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Citations for New England Biolabs :
2501 - 2550 of 5199 citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 1 μL of 10 mM dNTPs (NEB N0447L), 1 μL GC2 polymerase ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 μl T4 DNA ligase buffer (ThermoFisher/NEB), 0.5 μl AarI (ThermoFisher ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 µL of CutSmart Buffer (NEB #B6004S) for one hour at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 μL Klenow polymerase (NEB cat#M0210S) was added to the annealed oligos ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 μL dNTP’s (10mM each, NEB cat#FERR0192) and 1 μL Klenow polymerase (NEB cat#M0210S ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We added 1 µL of ApaLI (NEB #R0507L) and 5.5 µL of Cutsmart Buffer (NEB #R0507L ...
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bioRxiv - Genomics 2024Quote: ... 1 μL T4 RNA ligase reaction buffer (NEB), 1 μL RNAseOUT (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 1 U/µL rNase inhibitor (NEB) and immediately frozen on dry ice.
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bioRxiv - Microbiology 2024Quote: ... supplemented with 1 U/µL rNase inhibitor (NEB) and immediately frozen on dry ice.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 1 µL CspCI (5,000 units/mL; NEB) in a 50 µL reaction ...
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bioRxiv - Microbiology 2024Quote: ... 1 U/µL Murine RNAse Inhibitor (NEB #M0314), 62.5 nM 6U_FAM_reporter (Table S1) ...
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bioRxiv - Microbiology 2024Quote: ... and 1 µl Q5 DNA polymerase (NEB M0491). Thermocycling conditions were ...
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bioRxiv - Neuroscience 2024Quote: ... or CLIP-Surface 647 (1:1000; NEB; S9234S) in 0.3% PBT for 15 min and washed with 3×20 min with 0.1% PBT ...
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bioRxiv - Neuroscience 2024Quote: ... 1×T4 ligase buffer with ATP (NEB, B0202), 5% (w/v ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 μl blunting enzyme mix (NEB; # M1201AVIAL)] ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 µL Purple Dye (New England Biolabs, B7025S), and 4 uL ddH20 ...
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bioRxiv - Plant Biology 2024Quote: ... 1 U Q5 DNA polymerase (New England Biolabs), 2 µL of the first cDNA strand for CYP7 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 μl BSA (20 mg/ml; NEB; #B9200S) and 81 μl H2O ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 μl dATP solution 100 mM (NEB; # N0440S), 1 μl BSA (20 mg/ml ...
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bioRxiv - Molecular Biology 2024Quote: ... mouse antibodies against MBP (NEB E8032L, 1:20,000) were used to detect 2XMBP-tagged proteins and rabbit antibodies against HA (Cell Signaling C29F4 ...
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bioRxiv - Genomics 2024Quote: ... a ligation mix containing 1× T4 buffer (NEB) and 20 U/μl T4 DNA ligase (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... 1 μM RNCs or empty 70S ribosomes (NEB) were incubated (30 min ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 1 x RNAPol reaction buffer (NEB, B9012S). After transcription ...
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bioRxiv - Cell Biology 2024Quote: ... 1 U/µl T4 PNK enzyme (NEB #M0201S) and 5 mM DTT (freshly added) ...
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bioRxiv - Plant Biology 2024Quote: ... and 1 µL of CutSmart Buffer (NEB #B6004S) and incubated for one hour at 37°C prior to a 20- minute heat inactivation at 80°C ...
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bioRxiv - Genomics 2024Quote: ... 1% v/v proteinase K (New England Biolabs), 50 mM Tris pH 8 (Ambion) ...
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bioRxiv - Biochemistry 2024Quote: ... and 1 U/µl Murine RNAse inhibitor (NEB)) ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mL PNGase F (P0708L, New England Biolabs), and H2O as necessary to a final volume of 20 mL ...
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bioRxiv - Bioengineering 2024Quote: ... 1 μL dATP at 10 mM (NEB, N0440S), and 1 μL Taq polymerase (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... anti-MBP (mouse, 1:30,000, New England Biolabs), anti-FLAG (mouse ...
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bioRxiv - Developmental Biology 2024Quote: ... and 1 μL USER Enzyme (NEB, Cat. M5505) to nick ...
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bioRxiv - Genetics 2024Quote: ... and 1 µL Taq DNA Polymerase (NEB, M0273) were added ...
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bioRxiv - Microbiology 2020Quote: ... 3 μL of in vitro transcribed 300 nM sgRNA or trL (produced as described in in vitro transcription protocol above) and 1 μL of 1 μM Cas9 nuclease (NEB, M0386S) were mixed and incubated for 10 minutes at 25°C to allow for RNP formation ...
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bioRxiv - Cell Biology 2021Quote: ... 1% NP-40, 1 mM DTT, 0.1 mg/ml cycloheximide, 1.25 mg/ml heparin, 400 U/ml NEB murine RNase inhibitor) and dounced ...
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bioRxiv - Genomics 2022Quote: ... Illumina libraries and DNA splint were mixed at a 1:1 ng ratio using NEBuilder HiFi DNA assembly Master mix (NEB #E2621). Any non-circularized DNA was digested overnight using ExoI ...
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bioRxiv - Microbiology 2020Quote: ... The flies were homogenised in 100 μl of TE-buffer pH 8 containing 1% Triton X-100 and 1% Proteinase K (NEB, P8107S). Homogenates were incubated for 3 h at 55°C followed by a 10 min incubation step at 95°C ...
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bioRxiv - Immunology 2022Quote: ... cleavage rates were transformed into standardized catalytic activities (1 “eqU” = equivalent to the catalytic activity of 1 U RNase HII (NEB ®) under standard conditions) ...
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bioRxiv - Molecular Biology 2020Quote: ... was linked to the free hydroxyl group at the 3’-end of transcripts (1 □g of total RNA) by T4 RNA ligase 1 (NEB M0204) in the presence of 15% (w/v ...
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bioRxiv - Molecular Biology 2024Quote: For proximity ligation, the pellet was resuspended into 500 μL of ligation mix (1× T4 DNA ligase buffer, 1× BSA (NEB, B9000S), 5000 CEU T4 DNA ligase (NEB ...
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bioRxiv - Immunology 2024Quote: ... Insert and vector were combined at a 5:1 ratio and held at 50 °C for 1 hour in NEBuilder® HiFi DNA Assembly Master Mix (NEB). Gibson assembly products were purified using the MinElute® PCR Purification Kit (Qiagen) ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... top and bottom oligonucleotides for a single R-S-R or dead end oligonucleotide pairs were combined 1 µL:1 µL and were phosphorylated in a 50 µL reaction using T4 Polynucleotide Kinase (NEB M0201L) for 2 h at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... cells were live labeled with 1 μM ATTO 590-chloroalkane and 1 μM of SNAP-Cell 647-SiR (New England Biolabs; S9102S) (final concentrations) ...
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bioRxiv - Genomics 2023Quote: ... cells were finally resuspended in 400ul PSB-DTT at the concentration of ∼2e6 cells/100ul (PBS, 1% SUPERase In, 1% BSA (NEB B90000S), 1mM DTT) ...
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bioRxiv - Genomics 2024Quote: ... of 0.1 μM polyT-rootV2 oligo diluted in hybridization buffer (2x SSC, 1 mg/mL yeast RNA, 10% dextran sulfate, 1:1000 Murine RNase inhibitor NEB M0314) on a parafilm sheet placed on a glass plate ...
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bioRxiv - Molecular Biology 2024Quote: ... ssSynth primer was extended by adding 1 μL 10mM dNTP’s (Fisher cat#FERR0192) and 1 μL Klenow polymerase (NEB cat#M0210S) then incubating at 37°C for 1hr ...
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bioRxiv - Neuroscience 2024Quote: ... and end repair was performed by incubating chromatin-bound beads for 1 h at room temperature in 1× T4 ligase buffer with ATP (NEB, B0202), 0.25 mM dNTPs (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2024Quote: ... The genes were assembled into the expression vector pMC-1-1-1 cut with NotI (Clark et al., 2022) through HiFi DNA assembly (NEBuilder HiFi DNA Assembly Master Mix NEB#E2621) into E ...
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bioRxiv - Bioengineering 2024Quote: ... To generate the proper overhangs for insertion into the Effector 1 position, clonal effectors were predigested with BsaI (600 ng clonal fragment, 1 μL BsaI-HFv2 (NEB, R3733L), 1 μL Quick CIP (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 µL of the PCR product was incubated with 2 U of EcoRI-HF® in 1X CutSmart® Buffer (New England BioLabs, Ipswich, MA, USA) at 37°C for 60 minutes ...
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bioRxiv - Neuroscience 2021Quote: A total amount of 0.4 ug of RNA was used for cDNA library construction at Novogene using an NEBNext® Ultra 2 RNA Library Prep Kit for Illumina® (cat NEB #E7775, New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...