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Citations for New England Biolabs :
1 - 50 of 2549 citations for Human PDGF alpha receptor PDGFRA qPCR Primer Pair since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Unique dual index primer pairs (New England Biolabs) were used to barcode single-cell libraries ...
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bioRxiv - Cancer Biology 2024Quote: ... Unique dual index primer pairs (New England Biolabs) were used to barcode single -cell libraries ...
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bioRxiv - Molecular Biology 2022Quote: ... A different Unique Dual Index Primer Pair (NEB, E6440S) was added to each sample and 11 cycles were used for the PCR enrichment ...
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bioRxiv - Biochemistry 2023Quote: ... was PCR amplified with mutagenic primer pairs using Q5 polymerase (NEB) (Supplementary Table S1) ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR conditions were followed according to each primer pair as recommended by NEB using pCMV-SMAUG1-HisTEV2xFLAG as the template ...
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bioRxiv - Synthetic Biology 2022Quote: ... and 1 µl of a dual unique index multiplex primer pair (NEB; E6440) in a total reaction volume of 25 µl ...
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bioRxiv - Genetics 2023Quote: ... and NEBNext Multiplex Oligos for Illumina (96 Unique index primer pairs) (E6440, NEB). We used 13 min fragmentation time and 9 cycles of PCR enrichment on 1000 ng of total RNA input determined by preliminary library preparation ...
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bioRxiv - Genetics 2024Quote: ... and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (NEB). Sequencing was performed using a SP Reagent Kit (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... These primer pairs were assembled by heat-denaturation and annealing in Buffer 3.1 (NEB), then cloned into BbsI-linearized pX458 (62 ...
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bioRxiv - Neuroscience 2024Quote: ... and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (NEB E6440S). Library cleanup was performed prior to and after PCR amplification using 0.8X Kapa Hyperpure beads (Roche 08963851001) ...
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bioRxiv - Immunology 2023Quote: ... PCR was then performed on genomic DNA with relevant primer pair and enzyme (NEB) (see materials and methods chapter for PCR programme) ...
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bioRxiv - Molecular Biology 2023Quote: ... and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (NEB #E6440S). Library cleanup was performed prior to and after PCR amplification using 0.8X Kapa Hyperpure beads (Roche #08963851001) ...
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bioRxiv - Molecular Biology 2024Quote: ... libraries were indexed and amplified with NEBNext UDI primer pairs (New England Biolabs, #E6440S) during 8 PCR cycles with NEBNext HiFi PCR Master mix (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... and NEBNext Multiplex Oligos for Illumina Unique Dual Index Primer Pairs (NEB #6442S/L). Sequencing of the libraries was performed with Illumina NovaSeq 6000 SP flow cell using paired-end 150-bp format (300 cycles = 2×150bp ...
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bioRxiv - Immunology 2021Quote: ... Pairs of functional Ig genes were PCR-amplified using specific primers with Q5 Polymerase (NEB). PCR-product and linearized vector containing the constant part of IgG1 heavy or kappa/lambda light chain sequences respectively were assembled using Gibson cloning with HiFi DNA Assembly Master Mix (NEB) ...
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bioRxiv - Immunology 2020Quote: ... Pairs of functional Ig genes were PCR-amplified using specific primers with Q5 Polymerase (NEB). PCR-product and linearized vector were assembled using Gibson cloning with HiFi DNA Assembly Master Mix (NEB) ...
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bioRxiv - Biophysics 2023Quote: ... each primer pair was annealed and consequently phosphorylated with T4-polynucleotide kinase (T4-PNK; NEB). Restriction digestion of the pLentiCRISPRv2 vector was performed using FD-Esp3I (Thermo ...
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bioRxiv - Microbiology 2024Quote: ... and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs; New England BioLabs) were used for creating a genomic DNA library ...
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bioRxiv - Microbiology 2024Quote: ... and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (New England Biolabs), following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and the NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (NEB, E6440S) and purified using the SPRIselect bead-based reagent (Beckman Coulter ...
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bioRxiv - Cancer Biology 2020Quote: ... Illumina compatible NEBNext Multiplex Dual Index Primer pairs and Adaptors (New England Biolabs, Cat. No. E6440S) were then ligated to the ends of the size-selected chimeric fragments ...
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bioRxiv - Cancer Biology 2023Quote: ... Illumina-compatible NEBNext Multiplex Dual Index primer pairs and adapters (New England Biolabs, cat. no. E6440S) were added to each sample ...
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bioRxiv - Cancer Biology 2023Quote: ... and NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs, NEB, E6440) as per manufacturer’s recommendation ...
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bioRxiv - Genetics 2023Quote: ... and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs Set 4) (NEB, E6446S) as index primers ...
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bioRxiv - Evolutionary Biology 2023Quote: ... with NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (New England Biolabs E6440S) and polyA enrichment module (NEB E7490S) ...
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bioRxiv - Cell Biology 2023Quote: ... 45-72°C for 30 seconds (depending on melting temperature of primer pairs determined by NEB Tm calculator https://tmcalculator.neb.com) ...
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bioRxiv - Developmental Biology 2023Quote: ... in conjunction with NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (NEB #E6440S), according to the manufacturer’s instructions ...
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A humoral immune response to parasitoid wasps in Drosophila is regulated by JAK/STAT, NF-κB and GATAbioRxiv - Immunology 2024Quote: ... with NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (New England Biolabs #E64460S) and polyA enrichment module (NEB #E7490S) ...
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bioRxiv - Synthetic Biology 2024Quote: ... The Level 0 plasmid was amplified with the primer pair SLo0765/SLo0766 followed by DpnI (NEB) digestion prior to ligation (any Level 0 plasmid will do ...
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bioRxiv - Immunology 2021Quote: ... Libraries were indexed using NEBNext Multiplex Oligos for Illumina Unique Dual Index Primer Pairs (NEB #644OS/L). Library prep generated cDNA was quantified and analyzed using Agilent DNA chip and Qubit DNA dye ...
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bioRxiv - Microbiology 2023Quote: ... Primer pairs M01/M02 and M03/M04 were used with the Q5 site-directed mutagenesis kit (NEB) to introduce point mutations for the T161V and D296N substitutions ...
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bioRxiv - Microbiology 2024Quote: Suitable primer pairs (Table S5) were designed utilizing the Q5 site-directed mutagenesis kit (New England Biolabs) to generate mutated amidases ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and the NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs, NEB #E6440). Quality control of the libraries was done with the Qubit dsDNA HS Assay Kit (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... using NEBNext Multiplex Oligos for Illumina – 96 Unique Dual Index Primer Pairs (#6440S, New England Biolabs, US). All generated cDNA libraries were amplified with 7 cycles of final PCR.
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bioRxiv - Developmental Biology 2024Quote: ... 8 PCR cycles for enrichment of adaptor-ligated DNA with unique dual index primer pairs from NEB. The libraries were sequenced on the NovaSeq 6000 system with NovogeneAIT Genomics ...
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bioRxiv - Microbiology 2023Quote: ... in combination with NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs; New England BioLabs), following the manufacturers’ recommended protocols ...
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bioRxiv - Neuroscience 2023Quote: ... Libraries were indexed using NEBNext Multiplex Oligos for Illumina Unique Dual Index Primer Pairs (NEB #644OS/L). Library prep generated cDNA was quantified and analyzed using Agilent DNA chip and Qubit DNA dye ...
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bioRxiv - Genomics 2023Quote: ... with NEBNext Multiplex Oligos (96 Unique Dual Index Primer Pairs) for Illumina Barcodes (New England Biolabs, USA) and sequenced on an Illumina NovaSeq 6000 S2 150bp PE flow cell at the Duke Center for Genomic and Computational Biology Sequencing and Genomic Technologies Core.
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Fluoroquinolone-Specific Resistance Trajectories in E. coli and their Dependence on the SOS-ResponsebioRxiv - Microbiology 2024Quote: ... in combination with NEBNext multiplex oligos for Illumina (96 Unique Dual Index Primer Pairs; New England BioLabs). The samples were sequenced using a NextSeq 500/550 Mid Output V2.5 kit (Illumina).
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bioRxiv - Microbiology 2024Quote: ... in combination with NEBNext multiplex oligos for Illumina (96 Unique Dual Index Primer Pairs; New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... and the NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (NEB, Cat. No. E6440S) and purified using the SPRIselect bead-based reagent (Beckman Coulter ...
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bioRxiv - Neuroscience 2024Quote: ... Adaptor-ligated DNA fragments were amplified using NEBNext Unique Dual Index Primer Pairs (New England BioLabs, E6441A) with NEBNext Ultra II Q5 Master Mix (M0544S ...
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bioRxiv - Microbiology 2024Quote: ... using a specific primer pair (Supplementary table 1) and Q5 High-Fidelity DNA Polymerase (New England Biolabs). The PCR product was purified using a MinElute Reaction Cleanup kit (Qiagen) ...
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bioRxiv - Developmental Biology 2024Quote: ... and the NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (NEB, Cat. No. E6440S) and purified using the SPRIselect bead-based reagent (Beckman Coulter ...
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bioRxiv - Neuroscience 2024Quote: ... Adaptor-ligated DNA fragments were amplified using NEBNext Unique Dual Index Primer Pairs (E6441A, New England BioLabs) with NEBNext Ultra II Q5 Master Mix (M0544S ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the libraries were indexed using NEBNext Multiplex Oligos for Illumina 96 Unique Dual Index Primer Pairs (NEB). The prepared libraries were quantified via a fluorometric method involving Qubit High Sensitivity (Invitrogen ...
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bioRxiv - Genetics 2024Quote: ... and the NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (NEB, Cat. No. E6440S) and purified using the SPRIselect bead-based reagent (Beckman Coulter ...
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bioRxiv - Synthetic Biology 2021Quote: ... qPCR was carried out using the primers in Supplementary Table 3 and Luna Universal qPCR Master Mix (NEB) in a BioRad iCycler in technical triplicates for each biological replicate ...
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bioRxiv - Cell Biology 2022Quote: ... we used three pairs of specific primers (Supplementary Table S2) with Q5 High Fidelity DNA Polymerase (NEB, M0491) or Phusion High Fidelity DNA Polymerase (Thermo Scientific Inc F530S) ...
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bioRxiv - Synthetic Biology 2024Quote: ... The level 0 acceptor plasmid was amplified with the primer pair SLo0765/SLo0766 using Q5 DNA Polymerase (NEB) followed by a DpnI (NEB ...