Labshake search
Citations for New England Biolabs :
2201 - 2250 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: All purified SNAP-tagged proteins were labeled with 10-fold molar excess of SNAP-Surface dyes (New England Biolabs, Ipswich, MA) in labeling buffer (1× PBS ...
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bioRxiv - Microbiology 2023Quote: ... Each protein was then isolated and affinity-purified using an amylose resin according to the manufactureŕs protocol (New England Biolabs, Ipswich, US) and the concentration was determined using the Bradford technique ...
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CXCL17 binds efficaciously to glycosaminoglycans with the potential to modulate chemokine signallingbioRxiv - Immunology 2023Quote: ... WT SUMO3-CXCL17 and variants were expressed as soluble proteins in SHuffle® T7 Competent E.coli (New England Biolabs, Hitchin, UK). In both protocols ...
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bioRxiv - Cell Biology 2023Quote: ... The protein was then purified by affinity-purification using an amylose beads according to the manufacturer’s protocol (New England Biolabs, Ipswich, USA) and the concentration determined by Bradford assay ...
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bioRxiv - Cell Biology 2023Quote: Beads with bound Flag-RAD50 or GFP-BRCA1 were washed with 5M NaCl to eliminate contamination with protein kinases and incubated with 100 or 500 U of CK2 holoenzyme complex (α2/β; NEB) in 30 μl of kinase buffer (20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Molecular Biology 2023Quote: ... Unligated 3’ linker was removed by incubating the samples with the 5’-deadenylase KmHnt3 (see Recombinant protein expression and purification) and RecJ exonuclease (New England Biolabs, M0264S) for 45 min at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: The cell lysates or purified PINK1 bound to FLAG beads were incubated at 30°C for 1h with 200 units of ʎ protein phosphatase (LPP) in a total reaction of 100µL following the instructions of the manufacturer (New England Biolabs #P0753).
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bioRxiv - Cell Biology 2024Quote: We fused SNAP-3xHA coding sequences downstream H3.1 and H3.3 CDS 46 and inserted this fusion protein into the pBS31 vector 70 using NEBuilder® HiFi DNA Assembly Master Mix (NEB). These expression vectors (pB31-H3.1- and pB31-H3.3-SNAP-3xHA ...
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bioRxiv - Evolutionary Biology 2020Quote: Sequencing libraries were prepared using either the “NEBNext Ultra II DNA Library Prep Kit for Illumina®” (E7645) or the “NEBNext Ultra II FS DNA Library Prep Kit for Illumina®” (E7805) (New England BioLabs, Ipswich, USA). Sequencing was conducted on an Illumina MiSeq System (Illumina Inc. ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The described method follows the NEBNext® Ultra™ II DNA Library Prep Kit (v4.0) with Sample Purification Beads Kit (New England Biolabs, Ipswich, Massachusetts, USA). But other kits can be used too ...
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bioRxiv - Evolutionary Biology 2021Quote: ... or the NEBNext Ultra DNA Library Prep Kit for Illumina (formerly NEBNext DNA Library Prep Kit for Illumina; New England Biolabs Inc., Ipswich, Massachusetts, U.S.) and the NEBNext Multiplex Oligos for Illumina were used for library preparations ...
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bioRxiv - Developmental Biology 2019Quote: ... An amount of 500 ng total RNA was used for RNA-seq library preparation with NEB NEBNext rRNA Depletion Kit and ENBNext Ultra Directional RNA Library Prep Kit (New England Biolabs Japan Inc., Tokyo, Japan); 2 × 36 base paired-end sequencing was performed with NextSeq500 (Illumina K.K. ...
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bioRxiv - Neuroscience 2024Quote: ... Samples with a RIN greater than 7 were processed for library preparation with NEBnext rRNA depletion kit and ULTRAII FS RNA-seq Library Preparation Kit for Illumina (New England Biolabs, Ipswich, MA, United States). This procedure involved steps for mRNA enrichment ...
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bioRxiv - Microbiology 2020Quote: ... NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB), and NEBNext Multiplex Oligos for Illumina (NEB) ...
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bioRxiv - Cell Biology 2020Quote: ... using the Q5 site-directed mutagenesis kit (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... the Q5® site-directed mutagenesis kit (New England Biolabs) was used on the Patgl-1::GFP plasmid previously generated (Noble et al. ...
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bioRxiv - Cell Biology 2020Quote: ... or BioLux® Cypridina Luciferase Assay Kit (New England Biolabs) and Gaussia luciferase was assayed from 1:40 diluted cell culture supernatant using either the Stop & Glo reagent from the DualLuciferase® Reporter Assay System (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... or BioLux® Gaussia Luciferase Assay Kit (New England Biolabs) in a Lumat LB 9507 luminometer (Berthold Technologies).
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bioRxiv - Immunology 2021Quote: ... NEXTFLEX barcodes were ligated using the Quick Ligation Kit (NEB). Libraries were amplified for 15 cycles using HotStart HiFi Ready Mix (KAPA) ...
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bioRxiv - Immunology 2021Quote: ... and NEBnext Ultra II RNA library prep kit (NEB E7770S). Briefly ...
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bioRxiv - Genetics 2019Quote: ... mRNA was enriched using an NEBNext rRNA Depletion kit (NEB). Libraries for sequencing were prepped using an NEBNext Ultra II Library prep kit for Illumina (NEB ...
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bioRxiv - Genetics 2021Quote: ... Capped mRNA was synthesized using the HiScribe SP6 kit (NEB) and purified using the RNA Clean & Concentrator kit (Zymo) ...
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bioRxiv - Genetics 2021Quote: ... The NEBNext Library Quant Kit for Illumina (New England Biolabs) was used for the qPCR-based quantification of the pooled library ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and purified with the Monarch PCR & DNA Cleanup Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... NEBNext Ultra II RNA Library Prep kit (New England Biolabs) was used to perform poly-A selection and prepare libraries for sequencing ...
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bioRxiv - Immunology 2022Quote: ... and Luna® Universal RT-PCR Kit (New England Biolabs) in a StepOnePlus apparatus (Applied Biosystems ...
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bioRxiv - Neuroscience 2021Quote: NEBuilder® HiFi DNA Assembly cloning kit (NEB, Cat# E5520S) was used to clone HexB gene promoters of various sizes and species into a promoterless AAV plasmid vector containing gaussia dura luciferase and GFP (LG ...
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bioRxiv - Molecular Biology 2020Quote: Libraries were prepared using NEB Next kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... rRNA was depleted using the NEBNext rRNA Depletion kit (NEB). RNA-seq libraries were prepared using the NEBNext Ultra Directional RNA Library Prep kit (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid DNA was purified with mini-prep kits from NEB. Screening PCRs were performed using DreamTaq polymerase (Thermo Fisher) ...
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bioRxiv - Bioengineering 2022Quote: ... The PURExpress® in-vitro synthesis kit (New England Biolabs) was used for in-vitro protein synthesis ...
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bioRxiv - Genomics 2020Quote: ... RNA was purified using a Monarch RNA purification kit (NEB). RNA quality was evaluated with an Agilent Tape station ...
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bioRxiv - Microbiology 2019Quote: ... NEB One Taq One-Step RT-PCR Kit (NEB # E5310S) was used for nucleic acid amplification ...
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bioRxiv - Genomics 2020Quote: ... using the NEBNext Ultra II DNA Library Prep Kit (NEB). ChIP samples were single-end (1 × 75 bp ...
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bioRxiv - Developmental Biology 2019Quote: 2.3.4 The Q5 Site-Directed Mutagenesis Kit (NEB, Cat #: E0554S) can be used to make deletion and substitution mutations of DNA constructs.
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bioRxiv - Developmental Biology 2019Quote: ... purified with the Monarch PCR and DNA Cleanup kit (NEB), and then dialyzed with 0.5X TE buffer using a 0.025 μm VSWP membrane (Millipore ...
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bioRxiv - Immunology 2019Quote: ... purified using the Monarch Gel Extraction kit (New England Biolabs), and cleaned with MinElute PCR Purification Kit (Qiagen) ...
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bioRxiv - Molecular Biology 2020Quote: ... removing DNA overhangs with the Quick Blunting™ Kit (NEB), and circularization with T4 Ligase (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... MeRIP was performed using EpiMark N6-methyladenosine Enrichment kit (NEB) according to the manufacturer’s recommendations with the following modifications ...
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bioRxiv - Microbiology 2019Quote: ... using T7 RNA polymerase (HiScribe T7 kit, New England Biolabs) according to manufacturer’s instructions (E ...
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bioRxiv - Synthetic Biology 2019Quote: ... and assembled using NEBuilder HiFi DNA Assembly Cloning Kit (NEB). Plasmid preparations were carried out using QIAprep Spin Miniprep Kit (QIAGEN) ...
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bioRxiv - Cancer Biology 2020Quote: ... was bisulfite-converted by using the Epimark Methylation kit (NEB). Each experiment included non-CpG cytosines as internal controls to detect incomplete bisulfite conversion of the input DNA ...
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bioRxiv - Genetics 2019Quote: ... NEBNext® Ultra™ RNA Library Prep Kit (E7530L, NEB) was used to construct RNA-seq library from immunoprecipitated RNA and input RNA ...
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bioRxiv - Immunology 2020Quote: All new plasmids were assembled using NEBuilder kit (NEB # E5520S) following the manufacturer’s recommendations unless otherwise noted ...
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bioRxiv - Cell Biology 2019Quote: ... The HiScribe™ T7 ARCA mRNA kit (New England BioLabs) was used to transcribe PIWIL3-EGFP ...
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bioRxiv - Genomics 2019Quote: ... and ligation of indexed sequencing adaptors (Quick Ligation kit, NEB). We PCR amplified libraries to a final concentration with 10 cycles using KAPA HiFi HotStart ReadyMix and quantified them using bioanalyzer ...
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bioRxiv - Genetics 2019Quote: ... the Q5® Site-Directed Mutagenesis Kit (New England Biolabs) was used according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... Libraries were made with Ultra II DNA kits from NEB and sequenced on an Illumina miSeq and/or nextSeq.
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bioRxiv - Genomics 2020Quote: ... Samples were end-repaired with the Quick Blunting Kit (NEB) and purified with Agencourt AMPure XP (Beckman Coulter ...
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bioRxiv - Immunology 2021Quote: ... then isolated using a Monarch® Plasmid Miniprep Kit (NEB). The isolated products were then sequenced to ensure proper addition of the CAD gene sequence to the MigR1 backbone.