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Citations for New England Biolabs :
2251 - 2300 of 5902 citations for 5 Nitro 1H benzo de isoquinoline 1 3 2H dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... CX plasmid (Bukhari et al., 2016) and 5′UTR containing amplicon was digested with NheI (NEB) followed by ligation with T4DNA ligase (NEB) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... supplemented with 10 units of Exonuclease we and 5 units of Lambda Exonuclease (New England BioLabs), was added to each sample ...
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bioRxiv - Molecular Biology 2023Quote: ... Library was PCR amplified for 5 cycles using the NEBNext High-Fidelity MasterMix (New England BioLabs) followed by qPCR amplification to determine the exact number of additional cycles required for optimal library amplification ...
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bioRxiv - Biochemistry 2023Quote: ... The HBB 5’ UTR containing mRNA was then capped using Vaccinia Capping enzyme (New England Biolabs) and 2′O-methylated using Vaccinia 2′O Methyltransferase (New England Biolabs) ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA (5 µg in 20 µL ddH2O) was incubated with 2µL 100U Nuclease P1 (NEB) and 2 µL 10x Nuclease P1 buffer (NEB ...
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bioRxiv - Biophysics 2024Quote: ... R4: 5’-TAGGTACAGTCCTCAGGTGTCTT[AmC6dT]T) were coupled with SNAP-substrate NHS ester (BG-GLA-NHS, NEB). The DNA strands at 1 mM concentration were incubated with 10 mM of BG-GLA-NHS and reactions were cleaned up using a Bio-Spin P-30 column (Bio-rad).
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bioRxiv - Microbiology 2024Quote: ... or INRKKR cRNA/mRNA (5’-CATGATTGTGTCAACCTGCTCTG) were radioactively labelled using T4 polynucleotide kinase (New England Biolabs) and ɣ-32P-ATP (PerkinElmer) ...
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bioRxiv - Immunology 2024Quote: ... 100 ng of gDNA was directly digested with 5 U of EcoRI-HF (New England Biolabs) in the ddPCR Supermix for Probes (no dUTPs ...
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bioRxiv - Cell Biology 2024Quote: ... Background assembly was measured by transformation of NEB 5-alpha competent cells (New England Biolabs C2987I) before cleanup of the assembly reactions using AmpureXP magnetic beads (Beckman Coulter A63880) ...
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bioRxiv - Biophysics 2023Quote: ... Topoisomerase I treated DNA was purified using Monarch PCR & DNA Cleanup Kit (5 μg) (NEB, T1030S) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Oligos were 5’-end radiolabeled at a final concentration of 4 μM with T4 PNK (NEB) and γ-32P-ATP after incubation for one hour at 37°C followed by enzyme inactivation at 72°C for 10 minutes ...
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bioRxiv - Genomics 2022Quote: ... a 5’ adapter was ligated onto the RNA transcript using T4 RNA Ligase I (NEB M0204L), and a third bead binding was performed ...
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bioRxiv - Microbiology 2022Quote: ... 5 μl of the cDNA was mixed with LongAmp Taq 2X Master Mix (New England Biolabs) and 100 pmol Sol-PrimerB (5’-GTTTCCCACTGGAGGATA-3’ ...
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bioRxiv - Genetics 2023Quote: ... All probes were 5’ end-labeled with [alpha-32P]-ATP and T4 polynucleotide kinase (NEB Inc.). The gels were exposed to a PhosphorImager ...
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bioRxiv - Molecular Biology 2023Quote: ... we treated the RNase I RPFs with 5 U of T4 PNK (NEB, 10 U/µL) after RNA size selection [19] ...
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bioRxiv - Microbiology 2024Quote: ... was conducted after phosphorylating the 5’ end of the pooled fragments with T4 PNK (NEB, #M0201S). Reverse transcription and PCR amplification of the library were followed by PAGE-assisted size selection were carried out as previously outlined67 ...
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bioRxiv - Molecular Biology 2024Quote: ... The R1R DNA adapter was adenylated by using a 5’ DNA Adenylation kit (New England Biolabs) and then ligated to the 3’ end of the cDNA by using Thermostable 5’ App DNA/RNA Ligase (New England Biolabs ...
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bioRxiv - Developmental Biology 2024Quote: ... Each qPCR reaction mixture contained 5 µL 2x Luna Universal qPCR master mix (New England BioLabs), 1 µL cDNA (2-fold dilution) ...
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bioRxiv - Immunology 2024Quote: ... the DNA was transformed into NEB® 5-alpha F’ I q chemically competent cells (NEB). Recombinants were selected on LB agar supplemented with 10 µg/ml of gentamicin ...
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bioRxiv - Biophysics 2024Quote: ... in T4 polynucleotide kinase buffer (NEB, 70 mM Tris-HCl, 10 mM MgCl2, 5 mM DTT) in a total reaction volume of 10 µL ...
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bioRxiv - Microbiology 2024Quote: ... All primers were 5’-phosphorylated with T4 polynucleotide kinase using an ATP-containing reaction buffer (NEB). Template backbone DNA was digested with DpnI (NEB) ...
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bioRxiv - Synthetic Biology 2024Quote: ... The singly modified 13nt-RNA was 5’ phosphorylated using T4 PNK (10U/µl New England Biolabs) according to the protocol provided by the manufacturer ...
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bioRxiv - Genomics 2024Quote: ... 5 ng of cDNA were amplified with LongAmp Taq 2X Master Mix (M0287S, New England Biolabs) for 25 cycles ...
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bioRxiv - Molecular Biology 2024Quote: 10 µg of DNase-treated total RNA was digested with 5 U of RNase H (NEB) and 2 picomoles of a chimeric oligonucleotide targeting the MSRs for 20 minutes at 37 ℃ ...
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bioRxiv - Molecular Biology 2024Quote: ... Amplification of target sequences under 5 kb were produced using Q5 high fidelity polymerase (NEB # M0491S) under the recommended three-step amplification conditions ...
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bioRxiv - Molecular Biology 2024Quote: ... Cloning strains used were NEB Stable (lentiviral) and NEB 5-alpha (other plasmids) (New England Biolabs). For base editor cloning ...
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bioRxiv - Plant Biology 2024Quote: ... Approximately 5 μg of mRNA mixed with 10 fmol of methylated and unmethylated standards (NEB, E1610S) was used for fragmentation (NEB ...
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bioRxiv - Bioengineering 2024Quote: ... 5 μl of clean DNA was transformed into 50 μl of DH5α commercial competent cells (NEB). The whole transformation was spread onto a 10 cm ampicillin agar plate ...
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bioRxiv - Plant Biology 2024Quote: ... For molecular cloning and plasmid propagation Escherichia coli strain NEB® 5-alpha (New England Biolabs) and Stellar™ Competent Cells (Takara Bio Group ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by adding 5 mM MgCl2 and 0.5 units of shrimp alkaline phosphatase (NEB, cat # M0371S) at 37°C for 15 minutes ...
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bioRxiv - Cancer Biology 2024Quote: Genomic DNA was extracted from 5×106 cells using a Monarch Genomic DNA extraction kit (NEB). 2 ng of genomic DNA was incubated at 55°C for 15 minutes with 0.4 ul TDE1 (Illumina ...
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bioRxiv - Cell Biology 2024Quote: ... MLX and HK2 were incubated with 5 µL of CIP (Quick CIP New England Biolabs, M0525S) and/or PhosSTOP (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... with 5 μL Quick-Load pBR322 DNA-MspI Molecular Marker (E7560S, New England Biolabs, MA, USA) run in a separate lane ...
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bioRxiv - Bioengineering 2024Quote: ... cells were incubated on ice for 5 minutes and mixed with 450 µl SOC-medium (NEB). Transformed cells were then plated on agar plates containing the respective antibiotics according to the supplier’s information ...
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bioRxiv - Bioengineering 2024Quote: ... Intramolecular circularization was performed at a concentration of 5 ng/µL with T4 DNA ligase (NEB), and treated with a cocktail of exonucleases ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries were prepared by amplifying DNA for 5 cycles with NEBNext 2x MasterMix (New England Biolabs). After performing qPCR to determine additional cycles needed ...
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bioRxiv - Microbiology 2022Quote: The recombinant constructs (pETDuet-1-TaPHB-1 and pETDuet-1-TaPHB-1-RUVBL1 were separately transformed into E. coli Lemo21(DE3) (NEB) chemical competent cells ...
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bioRxiv - Molecular Biology 2023Quote: ... Unincorporated ddRTP molecules were inactivated by adding 1 µL of Buffer 2 (80 mM MgCl2) and 1 µL of 1:1 rSAP (NEB):50% glycerol ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 μL T4 RNA Ligase 1 (NEB, M0437M), 1 μL SuperAseIn (Invitrogen AM2694) ...
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bioRxiv - Genomics 2021Quote: ... and washed 3 times with ice-cold 10 mM Ribonucleoside Vanadyl Complex (RVC) (New England BioLabs, cat.no. S1402S, Ipswich, Mass, USA) in buffer A (10 mM NaCl ...
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bioRxiv - Developmental Biology 2021Quote: ... The calibrated small RNA samples were ligated to an adenylated and fluorescently labeled 3’ adaptor using T4 RNA ligase 2 truncated KQ (NEB, M0373S) and were subsequently separated on a 12% polyacrylamide UREA gel ...
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bioRxiv - Developmental Biology 2021Quote: ... 3’UTRs were combined to the TagRFP-T CDS using the Gibson assembly Master Mix (Cat#E2611, New England BioLabs Inc). The resulting fragment was then amplified via PCR and digested prior ligation into a vector containing only Hofstenia promoter region ...
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bioRxiv - Neuroscience 2021Quote: ... The plasmid was linearized with SacI or XhoI (for transcription from T7 or SP6 promoter respectively) and 3’UTR fragment was transcribed in vitro using SP6 or T7 polymerases (New England Biolabs, UK) and the DIG RNA labelling Mix (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNA probes were complimentary to the 3′-UTR regions immediately adjacent to the poly(A) tails and were labeled with T4 PNK (NEB, M0201S) and [γ-32P]ATP (PerkinElmer) ...
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bioRxiv - Cell Biology 2022Quote: ... 200 pmol of an equimolar mixture of all gene-specific oligos for each gene were mixed with 250 pmol of the appropriate FLAP oligo in 1x NEBuffer 3 (New England Biolabs, B7003), then incubated in a Thermocycler (BioRad ...
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bioRxiv - Microbiology 2021Quote: ... the SAG1 3’UTR was amplified from pNJ-26 and cloned into the tagging plasmid to replace DHFR 3’UTR by Gibson assembly (NEB, E5520S). BAG1-mCherry GCaMP6f reporter tachyzoites were co-transfected with 10 μg of pSAG1::CAS9-U6::sgDHFR 3’UTR and 2 μg of PCR amplified P2A-mTagBFP2-HXGPRT flanked with 40 bp homology regions ...
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bioRxiv - Neuroscience 2020Quote: Overlapping fragments of the unstable NaV1.1 cassette-3 were amplified in polymerase chain reactions (PCR) using Q5® Hot Start High Fidelity 2x Master mix (New England Biolabs) and the primer pairs listed in Table S1 ...
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bioRxiv - Neuroscience 2020Quote: ... 3’ homozygous arm was amplified from y,sc,v genomic DNA using Q5 High-Fidelity DNA Polymerase (New England BioLabs, NEB) with primers ...
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bioRxiv - Microbiology 2020Quote: ... All genomic insertions were targeted to the 3’ end of the glmS gene of ICC8001 and all constructs were generated by Gibson Assembly (New England Biolabs, US).
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bioRxiv - Neuroscience 2020Quote: ... 3 DNA fragments were generated with PCR from genomic DNA or plasmids using Q5 High-Fidelity DNA Polymerase (NEB Cat# M0491). Fragment 1 included the genomic DNA between guide 1 and the KI site plus flipped guide 2 (with PAM ...