Labshake search
Citations for New England Biolabs :
2451 - 2500 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... sgRNAs were synthesized using the HiScribe T7 kit (NEB, E2050S) and purified by the RNA purification Kit (Zymogen ...
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bioRxiv - Genomics 2023Quote: ... and extracted their RNA using a miniprep kit (NEB, T2010S), performed reverse transcription (NEB ...
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bioRxiv - Genomics 2023Quote: ... NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) was used to prepare indexed sequencing libraries following the manufacturer supplied protocol ...
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bioRxiv - Immunology 2023Quote: ... Using the NEBridge® Golden Gate Assembly Kit (NEB, E1602), individual fragments were assembled to full-length RBD gene segments ...
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bioRxiv - Microbiology 2023Quote: ... and cDNA generated using LunaScript® RT Supermix kit (NEB). qPCR was performed using Luna® Universal qPCR Master Mix (NEB ...
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bioRxiv - Microbiology 2023Quote: ... Gel extraction and plasmid preparation kits were purchased from NEB and Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... RNA was processed using Monarch RNA Cleanup Kit (NEB T2030L) and quantified on a Nanodrop Spectrophotometer 2000 ...
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bioRxiv - Genomics 2023Quote: ... using the NEBNext Enzymatic Methyl-seq Kit (NEB, Cat # E7120L).
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bioRxiv - Microbiology 2023Quote: ... The NEBuilder® HiFi DNA Assembly Kit (New English Biolabs) was used to insert the following elements into the pUC18 plasmid ...
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bioRxiv - Developmental Biology 2023Quote: ... using the Q5 site directed mutagenesis kit (NEB, Ipswich, MA) and primers RA1318 and RA1063 ...
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bioRxiv - Cell Biology 2023Quote: ... The mRNA was purified by Monarch RNA Cleanup Kit (NEB). Purified mRNAs were stored at 2-6 μg/μl concentration and were diluted prior to injection with Femtojet to 100-500 ng/μl.
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bioRxiv - Cell Biology 2023Quote: ... using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB; E2621L). The retroviral construct containing farnesylation signal-GFP was purchased from addgene (21836) ...
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bioRxiv - Cancer Biology 2023Quote: ... and concentration determined using NEBNext Lib Quant Kit (E7630, NEB). Input and pulldown libraries were sequenced with an Illumina NextSeq550 with 42bp per read using the NextSeq 500/550 High Output 75-cycle v2.5 kit.
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bioRxiv - Cancer Biology 2023Quote: ... and concentration determined using NEBNext Lib Quant Kit (E7630, NEB). Libraries were sequenced with an Illumina NextSeq550 with 42bp per read (total of 84bp ...
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bioRxiv - Cancer Biology 2023Quote: ... and concentration determined using NEBNext Lib Quant Kit (E7630, NEB). Libraries were sequenced with an Illumina NextSeq550 with 42bp per read (total of 84bp ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The ProtoScript First Strand cDNA Synthesis Kit (New England Biolabs) was used for the synthesis of cDNA using the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... Using the NEBNext Ultra II DNA library prep kit (NEB), the DNA was adaptor-ligated and indexed for Illumina NGS sequencing ...
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bioRxiv - Bioengineering 2023Quote: sgRNA was synthesized using the EnGen sgRNA synthesis kit (NEB). Target specific oligos (Table S4 ...
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bioRxiv - Cancer Biology 2023Quote: ... and concentrations determined using NEBNext Lib Quant Kit (E7630, NEB). Libraries were sequenced on an Illumina NextSeq 550 ...
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bioRxiv - Genomics 2023Quote: ... the Monarch® Genomic DNA Purification Kit (NEB, Cat# T3010) was used (10 µl Proteinase K ...
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bioRxiv - Biophysics 2024Quote: ... All mutations were constructed by using Gibson assembly kit (NEB). The signal peptide of murine immunoglobulin κ chain was used for expression of PDGFRβ and CSF-1R receptors ...
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bioRxiv - Genomics 2024Quote: ... and purified using a Monarch RNA Cleanup Kit (NEB, T2040S), both according to the Manufacturer’s protocols ...
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bioRxiv - Biophysics 2024Quote: ... purified using a PCR clean up kit (NEB, Cat# T1030L) and eluted in water ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was generated by using LunaScript RT SuperMix Kit (NEB) and qPCR was performed with the Luna universal qPCR Master Mix (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... The plasmids were extracted using Monarch Plasmid Miniprep kit (NEB) and precipitated by cold ethanol ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids were assembled using NEBuilder HiFi DNA assembly kit (NEB). DNA was quantified on a Nanodrop Lite or Qubit HS DNA or RNA kit (Thermo) ...
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bioRxiv - Neuroscience 2024Quote: ... Ribosomal RNA was removed using the rRNA Depletion kit (NEB) and multiplexed RNA-seq libraries were generated using the NEBNext Ultra Directional RNA library Prep Kit for Illumina (NEB) ...
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bioRxiv - Developmental Biology 2024Quote: ... using the Q5 site-directed mutagenesis kit (New England Biolabs). These substitutions did not alter protein sequences ...
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bioRxiv - Developmental Biology 2024Quote: ... and purified using Monarch® RNA Cleanup Kit (NEB, T2040). An RNA/DNA mix was obtained by retrotranscribing 200pmol of RNA + 200pmol of the Reserve primer (5’-TTGTCGCACGTTCGGTGTCG-3’ ...
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bioRxiv - Microbiology 2024Quote: ... and purified with the Monarch RNA clean-up kit (NEB). cDNA was prepared from 1 μg of RNA ...
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bioRxiv - Molecular Biology 2024Quote: ... mRNA was isolated using the mRNA Isolation kit (NEB, S1550S). mRNA was fragmented using the NEBNext Magnesium RNA fragmentation module (NEB ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids were assembled using NEBuilder HiFi DNA assembly kit (NEB). DNA was quantified on a Nanodrop Lite or Qubit ...
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bioRxiv - Neuroscience 2024Quote: ... and hybridized with NEBNext rRNA Depletion Kit v2 (NEB E7400) to substantially diminish rRNA from the samples ...
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bioRxiv - Developmental Biology 2024Quote: ... Libraries were quantitated using NEBNext Library Quant Kit (NEB, E7630,). For RNA sequencing ...
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bioRxiv - Genomics 2024Quote: ... and library amplification using Phusion HF PCR kit (NEB #E0553L).
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bioRxiv - Plant Biology 2024Quote: ... cDNA was synthesised using the LunaScript kit (New England Biolabs). PCR amplification of WLV was done using PuReTag Ready-to-Go PCR beads (Illustra ...
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bioRxiv - Cell Biology 2024Quote: ... total RNA was isolated using Monarch RNA prep kit (NEB). Reverse transcription was performed on 0.5µg DNase-treated total RNA using Lunascript RT (NEB ...
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bioRxiv - Developmental Biology 2024Quote: ... the NEBNext Poly(A) mRNA Magnetic Isolation Kit (NEB, E7490) was employed to capture mRNA ...
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bioRxiv - Neuroscience 2024Quote: ... with NEB Next Library Quant Kit for Illumina (NEB, E7630L) and KAPA Library Quantification Kit (Roche ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We used the Monarch PCR and DNA Cleanup Kit (NEB) to purify PCR products ...
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bioRxiv - Genetics 2024Quote: ... Libraries were prepared using Ultra II RNA library kit (NEB) and samples were sequenced on the NovaSeq 6000 platform (Illumina ...
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bioRxiv - Plant Biology 2024Quote: ... 5 and 7dpi using a total RNA extraction kit (NEB) and following the kit instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The NebNext® UltraTM II DNA library prep kit (NEB) for Illumina was used for sequencing library preparation following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were prepared according to manufacturer’s instructions using the following kits: NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® and NEBNext® Multiplex Oligos for Illumina® (New England BioLabs) and pooled in equimolar quantities ...
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bioRxiv - Molecular Biology 2022Quote: ... Ribosomal RNA was depleted of using the NEBNext® rRNA Depletion Kit and cDNA libraries were prepared using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich MA). RNA was barcoded using the NEBNext Multiplex Oligos for Illumina (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... or a region in the N-terminal part (for TGDs) were PCR amplified (see Table S4 for primer sequences) from 3D7 or IT4 gDNA (Monarch gDNA Purification Kit, NEB or QIAGEN DNA extraction kit) and cloned into pSLI (Birnbaum et al. ...
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bioRxiv - Molecular Biology 2020Quote: Cleavage of the MBP tag from NlGr7 was completed according to manual instructions of the pMAL protein fusion and purification system (NEB, Inc, USA). The tag (MBP ...
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bioRxiv - Molecular Biology 2020Quote: ... the desired sequences pertaining to the truncated forms of the N protein were subcloned from the synthetic gene using polymerase chain reaction (Phusion polymerase, New England Biolabs, Hertfordshire, UK). All genes were cloned into the modified pet28 vector and gene sequences were confirmed by Sanger Sequencing.
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bioRxiv - Biochemistry 2022Quote: ... purified Trx-FER-KD and FER-KDK565R proteins were treated with 1 μl of λ-phosphatase (λ-PP) (400,000 units/ml, New England Biolabs. P0753S) and 1 mM MnCl2 for 1-2 h at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... protease mutant S219V [24] was inserted at the 3’ end of gene encoding maltose binding protein (MBP) in pMAL-c5E vector (New England Biolabs, MA, USA) to construct pMAL-TEV vector ...