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Citations for New England Biolabs :
2301 - 2350 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were purified using Monarch PCR & DNA Cleanup Kit (5 μg; NEB) and checked on 1% agarose gel and approximately 600 ng of each PCR product was used as template for in vitro dsRNA synthesis according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids were extracted from each strain by miniprep kit (New England Biolabs, T1010L) and whole plasmid sequencing was performed by Plasmidsaurus using Oxford Nanopore Technology with custom analysis and annotation ...
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bioRxiv - Biochemistry 2024Quote: ... The total RNA was reverse transcribed using the LunaScript RT SuperMix Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Ribosomal RNA was depleted using the NEBNext rRNA Depletion Kit v2 (NEB; E7400L) and libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Microbiology 2024Quote: ... NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England BioLabs) and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs ...
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bioRxiv - Microbiology 2024Quote: ... The DNA was then purified using the Monarch PCR & DNA Cleanup Kit (NEB), and RNA was synthesised from the purified DNA using the MEGAscript T7 Transcription Kit (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... Alkylated RNA was purified with the Monarch RNA Cleanup Kit (New England Biolabs). RNA quality was confirmed after each purification with a TapeStation 4150 (Agilent) ...
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bioRxiv - Molecular Biology 2024Quote: ... Quick change was performed using Q5® Site-Directed Mutagenesis Kit (NEB: E0554S) to mutate the lysines at position 26-27 to glutamines or alanines ...
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bioRxiv - Biochemistry 2024Quote: ... An Illumina-compatible library was prepared with NEB Ultra II DNA kit (NEB) and each library was shotgun sequenced with Illumina HiSeq3000 (2 x 150 bp ...
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bioRxiv - Genomics 2024Quote: ... and used for Illumina library using the NEBNext ultra II kit (NEB E7600). Briefly ...
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bioRxiv - Plant Biology 2024Quote: ... The NebNext Ultra II RNA library prep kit (New England Biolabs, MA, USA) was used to synthesize RNA libraries ...
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bioRxiv - Microbiology 2024Quote: ... The amplified PCR products were ligated using a Gibson assembly kit (NEB, USA). The resulting plasmid pLZ asnA R100K was electroporated into strain S119 ΔasnA for expression of mutated asnA.
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bioRxiv - Neuroscience 2024Quote: Constructs were all generated using the Gibson Assembly Cloning Kit (New England Biolabs) by inserting the different DNA fragments (PCR-generated or gBlocks ...
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bioRxiv - Genomics 2024Quote: ... PCR products were purified using a Monarch PCR & DNA Cleanup Kit (NEB, T1030S) following the Manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs) was used for the following RNA-seq library preparation according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Total RNA was isolated using NEB Monarch RNA prep kit (New England BioLabs). The RNA concentration and RIN (RNA integrity number ...
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bioRxiv - Cell Biology 2024Quote: ... PCR products were purified using Monarch DNA Gel Extraction Kit (New England Biolabs). Both the vector and the complete insert were digested by BsmBI (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... before purifying again using an NEB RNA clean and concentrate kit (NEB #T2030).
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bioRxiv - Synthetic Biology 2024Quote: ... Selections were then purified using the Monarch® PCR & DNA Cleanup Kit (NEB), eluting in 10 µl of the Monarch® DNA Elution Buffer ...
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bioRxiv - Genomics 2024Quote: ... The DNA was then purified using the Monarch PCR & DNA Cleanup Kit (NEB). The first-dimension electrophoresis was run in 0.7% agarose in 1xTBE buffer at 0.7 V/cm for 18h ...
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bioRxiv - Genomics 2024Quote: ... gDNA extraction was performed using a Monarch Genomic DNA Purification Kit (NEB, T3010S) following the Manufacturer’s protocol for cultured cells with an input of 5e6 HeLa cells ...
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bioRxiv - Genomics 2024Quote: ... followed by purification with a Monarch® PCR & DNA Cleanup Kit (NEB®). Standard experimental conditions ...
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bioRxiv - Genomics 2024Quote: ... Further DNA was purified using Monarch® Genomic DNA Purification Kit (NEB T3010S) according to the manufacturer’s instructions with minor modifications ...
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bioRxiv - Developmental Biology 2024Quote: ... and quantified using a NEBNext® Library Quant Kit for Illumina® (NEB) on a QuantStudioTM 5 Real-Time PCR System (ThermoFisher ...
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bioRxiv - Microbiology 2024Quote: ... Ribosomal RNA was depleted using the bacteria rRNA depletion kit (New England Biolabs), with the addition of human rRNA depletion beads for the samples containing S ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR products were purified using a Monarch PCR Cleanup Kit (New England Biolabs) and prepared for nanopore long read sequencing using the Ligation Sequencing Kit (Oxford Nanopore Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... and mutated using the Q5 site-directed mutagenesis kit (New England Biolabs, E0554S). Modified sgRNAs targeting Gria3 were synthesized by Synthego (see Table for sgRNA and donor sequences) ...
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bioRxiv - Synthetic Biology 2024Quote: ... and purified using the Monarch PCR and DNA Cleanup Kit (New England Biolabs). 200 ng of purified product was used in a 20 µL IVT reaction using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) ...
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bioRxiv - Molecular Biology 2024Quote: ... Amplicons were cleaned up using Monarch PCR & DNA cleanup kit (catalog #: T1030S, NEB). Sequences for the mutant pFmiR-CMAS sensors were verified by sequencing and used in the miRFluR assay as described previously ...
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bioRxiv - Microbiology 2024Quote: ... or the Monarch Total RNA Miniprep Kit from New England Biolabs (NEB #T2010). For measurements of cell-free viral populations ...
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bioRxiv - Microbiology 2024Quote: ... and re-ligated using the Quick Ligation Kit (NEB, Frankfurt am Main, Germany) before transformation into E ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by purification using the Monarch® RNA Cleanup Kit (New England Biolabs).
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bioRxiv - Molecular Biology 2024Quote: RNA from HEK293T cells was purified using Monarch total RNA miniprep kit (NEB). cDNA was reverse-transcribed with SuperScript III (Invitrogen) ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR reactions were cleaned up with the Monarch PCR & DNA Cleanup kit (NEB), with product size and integrity verified by agarose gel electrophoresis ...
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bioRxiv - Microbiology 2024Quote: ... iPGAM was mutated with the Q5® Site-Directed Mutagenesis Kit (NEB, E0554).
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bioRxiv - Genomics 2024Quote: ... gDNA extraction was performed using a Monarch Genomic DNA Purification Kit (NEB, T3010S) following the Manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... PCR products were purified using a Monarch PCR & DNA Cleanup Kit (NEB, T1030S) following the Manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Libraries were prepared with the NEBNext Ultra II DNA library prep kit (NEB) reagents according to the manufacturer’s protocol on the beads suspended in 25μL TT buffer ...
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bioRxiv - Microbiology 2024Quote: ... RNA wash buffer (500 µl) from Monarch Total RNA Miniprep Kit (NEB #T2010) was added ...
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bioRxiv - Microbiology 2024Quote: ... All ligations were performed using NEBuilder HiFi DNA Assembly kit (New England Biolabs). Recombinant pCVD442 plasmids were transformed into E ...
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bioRxiv - Microbiology 2024Quote: ... Phage genomic DNA was extracted using Monarch HMW DNA extraction kit (NEB #T3060) following the manufacturers guidelines ...
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bioRxiv - Neuroscience 2024Quote: ... We then transcribed sgRNA in vitro using the HiScribe T7 Kit (NEB, E2040S) with an 8-9 hr incubation at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was isolated using the Monarch Total RNA Miniprep Kit (NEB, T2010S). The qRT-PCR assay was performed using 500 ng of total RNA and the Luna Universal One-Step RT-qPCR Kit (NEB ...
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bioRxiv - Plant Biology 2024Quote: RNA was extracted using the Monarch Total RNA Miniprep Kit (New England Biolabs), according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: Using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, E2040), the sense strand was amplified further and we then produced antisense ssDNA by reverse transcription (RT ...
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bioRxiv - Neuroscience 2024Quote: ... RNA extraction was performed using the Monarch total RNA Miniprep Kit (NEB, T2010S) following the manufacture recommendations and RNA samples were stored at -80°C until use.
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bioRxiv - Synthetic Biology 2024Quote: ... expression was performed using the PURExpress® In Vitro Protein Synthesis Kit (NEB). For the non-T7 constructs ...
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bioRxiv - Immunology 2024Quote: ... We then performed Gibson assembly using the Gibson Assembly Kit (New England Biolabs) on the digested backbone and synthesized DNA blocks (Codex DNA ...
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bioRxiv - Synthetic Biology 2024Quote: ... These modifications were introduced using the Q5 Site-Directed Mutagenesis Kit (NEB, E0554S), following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: Monarch PCR & DNA Cleanup Kit (New England Biolabs Japan Inc., Tokyo, Japan, #T1030)