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Citations for New England Biolabs :
2501 - 2550 of 4045 citations for 7 11 Dimethyldodeca 4 6 10 trien 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... and 10 μL 5U/μL DNA Pol I Large Klenow fragment (NEB), then incubated at 37 °C for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: ... the protein was batch bound to 10 mL of chitin resin (NEB) for 1 hour at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Ligation master mix (398 μL of water, 50 μL of 10 × NEB T4 DNA ligase buffer ...
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bioRxiv - Synthetic Biology 2021Quote: ... qPCR reactions (10 µL each) were performed with OneTaq polymerase (NEB #M0484) in 96 well PCR plate (4titude 4ti-0750) ...
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bioRxiv - Genetics 2021Quote: ... 10 µM dNTP and 0.05 U Taq DNA polymerase (New England Biolabs). An initial denaturation step at 97°C for 2 minutes was followed by 45 cycles of 10 seconds at 95°C ...
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bioRxiv - Genomics 2021Quote: DNA end modification: 10 U T4 Polynucleotide Kinase (New England Biolabs, M0201) in 60 μl 1×T4 DNA Ligase reaction buffer were added to each sample and incubated at 37°C for 30 minutes.
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bioRxiv - Genomics 2020Quote: ... concentrated 4x and transformed in electrocompetent E.coli cells (10-beta Electrocompetent, NEB). Cells were recovered in SOC medium and plated on LB with Ampicillin ...
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bioRxiv - Genomics 2020Quote: ... 10 mM ATP and 50% (w/v) PEG8000 (NEB cat. no. M0204S)
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bioRxiv - Microbiology 2020Quote: ... 10 μL of 2x Hot Start Taq master mix (New England BioLabs) and 8.18 μL of water (= 20 μL total) ...
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bioRxiv - Genomics 2021Quote: ... 0.5 μL of 10 mM Deoxynucleotide (dNTP) solution mix (New England Biolabs) was added to each reaction and nuclease -free water was used to make up the remaining reaction volume ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and 0.2 μl of AccI enzyme (10 U/mL, New England Biolabs). Restriction profiles specific to each species (Scer or Suva ...
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bioRxiv - Microbiology 2024Quote: ... beads were incubated with 10 μM SNAP-Surface Alexa Fluor 488 (NEB) for 1 hour at 4°C prior to elution ...
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bioRxiv - Neuroscience 2022Quote: ... 10 ng genomic DNA was digested with 30 units of MspI (NEB). Fragments were ligated to pre-annealed adapters containing 5’-methyl-cytosine instead of cytosine according to Illumina’s specified guidelines ...
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bioRxiv - Molecular Biology 2022Quote: ... Correct constructs were re-transformed into 10-beta chemically competent cells (NEB), plated on LB agar plates containing chloramphenicol ...
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bioRxiv - Biochemistry 2024Quote: ... Correct constructs were re-transformed into 10-beta chemically competent cells (NEB), from which clones were used to prepare glycerol stocks (25% glycerol ...
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bioRxiv - Molecular Biology 2024Quote: ... Beads were treated with 10 μl of T4 Polynucleotide kinase (PNK; NEB) in 100 μl of 1X T4 PNK Reaction buffer containing 1 U/μl SUPERase•In™ RNase Inhibitor at 37°C for 30 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... were added with 1.5 µL of 10× GlycoBuffer 1 (New England Biolabs) in the sample by adjusting with Milli-Q H2O to total 15 µL ...
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bioRxiv - Genomics 2024Quote: ... and dephosphorylated 10 minutes at 37°C using Quick CIP (NEB, M0525). The vectors were then purified with PCR CleanUp kit (Macherey Nagel ...
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bioRxiv - Immunology 2024Quote: ... with 10 × DNase I buffer diluted in water (New England Biolabs #M0303S). Samples were incubated in the thermocycler for 30 minutes at 37°C ...
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bioRxiv - Immunology 2023Quote: ... 2 μL of T4 RNA Ligase (10 000 U/mL, NEB, M0204S), and 10 μL of PEG 8000 50% (NEB ...
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bioRxiv - Bioengineering 2022Quote: ... and 0.5 µL 10 U/µL DNase I (New England Biolabs, M0303L) incubate for 30 min at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... 2 μg 5’PPP transcripts were incubated with 10 U RppH (NEB) and 20 U RiboLock (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... 10 μl of Q5® reaction buffer (5X; New England Biolabs, UK), 0.5 μl of deoxynucleotide (dNTP ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 µl of each sample was digested with 20 U XhoI (NEB) for 3-6 hours in 20 μl 1x CutSmart buffer (NEB) ...
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bioRxiv - Cell Biology 2023Quote: ... 5 µL of 10× rCutSmart™ Buffer (New England BioLabs, Ipswich, MA), 10 units each of SalI and EcoRV restriction enzymes (New England BioLabs ...
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bioRxiv - Cell Biology 2023Quote: ... 10 units each of SalI and EcoRV restriction enzymes (New England BioLabs) topped up to 50 µL with nuclease-free water ...
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bioRxiv - Molecular Biology 2023Quote: ... was followed by cleavage with 10 U BspHI (New England Biolabs, #R0517S) and incubated at 37°C for 20 min followed by dephosphorylation with 1 U Shrimp Alkaline Phosphatase (rSAP ...
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bioRxiv - Synthetic Biology 2023Quote: ... Restriction digest using BsmBI v2 (NEB, stock concentration 10 000 U/mL) was performed at a BsmBI-v2 concentration of 0.67 U/μL ...
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bioRxiv - Genomics 2023Quote: ... and 2 µl CutSmart buffer (New England Biolabs, cat. no. B7204S, 10×) at 37°C for 2h ...
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bioRxiv - Microbiology 2023Quote: ... by incubating with 10 U of T4 polynucleotide kinase (New England Biolabs) at 37°C for 1 h.
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bioRxiv - Molecular Biology 2023Quote: ... and then transformed into NEB 10-beta competent cells (New England BioLabs). Plasmid DNA was extracted from ten positive clones using the GeneJET Plasmid Miniprep Kit (Thermo Scientific ...
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bioRxiv - Genomics 2023Quote: ... 10 µL of 400 U/µL T4 DNA Ligase (Cat#M0202, NEB), and 562 µL of water was added ...
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bioRxiv - Cell Biology 2023Quote: ... 10 µL Q5® High GC Enhancer (New England BioLabs, Inc., B9028AVIAL), 0.5 µL Q5® High-Fidelity DNA Polymerase (New England BioLabs ...
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bioRxiv - Biophysics 2022Quote: ... and 10 units of T4-polynucleotide kinase (New England BioLabs, Ipswich, MA) in 70 mM Tris/HCl pH7.6 ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1 μL of 10 mM dNTP mix (NEB N0447S, Ipswich, MA). This initial premix was heated at 65°C for 5 minutes ...
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bioRxiv - Genomics 2023Quote: ... 1 μl 10 mM mix of dGTP and dTTP (NEB #N0442S, #N0443S), 5 μl 10x T4 DNA Ligase Buffer ...
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bioRxiv - Genomics 2023Quote: ... 12 μL of 10 mg/mL Bovine Serum Albumin (100 × BSA, NEB), 5 μL of 400 U/μL T4 DNA Ligase (NEB #M0202) ...
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bioRxiv - Biophysics 2023Quote: ... and dGTP were incubated and 10 units of DNA polymerase 1 (NEB) for 6 minutes at 37°C ...
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bioRxiv - Systems Biology 2023Quote: ... 10 μl of NEB T4 ligation buffer (New England Biolabs, Ipswitch, MA), 5 μl of NEB T4 ligase (New England Biolabs ...
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Bio-orthogonal Glycan Imaging of Culture Cells and Whole Animal C. elegans with Expansion MicroscopybioRxiv - Bioengineering 2024Quote: ... and treated with 10 mM DTT and Proteinase K (New England Biolabs) at 8 units/mL in digestion buffer (50 mM Tris (pH 8) ...
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bioRxiv - Cell Biology 2023Quote: ... freshly supplemented with 10 U/ ml proteinase K (P8107S, New England Biolabs). Plates were slightly tilted to ensure the coverslip was completely covered and incubated at room temperature overnight ...
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bioRxiv - Genomics 2024Quote: ... and the top 3 sgRNAs were individually cloned into single sgRNA expression vectors with direct capture cs1 sequences (Addgene # 122238)10 using restriction digest (BstXI and BlpI) and T4 ligation (NEB # M0202M). A non-targeting sgRNA sequence was included as well ...
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bioRxiv - Genomics 2024Quote: ... 10 µg of RNA was treated with 1 µL Xrn1 (NEB, M0338S) for 1 h at 37 °C to remove decapped ...
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bioRxiv - Biochemistry 2024Quote: ... 2.5 μL 10 mM MnCl2 and 2 μL Lambda Protein Phosphatase (NEB). The dephosphorylation reaction was allowed to occur at 30 °C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... A preheated mixture of 10 µl of RNase H (New England Biolabs) and 2 µl of RNase H Buffer (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... 10 μl of proteinase K at 20mg/ml (NEB cat no. P8107S) and a 2% final concentration of SDS was added and the mixture incubated for 1 h at 37 °C ...
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bioRxiv - Plant Biology 2020Quote: ... APE1 gene with an additional 1500 bp on the 5’ side and 500 bp on the 3’ side was amplified by PCR using Q5 High-Fidelity DNA Polymerase (NEB) using primers 5’-TTATAATACCCACCCGTCAAAGCTGTG-3’ and 5’-TTATAACAGACTCATCCGGACCCCAA-3’ containing an additional PsiI restriction site (70°C ...
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bioRxiv - Plant Biology 2019Quote: ... For alkaline phosphatase treatment, microsomal pellets were obtained as described previously (Inada et al., 2004) and resuspended in the 1× NEBuffer 3 (NEB) with 0.5% Triton-X100 and protease inhibitor mixture (Complete Mini EDTA-free ...
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bioRxiv - Biochemistry 2020Quote: ... to 3 μM concentration in a total volume of 50 μl and mixed with 20 μl amylose beads (New England BioLabs). After mixing the proteins and the beads ...
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bioRxiv - Biochemistry 2021Quote: ... A 5′-adenylated R1R adapter was then ligated to the 3′ end of the cDNA using Thermostable 5′ App DNA/RNA Ligase (New England Biolabs) for 1 h at 65 °C ...