedlings using the EpiMark® N6–Methyladenosine Enrichment kit (NEB) starting from 4 μg of total RNA ...
-
bioRxiv - Immunology 2023Quote: ... were single-cell sorted into 5 µl 1% (v/v) Nonidet P40 Substitute, Tris-HCl (20 mM, pH 8.0) containing 5 U murine RNase inhibitor (NEB) using a FACSAriaIII instrument (BD) ...
-
bioRxiv - Developmental Biology 2023Quote: ... was generated from full-length CSN-5 using a Q5 site-directed mutagenesis kit per manufacturer’s instructions (New England BioLabs). Truncated CSN-5 constructs were made by PCR from full-length CSN-5 and inserted into pGEX-KG plasmids ...
-
bioRxiv - Biochemistry 2023Quote: ... A linker RNA oligonucleotides purchased from IDT was preadenylated at 5’ -end by T4 RNA ligase I (NEB 0437) using a method described previously (30) ...
-
bioRxiv - Developmental Biology 2023Quote: ... tissues were then washed twice in DNAse I buffer (66mM Tris pH 7.5 and 5mM MgCl2) for 5 minutes each and then incubated with 10U DNAse (M0303L; New England Biolabs) in 100ul of DNAse buffer at 37 °C for 1hrs ...
-
bioRxiv - Genomics 2023Quote: ... nuclei were sorted into a tube of a PCR tube strip containing 5 μL 1X CutSmart buffer (NEB, B7204S) per well ...
-
bioRxiv - Genetics 2023Quote: ... and F2 crispants were fin clipped at 5 weeks and genotyped by a T7EI assay (M0302, New England Biolabs). We extracted DNA using the Quick-DNA Microprep Kit (D3021 ...
-
bioRxiv - Developmental Biology 2023Quote: ... along with 2.5 ng/μl pCFJ90(Pmyo-2::mCherry) and 77.5 ng/μl of 2-Log DNA ladder (New England BioLabs), to generate two independent strains expressing Ex[Pcol-10::mCherry::his-58::SL2::drl-1 cDNA] (DLS515 ...
-
bioRxiv - Molecular Biology 2023Quote: ... 5 pmol of TP_MAAAPQKCAAA* mRNA (see “Toeprinting assays” section) and components of the PURExpress ΔRibosomes Kit (New England Biolabs). The reaction was incubated at 37ºC for 20 min and then diluted in 50 mM Hepes KOH pH 7.5 ...
-
bioRxiv - Molecular Biology 2023Quote: ... then ligated to a custom 5′ adapter (10 μM oligocap) by T4 RNA Ligase I (M0437, New England Biolabs) with 1 μl RNaseOUT for 16 hours at 16°C ...
-
bioRxiv - Cell Biology 2023Quote: ... genomic DNA was isolated and regions flanking Exon 5 were PCR-amplified using Q5 High-Fidelity DNA Polymerase (NEB) (oligonucleotide primers F ...
-
bioRxiv - Cancer Biology 2023Quote: ... were then denatured at 95°C for 5 min and cooled at room temperature to form heteroduplexes using NEBuffer2.1 1x (NEB). The T7 endonuclease I (NEB ...
-
bioRxiv - Biochemistry 2023Quote: ... The 5’ ends of the CRISPR leader and array fragments were dephosphorylated using Quick Calf Intestinal alkaline Phosphatase (NEB), and the DNAs were purified away from protein using a DNA Clean & Concentrator kit (Zymo Research) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Each library was split in two and transformed separately into 5-alpha cells (NEB, 3.5uL DNA in 35uL cells). Plasmid library isolation ...
-
bioRxiv - Molecular Biology 2023Quote: ... To obtain dephosphorylated TOP2B used for in vitro kinase assay and mass spectrometry the YFP column incubated twice for 15 min at room temperature in wash buffer supplemented with 0.1mM MnCl2 and 5 units mL-1 calf intestinal phosphatase (NEB), 400 units mL-1 lambda protein phosphatase (NEB) ...
-
bioRxiv - Systems Biology 2023Quote: ... with 10 µL RT mix (5 µL 5x Maxima RT Buffer, 1.25 µL 10 mM/each dNTP [New England Biolabs #N0447S] ...
-
bioRxiv - Developmental Biology 2023Quote: ... After centrifugation the EtOH-precipitated fragments were resuspended in water and ligated to 0.25 µM randomized 5’ RNA adapter using T4 RNA ligase 1 (NEB) in the same buffer conditions as described above ...
-
Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration (in COPD)bioRxiv - Cell Biology 2023Quote: ... The quantitative PCR reaction mix contained 5 µl of the Luna Universal qPCR Master Mix (2X, New England BioLabs), 1 µl of the forward primer (10 µM) ...
-
bioRxiv - Biophysics 2023Quote: ... Reaction was terminated with 3 μL of stop buffer (5% sarkosyl, 0.025% bromophenol blue, 50% glycerol) and incubated with 1.6 units of Proteinase K (NEB, P8107S) for 15 minutes at 37 degrees before resolving on 1% agarose TAE gel stained with SybrSafe (Invitrogen ...
-
Recruitment of the m6A/Am demethylase FTO to target RNAs by the telomeric zinc finger protein ZBTB48bioRxiv - Molecular Biology 2024Quote: ... The immunoprecipitated RNA was 5’-end-labeled with 32P using T4 polynucleotide kinase (New England Biolabs catalogue number M0201L). Protein-RNA complexes were separated using 4–12% BisTris–PAGE and transferred to a nitrocellulose membrane (Protran) ...
-
bioRxiv - Biochemistry 2024Quote: ... unlabeled RNAs were dephosphorylated at the 5’-end by treating with calf intestinal alkaline phosphatase (CIP, New England Biolabs) for one hour at 37 °C ...
-
Recruitment of the m6A/Am demethylase FTO to target RNAs by the telomeric zinc finger protein ZBTB48bioRxiv - Molecular Biology 2024Quote: ... The immunoprecipitated RNA was 5’-end-labeled with 32P using T4 polynucleotide kinase (New England Biolabs catalogue number M0201L), separated using 4–12% BisTris–PAGE and transferred to a nitrocellulose membrane (Protran) ...
-
bioRxiv - Cancer Biology 2024Quote: ... was used for tagmentation and amplified with Nextera Ad1_noMX and Ad2.X primers (Supplementary Table 5) using 2x Phusion high-fidelity PCR mix (NEB). AMPure beads (Beckman coulter ...
-
bioRxiv - Developmental Biology 2024Quote: ... along with 2.5 ng/μl pCFJ90(Pmyo-2::mCherry) and 77.5 ng/μl of 2-Log DNA ladder (New England BioLabs), to generate the DLS327 and DLS330 strains ...
-
bioRxiv - Microbiology 2024Quote: ... The RT RNA sample (5 μL) was amplified using the LongAmp Taq 2× Master Mix (NEB, Ipswich, MA, USA) with IVT Nanopore T7 Fw and IVT Nanopore T7 Rv primers (Supplementary Table 5) ...
-
bioRxiv - Molecular Biology 2024Quote: ... 5 rounds of PCR were run using Q5® Hot Start High-Fidelity 2X Master Mix (NEB cat #M0494) following the manufacturer’s instructions with a 63°C annealing temperature and 30s extension ...
-
bioRxiv - Synthetic Biology 2024Quote: ... compatible overhangs for ligation were generated by digestion of PCR products using 5 units each of BsaI (NEB, R3733S) and DpnI (NEB ...
-
bioRxiv - Biochemistry 2024Quote: RP library construction in brief: Footprint RNA was dephosphorylated using 5 U of T4 PNK (New England Biolabs, M0201S). Subsequently ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1.5 μg each of vector pNeae2.1 and insert DNA were combined with 5 μL of 10x T4 Ligase Buffer (NEB), 5 μL of 10x rCutSmart Buffer ...
-
bioRxiv - Immunology 2024Quote: ... 0.5 µl of 2 µM UPA-long and 0.25 µl Phusion Hot Start Flex DNA Polymerase (New England Biolabs) were added to 15.25 µl nuclease-free water ...
-
bioRxiv - Microbiology 2024Quote: ... Next, the DNA adenylated oligonucleotide adenylate intermediate was de-adenylated (RNA sample, NEB Buffer 2, and 5’-deadenylase (NEB); incubation at 30 °C for 1 h ...
-
bioRxiv - Immunology 2024Quote: ... Total RNA samples (5-500 ng) were hybridized with NEBNext rRNA Depletion Kit v2 (Cat# E7400; New England Biolabs) to diminish rRNA from the samples ...
-
bioRxiv - Molecular Biology 2024Quote: ... A circular form of the 3I_RAN FLEXI synthetic oligonucleotide control was generated by incubating the 5’ phosphorylated-3I_RAN FLEXI oligonucleotide (500 ng) with T4 RNA Ligase I (10 U; New England Biolabs) for 2 h at 25°C and 2 min at 95°C ...
-
bioRxiv - Systems Biology 2024Quote: ... complete Protease Inhibitor EDTA-Free) with 5 minutes nutation and then resuspended in tagmentation buffer (1x rCutSmart Buffer NEB #B6004S ...
-
bioRxiv - Genomics 2024Quote: ... uLibs remained unmodified while mLibs were methylated by mixing 250 ng of DNA with 5 µl 1.6 mM SAM (NEB), 5 µl 10x NEBuffer2 and 1 µl M.SssI (NEB ...
-
bioRxiv - Biochemistry 2024Quote: The 9N_VRA3 adapter oligonucleotide (0139, Supplementary Table 2) was pre-adenylated with the 5’ DNA Adenylation Kit (New England Biolabs) using the following protocol ...
-
bioRxiv - Synthetic Biology 2024Quote: ... 1–5 μg of plasmid was linearized prior to transformation using either ScaI-HF or EcoRI-HF (both NEB), respectively ...
-
bioRxiv - Genomics 2024Quote: ... we performed PCR2 reactions (10,000gRNAs per 50ul reaction) using 5 ul of the pooled PCR1 product per reaction with Q5 polymerase (NEB). Each biological sample was amplified with PCR2 primers with unique barcodes (Supplementary Table 1) ...