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Citations for New England Biolabs :
201 - 250 of 2549 citations for Human PDGF alpha receptor PDGFRA qPCR Primer Pair since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... 1 μg of total RNA was depleted of rRNA using NEBNext rRNA Depletion Kit (Human/Mouse/Rat) prior to cDNA synthesis primed with random hexanucleotide oligonucleotides (Random Primer 6, NEB). Sequencing library construction was carried out using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB) ...
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bioRxiv - Neuroscience 2024Quote: Enhancers were cloned from human or C57Bl/6J mouse genomic DNA using enhancer-specific primers and Phusion high-fidelity polymerase (M0530S; NEB). Individual enhancers were then inserted into a recombinant single-stranded pAAV backbone that contained the beta-globin minimal promoter ...
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bioRxiv - Molecular Biology 2024Quote: ... mRNA isolated from Human Bronchial Epithelial Cells was reverse transcribed using oligo dT primers and ProtoScript II (New England Biolabs) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Universal PCR primers and Index (X) Primer (New England BioLabs). At last ...
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bioRxiv - Plant Biology 2020Quote: ... The enzyme pair HinfI and HpyCH4IV (New England Biolabs, Ipswich, USA) was selected based on the number of fragments ...
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bioRxiv - Molecular Biology 2022Quote: qPCR was performed using the Luna qPCR mix (NEB) to measure the relative amount of origin DNA compared to the terminus ...
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bioRxiv - Molecular Biology 2022Quote: qPCR was performed using the Luna qPCR mix (NEB) to measure the amount of genomic loci bound to DnaA ...
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bioRxiv - Molecular Biology 2022Quote: qPCR was performed using the Luna qPCR mix (NEB) to measure the relative amount of origin DNA compared to the terminus ...
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bioRxiv - Molecular Biology 2022Quote: qPCR was performed using the Luna qPCR mix (NEB) to measure the amount of genomic loci bound to DnaA ...
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bioRxiv - Microbiology 2024Quote: qPCR was performed using the Luna qPCR mix (NEB) to measure the relative amount of origin DNA compared to the terminus ...
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bioRxiv - Cell Biology 2021Quote: ... ORF was amplified from MCF10A human mammary cells using specific primers (listed in Table S2) with Q5 High-Fidelity 2X Master Mix (New England BioLabs, # M0492), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... and Illumina linker addition to B cell heavy chain transcripts were performed using the human heavy chain only primers of NEBNext Immune Sequencing Kit (New England Biolabs #E6320) according to the manufacturer’s instructions (provided upon request) ...
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bioRxiv - Genomics 2022Quote: Library E was transformed into 5-alpha E.coli (NEB, C2987H), recovered in SOC medium (NEB ...
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bioRxiv - Systems Biology 2021Quote: ... The libraries were transformed into 5-alpha electrocompetent cells (NEB) and grown in liquid culture ...
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bioRxiv - Systems Biology 2021Quote: ... The libraries were transformed into 5-alpha electrocompetent cells (NEB) and grown in liquid culture.
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bioRxiv - Biophysics 2022Quote: ... Toxicity-resistant strains such as NEB 5-alpha F’Iq (NEB) and ABLE K (Agilent ...
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bioRxiv - Microbiology 2022Quote: ... coli dam+/dcm+ (NEB 5-alpha competent E. coli, #C2987) and dam−/ dcm− (dam−/dcm− competent E ...
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bioRxiv - Biochemistry 2022Quote: ... T4 DNA ligase and chemically competent DH5 alpha cells (NEB); Alt-R CRISPR-Cas9 crRNA ...
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bioRxiv - Microbiology 2021Quote: ... coli DH5-alpha cells following manufacturer’s protocol (New England Biolabs). Sanger sequencing was used to confirm plasmid construction ...
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bioRxiv - Molecular Biology 2021Quote: ... and NEB® 5-alpha F’IQ (New England Biolabs, NEB) were used for routine cloning procedures and chemically competent E ...
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bioRxiv - Molecular Biology 2021Quote: ... and NEB® 5-alpha F’IQ (New England Biolabs, NEB) were used for routine cloning procedures and chemically competent E ...
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bioRxiv - Synthetic Biology 2022Quote: ... Assembled products were heat-shock transformed to 5-alpha (NEB), TOP10 (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... Assembled plasmids were transformed into NEB 5-alpha F’Iq (NEB) and standard chemically competent Escherichia coli transformation protocols were used to construct plasmid host strains ...
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bioRxiv - Bioengineering 2024Quote: ... grown in 5-alpha competent Escherichia coli (NEB, Ipswich, MA) and purified using the PureYield MidiPrep System (Promega ...
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bioRxiv - Genomics 2023Quote: ... The library was transformed into 5-alpha electrocompetent cells (NEB), grown in liquid culture ...
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bioRxiv - Genomics 2023Quote: ... The libraries were transformed into 5-alpha electrocompetent cells (NEB). The plasmid library was prepared by maxi-prep (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... NEB 5-alpha competent E.coli (NEB #C2987H, Ipswich, MA, USA) were transformed with ligated plasmid by heat shock at 42°C for 30 seconds ...
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bioRxiv - Synthetic Biology 2024Quote: ... Commercially available NEB5- alpha chemically competent cells (NEB, Ipswich, MA) were used for routine transformations ...
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bioRxiv - Microbiology 2024Quote: Chemically competent NEB® 5-alpha F’Iq cells (NEB; C2992H) were transformed with recombinant plasmids per manufacturer instructions and grown at 37°C on selective Luria-Bertani (LB ...
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bioRxiv - Bioengineering 2020Quote: ... each forward/reverse oligo pair was annealed in T4 Ligation Buffer (NEB), with T4 PNK to phosphorylate the oligos ...
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bioRxiv - Genomics 2020Quote: Each oligos pair was further phosphorylated and annealed using T4 PNK (NEB) and T4 Ligation Buffer (NEB) ...
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bioRxiv - Cancer Biology 2023Quote: ... Annealed sgRNA oligo pairs were phosphorylated with T4 PNK (NEB, USA #M0201S) and ligated with Quick Ligase (NEB ...
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bioRxiv - Cancer Biology 2023Quote: ... specific i7 and i5 NEBNext index pairs (5uM) (New England Biolabs, U.S) were added to each sample and incubated as follows ...
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bioRxiv - Plant Biology 2021Quote: ... qPCR was performed using Kapa SYBR FAST qPCR Master Mix or Luna Universal qPCR Master Mix (NEB) on a QuantStudio 3 system ...
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bioRxiv - Microbiology 2023Quote: ... qPCR was performed in 20 µL reactions using Luna qPCR Universal qPCR Master Mix (New England Biolabs), 5 ng of cDNA as template ...
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bioRxiv - Cell Biology 2021Quote: ... and DNA fragments were amplified by PCR with NEXTFlex primers (Primer 1 - 5’-AATGATACGGCGACCACCGAGATCTACAC; Primer 2 - 5’-CAAGCAGAAGACGGCATACGAGAT) and Phusion High-Fidelity DNA Polymerase (NEB) and further purified with AMPure XP beads to eliminate unligated primers and adapters ...
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bioRxiv - Cell Biology 2021Quote: ... Libraries were amplified by PCR using NextFlex PCR primers (Primer 1 - 5’-AATGATACGGCGACCACCGAGATCTACAC; Primer 2 - 5’-CAAGCAGAAGACGGCATACGAGAT) and Phusion High-Fidelity DNA Polymerase (NEB) before three further rounds of AMPure XP purification were performed to collect fragments 150-300 bp in size ...
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bioRxiv - Genetics 2022Quote: ... pipientis infection status by qPCR (NEB Luna Universal qPCR kit) using primer sets for wsp and arm (S1 Table) ...
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bioRxiv - Immunology 2022Quote: ... RT-qPCR was performed with Luna Universal qPCR reagent (NEB). eMLV env was detected with 5’-AGGCTGTTCCAGAGATTGTG-3’ and 5’-TTCTGGACCACCACATGAC-3’ and 18S rRNA was detected with 5’-GTAACCCGTTGAACCCCATT-3’ and 5’-CCATCCAATCGGTAGTAGCG-3’ on Roche LightCycler 480 II (104,105).
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bioRxiv - Plant Biology 2024Quote: ... qPCRs were done using qPCR Master Mix (NEB, M3003, www.neb.com) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... qPCR was performed using Luna Universal qPCR Master Mix (NEB) with Bio-Rad iQ5 multicolor real-time PCR detection system ...
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bioRxiv - Immunology 2023Quote: ... qPCR was performed using Luna universal QPCR mix (NEB # M3003) in the CFX96 Real-Time PCR system with a C1000 Thermal Cycler (Bio-Rad) ...
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bioRxiv - Systems Biology 2024Quote: ... qPCR was performed using Luna qPCR master mix (NEB, M3004).
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bioRxiv - Evolutionary Biology 2024Quote: ... qPCR was conducted using the Luna qPCR Master Mix (NEB), with two technical replicates per cDNA sample ...
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bioRxiv - Genetics 2024Quote: ... qPCR was performed with LUNA qPCR MasterMix (NEB Cat# M3003) on a QuantStudio 3 Real-Time PCR System (Fisher Cat# A28566 ...
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bioRxiv - Microbiology 2023Quote: ... Primers were designed with the NEB primer design tool (New England Biolabs).
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bioRxiv - Genomics 2023Quote: ... Universal PCR Primer for Illumina and index primer for Illumina (NEB E7335S), and 2X Kapa HiFi HotStart ReadyMix (Kapa KK2611) ...
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bioRxiv - Genomics 2023Quote: ... Universal PCR Primer for Illumina and index primer for Illumina (NEB E7335S), and 2X Kapa HiFi HotStart ReadyMix (Kapa KK2611) ...
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bioRxiv - Molecular Biology 2020Quote: ... random primer (NEB #S1330S) (3uM) ...
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bioRxiv - Molecular Biology 2022Quote: ... appropriate index primer (NEB Single Index ...