Labshake search
Citations for New England Biolabs :
2551 - 2600 of 4333 citations for 2H Pyrazolo 4 3 c pyridine 3 chloro 4 5 6 7 tetrahydro since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... NEB 5-alpha competent E.coli (NEB #C2987H, Ipswich, MA, USA) were transformed with ligated plasmid by heat shock at 42°C for 30 seconds ...
-
bioRxiv - Cancer Biology 2023Quote: ... Linker reactions were removed with 5′ deadenylase (New England Biolabs) and Rec J Exonuclease (NEB) ...
-
bioRxiv - Physiology 2024Quote: ... 5 μL of α1-2,4,6 fucosidase O (2U/μl, NEB) with 1x GlycoBuffer 1 (NEB).
-
bioRxiv - Plant Biology 2024Quote: ... 5 and 7dpi using a total RNA extraction kit (NEB) and following the kit instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... Five μl of 5 U/μl Klenow fragment (NEB M0210L) was added to achieve a final concentration of 0.5 U/μl and incubated at 37°C for 15 min with the same agitation program as the previous step ...
-
bioRxiv - Molecular Biology 2024Quote: ... 5 μL T4 Polynucleotide Kinase Reaction Buffer (NEB, Cat #B0201S) and 5 μL 10 mM ATP ...
-
bioRxiv - Genomics 2024Quote: ... 1 μL BST DNA Polymerase FL (NEB, 5 U/µL), 2μL TAQ Ligase (NEB ...
-
bioRxiv - Immunology 2024Quote: ... and 5 μL of Proteinase K (NEB catalog no. P8107S). Tubes were incubated for 15 min at 37 °C with shaking at 600 rpm ...
-
bioRxiv - Microbiology 2024Quote: ... 5 μl inorganic pyrophosphate (New England Biolabs product number M2403), and ultrapure water (as needed ...
-
bioRxiv - Genomics 2024Quote: ... and dTTP with 5 U/µL DNA polymerase (NEB # M0210), the DNA was ligated by T4 DNA ligase (NEB # M0202 ...
-
bioRxiv - Systems Biology 2024Quote: ... 1 μL of Klenow DNA Polymerase (5 U/μL, NEB) and 2 μL of T4 PNK (10 U/μL ...
-
bioRxiv - Genomics 2024Quote: ... and 5 µl DNA Polymerase I (Large Klenow Fragment) (NEB). The reaction was incubated for 60 min at 37 °C and subsequently purified and eluted in 13 µl ddH2O using a MinElute kit (Qiagen ...
-
bioRxiv - Microbiology 2024Quote: Chemically competent NEB® 5-alpha F’Iq cells (NEB; C2992H) were transformed with recombinant plasmids per manufacturer instructions and grown at 37°C on selective Luria-Bertani (LB ...
-
bioRxiv - Bioengineering 2024Quote: ... 5’-end labeling was performed using T4 Polynucleotide Kinase (NEB) and [gamma-32P] ATP (PerkinElmer) ...
-
bioRxiv - Bioengineering 2024Quote: ... 5 μL of T4 DNA ligase (NEB, 400000 U/mL), and 10 μL of 10X T4 DNA ligase buffer (NEB) ...
-
bioRxiv - Cancer Biology 2024Quote: ... 5 μl H2O and 1 μl PNGase F (NEB, P0704S) were then added to the lysate ...
-
bioRxiv - Bioengineering 2024Quote: ... then 5 μL of 6X Purple Loading Dye (NEB, B7024S) were added and run on a GelRed-stained 1% (w/v ...
-
bioRxiv - Genetics 2021Quote: ... EagI/KpnI-digested ORFs were ligated into the EagI/KpnI-digested pUASTattB vector backbone using a 6:1 insert:vector molar ratio and T4 DNA ligase (M0202S, NEB) in a thermocycler overnight (∼16 hr) ...
-
bioRxiv - Molecular Biology 2020Quote: ... 50 µl of 1xMNase buffer containing 60 gel units/ml (6 Kunz units/ml) of Micrococcal nuclease (NEB M0247S) were added and incubated for exactly for 5 min ...
-
bioRxiv - Synthetic Biology 2020Quote: ... and subsequently mixed at a 6:1 ratio with the digested backbone in reactions containing T4 DNA ligase (NEB).
-
bioRxiv - Developmental Biology 2020Quote: ... and 6 cDNA libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) with 9 amplification cycles ...
-
bioRxiv - Biophysics 2021Quote: Polymerised gels were removed from the coverslip chamber and placed into 6-well plates to undergo digestion in 0.2 mg/ml proteinase K (New England Biolabs) dissolved in digestion buffer (50 mM Tris pH 8.0 (Thermo Fisher Scientific) ...
-
bioRxiv - Plant Biology 2020Quote: ... the supernatant was passed twice at a flow rate of 0.5 mL/min through a column with 6 mL chitin beads (NEB) equilibrated with lysis buffer ...
-
bioRxiv - Biochemistry 2022Quote: ... 6 μM kifunensine was added to the ExpiCHO culture media at the time of transfection and Endo Hf (NEB) treatment of LC-kappa purified protein (∼10,000 U/mg protein at 4°C for 3 hours ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... see Supplementary Data 6) and cloned into pDHFR vector provided in the PURExpress In Vitro Protein Synthesis Kit (NEB). The translation into liposomes was done as described previously95 and the output was analyzed by Blue Native PAGE using 2% digitonin and NativePAGE Novex 4-16% Bis-Tris Protein Gel (Thermo Fisher Scientific) ...
-
bioRxiv - Genetics 2020Quote: ... A 6 µL mixture containing 2 µL of 40 µM Spy Cas9 NLS protein (New England Biolabs, MA, USA), 200 ng each of five sgRNAs (in 2 µL ...
-
bioRxiv - Biochemistry 2021Quote: To generate circular DNA with a single nick phagemid DNA (~2,6 μg) with or without non-denaturing bisulfite treatment was treated with 20 Units of Nt.BstNBI (New England Biolabs) for 1 hour at 37°C in 50 μl of 1X NEBuffer 3.1 followed by deactivation at 70°C for twenty minutes.
-
bioRxiv - Molecular Biology 2023Quote: ... The cDNA was amplified in a 25 µl PCR reactions using 6 µl cDNA and 2X Master mix (NEB). The PCR cycling program was 98 °C for 30 seconds ...
-
bioRxiv - Genomics 2023Quote: ... DNA was extracted from 4.3×10^6 cells using the Monarch HMW DNA Extraction Kit for Cells and Blood (New England Biolabs) with 1400 rpm lysis speed ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 8 μL of solution A and 6 μL of solution B were mixed with 16 U RNase Inhibitor (NEB), gBlock templates (2 nM each ...
-
bioRxiv - Cell Biology 2022Quote: ... Cx36-SNAP transfectants were treated with 6 µM SNAP-Cell Oregon Green (New England Biolabs Inc., Boston, MA, USA) in 10% FBS DMEM for 30 min at 37°C to quench all Cx36-SNAP molecules ...
-
bioRxiv - Biochemistry 2023Quote: 3 μL of the transcription and co-transcriptional reactions were added to a 6 μL mixture containing 1x DNase I reaction buffer and 0.5 U/μL DNase I (New England Biolabs). After incubation at 37°C for 30 min ...
-
bioRxiv - Molecular Biology 2023Quote: ... 15 µL 100 µM dNTPs, 0.3 µL 50 mg/mL BSA, 6 µL blunting enzyme mix from quick blunting kit, NEB). Prior to ligation ...
-
bioRxiv - Microbiology 2024Quote: ... Reactions were then DpnI-treated for 6 hours and purified using the Monarch PCR and DNA Cleanup Kit (NEB) according to the manufacturer’s protocol ...
-
bioRxiv - Biophysics 2024Quote: ... 800 μL of 2 M MgCl2 and 1.6 mL 1 M CaCl2 were added to the lysed cells along with 2.5 μL DNase (New England Biolabs) and OmniCleave endonuclease (Biosearch Technology ...
-
bioRxiv - Biophysics 2024Quote: ... the eluate from IgG Sepharose 6 FF was incubated with SNAP-Surface-Alexa Flour 647 (New England Biolabs, S9136S) at 4 °C overnight ...
-
bioRxiv - Cell Biology 2020Quote: ... 800ng were treated for 20’ at 37°C with 50u ShortCut Rnase III (NEB), in the digestion buffer supplied by the manufacturer ...
-
bioRxiv - Developmental Biology 2021Quote: ... and 2 hours at 55°C with 0.1 mg/ml Proteinase K (NEB P8107S).
-
bioRxiv - Molecular Biology 2020Quote: ... digested overnight at 50°C with 1 μl 20 U/μl SfiI (NEB R0123S) in 200 μl 1x CutSmart buffer ...
-
bioRxiv - Molecular Biology 2020Quote: ... digested overnight at 50°C with 1 μl 20 U/μl SfiI (NEB R0123S) then rinsed with 1xTE.
-
bioRxiv - Molecular Biology 2021Quote: ... Ligation was conducted overnight at 16 °C with T4 DNA Ligase (New England Biolabs). All minigene mutations were introduced via Q5 Site-Directed Mutagenesis Kit (New England Biolabs) ...
-
bioRxiv - Genomics 2020Quote: ... with BspQ1 overhangs were ligated using T7 DNA ligase (NEB M0318; 16°C overnight) into BspQ1 digested (NEB R0712 ...
-
bioRxiv - Plant Biology 2021Quote: ... Overnight digestion at 37 °C was performed using 400U of Hind III enzyme (NEB). Digested DNA was ligated during 5 h incubation at 16 °C with 100 U of T4 DNA ligase (NEB) ...
-
bioRxiv - Cancer Biology 2021Quote: ... 40 μg of Hi-C library DNA were incubated with T4 DNA polymerase (NEB) for 4 hours at 20°C to remove of biotin from non-ligated fragment ends ...
-
bioRxiv - Systems Biology 2020Quote: ... The resulting PCR products were digested for 1h at 37°C with DpnI (NEB) and transformed in competent Escherichia coli MC1061 cells ...
-
bioRxiv - Microbiology 2021Quote: ... the samples were incubated at 37°C overnight with PNGase F (New England BioLabs) to release the glycans ...
-
bioRxiv - Genomics 2021Quote: ... and 6h hours at 37°C with 20 U of MspI (New England Biolabs) in 30 μl of 1x NEBuffer 2 ...
-
bioRxiv - Microbiology 2021Quote: ... gDNA was digested overnight (37°C) with the restriction enzyme RsaI (New England Biolabs) to produce small ...
-
bioRxiv - Immunology 2022Quote: ... and 789-203-3C12 IgG was digested with endoproteinase Lys-C (New England Biolabs) overnight at room temperature ...
-
bioRxiv - Molecular Biology 2022Quote: ... and ligated at 16 °C with 10,000 U of T4 DNA ligase (NEB, M0202) in ligase buffer supplemented with 0.8% Triton X-100 (Sigma-Aldrich ...