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Citations for New England Biolabs :
2401 - 2450 of 3130 citations for Locostatin CAS 90719 30 5 100% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... a 50 mM stock solution of pervanadate was prepared fresh by mixing equal volumes of 100 mM sodium orthovanadate (New England Biolabs) and 100 mM hydrogen peroxide ...
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bioRxiv - Microbiology 2020Quote: ... cDNA libraries with unique barcodes were generated from 100 ng enriched mRNA using NEB Next UltraII Directional RNA Library Prep kit for Illumina (New England Biolabs), then assessed for quality and quantity by Qubit ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 100 ng of gDNA was used as template for PCR using Q5 Hot Start DNA Polymerase (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Molecular Biology 2021Quote: ... rinsed gently whilst still in the slide tray three times with PBS and then treated with bleomycin (100 μM) in PBS or RNase H (100U.ml-1, NEB MO297S) in PBS supplemented with 0.1% triton ...
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bioRxiv - Microbiology 2020Quote: ... was linearized in a 500 μL reaction volume containing 100 units of ApaLI enzyme for 4-6 hours at 37°C in CutSmart buffer (New England Biolabs). The DNA was then ethanol precipitated as previously described (76) ...
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bioRxiv - Genomics 2020Quote: ... mRNA isolation was performed from 100 ng total RNA was done using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB). Exome sequencing library preparation was performed using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... beads were resuspended in GRB buffer and incubated with 1 µg of 100 bp DNA ladder (New England Biolabs # N3231S) or mononucleosome (Active motif # 81070 ...
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bioRxiv - Genomics 2021Quote: ... DNA assembly of the secondary library plasmid pool was performed with 125 ng of PCR amplicon and 825 ng of BsmBI-digested pLentiCRISPRv2 in a total reaction volume of 100 µL with HiFI DNA Assembly Mix (NEB) for 30 min at 50°C ...
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bioRxiv - Molecular Biology 2021Quote: ... containing 40 μl 50% PEG 8000 for 1 hour at room temperature then incubated overnight at 25°C in 100 μl 1x T4 RNA ligase buffer (NEB) containing 40 μl 50% PEG 8000 ...
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bioRxiv - Neuroscience 2021Quote: ... Oligos including 20 bp sgRNA sequences plus appropriate overhangs were resuspended in ddH2O at 100 μM and annealed in T4 ligase buffer with T4 polynucleotide kinase (both NEW ENGLAND BIOLABS) starting from 95 °C for 5 minutes and then temperature was ramped down to 25 °C at 5 °C/min ...
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bioRxiv - Plant Biology 2023Quote: ... from 100 to 1000 ng of total RNA using a stranded kit (Illumina TruSeq Stranded Total RNA Kit or NEB Next Ultra™ II Directional RNA Library Prep Kit for Illumina) ...
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bioRxiv - Neuroscience 2022Quote: ... 100 cells were isolated directly into low protein binding eppendorfs containing 2 ul NEBNext Single Cell Lysis Buffer (NEB, E5530S). Samples were kept on dry ice until transfer to −80 C for overnight storage.
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bioRxiv - Developmental Biology 2023Quote: ... 25 uL of 2xphusion PCR mastermix (Phusion High-Fidelity PCR Master Mix with HF Buffer – 100 rxns, NEB, cat #M0531S), 0.625uL of 100uM F primer ...
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bioRxiv - Molecular Biology 2023Quote: ... Harvested cells were rinsed with ice-cold PBS and resuspended in lysis buffer with 1% Triton X-100 and 40 U/mL murine (New England Biolabs) for 20 minutes with intermittent tapping on ice ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 μg of cDNA was amplified with Phusion polymerase for 32 cycles and separated on a standard 1% agarose gel together with a 100 bp or 1 kb ladder (New England Biolabs). Ribosomal protein L32 (RpL32 ...
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bioRxiv - Cancer Biology 2023Quote: ... lysate volume equivalent to 20-40 µg total protein was first denatured at 100°C for 10 min in 1X glycoprotein denaturation buffer (NEB). Endo H digestion was performed at 37°C for 1h in the presence of 1X GlycoBuffer 3 and 1 µL of Endo H enzyme (NEB) ...
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bioRxiv - Genetics 2023Quote: ... libraries were generated using total RNA from single and pooled mites (100-600 ng) with the NEBNext Multiplex Small RNA Library Prep kit (NEB) following the manufacturers protocol ...
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bioRxiv - Biochemistry 2023Quote: ... The reserved half of the stored fixed cells were pelleted and resuspended in 100 μL Klenow extension buffer (1X NEB buffer 2 ...
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bioRxiv - Cell Biology 2023Quote: ... To remove RNA secondary structure and anneal the mRNA capture primer 1 μL of tagged random hexamer (100 μM) and 0.5 μL of 10 mM dNTPs (dNTP solution set NEB - N0446S) were added to the sample ...
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bioRxiv - Cancer Biology 2023Quote: ... Total amount of 100 ng of small RNA or mRNA was digested with a Nucleoside Digestion Mix (New England BioLabs) according to the manufacturer’s instruction ...
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bioRxiv - Genomics 2023Quote: ... The nuclei were pelleted by centrifuging at 500 g for 5 min at 4 °C and resuspended in 200 μl 1X NEBuffer 2.1 (50 mM NaCl, 10 mM Tris-HCl, 10 mM MgCl2, 100 μg/ml BSA; NEB, B7202) in a 1.5 ml tube ...
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bioRxiv - Molecular Biology 2022Quote: ... The end-repaired cDNA was ligated with 2 μL barcoded adaptor (100-466-000, Pacific Biosciences) with T4 DNA Ligase (M0202, New England Biolabs) in 50 μL reaction volume at room temperature for 1 hour ...
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bioRxiv - Molecular Biology 2024Quote: The golden gate assembly reaction was assembled in a PCR tube by mixing 100 ng of the vector with a 3 fold molar excess of the purified PCR product (calculated using the NEB bio calculator ...
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bioRxiv - Biochemistry 2024Quote: ... 4SU-labeled RNA (100 µg) was biotinylated with HPDP-biotin (cat. 21341, ThermoScientific) and captured on 150 µL of streptavidin magnetic beads (NEB) per RNA sample ...
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bioRxiv - Molecular Biology 2024Quote: ... The biotin labeled nuclei were washed once with 1x NEB ligation buffer and resuspended with ligation mix (100 ul 10x NEB ligation buffer ...
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bioRxiv - Neuroscience 2024Quote: ... and 0.5% (vol/vol) Triton X-100 in nuclease-free water and 1% (vol/vol) proteinase K (New England Biolabs, P8107S). The sample was digested in this buffer for ≥36h in a humidified ...
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bioRxiv - Genomics 2024Quote: ... Genomic DNA was harvested by discarding culture media and adding lysis buffer consisting of 20 mM Tris pH 8 and 0.1% Triton X-100 (MilliporeSigma T9284) with 60 ng/mL of Proteinase K (New England Biolabs P8107S) added immediately prior to use ...
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bioRxiv - Cell Biology 2024Quote: ... 100 fmol of library per 50 µL PCR reaction was amplified using Q5 HotStart DNA Polymerase (New England Biolabs M0493S) in 8 reactions with a 50 °C – 62 °C gradient annealing step using the following program:
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bioRxiv - Immunology 2024Quote: Reverse-transcription on total RNA (100 to 500 ng) was performed with random hexamer/oligo-dT-Primer containing LunaScript RT Supermix (New England Biolabs) following the manufactureŕs instructions ...
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bioRxiv - Microbiology 2024Quote: ... reactions were performed in a final volume of 100 μl using the PURExpress ΔRibosome In vitro Protein Synthesis Kit (New England Biolabs) according to the manufacturer instructions and with ribosomes purified from E ...
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bioRxiv - Developmental Biology 2023Quote: ... 100 ng of total intact RNA was used with the NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB, #E7490S), and the enriched mRNA was prepped for sequencing with the NEBNext® Ultra™ II Directional RNA Library Prep with Sample Purification Beads (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation (15 min, 12000 x g) and 100 units of λ-phosphatase (#P0753, New England Biolabs) were added to the supernatants ...
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bioRxiv - Plant Biology 2023Quote: ChIP-seq libraries were constructed from 100 ng of DNA samples using the NEB Ultra II DNA Library Prep Kit for Illumina (New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... libraries were prepared for all samples following the epiGBS2 protocol42,43 by digesting 100 ng of gDNA with restriction enzymes NsiI and Csp6I (New England Biolabs, UK). Individually barcoded hemimethylated adapters ...
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bioRxiv - Microbiology 2023Quote: ... biwaensis Akk2750 (100% sequence match to CSUN-19) (38) with Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs) and incorporating a C-terminal HA epitope tag ...
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bioRxiv - Molecular Biology 2023Quote: ... 1× CutSmart buffer (50 mM Potassium Acetate, 20 mM Tris-acetate, 10 mM Magnesium Acetate, 100 µg/ml BSA or recombinant albumin; NEB), 0.5 mM 1,4-Dithiothreitol (DTT) ...
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bioRxiv - Molecular Biology 2023Quote: ... sgRNA amplicons were generated by PCR of 2 ug genomic DNA per 100 ul reaction with Q5 2X Hot Start Master Mix (M0494L, NEB) and enough reactions to maintain 1000x screening sgRNA representation ...
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bioRxiv - Biophysics 2023Quote: ... The reaction was mix using 1× CutSmart buffer (50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate, and 100 μg/mL BSA; NEB) and 0.5 mM DTT (1,4-dithiothreitol ...
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bioRxiv - Bioengineering 2023Quote: ... genomic loci were amplified in a first PCR reaction (PCR-1) reaction from approximately 100 ng of gDNA using Q5 High-fidelity DNA Polymerase (NEB) and the primers listed in Sup ...
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bioRxiv - Cell Biology 2023Quote: ... Two 100 μl aliquots were incubated overnight at 37°C with and without 1 U Endo H (NEB, Frankfurt, Germany). Then the samples were heated with 100 μl SDS sample buffer for 5 min at 95°C.
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bioRxiv - Microbiology 2024Quote: The msdDNA cDNA was isolated from acrylamide gels and 100 ng was used to extend the 3’ end with dCTP or dGTP and terminal deoxynucleotidyl transferase (TdT) from NEB, according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... 15 µl of genomic DNA (DNA concentration is around 100 ng/µl) were digested or mock with 20 units of BsrGI or BamHI restriction enzymes (New England BioLabs) for 5 hours at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... Library preparation was conducted by the standard ≥100 ng protocol from the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB) with minor modifications ...
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bioRxiv - Genomics 2022Quote: ... Dam activity was induced by resuspension in 100 μL DigWash supplemented with 80 μM S-adenosyl-methionine (SAM) (NEB, B9003S) and incubated at 37 °C for 30 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... 100 ng of each gel-purified PCR products (total of 19) were mixed and digested with BsmBI restriction enzyme (NEB) for 2 h at 55 °C ...
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bioRxiv - Biochemistry 2022Quote: BsmBI-digested vector and insert were ligated in a molar ratio of 1:100 to a total of 1 μg using T4 DNA ligase (NEB) for 2 h at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions between 100–400 nt were cut and DNA was extracted from the gel by Monarch DNA Gel extraction Kit (NEB). Multiplexed samples were submitted to the IMG Genomics and Bioinformatics facility for library synthesis using the NextSeq® 500/550 High Output Kit v2 ...
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bioRxiv - Microbiology 2022Quote: ... and cDNA libraries containing unique barcodes generated from 100 ng mRNA using the Next UltraII Directional RNA Library Prep kit for Illumina (New England Biolabs). The individual cDNA libraries were assessed for quality and quantity by Qubit ...
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bioRxiv - Molecular Biology 2023Quote: ... increasing volumes of SPRI beads were mixed with 1 μl of a 4-fold dilution of 100 bp DNA ladder (New England Biolabs) and diluted to a final volume of 100 μl with 10 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was eluted at 100% Buffer F and protein fractions were pooled and combined with TEV and CIP (#M0525) from NEB and dialyzed overnight against Buffer E at 4 °C ...