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Citations for New England Biolabs :
2101 - 2150 of 5045 citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade C 16055 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Purified protein (4 μg) was deglycosylated with 500 U of Endoglycosidase H (Endo H) (New England Biolabs, Ipswich, MA, United States) according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2023Quote: ... Strep–PIF4 recombined proteins were expressed in the Escherichia coli BL21 (DE3) strain and then purified using amylose resin (NEB, E8021S). DNA probes of NHX1 and SAL1 were synthesized and labeled using the Biotin 3′ End DNA Labeling Kit (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... the particles were captured by magnetic stand and proteins captured by the particles were separated by SDS-PAGE and examined by immunoblotting using MBP antibody (NEB, #E8032S). For the immunoblot ...
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bioRxiv - Biochemistry 2024Quote: ... the crude extract containing 100 μg (in 100 μL volume) of proteins was treated with 400 units of λ-PPase (#P0753; New England Biolabs) for 60 min at 30 °C ...
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bioRxiv - Biophysics 2023Quote: Tau was phosphorylated in vitro according to previous protocols with some modifications.12,88 50 µL of 100 µM tau was incubated with 2 µg cAMP-dependent Protein Kinase (PKA, New England BioLabs P6000S) and/or 0.4 µg GSK3β (Sigma-Aldrich G4296 ...
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bioRxiv - Cell Biology 2024Quote: All purified SNAP-tagged proteins were labeled with 10-fold molar excess of SNAP-Surface dyes (New England Biolabs, Ipswich, MA) in labeling buffer (1× PBS ...
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bioRxiv - Synthetic Biology 2023Quote: ... we constructed the genetic sequence of the major shell protein of 50nmGVs using Q5 DNA polymerase and Gibson assembly kits (New England Biolabs Inc.)65 ...
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bioRxiv - Microbiology 2023Quote: ... Each protein was then isolated and affinity-purified using an amylose resin according to the manufactureŕs protocol (New England Biolabs, Ipswich, US) and the concentration was determined using the Bradford technique ...
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CXCL17 binds efficaciously to glycosaminoglycans with the potential to modulate chemokine signallingbioRxiv - Immunology 2023Quote: ... WT SUMO3-CXCL17 and variants were expressed as soluble proteins in SHuffle® T7 Competent E.coli (New England Biolabs, Hitchin, UK). In both protocols ...
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bioRxiv - Cell Biology 2023Quote: ... The protein was then purified by affinity-purification using an amylose beads according to the manufacturer’s protocol (New England Biolabs, Ipswich, USA) and the concentration determined by Bradford assay ...
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bioRxiv - Cell Biology 2023Quote: Beads with bound Flag-RAD50 or GFP-BRCA1 were washed with 5M NaCl to eliminate contamination with protein kinases and incubated with 100 or 500 U of CK2 holoenzyme complex (α2/β; NEB) in 30 μl of kinase buffer (20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Molecular Biology 2023Quote: ... Unligated 3’ linker was removed by incubating the samples with the 5’-deadenylase KmHnt3 (see Recombinant protein expression and purification) and RecJ exonuclease (New England Biolabs, M0264S) for 45 min at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: The cell lysates or purified PINK1 bound to FLAG beads were incubated at 30°C for 1h with 200 units of ʎ protein phosphatase (LPP) in a total reaction of 100µL following the instructions of the manufacturer (New England Biolabs #P0753).
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bioRxiv - Cell Biology 2024Quote: We fused SNAP-3xHA coding sequences downstream H3.1 and H3.3 CDS 46 and inserted this fusion protein into the pBS31 vector 70 using NEBuilder® HiFi DNA Assembly Master Mix (NEB). These expression vectors (pB31-H3.1- and pB31-H3.3-SNAP-3xHA ...
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bioRxiv - Neuroscience 2021Quote: ... 1 μL cDNA was amplified using 1 μL Phusion High-Fidelity DNA Polymerase (NEB) in HF Phusion buffer with 10 μM primers ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μL Bacillus stearothermophilus (Bst) DNA polymerase (8 U μl-1) (New England Biolabs), 2 μL DNA template ...
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bioRxiv - Molecular Biology 2020Quote: ... amplicons were visualized on ~1% agarose gels with 1 kb plus DNA ladder (NEB) and SYBR Gold (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2023Quote: ... containing 1× Exonuclease I Buffer and 1 U/µL Exonuclease I (NEB, cat # B0293S), and incubated at 37 °C for 45 min ...
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bioRxiv - Microbiology 2023Quote: ... and 1 μl of High Concentration T4 RNA Ligase 1 (New England BioLabs, M0437)] at 23°C for 5 h ...
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bioRxiv - Microbiology 2023Quote: ... lysates (1 mg) were incubated with MNase (10 units/1 mg lysate; NEB #M0247S) at 37°C for 60 min ...
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bioRxiv - Microbiology 2024Quote: ... the product was incubated with 1 μl of dTTP (1 mM, NEB Catalog # N443S), 1 μL of Tdt buffer ...
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bioRxiv - Microbiology 2024Quote: ... the product was incubated with 1 μL of dTTP (1 mM, NEB Catalog # N)443S) ...
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bioRxiv - Neuroscience 2022Quote: ... at −80 °C prior to RNA extraction and library preparation (performed by Novogene Company Limited with NEB Next® Ultra™ RNA Library Prep Kit (NEB)) prior to RNA sequencing (paired-end 150 bp ...
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bioRxiv - Neuroscience 2023Quote: ... at -80 °C prior to RNA extraction and library preparation (performed by Novogene Company Limited with NEB Next® Ultra™ RNA Library Prep Kit (NEB)) prior to RNA sequencing (paired-end 150 bp ...
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bioRxiv - Molecular Biology 2023Quote: ... from 4 to 15 hours at 37 °C. For Circle-Seq of the CRISPR treatments and controls (Fig. 5) exonuclease T (New England Biolabs; 20 units per μg of DNA) was then added ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1× CutSmart buffer (NEB) to 17 μl ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 x PNK buffer (NEB), and 20 U RiboLock ...
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bioRxiv - Genomics 2020Quote: ... 1 μl Exonuclease III (NEB), and NEBuffer 1 (NEB ...
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bioRxiv - Genomics 2019Quote: ... 1 ul Phi29 polymerase (NEB), 34 ul ultrapure water) ...
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bioRxiv - Genomics 2020Quote: ... RNase Inhibitor (1:40, NEB Murine RNAse Inhibitor or Thermofisher Ribolock ...
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Genetic mapping of fitness determinants across the malaria parasite Plasmodium falciparum life cyclebioRxiv - Evolutionary Biology 2019Quote: ... 1× BSA (New England Biolabs), 1 mM dNTPs (New England Biolabs) ...
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bioRxiv - Bioengineering 2019Quote: ... 1 × Zinc (supplied by NEB), 5 μL of 10 × GlycoBuffer 4 (supplied by NEB ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 μl Klenow exo-(NEB) and varying amounts of α[32P]-dCTP added before incubation for 1-3 hours at 37 °C ...
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bioRxiv - Synthetic Biology 2019Quote: ... 1× Taq polymerase buffer (NEB) and 2 μM or 20 μM each of the mutagenic nucleotide analogs 8-oxo-2′-deoxyguanosine-5′-triphosphate (8-oxo-dGTP ...
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bioRxiv - Genomics 2020Quote: ... 1 µL T4 PNK (NEB) and 6 µL H2O were mixed and incubated in a thermocycler (BioRad ...
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bioRxiv - Genetics 2019Quote: ... 1× T3 ligation buffer (NEB) and 300 ng total RNA with or without FTO treatment ...
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bioRxiv - Genomics 2021Quote: ... 1× BSA (New England Biolabs), 1 mM dNTPs (New England Biolabs) ...
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bioRxiv - Genomics 2021Quote: ... 1 μl of DpnI (NEB) was added to the reaction mixture to further incubate at 37 degrees for 10min ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and 1×Buffer 4 (NEB). The reaction was stopped (6 mM EGTA ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 1.875 U XRN-1 (NEB), 1 mM CaCl2 ...
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bioRxiv - Genomics 2020Quote: ... 1 µl RppH (5U, NEB)] was added ...
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bioRxiv - Genetics 2021Quote: ... 1 µl of T7EI (NEB) was then added to 19 µl of purified (QIAquick PCR Purification Kit ...
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bioRxiv - Genomics 2021Quote: ... 1 μL TET2 (NEB #7120S) and 1 μL T4-BGT (NEB #M0357S ...
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bioRxiv - Systems Biology 2020Quote: ... 1 μL T4 ligase (NEB), 2 μL T4 ligase buffer (NEB) ...
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bioRxiv - Biochemistry 2021Quote: ... and 1 mM ATP (NEB) in 1x PNK reaction buffer (70 mM Tris-HCl pH 7.6 ...
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bioRxiv - Cancer Biology 2020Quote: ... T7 Endonuclease 1 (NEB, M0302) was added to the mixture and incubated at 37°C for 15 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... 1 μl NAD+ (NEB, B9007) and 1 μl dNTP (Promega ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1 U/μl Esp3I (NEB), 0.5 U/μl T4 Polynucleotide Kinase (NEB) ...
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bioRxiv - Systems Biology 2020Quote: ... 1 U RNase Inhibitor (NEB)) ...
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bioRxiv - Microbiology 2020Quote: ... 1 μl T4 ligase (NEB) and 1 μl of the relevant restriction enzyme in a total volume of 20 μl made up in dH2O ...