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Citations for New England Biolabs :
2301 - 2350 of 10000+ citations for rno mir 542 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... PCR reactions were performed with Q5 polymerase (New England Biolabs) in the buffer provided ...
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bioRxiv - Biophysics 2023Quote: ... The reverse-transcribed DNA was PCR amplified using Phusion (NEB) for 27 cycles according to the manufacturer’s instruction using primers miR31_buffer_F and miR_tail_RT (Table S6) ...
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bioRxiv - Cancer Biology 2022Quote: ... PCR using Q5 High-Fidelity DNA Polymerase (New England Biolabs) was used to amplify gBlocks and PCR products were purified using Nucleospin Gel and PCR Cleanup kit following manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... PCR was performed using Phusion High-Fidelity DNA Polymerase (NEB) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: ... PCRs were digested with DpnI (New England Biolabs [NEB], R0176S), purified using a gel DNA recovery kit (Zymo Research ...
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bioRxiv - Cell Biology 2023Quote: ... PCRs were digested with DpnI (New England Biolabs [NEB], R0176S), purified using a gel DNA recovery kit (Zymo Research ...
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bioRxiv - Microbiology 2023Quote: ... raw PCR product was mixed with T4 DNA ligase (NEB), DpnI (NEB) ...
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bioRxiv - Genomics 2023Quote: ... The PCR product is digested by the enzyme DPN1 (NEB) in order to remove the template plasmid and cloned in TOPO10 E ...
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bioRxiv - Evolutionary Biology 2023Quote: ... each exon was subjected to PCR (NEB OneTaq®, M0480) and sequencing (University of Utah ...
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bioRxiv - Molecular Biology 2023Quote: ... and NEBNext High-Fidelity 2X PCR Master Mix (NEB M0541S). All DNA clean-up steps were performed with AMPure XP beads (Beckman Coulter A63880) ...
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A modular circuit architecture coordinates the diversification of courtship strategies in DrosophilabioRxiv - Neuroscience 2023Quote: ... were PCR amplified with Q5 High-Fidelity master mix (NEB) and cloned into pCFD4 (Addgene 49411 ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was obtained using reverse transcription PCR (Yugong Biolabs, China). The reaction system was prepared according to the kit’s requirements (Yugong Biolabs ...
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bioRxiv - Synthetic Biology 2024Quote: ... Titers were determined by quantitative PCR on DNase I (NEB) treated and proteinase K (Qiagen ...
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bioRxiv - Neuroscience 2024Quote: ... using fragments obtained from PCR with Q5 or Phusion (NEB) or Platinum SuperFi DNA Polymerase (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... All PCR reactions used Q5® High-Fidelity polymerase (NEB) unless otherwise specified ...
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bioRxiv - Microbiology 2023Quote: ... High-fidelity PCR amplification was performed using Phusion polymerase (NEB), and standard PCR amplification was performed using Taq mix red (PCRBIO) ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 µL PCR reaction contained 1× OneTaq Master Mix (NEB), 0.2 µM of each primer ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR was done using Q5 High-Fidelity DNA polymerase (NEB) and Monarch PCR Cleanup Kit (NEB ...
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bioRxiv - Microbiology 2023Quote: ... The PCR products were treated using DpnI (New England Biolabs) and purified using the Wizard SV Gel system and the PCR Clean-Up System (Promega).
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bioRxiv - Biochemistry 2024Quote: ... were generated by PCR using λ DNA template (NEB, N3011S)31 ...
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bioRxiv - Cell Biology 2023Quote: ... PCR reactions were performed with Q5 polymerase (New England Biolabs) in the buffer provided ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were digested with 1 μL DpnI (NEB #R0176L) for 1 hour and purified by agarose gel extraction (MinElute Gel Extraction Kit ...
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bioRxiv - Synthetic Biology 2024Quote: ... followed by purification with Monarch® PCR & DNA CleanupKit (NEB). Successful DNA assembly was verified first by colony PCR using GoTaq® Green Master Mix (Promega ...
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bioRxiv - Immunology 2024Quote: ... The products from these PCR reactions were purified (NEB T1030S). The purified products were sent to Eurofins for Sanger sequencing ...
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bioRxiv - Genomics 2024Quote: ... The PCR products were treated with Exonuclease 1 (NEB, M0293S) to digest single-stranded products ...
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bioRxiv - Microbiology 2024Quote: ... All PCR-generated probes were treated with exonuclease I (NEB) according to the manufacturer’s protocol to remove single-stranded DNA ...
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bioRxiv - Systems Biology 2024Quote: ... The PCR products were digested with DpnI (New England Biolabs) at 37°C for 1 hour to remove the original methylated template ...
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bioRxiv - Genetics 2024Quote: Histone array PCR amplicons were incubated with PaqCI (NEB #R0745) for 1-3 hours at 37C and subsequently cleaned up with GeneJet PCR clean up kit (Thermo Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... PCR reactions were performed with Q5 polymerase (New England Biolabs) in the buffer provided ...
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bioRxiv - Molecular Biology 2024Quote: ... Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB) following the manufacturer’s instruction and specific primers (see primer list) ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified PCR products were digested with NotI (New England Biolabs) and ligated into pMON721 linearized with NotI to generate the SEGS-1 G ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product was treated with DpnI (New England Biolabs). All plasmids were transformed into E ...
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bioRxiv - Microbiology 2024Quote: ... All PCR steps were carried out with Phusion polymerase (NEB) using the manufacturer’s guidelines.
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bioRxiv - Microbiology 2024Quote: ... Large colonies were screened with colony PCR (Phusion polymerase, NEB) for integration through the 5’ flank using primers P1 and P6 ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR products were digested with Dpn1 (NEB, Ipswich, MA, USA) to destroy the original template and were purified using the QIAquick Gel Extraction Kit (Qiagen ...
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bioRxiv - Synthetic Biology 2024Quote: ... All reagents for cloning and PCR were purchased from NEB and Fisher Scientific.
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bioRxiv - Cell Biology 2023Quote: ... Protein samples were resolved on an 12% SDS-polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membrane for detection with the following antibodies: anti-Glucocorticoid Receptor D6H2L (1:1000 dilution, Cell Signaling, New England BioLabs #12041), anti-PAX7c (1:500 dilution ...
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bioRxiv - Developmental Biology 2021Quote: ... DNA was amplified with 12 cycles of PCR using the NebNext Hi-Fi 2X PCR Master Mix (New England Biolabs Inc, Ipswich, MA, Cat #M0541S). Following PCR ...
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bioRxiv - Genomics 2020Quote: ... sticky ends were filled by incubating the nuclei for 4h at RT with DNA Polymerase I (New England Biolabs, Cat. N: M0210) and a biotin-14-dATP (Life Technologies ...
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bioRxiv - Genomics 2020Quote: ... Adapter ligation (AMX) was performed at RT (20 °C) for 20 minutes using NEB Quick T4 DNA Ligase (New England Biolabs, MA, USA). The reaction mixture was purified using 0.6X AmPure beads (Beckmann Coulter ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative PCR was performed by transferring 2 μl of the RT mix to the qPCR mix prepared with Luna Universal qPCR Master Mix (M3003, New England Biolabs, MA, USA), according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: ... Reverse transcription and qPCR were performed in a one-step reaction using Luna® Universal One-Step RT-qPCR (NEB, Ipswich MA) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Adapter ligation (AMX) was performed at RT (20 °C) for 20 min using NEB Quick T4 DNA ligase (New England Biolabs, MA, USA). The reaction mixture was purified using 0.6X AmPure beads (Beckman Coulter ...
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bioRxiv - Biochemistry 2024Quote: ... Two-hundred and fifty ng of RNA were used for reverse transcription reactions using the LunaScript RT SuperMix (New England BioLabs cat# E3010). For the qPCR ...
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bioRxiv - Immunology 2024Quote: ... cDNA for 3’ RACE was generated using RNA from IFN-β treated A549 cells using the Template Switch RT Enzyme Mix (NEB, #M0466) and anchored primers ...
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bioRxiv - Biophysics 2021Quote: Time-resolved experiments with TEV protease were performed by adding 1 μL TEV protease (New England Biolabs) after the 3rd time point to an imaging well containing 100 μL protein sample ...
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bioRxiv - Microbiology 2020Quote: ... The gene was assembled at the same time as recombination into the vector using infusion technology (NEB) and the assembly reaction used to transform E.coli (NEB 10 beta) ...
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bioRxiv - Genetics 2019Quote: ... After washing the pellet two times with 1ml of 1 × NEB buffer 2.1 (New England BioLabs (NEB), USA) ...
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bioRxiv - Genetics 2019Quote: ... After washing the pellet two times with 1ml of 1 × NEB buffer 2.1 (New England BioLabs (NEB), USA) ...
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bioRxiv - Genomics 2021Quote: ... and washed several times before being resuspended the beads in 20 µl NEBuffer 2 (New England Biolabs). The final library was generated using the Illumina TruSeq kit (Illumina ...