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Citations for New England Biolabs :
2051 - 2100 of 9545 citations for RPA kit reagent since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... Samples were then purified and concentrated using a Monarch RNA Cleanup Kit (NEB). RNA samples were quantified and sent to BGI (China ...
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bioRxiv - Microbiology 2022Quote: ... Using the Q5® High-Fidelity DNA Polymerase PCR Kit (New England BioLabs) for SA11 VP4 and Platinum™ Taq DNA polymerase (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... Fragmented DNA was repaired using NEBNext blunt-end repair kit (New England Biolabs) and purified using Monarch PCR clean-up kit (NEB) ...
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bioRxiv - Microbiology 2022Quote: ... Reverse-transcription PCR was then performed using LunaScript RT Master Mix Kit (NEB) using a gene specific primer ...
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bioRxiv - Microbiology 2022Quote: ... NEBNext Ultra II RNA Library Prep Kit (Catalog: Catalog: E7775S, New England Biolabs) for Illumina was used for the library preparation ...
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bioRxiv - Microbiology 2022Quote: ... After electrophoresis and gel extraction by Monarch DNA Gel Extraction Kit (T1020S; NEB), the PCR product was transcribed using HiScribe T7 High Yield RNA Synthesis Kit (E2040S ...
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bioRxiv - Microbiology 2022Quote: ... the amplicons were purified with the Monarch® PCR & DNA Cleanup Kit (NEB). High-throughput sequencing was performed by Novogene using the Illumina NovaSeq PE250 system (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... and the remainder was cloned by using PCR Cloning Kit (New England Biolabs) and analyzed by Sanger sequencing.
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bioRxiv - Microbiology 2022Quote: ... and the sequences were introduced with the Q5 site-directed mutagenesis kit (NEB). Transfection of both the TATi cassette and the Cas9/guide RNA vector was performed as above ...
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bioRxiv - Microbiology 2022Quote: ... and DNase was removed by using the Monarch RNA Cleanup Kit (50μg; NEB). LunaScript RT SuperMix Kit (NEB ...
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bioRxiv - Microbiology 2022Quote: ... we used the Monarch HMW DNA Extraction Kit for Tissue (NEB, MA, USA) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... cell DNA was extracted using the Monarch genomic DNA purification kit (NEB #T3010L) or the NucleoSpin Tissue kit (Macherey-Nagel #740952-250 ...
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bioRxiv - Microbiology 2022Quote: ... Ribosomal RNA depletion was performed with the NEBNext Bacteria kit (NEB, Frankfurt, Germany). The libraries were prepared with the TruSeq kit (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... and the RNA was purified with Monarch® RNA clean-up kit (NEB). sgRNA aliquots were stored at −80°C.
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bioRxiv - Molecular Biology 2022Quote: ... Mutations were made with Q5® Site-Directed Mutagenesis Kit (New England Biolabs) using primer sets (Supplementary file 1) ...
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bioRxiv - Plant Biology 2022Quote: ... sequencing libraries were generated using Directional NEBNext Ultra RNA Library Prep Kit (NEB) (Novogene ...
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bioRxiv - Plant Biology 2022Quote: ... Point mutations were generated using a Q5 mutagenesis kit (New England Biolabs, USA). Plasmids were amplified in DH5α E ...
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bioRxiv - Molecular Biology 2023Quote: ... and NEBNext Ultra II Directional RNA Library Prep Kit (New England BioLabs, #E7765) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... purified PCR products were cloned using NEB PCR Cloning Kit (New England BioLabs). Clones were Sanger sequenced at the University of Minnesota Genomics Center.
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bioRxiv - Molecular Biology 2023Quote: ... Human FBP1 mutants were constructed by Q5 Site-Directed Mutagenesis Kit (NEB, E0554S) in the PCDH-V5-FBP1 vectors ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing libraries were made using the Small RNA library preparation kit NEBNext (NEB). Sequencing was performed on the Illumina HiSeq 2500 using the Rapid Run mode.
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bioRxiv - Microbiology 2023Quote: ... and tasA_downstream_rv and the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs).
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bioRxiv - Microbiology 2023Quote: ... and was further prepared for sequencing using NEBNext Ultra FS II kit (NEB) with the miniaturized protocol (22) ...
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bioRxiv - Microbiology 2022Quote: All enzymes and DNA preparation kits used were from New England Biolabs (NEB), and all PCR products were amplified using Phusion polymerase ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was synthesized with Protoscript First Strand Synthesis Kit (New England Biolabs #E6300L) and pre-amplified for 14 cycles against a pool of primers (Supplemental Table 3 ...
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bioRxiv - Synthetic Biology 2022Quote: ... The PCR product was purified using Monarch® PCR & DNA Cleanup kit (NEB) and eluted in 10 μL of Ultra Pure™ DNase/RNase free distilled water ...
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bioRxiv - Synthetic Biology 2022Quote: ... The PCR product was purified using Monarch® PCR & DNA Cleanup kit (NEB) and eluted in 10 μL of Ultra Pure™ DNase/RNase free distilled water ...
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bioRxiv - Physiology 2022Quote: ... using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and considering three biological replicates for each plant material ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCR amplifications were column-purified using the Monarch PCR & DNA Cleanup Kit (NEB), and assembled using NEBuilder HiFi DNA Assembly Master Mix (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... IVT mRNA was purified using a Monarch RNA Cleanup Kit (New England Biolabs). IVT mRNA was then transfected into cells using Lipofectamine MessengerMAX (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by purification with Monarch PCR & DNA Cleanup Kit (T1030, New England Biolabs). The end-repaired cDNA was ligated with 2 μL barcoded adaptor (100-466-000 ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by purification with Monarch PCR & DNA Cleanup Kit (T1030, New England Biolabs). The un-ligated adaptor and cDNA were digested with E ...
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bioRxiv - Molecular Biology 2022Quote: ... and purified with the Monarch PCR & DNA Cleanup Kit (T1030, New England Biolabs).
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bioRxiv - Microbiology 2022Quote: ... cDNA was synthesized using First-strand cDNA synthesis kit (New England BioLabs, Inc.) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... The pre-crRNA was purified using Monarch RNA Cleanup Kit (NEW ENGLAND BioLabs). RNAs were resuspended in diethyl pyrocarbonate H2O and stored at −80°C.
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bioRxiv - Cell Biology 2022Quote: ... ORF6 mutants were generated by using Q5 Site-Directed Mutagenesis Kit (NEB, #E0554S) or QuikChange II XL Site-Directed Mutagenesis Kit (Agilent ...
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bioRxiv - Biochemistry 2022Quote: ... and Gibson assembly with NEBuilder HiFi DNA Assembly Cloning kit (New England Biolabs) into pFastBac vector (Thermo Fisher ...
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bioRxiv - Molecular Biology 2022Quote: ... and then reverse transcribed using ProtoScript II first-strand cDNA synthesis kit (NEB) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... followed by ribosomal RNA (rRNA) depletion using NEBNext rRNA Depletion Kit (E6310, NEB). The ribosomal-depleted RNA was used to make the RNA-seq libraries using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (E7760 ...
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bioRxiv - Plant Biology 2022Quote: ... and NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). Sequencing was performed using NovaSeq 6000 (Illumina ...
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bioRxiv - Neuroscience 2022Quote: ... Single guide RNA (sgRNA) was synthesized using EnGen sgRNA Synthesis Kit (NEB, E3322) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: cDNA was prepared using LunaScript® RT SuperMix Kit (New England Biolabs, #E3010L), together with no-RT control reaction and no template controls ...
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bioRxiv - Cell Biology 2022Quote: ... Sequencing libraries were prepared using the NEBNext® Ultra II kit (NEB, E7645S) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA was then purified with Monarch PCR & DNA Cleanup Kit (New England BioLabs). Libraries were prepared using Q5 High-Fidelity 2X Master Mix NEB ...
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bioRxiv - Developmental Biology 2024Quote: ... Amplified libraries were purified with Monarch PCR & DNA Cleanup Kit (New England BioLabs) and sequences Illumina NextSeq 2000 system at Boston University Microarray and Sequencing core ...
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bioRxiv - Developmental Biology 2024Quote: ... The Tg(UAS:GFP-P2A-ppp3ccacA) has been generated by ligation (KLD kit, NEB) of PCR fragments GFP-P2A-ppp3ccacA (IM459/IM460 ...
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bioRxiv - Biochemistry 2024Quote: ... Fragments were ligated together with NEBuilder HiFi DNA Assembly Kit (New England Biolabs). Then Escherichia coli DH5α competent cells were transformed with the ligation mixtures and the cells containing assembled plasmid were selected on an agar medium supplemented with 100 mg/l ampicillin ...
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bioRxiv - Bioengineering 2024Quote: ... The mRNA was purified using the Monarch RNA Cleanup Kit (NEB, MA, UK). Followed reagents were used for IL-15 superagonist characterization ...
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bioRxiv - Bioengineering 2024Quote: ... Transcription reactions were purified using the Monarch RNA cleanup kit (New England Biolabs). mRNA concentration was quantified by nanodrop spectrophotometer ...
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bioRxiv - Bioengineering 2024Quote: ... Synthesized mRNA was purified using the Monarch RNA cleanup kit (New England Biolabs) and stored at −80°C ...