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Citations for New England Biolabs :
2501 - 2550 of 4208 citations for Huntingtin Interacting Protein 1 Related Protein HIP1R Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... After the ligation of adaptors using T4 RNA ligase 1 (New England Biolabs), reverse transcription was executed ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCR-1 was carried out using 2X Taq polymerase mastermix (New England BioLabs) while PCR-2 and PCR-3 were carried out using dATP ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 1 μg of total RNA was treated with amplification-grade DNase I (NEB) and reverse transcribed using Oligo(dT)20 as a primer and Superscript III Reverse Transcriptase (Life Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was circularized using T4 RNA ligase 1 (Epicentre or New England BioLabs) and reverse transcribed using Superscript III (Invitrogen ...
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bioRxiv - Genetics 2022Quote: ... was digested for 1 h with 50 units of DpnI (New England Biolabs) to eliminate unreplicated plasmids ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 hour at 37 °C and SfiI (New England Biolabs, catalog #R0123) for 5 hours at 50 °C ...
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bioRxiv - Microbiology 2023Quote: ... 1 unit of Phusion Polymerase were mixed with 1x HF Phusion buffer (NEB) and made up to 50 µl total volume with H20 ...
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bioRxiv - Plant Biology 2022Quote: ... Of each entry plasmid 100 ng was combined with 1× Cutsmart buffer (NEB), 1 mM ATP ...
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bioRxiv - Systems Biology 2023Quote: ... NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) oligos (NEB EE7600S), and an Illumina NextSeq 550 75-cycle high-output kit (Illumina #20024906) ...
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bioRxiv - Synthetic Biology 2022Quote: ... and 1 µl of a dual unique index multiplex primer pair (NEB; E6440) in a total reaction volume of 25 µl ...
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bioRxiv - Synthetic Biology 2022Quote: ... into backbones previously digested with corresponding restriction enzymes (NEB, 1 h 37° C) to yield intermediate plasmids containing individual transcriptional units ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 µL was used in a 10 µL Kinase/Ligase/DpnI (KLD, NEB) reaction and incubated at room temperature for 10-20 min ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 μg BSA per well dealt as negative control (NEB, Ipswich, MA, USA). Samples were applied in a final volume of 100 μl coating buffer (100 mM Tris-HCL pH 8 ...
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bioRxiv - Systems Biology 2024Quote: ... and 1 µl of Klenow large fragment DNA polymerase (New England Biolabs M0210) were then added ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 ul of 10x Template Switching Reverse Transcriptase (New England Biolabs, MA, USA) for the synthesis of the 1st strand ...
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bioRxiv - Molecular Biology 2024Quote: ... Adaptor ligation was performed with a 1:10 dilution of adaptor (NEB, E6440S). For DNA purification ...
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bioRxiv - Neuroscience 2024Quote: ... the construct was diluted 1:5 with empty pUC19 vector (New England Biolabs) and then transfected with 7.5 µL of TransIT-293 and 2.5 µg of DNA ...
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bioRxiv - Cell Biology 2024Quote: ... and/or 1 µM SiR-647-SNAP-dye (New England Biolabs, Waltham, MA) or 100 nM DAPI (Life Technologies) ...
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bioRxiv - Developmental Biology 2024Quote: ... Nuclei were taken up in 520 µl of 1× DpnII buffer (NEB, R0543M) and incubated with 7.5 µl of 20% SDS for 1 hour shaking at 600 r.p.m ...
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bioRxiv - Genetics 2024Quote: ... for 1 hour and then treated with 1.6 units of Proteinase K (NEB) for a further hour before cleanup of DNA with AMPure XP Beads (Beckman Coulter ...
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bioRxiv - Microbiology 2023Quote: ... and 1 μl T4 RNA ligase 2 (truncated K227Q, NEB, 200,000 units/ml) and incubated at 16°C for 14 h ...
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bioRxiv - Cancer Biology 2023Quote: ... NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) oligos (NEB E7600S) was used for assigning indices to all samples prior to pooling ...
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bioRxiv - Immunology 2023Quote: All PD-1 mutants were generated using Q5 site-directed mutagenesis kit (NEB) following the manufacture’s protocol ...
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bioRxiv - Genomics 2023Quote: ... and Uracil-Specific Excision Reagent (1 U/μl USER, catalog num. M5505L, NEB) treatment ...
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bioRxiv - Microbiology 2023Quote: 1 µg of total RNA was treated with DNase-I (New England Biolabs) followed by cDNA synthesis using iScript cDNA synthesis kit (Bio-rad ...
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bioRxiv - Molecular Biology 2023Quote: ... the extracted cDNA was then added to 1 x Terminal Transferase buffer (NEB), 250 µM CoCl2 (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... The clarified lysate is mixed with 1 mL of amylose resin (E8021S, NEB) pre-equilibrated with lysis buffer and incubated in the cold room using an end-over-end rotor for 2 hours ...
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bioRxiv - Immunology 2023Quote: ... The 3lll adaptor was added using T4 RNA ligase 1 (New England Biolabs) for 2.5 hours at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... The final Golden Gate reaction volume contained 1× concentrated T4 ligase buffer (NEB), 250 ng Golden Gate vector (pcDNA3-based carrying a twin Strep-FLAG tag ...
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bioRxiv - Genetics 2023Quote: ... the rRNA was eliminated by adding 1 μl RNase H (New England Biolabs), 4 μl 10X RNase H buffer ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% Triton X-100) supplemented with Proteinase K (1:100, New England Biolabs). Specimens were incubated on an orbital shaker at 37°C per 2 hours and 60 rpm.
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bioRxiv - Biophysics 2023Quote: ... The left lane (“L”) has a DNA size ladder (1 kb+, NE Biolabs). The black arrow in the left lane (“P” ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was first circularized by a T4 RNA Ligase 1 Kit (M0204. NEB) and then purified by TRIzol reagent followed by isopropanol precipitation ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and the NEBNext Multiplex Oligos for Illumina (Index Primers Set 1) by NEB following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... with NEBNext Multiplex Oligos for Illumina Dual Index Primers Set 1 (NEB, #E7600S) with primer indexing PCR run according to the Epicypher protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 μl were combined with 1 μl 10X T4 Ligation Buffer (NEB, M0202), 6.5 μl Nuclease-free H2O and 0.5 μl T4 Polynucleotide Kinase (PNK ...
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bioRxiv - Microbiology 2023Quote: ... RNA concentration was determined by nanodrop and DNase 1 treated (NEB, Cat. # M0303L). DNase 1 (4 units ...
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bioRxiv - Developmental Biology 2023Quote: ... 1μg RNA was incubated with either 1 unit of XRN1 (New England Biolabs) or water for 2hr at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... 4 ng gBlock template and 1 Unit Phusion High-Fidelity DNA polymerase (NEB). The reaction was subjected to a 2-minute initial denaturation at 98 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... were ligated using NEBNext DNA Sample Prep Reagent Set 1 (New England Biolabs). Immunoprecipitations were carried out using 500ng DNA per sample ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μl of 10x T4 RNA ligase 2 (truncated) buffer (New England Biolabs), 3 μl of 50% PEG 8,000 (New England Biolabs) ...
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bioRxiv - Biochemistry 2023Quote: ... Oligo 1 and oligo 2 were annealed by T4 polynucleotide kinase (NEB, #M0201S) at 37 ℃ for 30 min and 95 ℃ for 5 min ...
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bioRxiv - Molecular Biology 2023Quote: ... sgCD81i-1 g[9nt] and g[8nt] guides required golden gate assembly (NEB) due to the short length of the oligonucleotides ...
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bioRxiv - Genomics 2023Quote: ... Nuclei were then digested with DpnII (1:50) (NEB, 50 000 U/ml) for 4h at 37°C under agitation ...
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bioRxiv - Genomics 2023Quote: ... 160 µL of 40 U µL-1 Taq DNA ligase (New England Biolabs) and 700 µL of PCR-grade H2O ...
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bioRxiv - Molecular Biology 2023Quote: ... 39 µL of qPCR mix containing 1× Phusion mix (New England Biolabs, M0531S), 0.125 µM P6 Tall v4 primer ...
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bioRxiv - Genetics 2023Quote: ... PCR-1 and PCR-2 amplicons were digested with DpnI (NEB Cat#R0176S) for 2 hours at 37°C to remove the YCp50-WT_PKR template ...
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bioRxiv - Genomics 2023Quote: ... were added to the reaction with T4 DNA Polymerase (1 unit; NEB, M0203) and incubated at 12°C for 15 min ...
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bioRxiv - Genomics 2023Quote: ... Beads were re-suspended in 30μl of 1 × NEB Buffer2 (NEB Cat#7002S) containing 9U of T4 DNA polymerase (NEB ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and treated with 1 µL (20 units) of DpnI (New England Biolabs, R0176L) for 1 h at 37°C before being purified on magnetic beads ...