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Citations for New England Biolabs :
2201 - 2250 of 3093 citations for African Swine Fever Virus P72 Protein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... The vimentin constructs (Y117L mutants and addition of a Spot tag) were produced using a Q5 Site-Directed Mutagenesis kit (Biolabs cat # E0554S) using the primer lists in Table 1 ...
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bioRxiv - Microbiology 2024Quote: ... and assembled in frame into the digested pLIC-HA -DHFR-TS plasmid in frame with the 3x HA tag using the NEBuilder HiFi DNA Assembly cloning Kit (NEB, cat# E5520) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... the Strep-tag® II sequence was inserted at the C-terminus via Phusion® High-Fidelity DNA Polymerase mutagenesis PCR (New England Biolabs) using the primers pYS14_GA_F/ 5’ phosphorylated flaakstrp14R to amplify the whole plasmid ...
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bioRxiv - Molecular Biology 2023Quote: ... This sequence was inserted into a modified pST50 vector56 containing N-terminal Strep-6xHis-TEV-GFP-PreScission tags using Gibson assembly (NEB Cat #: E2611L) to obtain a tagged construct of full-length HURP ...
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Structural and mechanistic insights into disease-associated endolysosomal exonucleases PLD3 and PLD4bioRxiv - Biochemistry 2023Quote: ... Lentiviral related PLD3/4 plasmids were cloned into pBOBI vector that has a C-terminal FLAG tag after digestion by restriction enzymes BamHI and XhoI (NEB R3136S, R0146S). All these plasmids were prepared with endotoxin-free kit (Takara 740426 ...
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bioRxiv - Molecular Biology 2024Quote: ... and cloned into overexpression vectors under act5 promoter with either N-terminal or C-terminal FLAG tags using Gibson Assembly® Master Mix (NEB, E2611L) at 50 °C for 1 hr ...
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bioRxiv - Immunology 2021Quote: Restriction digest of pEX-K4 plasmid DNA containing EtAMA1Cit or was performed using Bam HI and Xho I (New England Biolabs, Ipswich, MA, USA) to extract tagged antigen coding sequences for cloning into pYD1 plasmid vector ...
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bioRxiv - Biochemistry 2024Quote: N-term-His-ELMO1C438A construct was generated from N-term-His-ELMO1 construct obtained above using Q5® Site-Directed Mutagenesis Kit (New England BioLabs, cat # E0554S), with the following primers.
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bioRxiv - Biophysics 2024Quote: The mini-Lpd (his-Z-EGFP-LZ-Lpd(aa850-1250)) and monomeric-mini-Lpd (his-EGFP-Lpd(aa850-1250)) were transformed into BL21 (NEB, cat. No. C2527H) and grown at 30 °C to an OD of 0.8 ...
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bioRxiv - Plant Biology 2021Quote: ... The immunoprecipitated protein was incubated with λ protein phosphatase using manufacturer’s protocol (Cat. # P0753S, NEB). The treated samples were resolved on SDS-PAGE and western blotting performed using OsFD7 antibody.
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bioRxiv - Plant Biology 2022Quote: ... The protein extracts were incubated with Lambda Protein Phosphatase (λ-PPase) (NEB, Cat. No. P0753S) at 30 □ for 30 min and subsequently added 5×SDS sample buffer incubated at 95 □ for 5 min ...
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bioRxiv - Biochemistry 2019Quote: ... Cas12a were synthesized as Escherichia coli codon-optimized gBlocks (purchased from Integrated DNA Technologies, IDT) and inserted into pSV272 using Gibson assembly (New England Biolabs) as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... were expressed with an N-terminal His8 tag followed by a 3C protease site from a pBAD-TOPO derived plasmid in Escherichia coli ER2566 cells (New England Biolabs, fhuA2 lacZ::T7 gene1 [lon] ompT gal sulA11 R(mcr73::miniTn10--TetS)2 [dcm] R(zgb-210::Tn10--TetS ...
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bioRxiv - Biochemistry 2021Quote: ... coli or Expi293™ cells underwent denaturation at 95 °C for 10 min in glycoprotein denaturing buffer (New England Biolabs) followed by immediate cooling on ice for 10 s ...
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bioRxiv - Microbiology 2022Quote: ... The ravA and viaA genes were PCR amplified from Escherichia coli K-12 MG1655 genomic DNA using Phusion polymerase (Biolabs). All PCR products were purified by DNA cleanup kit (Qiagen) ...
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bioRxiv - Developmental Biology 2022Quote: ... coli and by performing heat shock for 30 seconds at 42°C followed by recovery in SOC outgrowth medium (NEB) for 1 hour ...
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bioRxiv - Genetics 2019Quote: ... All the plasmids used in this study (Supplementary Table S3, doi:10.6084/m9.figshare.7752458) were propagated in Escherichia coli DH5α cells (NEB, UK) by growing cells in LB media without NaCl (Formedium ...
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bioRxiv - Plant Biology 2022Quote: ... The fragments were purified and inserted into pCAMBIA3300U (pCAMBIA3300 with a double PacI USER cassette inserted between the PstI-XmaI sites at the multiple cloning site) (Nour-Eldin et al, 2006) after which the plasmid was transformed into Escherichia coli DH5α (NEB). Kanamycin resistant colonies were selected and their plasmids purified followed by restriction digestion and sequencing prior to introduction into Agrobacterium tumefaciens GV3101 for plant transformation ...
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bioRxiv - Molecular Biology 2022Quote: ... The latter gene was Phusion-PCR amplified from pLemo isolated from Escherichia coli Lemo(DE3) cells (New England Biolabs, USA) using a set of primers annealing right in front of and behind the ORF of this gene (primers used ...
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bioRxiv - Biochemistry 2023Quote: ... the MsbA gene (from Escherichia coli genomic DNA) was amplified by polymerase chain reaction (PCR) using Q5 High-Fidelity DNA Polymerase (New England Biolabs, NEB) and subcloned into a modified pCDF-1b plasmid (Novagen ...
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bioRxiv - Molecular Biology 2023Quote: ... these antibiotic resistant cassettes were excised by transforming the strains with a plasmid expressing the Cre recombinase (pDR244, BGSCID: ECE274) purified from RecA+ Escherichia coli (NEB) cells with the GeneJET Plasmid Miniprep Kit (Thermo) ...
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bioRxiv - Molecular Biology 2023Quote: The bacterial source strains used for this work (Table S3) were Listeria monocytogenes LL195 as a main infection model and Escherichia coli NEB5α (New England BioLabs) for protein purification ...
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bioRxiv - Biochemistry 2022Quote: ... coli cells (α-select, XL1-Blue, or BL21-DE3 cells were used, depending on application; all originally sourced from NEB and obtained from lab made stocks ...
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bioRxiv - Cell Biology 2022Quote: ... coli cells (α-select, XL1-Blue, or BL21-DE3 cells were used, depending on application; all originally sourced from NEB) for 30 min on ice ...
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bioRxiv - Biochemistry 2023Quote: ... N-terminally hexahistidine-tagged Sfr1 was co-expressed with Swi5 as an operon from a pET11b vector in an Escherichia coli BL21 DE3 strain (NEB) that had been pre-transformed with the pRARE2 plasmid (Novagen) ...
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bioRxiv - Plant Biology 2023Quote: The ZmCKX5 gene (Phytozome ID Zm00001d008862) was cloned into a pTYB12 vector and expressed in T7 Express Escherichia coli cells (New England Biolabs) at 18 °C overnight ...
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bioRxiv - Genomics 2019Quote: RNA libaries were prepared using both E7530L and E&335L NEBNext® Ultra™ RNA Library Prep Kits (New England Biolabs, USA) to generate fragment sizes of 351-371 bp ...
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bioRxiv - Microbiology 2022Quote: DNA from two samples (R-F1-E and S-F3-N) were used for preparing DNA metagenome libraries using NEBNext Ultra DNA Library Prep Kit (NEB, Ipswich, MA) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and PCR-amplified XTEN80-mEGFP-3xHA immunoprecipitation tag with a linearized backbone generated from ClaI and BspDI (New England BioLabs cat. no. R0557) digestion of pKL017 (pHIV EF1a:Clover:WPRE) ...
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bioRxiv - Microbiology 2022Quote: ... repeat ΔEC4 (residues 328-446) were synthesized in frame with Myc and Flag epitope tags at the C-terminus (Epoch Biolabs or Twist Bioscience) and cloned into the pBABE-puro retroviral vector (58) ...
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bioRxiv - Cell Biology 2023Quote: ... and 62.5 μL of Lambda Protein Phosphatase (New England Biolabs, 5 U μg-1 of protein) were added with gentle mixing after each addition ...
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bioRxiv - Molecular Biology 2022Quote: ... dsRNA was synthesized from T7-linked DNA using the HI Scribe™ T7 High Yield RNA Synthesis Kit (New England, Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... 1200 units λ-protein phosphatase (NEB) were diluted into phosphatase assay buffer without cell lysate ...
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bioRxiv - Microbiology 2021Quote: ... Stained and unstained protein ladders (NEB; #P7719S and #P7717S ...
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bioRxiv - Molecular Biology 2019Quote: ... 200 units λ protein phosphatase (NEB) was added to 10 μL of the suspension ...
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bioRxiv - Molecular Biology 2020Quote: ... 1uL of lambda protein phosphatase (NEB) was added to samples ...
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bioRxiv - Bioengineering 2021Quote: ... 90 nM of SpCas9 protein (NEB), NEB buffer 3.1 (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... and Cas9 protein (NEB - 0.3 μM). This was incubated at 37°C for 10 minutes before microinjection ...
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bioRxiv - Genetics 2022Quote: ... and Cas9 protein (NEB - 0.3 μM). This was incubated at 37°C for 10 minutes before microinjection ...
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bioRxiv - Microbiology 2022Quote: ... Color Prestained Protein Standard (NEB, P7719) or Precision Plus Protein All Blue Prestained Protein Standards (BioRad ...
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bioRxiv - Microbiology 2020Quote: ... proteins were deglycosylated by PNGaseF (NEB) prior to gel electrophoresis ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Cas9 protein was purchased from NEB EnGen Cas9 NLS ...
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bioRxiv - Genomics 2024Quote: ... 90 nM of SpCas9 protein (NEB), NEB buffer 3.1 (NEB ...
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bioRxiv - Microbiology 2023Quote: Protein expression vector pMAL-c5Xa (NEB) was used as the backbone for constructing the mCherry reporter ...
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bioRxiv - Biochemistry 2022Quote: ... Protein markers (New England Biolabs, #P7719S) were used for molecular mass determination.
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bioRxiv - Microbiology 2023Quote: ... proteins were deglycosylated by PNGaseF (NEB) prior to gel electrophoresis to facilitate analysis.
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bioRxiv - Cell Biology 2023Quote: ... and Protein Kinase A (NEB, P6000S) were purchased from New England Biolabs ...
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bioRxiv - Bioengineering 2024Quote: ... including protein disulfide bond enhancer (NEB) and GamS (NEB) ...
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bioRxiv - Genetics 2024Quote: ... 2 µl Cas9 Protein (M0386, NEB), 0.5µl buffer (NEB) ...
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bioRxiv - Genetics 2019Quote: ... coli was performed using DH5-alpha or its commercial derivative NEB 10-Beta (New England Biolabs product number C3019I or C3020). Genomic DNA of BY4741 ...