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Citations for New England Biolabs :
2251 - 2300 of 5045 citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade C 16055 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and digested by proteinase K (New England Biolabs, 1:100 dilution to 8 units ml−1) overnight at 37°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... Reagents added were 1 μl of 1 mg/ml BSA (diluted from 20 mg/ml - NEB), 1 μl T4 DNA ligase buffer (ThermoFisher/NEB) ...
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bioRxiv - Biochemistry 2020Quote: ... A pre-cleaning step was applied by incubating each sample with 10 μl of Protein A magnetic beads (New England Biolabs GmbH, Frankfurt, Germany) for 1 h at 4 °C in tube roller (15 rpm ...
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bioRxiv - Cell Biology 2022Quote: ... The DNA encoding the EGFP-fusion proteins and the P2A peptide was inserted by Gibson assembly using NEBuilder HiFi DNA assembly master mix (New England BioLabs, Ipswich, MA, USA). The HeLa Flp-In T-REx K44A Dynamin2-EGFP-P2A-Caveolin1-mCherry construct was obtained by in vitro mutagenesis of the pcDNA/FRT/TO/Dyn2-EGFP-P2A-Caveolin1-mCherry construct exchanging lysine at position 44 to an alanin using these primers ...
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bioRxiv - Developmental Biology 2020Quote: ... A total of 863 Bicyclus embryos were injected with 300 ng/µl of guide and 300 ng/µl of Cas9 protein (NEB; Cat. no.: M0641) mixed together in equal parts (total volume = 10 µl ...
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bioRxiv - Developmental Biology 2019Quote: ... The bound protein (~42kDa, 360 amino acids) was released from the MBP tag by cleavage with Factor Xa (New England Biolabs, Massachusetts, United States) for 24 hours at 4°C in 20mMTris pH 7.2 ...
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bioRxiv - Biochemistry 2021Quote: ... and one plasmid was randomly selected from those plasmids and used for expression with a PURE system (PURExpress In Vitro Protein Synthesis Kit, New England BioLabs, Ipswich, MA, USA).
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bioRxiv - Cancer Biology 2020Quote: ... The synthetic genes encoding WT human TRIM28 with an N-terminal maltose binding protein (MBP) affinity tag (UniProt: Q13263) were subcloned to the pMAL-p2X vector (New England Biolabs, Beverly, MA, USA).
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bioRxiv - Neuroscience 2023Quote: ... Samples were diluted to 1.0 mg/mL protein using homogenization buffer and incubated with 20 U/μL lambda phosphatase in MnCl2 and enzyme buffer as supplied with the lambda protein phosphatase kit (New England Biolabs, Evry-Courcouronnes, France) for 3 h at 30 °C ...
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bioRxiv - Cell Biology 2023Quote: ... corresponding to a portion of PfRNF1 (RP2; Fig. 1A) was expressed as a maltose-binding fusion protein using the pMAL™c5X-vector (New England Biolabs, Ipswich, USA). To this end ...
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bioRxiv - Cancer Biology 2021Quote: ... and 1 µL T4 PNK (NEB) followed by incubation for 60 min at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μl (10U) T7 endonuclease (NEB) were added to the hybridized PCR products and incubated for 15min at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 µl rSAP (NEB, Cat M0371) was then added to dephosphorylate 3’ end of DNA at 37℃ for 1hr ...
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bioRxiv - Microbiology 2022Quote: ... and 1 µl 5’deadenylase (NEB) at 30°C for 30 min and removed by RNA Clean & Concentrator (Zymo Research) ...
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bioRxiv - Microbiology 2022Quote: ... 1 µl of rSAP enzyme (NEB) and 1 µl of PNK enzyme (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 x protease inhibitor cocktail (NEB), 2 mM N-ethylmaleimide (NEM) ...
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bioRxiv - Molecular Biology 2021Quote: ... in 1X NEBuffer 1 (NEB #B7001) for 15 min at 37°C while shaking at 1000 rpm for an interval of 15 sec every 3 min ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μl β-agarase (NEB M0392S) was added and mixed by flicking without allowing sample to cool ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μl β-agarase (NEB M0392S) was added and mixed by flicking without allowing sample to cool ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μL murine RNase inhibitor (NEB), and 1 μL of 200 U/μL SuperScript III reverse transcriptase (Invitrogen).
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bioRxiv - Molecular Biology 2021Quote: ... 1 µl 10x Taq buffer (NEB), 0.2 µl 10 mM dNTPs (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 µl 10x Vent buffer (NEB), 0.2 µl 10 mM dNTPs (NEB) ...
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bioRxiv - Genomics 2022Quote: ... 1 μl rSAP enzyme (NEB, M0371L) and 1 μl PNK enzyme (NEB ...
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bioRxiv - Genomics 2022Quote: ... added 1 μl RecJf (NEB, M0331) and 1 μl 5’deadenylase (NEB ...
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bioRxiv - Genomics 2020Quote: ... we added 1 μlExonuclease I (NEB), 1 μl Exonuclease III (NEB) ...
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bioRxiv - Microbiology 2020Quote: ... HiFi Polymerase (1 unit, Phusion, NEB) in GC buffer with MgCl2 (NEB ...
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bioRxiv - Genomics 2020Quote: ... 1 μl T4 DNA Polymerase (NEB) and 5 μl Large (Klenow ...
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bioRxiv - Genomics 2020Quote: ... in 1X NEBuffer 1 (NEB #B7001) at 37°C for 15 minutes ...
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Genetic mapping of fitness determinants across the malaria parasite Plasmodium falciparum life cyclebioRxiv - Evolutionary Biology 2019Quote: ... 1 mM dNTPs (New England Biolabs), 3.5 μM of each amplification primer (Oyola et al ...
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bioRxiv - Molecular Biology 2019Quote: ... or T4 RNA-ligase 1 (NEB), respectively ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 U of Taq polymerase (NEB), 0.25 μM forward primer ...
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bioRxiv - Genomics 2019Quote: ... 1 μl T7e1 enzyme (NEB, USA) was added and incubated at 37°C in a water bath for 15 min ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1 µL T4 Ligase Buffer (NEB) and 0.5 µL BsaI (10000 U/mL ...
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bioRxiv - Genomics 2020Quote: ... 1 µL 10x Ligase Buffer (NEB) and 1 µL T4 DNA Ligase (NEB ...
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bioRxiv - Genomics 2021Quote: ... 1 mM dNTPs (New England Biolabs), 2.5 μM of each amplification primer (Additional file 1 ...
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bioRxiv - Genetics 2020Quote: ... and sodium fluoride (NEB, 1 mM). 150uL of ice cold buffer was added to heads followed by immediate homogenization with a motorized pestle for 10 seconds on ice ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM dNTPs (New England Biolabs), 6.67 mM DTT (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2020Quote: ... 1 mM ATP (New England Biolabs), filled to 10 μL with dH2O prepared on ice ...
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bioRxiv - Bioengineering 2020Quote: ... 1 mM ATP (New England Biolabs), filled to 100 μL with dH2O prepared on ice ...
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bioRxiv - Bioengineering 2020Quote: ... 1 mM of each rNTP (NEB), and 9 mM magnesium chloride ...
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bioRxiv - Genetics 2020Quote: ... 1 μl DNA polymerase Klenow (NEB), and 5 μl T4 PNK (NEB M0201 ...
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bioRxiv - Cell Biology 2021Quote: ... and CLIPcell-TMR (1 µM; NEB) where appropriate ...
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bioRxiv - Biochemistry 2021Quote: ... 1 U/μL RNase Inhibitor (NEB)] for 20 minutes at RT before loading onto a 1 mm thick non-denaturing polyacrylamide gel (6% ...
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bioRxiv - Biochemistry 2020Quote: ... 1 μl of T4 PNK (NEB), and 14 μl of dH2O for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... Primer Set 1 (New England Biolabs). Libraries were sequenced on an Illumina HiSeq 2500 instrument using 50-bp single-end reads.
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bioRxiv - Synthetic Biology 2021Quote: ... 0.5 units μL−1 PstI (NEB) and 7.5 μM DFHBI (Tocris) ...
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bioRxiv - Systems Biology 2021Quote: ... 1 μL of Phusion (NEB M0530S), 1X of Phusion Buffer ...
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bioRxiv - Genomics 2021Quote: ... 1 mM dNTPs (New England Biolabs), 3.5 μM of Phi29 Random Hexamer Primer ...
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bioRxiv - Microbiology 2021Quote: ... and 1U XRN-1 (NEB, USA) in NEB3 Buffer for 1h at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... using T4 RNA ligase 1 (NEB). RNA samples were pooled and treated with RiboZero (Gram-Negative Bacteria ...