Labshake search
Citations for New England Biolabs :
2201 - 2250 of 9545 citations for RPA kit reagent since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... DHFR control plasmid from PURExpress In Vitro Protein Synthesis Kit (New England Biolabs) was used as backbone for subsequent ligations ...
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bioRxiv - Genomics 2022Quote: ... The resulting fragments were purified using the Monarch PCR & DNA Cleanup Kit (NEB). Libraries were constructed using Illumina Nextera Dual Indexing ...
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bioRxiv - Genomics 2022Quote: ... the original ORI was excised using a Site Directed Mutagenesis kit (NEB E0554S). The vector was transformed (NEB C3040H ...
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bioRxiv - Genomics 2023Quote: ... Sequencing libraries was constructed using NEBnext Ultra II DNA library prep kit (NEB) following the manufacture’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and mutations were introduced using the Q5 Site-Directed Mutagenesis Kit (NEB # E0554S) or were synthesized by Genscript ...
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bioRxiv - Molecular Biology 2023Quote: ... dsRNA was generated by in vitro transcription using the HiScribe T7 kit (NEB), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Secondary PCRs were purified with the Monarch PCR and DNA Cleanup Kit (NEB), ligated into the pCR2.1-TOPO TA cloning vector (Invitrogen) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... double strand cDNA was synthesized using NEBNext mRNA second strand synthesis kit (NEB), double strand DNA was washed with AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Plant Biology 2023Quote: ... Library was prepared using the NEBNext Ultra Directional RNA Library Prep Kit (NEB) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... rRNA depletion was conducted using NEBNext rRNA Depletion Kit (Bacteria, New England Biolabs) according to the manufacturer’s recommended protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA was purified by Monarch® RNA Cleanup Kit (New England Biolabs). Binding of ribosomes to leaderless mRNA was tested by assembling 10-500 fmol of 70S ribosomes with 10 fmol of NAD/ ppp-leaderless mRNA in translation buffer (10 mM Tris-HCl pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... and the NEBNext Ultra II directional RNA library Prep kit (NEB, Cat#E7785S) for Illumina were used for index library prep following NEB #E7760S/L ...
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bioRxiv - Bioengineering 2023Quote: The Luna Universal Probe One-Step RT-qPCR kit (New England Biolabs, USA) was used ...
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bioRxiv - Bioengineering 2023Quote: ... and the linearized plasmid was re-ligated with a Quick Ligase Kit (NEB) following manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The linearized DNAs were purified using Monarch DNA cleanup kit (NEB, United States) and the capped RNA transcripts were synthesized using the SP6 mMessage mMachine kit (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... Sequencing libraries were prepared using NEB Next Ultra II kit (New England Biolabs). The resulting DNA quantity and size distribution was assessed by Qubit instrument (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... and NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB; Ipswich, USA). RNA-Seq was then performed at a depth of 50M reads/sample on an Illumina NovaSeq 6000 S4 instrument of 100 base-pair long paired-end sequence reads (excluding adapter/index sequences) ...
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bioRxiv - Genomics 2023Quote: ... rRNA depletion was performed using the NEBNext® rRNA Depletion Kit (Bacteria) (NEB) and the sequencing libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit (NEB ...
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bioRxiv - Neuroscience 2023Quote: ... NEBNext Ultra II RNA Library Preparation Kit for Illumina (#E7770; New England Biolabs) was used ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The repair template plasmid (pKK4) was assembled using the NEBuild HiFi Kit (NEB) from a combination of a restriction digest fragment and a synthetic gBlock Gene Fragment (IDT) ...
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bioRxiv - Synthetic Biology 2023Quote: ... for large rearrangements and Q5® Site-Directed Mutagenesis Kit (New England BioLabs) for small modifications i ...
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bioRxiv - Plant Biology 2023Quote: ... Reactions were purified using a Monarch PCR & DNA Cleanup Kit (New England Biolabs) and sent to Source BioScience ...
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Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteinsbioRxiv - Bioengineering 2023Quote: ... site-directed mutagenesis was conducted using the Q5 Site-Directed Mutagenesis Kit (NEB). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... FN2 deletion was accomplished using Q5 Site-Directed Mutagenesis kit (New England Biolabs) according to the manufacturer’s directions and in-frame deletion was verified with Sanger sequencing ...
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bioRxiv - Cancer Biology 2024Quote: ... was conducted using the Monarch® Genomic DNA Purification Kit (NEB, Cat # T3010S). Primers were procured from Integrated DNA Technologies and designed with the PrimerQuest™ Tool (IDT ...
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bioRxiv - Cancer Biology 2024Quote: ... rRNA was depleted from total RNA using the rRNA depletion kit (NEB #E6310). After fragmentation of the rRNA reduced RNA ...
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bioRxiv - Systems Biology 2024Quote: ... pp65 mutants were generated using Q5 site-directed mutagenesis kit (New England Biolabs).
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bioRxiv - Systems Biology 2024Quote: ... The digests were then purified using a Monarch PCR&DNA Cleanup Kit (NEB) and amplified using firstly a nested PCR reaction with KAPA HiFi HotStart ReadyMix (Roche) ...
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bioRxiv - Synthetic Biology 2024Quote: ... the RNA was purified with the Monarch RNA Cleanup Kit (NEB, Cat. #T2050) and run on a 1.5% agarose gel with 0.3% hydrogen peroxide (Supplementary Figure S7a)45 ...
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bioRxiv - Systems Biology 2024Quote: ... Libraries were prepared using NEBNext Ultra Directional RNA Library Prep Kit (NEB, E7420) and PCR amplified (15 min at 37°C ...
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bioRxiv - Cell Biology 2024Quote: crRNAs were synthesized using HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... coli overnight cultures using a Monarch plasmid miniprep kit (New England Biolabs, USA) and concentration was quantified by NanoDrop (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... IVT product was then reverse transcribed with a ProtoScript II kit (NEB, E6560S) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... In vitro transcription was performed using a T7 Quick HiScribe kit (NEB, E2050S) according to manufacturer’s instructions with overnight incubation at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Editing activity of α-synCas was evaluated using EnGen Mutation Detection Kit (NEB). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... Double-stranded RNA was purified using Monarch RNA Cleanup Kit (50 µg; NEB) and eluted in 30 μl of nuclease-free water ...
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bioRxiv - Biochemistry 2023Quote: ... Site-directed mutagenesis was performed using the Q5 site-directed mutagenesis kit (NEB).
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bioRxiv - Biochemistry 2023Quote: ... and the NEBNext Ultra RNA library prep kit (New England Biolabs, MA, U.S.A) manufacture’s protocol followed to prepare the sample for Illumina® RNA-sequencing (RNA-seq) ...
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bioRxiv - Biochemistry 2023Quote: ... GST-tag HP1⍺ΔC construct was generated by site-direct mutagenesis kit (NEB) by deleting the amino acids (number 169 –181 ...
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Measuring carbohydrate recognition profile of lectins on live cells using liquid glycan array (LiGA)bioRxiv - Biochemistry 2023Quote: ... PCR fragments were processed using the NEBuilder Hifi DNA assembly kit (NEB #E2621S) using the steps described above.
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bioRxiv - Cancer Biology 2023Quote: ... adaptors were ligated to the purified amplicons using the Quick Ligation Kit (NEB), and the ligation products were purified using the Agencourt AMPure XP system (Beckman Coulter) ...
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A CRISPR activation screen identifies FBXO22 as an E3 ligase supporting targeted protein degradationbioRxiv - Biochemistry 2023Quote: ... FBXO22 mutants were generated using Q5 site-directed mutagenesis kit (New England Biolabs) following the manufacturer’s instruction.
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bioRxiv - Cell Biology 2023Quote: ... The NEBNext Ultra II RNA Library Prep Kit for Illumina® (NEB, #E7775) was then used to prepare libraries for paired-end (2×150 bp ...
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Emergence of RNA-guided transcription factors via domestication of transposon-encoded TnpB nucleasesbioRxiv - Genetics 2023Quote: ... RNA was extracted using TRIzol and column purification (NEB Monarch RNA cleanup kit), and samples were then individually diluted in NEBuffer 2 (NEB ...
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bioRxiv - Genetics 2023Quote: ... and libraries prepared using NEB Ultra II DNA Library Prep Kit (NEB, #E7645S) and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs Set 4 ...
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bioRxiv - Cell Biology 2023Quote: ... and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB 7760) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: Plasmid DNA was purified using the Monarch Plasmid DNA Miniprep Kit (NEB, #T1010), and subjected to diagnostic restriction digest and subsequent gel electrophoresis ...
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bioRxiv - Microbiology 2023Quote: ... The resulting DNA fragments were cloned using the Gibson Assembly Cloning Kit (NEB) into plasmid backbone pUN4 to obtain pUN-OXA48WT ...
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bioRxiv - Plant Biology 2023Quote: ... followed by purification with the Monarch RNA Clean Up Kit (NEB, Frankfurt, Germany). gDNA removal was assessed by PCR on chloroplast DNA followed by agarose gel electrophoresis ...
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bioRxiv - Plant Biology 2023Quote: ... ribosome footprints were purified using the Monarch RNA cleanup kit (NEB cat# T2030) following the modified protocol for small RNA.