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Citations for New England Biolabs :
2401 - 2450 of 7435 citations for rno mir 206 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... PCR product using T7 RNA polymerase (NEB, M0251L) as described previously 18 ...
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bioRxiv - Biochemistry 2024Quote: ... PCRs were performed with 2xQ5 HotStart MasterMix (NEB). Plasmids were generated by Q5 Site-Directed Mutagenesis or Gibson Assembly (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... Colony PCR with OneTaq 2X master mix (NEB) were used to verify loss of target gene and correct cassette integration86 ...
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bioRxiv - Neuroscience 2024Quote: ... and purified with Monarch PCR cleanup kit (NEB). Purified amplicons were sequenced by Plasmidsaurus ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR reactions were performed using NEBNext Polymerase (NEB) using the primer sets listed in Supplementary Table 7 ...
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bioRxiv - Systems Biology 2024Quote: ... 2 µL of 10X Thermo PCR buffer (NEB), 2 µL of ES-E14 wildtype cDNA template or from 1 µL plasmid pLJM1-EGFP for GFP esiRNAs ...
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bioRxiv - Synthetic Biology 2024Quote: ... using Phusion© High-Fidelity PCR Kit (NEB) with annealing temperature at 62 ◦C ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR fragments were amplified using Q5 polymerase (NEB). Vectors were digested with NEB restriction enzymes and ligated to gel-purified PCR products using T4 ligation ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and performed colony PCRs with Taq polymerase (NEB). We used the Monarch PCR and DNA Cleanup Kit (NEB ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products were treated with exonuclease (NEB) and shrimp alkaline phosphatase (TaKaRa) ...
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bioRxiv - Molecular Biology 2024Quote: ... qRT-PCR was performed using LUNA SYBR (NEB) on a Rotorgene (Qiagen) ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 RNA levels were quantified using the Luna Universal Probe Onestep RT-qPCR kit (New England Biolabs) and US CDC real-time RT-PCR primer/probe sets for 2019-nCoV_N1 ...
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bioRxiv - Microbiology 2022Quote: ... Five microliters of the mixture was added to Luna Universal Probe One-Step RT- qPCR mixture (NEB, MA, USA) to a final volume of 20 µl ...
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bioRxiv - Genomics 2020Quote: ... RT adapters (supplied in the kit) were ligated to the RNA strand using T4 DNA ligase (New England Biolabs). Purification steps were performed using Agencourt AMPure XP magnetic beads (Beckman Coulter ...
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bioRxiv - Molecular Biology 2019Quote: ... SI) were incubated for 2 hours at RT in T4 ligase buffer with 400 U of T4 ligase (NEB) in a total volume of 100 µl ...
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bioRxiv - Cell Biology 2021Quote: ... 1 µl was added to a 9 µl Luna® Universal One-Step RT-qPCR reaction (NEB, Cat# E3005L). RT-qPCR was carried out to manufacturer specifications in a CFX96 Touch Real Time PCR Machine (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... and OC43) viral RNA genomes were synthesized using the LunaScript RT SuperMix Kit (New England Biolabs, Ipswich, MA, USA). The reaction mixture had a volume of 20◻μL and comprised of 4◻μL of 5X LunaScript RT supermix ...
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bioRxiv - Microbiology 2021Quote: ... using the IP4 set of primers and probe as described on the WHO website (https://www.who.int/docs/default-34source/coronaviruse/real-time-rt-pcr-assays-for-the-detection-of-sars-cov-2-institut-35pasteur-paris.pdf?sfvrsn=3662fcb6_2) and the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs, France).
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bioRxiv - Microbiology 2020Quote: ... SCV2 RNA was quantified using a NEB Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs, E3006) and 2019-nCoV CDC N1 primers and probes (IDT ...
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bioRxiv - Microbiology 2022Quote: ... Reverse transcription of 10 µl RNA was performed using LunaScript RT Supermix Kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... modified and CE treated IVT RNA were ligated to ONT RT Adapter using concentrated T4 DNA ligase (NEB-M0202T), and was reverse transcribed using SuperScript III Reverse Transcriptase (Thermo Fisher Scientific-18080044) ...
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bioRxiv - Microbiology 2020Quote: ... THUNDERBIRD Probe qPCR Master Mix (Toyobo) for MERS-CoV and Luna Universal Probe One-Step RT-qPCR Kit (NEB) for SARS-CoV-2.
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bioRxiv - Microbiology 2020Quote: ... RdRP_SARSr-F2 (GTGARATGGTCATGTGTGGCGG) and dRP_SARSr-R1(CARATGTTAAASACACTATTAGCATA) using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) and a CFX96 Real-Time System ...
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bioRxiv - Microbiology 2021Quote: ... Or reverse transcription and qRT-PCR were conducted in one step using 0.1 μg RNA as template with the Luna Universal Probe One-Step RT-qPCR kit (New England Biolabs) following manufacturer’s protocols ...
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bioRxiv - Immunology 2022Quote: ... 5µl of RNA (roughly corresponding to 1µg) was reverse transcribed to cDNA using the LunaScript RT SuperMix kit - dye based qPCR detection (NEB) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The RT reaction was then amplified using PHUSION® high-fidelity DNA polymerase using HF buffer (New England Biolabs) via PCR to generate a 1675-bp amplicon ...
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bioRxiv - Plant Biology 2022Quote: ... 1 μg of total RNA was used for cDNA synthesis using the LunaScript RT SuperMix Kit (New England Biolabs) according to the manufacturer’s instruction ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Quantification of viral genomes was performed using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) with a primer/probeset binding in the orf1ab region (forward ...
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bioRxiv - Systems Biology 2024Quote: ... 5µl of RNA (roughly corresponding to 1µg) was reverse transcribed to cDNA using the LunaScript RT SuperMix kit - dye based qPCR detection (NEB) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... P13-P19) (73) were carried out using Luna Universal One-Step RT-qPCR Kit (New England Biolabs; Frankfurt (Main), Germany ...
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bioRxiv - Developmental Biology 2023Quote: ... equivalent quantities of total RNA were used for cDNA synthesis with the LunaScript RT SuperMix Kit (New England Biolabs). For RT-PCR ...
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bioRxiv - Microbiology 2023Quote: ... Two step qPCR was carried out firstly by converting RNA into cDNA following the manufacturer’s instruction for LunaScript RT SuperMix Kit (New England BioLabs). Subsequently ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was eluted from the gel overnight in 400 µl of 0.4 M NaCl with rotation at RT and precipitated with 96% ethanol in the presence of linear acrylamide (NEB).
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bioRxiv - Molecular Biology 2023Quote: ... samples were washed in CutSmart buffer, and incubated (1h, RT) in 150 µL blunting mix (112.5 µL ddH2O, 15 µL 10x blunting buffer, NEB, 15 µL 100 µM dNTPs ...
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bioRxiv - Pathology 2023Quote: ... Each 10 µL reaction contained Luna Probe One-Step RT-qPCR 4X Mix (New England Biolabs, Ipswich, MA, USA), primers (10 µM) ...
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bioRxiv - Microbiology 2023Quote: ... Glycans were removed by incubating the immunoadhesins ON at room temperature (RT) with endoglycosidase H (endo-H, 0.8 U/mL, #P0702L, New England Biolabs) in 50 mM Na acetate-50 mM NaCl ...
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bioRxiv - Physiology 2023Quote: ... cDNA was generated from 500ng of RNA using the LunaScript RT Supermix kit (New England Biolabs, Cat. No. E3010L) according to the manufacturer’s directions ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was performed using 10-fold-diluted cDNA and the Luna Universal qPCR Master Mix (New England Biolabs) in the CFX Real-Time PCR system (Bio-Rad) ...
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bioRxiv - Genomics 2024Quote: ... cDNA was synthesized from each sample using LunaScript® RT SuperMix (Cat No. E3010, New England BioLabs, Ipswich, MA), according to the manufacturer’s recommended reaction conditions (Supplemental Table 3) ...
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bioRxiv - Microbiology 2024Quote: ... RIG-I and ISG15 were determined using Luna Universal One-Step RT-qPCR Kit according to the manufacturer’s instructions (New England Biolabs). The primer sequences are available upon request ...
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bioRxiv - Microbiology 2024Quote: ... The RT RNA sample (5 μL) was amplified using the LongAmp Taq 2× Master Mix (NEB, Ipswich, MA, USA) with IVT Nanopore T7 Fw and IVT Nanopore T7 Rv primers (Supplementary Table 5) ...
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bioRxiv - Molecular Biology 2024Quote: ... All qPCRs were performed using the Luna® Universal One-Step RT-qPCR Kit (New England Biolabs, Ipswich, MA) and a Step One Plus qPCR machine (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... 461 bp DNA fragment was amplified using IRES-NADL forward and reverse primers and Q5 Hot Start polymerase (NEB, M0493S). The second round of PCR amplification extended the 461 bp fragment to include the KpnI restriction site at the 5’ end and the BamHI site at the 3’ end ...
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bioRxiv - Genetics 2021Quote: ... Multiple DNA samples were barcoded with unique dual indices using the NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (New England Biolabs) and pooled at equimolar concentrations ...
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bioRxiv - Genomics 2020Quote: ... 0.8 μM sequencing index primer and Q5 high-fidelity 1x master mix (New England Biolabs, Ipswich, MA, Cat. No. M0492) were added to perform PCR amplification ...
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bioRxiv - Molecular Biology 2019Quote: ... The DCR1 ORF along with its upstream and downstream sequence was amplified (primers: DCR1-Intergenic-For 5′-CCCCCTCGAGTTTGTAAAGAAATTGATGCTTCG; DCR1-Intergenic-Rev 5′-TGCAGGATCCGAATCTGGTATGGGATCATATTGG) and inserted between the XhoI and BamHI (NEB) restriction sites of pRS402.
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bioRxiv - Genomics 2020Quote: PCR3 was performed by subtracting 2-3 cycles from the Ct and using 2.5 uL of a mix of distinct indexed primers from the NEBNext Dual Index Kit for Illumina (New England Biolabs) using 22.5 uL PCR2 product in a 50 uL reaction volume (Q5 UltraII mastermix with EvaGreen (Biotium) ...
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bioRxiv - Synthetic Biology 2019Quote: Primers for the amplification (Eurofins Genomics, Ebersberg, Germany) were phosphorylated by the T4-polynucleotidekinase/-buffer (New England Biolabs, Ipswich, USA) prior to amplification via PCR (Q5® High-Fidelity Polymerase ...
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bioRxiv - Microbiology 2019Quote: ... The plasmid was obtained by Golden Gate cloning as follows: primers OVL1696/1697 were used to amplify pASR103 and then DpnI (NEB) treated for 1 hr at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... were amplified from genomic Synechocystis 6803 wild type DNA using specific primers (Table S1) and Phusion Polymerase (New England Biolabs). After restriction digest with BamHI and NotI ...