Labshake search
Citations for New England Biolabs :
2601 - 2650 of 4208 citations for Huntingtin Interacting Protein 1 Related Protein HIP1R Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2022Quote: ... 5’ adaptor ligation using T4 RNA Ligase 1 (New England Biolabs, Ipswich, MA, M0204L). Generation of cDNA was done by using Superscript III Reverse Transcriptase (Life Technologies ...
-
bioRxiv - Microbiology 2022Quote: ... and 1 μl T4 RNA ligase 2 (truncated K227Q, NEB, M0242, 200,000 units/ml) and incubated at 16°C for 14 h ...
-
bioRxiv - Microbiology 2022Quote: Fragmented gDNA (1 μg) was end-repaired using an End-repair module (NEB#E6050), and dA-tailed using Klenow (NEB#6053) ...
-
bioRxiv - Molecular Biology 2019Quote: ... Ligation reaction proceeded by addition of T4 RNA Ligase 1 (New England Biolabs, M0437M) at 23°C for 75 minutes ...
-
Imaging of Morphological and Biochemical Hallmarks of Apoptosis with Optimized Optogenetic ActuatorsbioRxiv - Biochemistry 2019Quote: The Cry2(1-531) gene was amplified by polymerase chain reaction (NEB Q5 polymerase) from the full length Cryptochrome-2 gene using a forward primer encoding an XhoI-restriction site with the sequence ...
-
bioRxiv - Genomics 2019Quote: ... 1 × SYBR Green and 5μl NEBNext High-Fidelity 2X PCR Master Mix (NEB M0541S), to estimate the optimum number of enrichment cycles with the following program ...
-
bioRxiv - Genetics 2020Quote: ... remaining ssDNA oligonucleotides were digested by the addition of 3 μL exonuclease 1 (NEB) to 20 μL extracted DNA ...
-
bioRxiv - Synthetic Biology 2019Quote: ... using 1 µL of each sample or mixed with 1x DNA loading dye (NEB) and loaded on a 10 % native polyacrylamide gel (PAGE ...
-
bioRxiv - Synthetic Biology 2020Quote: ... we cooled the samples to 37C and added 20U of Exonuclease 1 (NEB, M0293S) to the reaction to remove excess primers ...
-
bioRxiv - Microbiology 2019Quote: ... Incubation for 1 h at 55°C with 8U of proteinase K (NEB P8107S) finally disrupted viral capsids ...
-
bioRxiv - Developmental Biology 2020Quote: ... Rehybridized product was digested with 1 μL T7 endonuclease I enzyme (New England Biolabs) at 37°C for 1 hour and separated on a 1.5% agarose gel ...
-
bioRxiv - Molecular Biology 2020Quote: ... Resulting PCR amplicons were denatured and re-annealed in 1 × NEB buffer 2 (NEB) in a total volume of 9 μl using a following conditions ...
-
bioRxiv - Molecular Biology 2021Quote: ... The fragmented RNA was then purified and circularized with T4 RNA ligase 1 (NEB). Tandem repeats were generated during first-strand synthesis at 42 °C for 30 min ...
-
bioRxiv - Bioengineering 2019Quote: ... the culture media mix was TSB with 1 mg/mL BSA (New England Biolabs) and 50 μM calcein ...
-
bioRxiv - Synthetic Biology 2019Quote: All digestions were performed for 1-2h at 37° with REs purchased from NEB, and purified using the Monarch PCR & DNA Cleanup Kit (NEB) ...
-
bioRxiv - Genomics 2019Quote: ... in 1× Buffer 3 (Bionano Genomics) or 120 U of Nb.BssSI (New England Biolabs) in 1× NEBuffer 3.1 ...
-
bioRxiv - Genetics 2020Quote: ... and 0.3 μL quick ligase in 1 x quick ligase buffer (New England Biolabs) with a total volume of 6 μL ...
-
bioRxiv - Microbiology 2021Quote: ... After incubation with 5‘-phosphate-dependent exoribonuclease XRN-1 (New England BioLabs, Inc., USA), samples were treated with RNA 5‘ polyphosphatase (Lucigen) ...
-
Regulation of skeletal muscle metabolism and contraction performance via teneurin-latrophilin actionbioRxiv - Physiology 2021Quote: ... 1 μL deoxynucleotide Solution Mix and 0.5 μL Taq DNA Polymerase (New England Biolabs). The reactions were incubated in an Eppendorf Mastercycler Gradient Thermal Cycler for 7m at 95°C ...
-
bioRxiv - Biochemistry 2021Quote: ... one of which was incubated with 1 µl nicking enzyme (10 units Nt.BspQI (NEB) or 5 units Nb.Bpu101 (ThermoFisher) ...
-
bioRxiv - Biochemistry 2021Quote: ... reaction products were resuspended in 50 µl relevant manufacturer’s enzyme buffer (1 × Cutsmart (NEB) for nicking with Nt.BspQI or 1 × Buffer R (ThermoFisher ...
-
bioRxiv - Biochemistry 2021Quote: ... and 0.02% NP-40) including 1 μL of PstI (NEB, at 100,000 U/mL), 2 mM ATP (Sigma-Aldrich) ...
-
bioRxiv - Cell Biology 2021Quote: ... and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1) (NEB E7335) according to the manufacturer’s protocols ...
-
bioRxiv - Molecular Biology 2020Quote: ... The chambers of the microplate were pre-treated with 1 mg/ml BSA (NEB) for 30 min before aspirating the BSA ...
-
bioRxiv - Immunology 2020Quote: ... enzymatic digestion was performed 30 min with 1 unit T7 endonuclease (New England Biolabs) at 37 °C ...
-
bioRxiv - Immunology 2021Quote: ... Digestion was performed for 1 h using Cut Smart Buffer (NEB, Cat no. B7204S). Next ...
-
bioRxiv - Biophysics 2020Quote: ... The flow cell was incubated for 10 minutes with 1 mg/ml streptavidin (NEB) and then 10 minutes with Blockaid (ThermoFisher) ...
-
bioRxiv - Bioengineering 2022Quote: ... Each 10 µL experiment contained: 1 µL 10x isothermal buffer (New England Biolabs (NEB)) ...
-
bioRxiv - Bioengineering 2022Quote: ... Each 10 µL experiment contained: 1 µL 10x isothermal buffer (New England Biolabs (NEB)) ...
-
bioRxiv - Biophysics 2022Quote: ... fluorescently labeled dCas9 was incubated with gRNA in 1× NEBuffer 3.1 (New England Biolabs). 1 nM dCas9 was incubated with 10 nM gRNA for 30 min at 37 °C on an orbital shaker ...
-
bioRxiv - Bioengineering 2022Quote: ... we used a 1 kb Plus DNA Ladder for Safe Stains (NEB; catalog # N0559S), Gel Loading Dye ...
-
bioRxiv - Microbiology 2022Quote: ... two complementary oligonucleotides (1 μM each) were hybridized in T4 DNA-ligase-buffer (NEB) containing T4 polynucleotide kinase (PNK ...
-
bioRxiv - Microbiology 2022Quote: ... To 200 μL of supernatant 1 μL DNase (New England Biolabs, catalog number: M0303S) 1 μL RNaseA (Biomed ...
-
bioRxiv - Synthetic Biology 2022Quote: ... and assembled using NEBuilder HiFi DNA Assembly Master Mix (NEB, 1 h 50° C) into backbones previously digested with corresponding restriction enzymes (NEB ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... (1) DNA fragments were repaired with the End-Repair enzyme mix (New England Biolabs). (2 ...
-
bioRxiv - Biophysics 2022Quote: ... diluted to ∼1 nM in 1x T4 DNA ligase reaction buffer (New England Biolabs), and treated with T4 DNA ligase to circularize the DNA ...
-
bioRxiv - Cell Biology 2024Quote: ... 0.1% BSA) with or without Short Cut RNase III (New England Biolabs; 1:150) for 90 min at 37°C ...
-
bioRxiv - Cell Biology 2024Quote: ... cells were treated with a 1:150 dilution of RNase III (New England Biolabs) in low-salt buffer (50 mM Tris-HCl pH 7.6 ...
-
bioRxiv - Biophysics 2023Quote: 12mer arrays (40 ng) were digested with 1 U of MNase (New England Biolabs) in a 10 μL reaction for varying digestion times (2.5 to 10 minutes ...
-
bioRxiv - Microbiology 2024Quote: ... Cells were harvested and resuspended with 1:10 volumes of CutSmart Buffer (NEB, # B7204) and 5 µl of 100 mg/ml RNase-A solution (pre-heated at 100°C for 20 minutes) ...
-
bioRxiv - Genetics 2024Quote: ... immunoprecipitated RNA and 1% input RNA Monarch RNA Cleanup Kit (New England Biolabs, T2047L) was used according to the manufacturers’ instructions ...
-
bioRxiv - Genomics 2024Quote: ... 1 μg of the step one plasmid library was linearized with XhoI (NEB #R0146) at 37°C for 16 hours ...
-
bioRxiv - Genomics 2024Quote: ... The digested nuclei were washed and resuspended by 1 x T4 ligase buffer (NEB). Nuclei were then ligated for 4 h at room temperature by adding 1% Triton X-100 ...
-
bioRxiv - Immunology 2024Quote: ... 1 µl per 50 µl pool volume of Thermolabile Exonuclease I (New England Biolabs) was then added and incubated for 4 minutes at 37 °C ...
-
bioRxiv - Genomics 2023Quote: ... a dual-enSERT-2.1 plasmid (1 μg) was digested overnight with PauI (NEB, R0199) at 50°C in rCutSmart Buffer ...
-
bioRxiv - Genomics 2023Quote: ... and NEBNext® Multiplex Oligos for Illumina® Index Primers Set 1 (NEB# E6440). The generated DNA libraries were purified using Agencourt® AMPure® XP (Beckman Coulter) ...
-
bioRxiv - Microbiology 2023Quote: ... 1 μg RNA was reverse transcribed to cDNA using LunaScript RT SuperMix Kit (NEB). qPCR was performed in 20 μl reactions containing diluted cDNA ...
-
bioRxiv - Molecular Biology 2023Quote: ... these products were ligated to a 3’ adapter (1× T4 RNA ligase buffer (NEB), 10% PEG-8000 ...
-
bioRxiv - Molecular Biology 2023Quote: ... this was followed by incubation with 1 µl PNGase F (NEB, Ipswich, MA, USA), 2 µl 10% NP-40 (NEB ...
-
bioRxiv - Microbiology 2023Quote: ... For XRN1 treatment refolded RNA (1 μg) was incubated with 1U XRN1 (NEB, USA) and 10U RppH (NEB ...